Click here to switch to the map view.

The map label for this gene is thrB

Identifier: 21674844

GI number: 21674844

Start: 1932560

End: 1933534

Strand: Direct

Name: thrB

Synonym: CT2034

Alternate gene names: 21674844

Gene position: 1932560-1933534 (Clockwise)

Preceding gene: 21674841

Following gene: 21674845

Centisome position: 89.68

GC content: 59.28

Gene sequence:

>975_bases
ATGAAAACCGTCACTGGATTTGCTTCAGCAACGGTTGGAAACGTTGCCTGCGGATTTGACGTCCTCGGTTTCGCCATCAC
CGAACCTGGAGACGAGGTCGTTCTGGCGCTTCACGATGAGCGCAGAAGCGACTGCCCCGTTTCGATCACCTCGATTGTGG
GTGACGGCGGAGCCTTGCCGCTCGACCCGAAAAAAAATACTTCGAGCTTCGTTGTCCTCAAGTTCCTTGAATATATCCGC
ACCACGAAAGGCATCTCGTTTGACGGTCACATCGATCTGGTGCTTAAAAAAAATCTGCCACTCTCCAGCGGCATGGGCAG
CAGCGCGGCAAGCGCAGCGGCGGCACTGATTGCCGCCAATGAGCTGTTCGGCTCGCCCTGCACCAAAATGGAGCTGGTAC
ACTTCGCCATCGAGGGCGAGCGCGTCGCCTGCGGTTCGGCCCATGCCGACAATGCCGCTCCGGCCATGCTCGGCAACTTC
ATCCTGATCCGCAGCTACAACCCGCTCGACCTGATCACCATCAAACCACCAAAAAATCTTTTCGGCACACTCGTGCATCC
GCACACCGAACTCAAAACCTCGTTCGCCCGCTCCGTGCTGCCGAAATCGATTCCGCTTTCGACCGCCACACAGCAGTGGG
GCAACGTCGGCGCACTCATCGCCGGCCTGTTGATGGAGGATTACGACCTGATCGGACGGGCGCTGGTGGATGTGGTCGCC
GAGCCAAAACGCGCACCGCTCATTCCGGGATTCAACGAGGTCAAACAGGCCGCGCTCGACGCGGGAGCTCTCGGTTGCAG
CATTGCCGGATCCGGACCGTCAGTTTTCGCCTTCTCCTCGTCCAGGCAAACCGCAGAAGCGGTGGGCTCGGCCATGCAGT
CGGCGTTCCTGCACTCAAGGGCTGCACTCCAGTCGGACATGTGGGTCTCGCCGATTTGCAGCCAGGGCGCACGCATCATC
AGCACAACTTCTTGA

Upstream 100 bases:

>100_bases
AGCATTCAGTTATTCAAATGCCAGAACCAATTTGGATAATCATCGTTTCCAGACTGCTACGCGACCAGACCAAATCAGGA
ATCTTTCCCCAAATCACGCT

Downstream 100 bases:

>100_bases
AACGCATCACGATATGATCTTCTACAGCACGACGAAAGCGTCTGCTCCCGTCACGATGAAAAAAGCCACCCTCGAAGGGC
TCGCACCCGATGGAGGGTTG

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKTVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDGGALPLDPKKNTSSFVVLKFLEYIR
TTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANELFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNF
ILIRSYNPLDLITIKPPKNLFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRALVDVVA
EPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSRAALQSDMWVSPICSQGARII
STTS

Sequences:

>Translated_324_residues
MKTVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDGGALPLDPKKNTSSFVVLKFLEYIR
TTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANELFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNF
ILIRSYNPLDLITIKPPKNLFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRALVDVVA
EPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSRAALQSDMWVSPICSQGARII
STTS
>Mature_324_residues
MKTVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDGGALPLDPKKNTSSFVVLKFLEYIR
TTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAANELFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNF
ILIRSYNPLDLITIKPPKNLFGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRALVDVVA
EPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSRAALQSDMWVSPICSQGARII
STTS

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=321, Percent_Identity=29.9065420560748, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_CHLTE (Q8KAW7)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662909.1
- ProteinModelPortal:   Q8KAW7
- SMR:   Q8KAW7
- GeneID:   1007526
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2034
- NMPDR:   fig|194439.1.peg.2003
- TIGR:   CT2034
- HOGENOM:   HBG646290
- OMA:   GSAHADN
- ProtClustDB:   PRK01212
- BioCyc:   CTEP194439:CT_2034-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 33771; Mature: 33771

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDGGALP
CCCCCCCHHHHHCCHHHCHHHHEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCCCCCC
LDPKKNTSSFVVLKFLEYIRTTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAAN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNFILIRSYNPLDLITIKPPKNL
HHHCCCCCCEEEEEEEECCCEEEECCCCCCCCCHHHHCCEEEEEECCCEEEEEECCCHHH
FGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRALVDVVA
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
EPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSR
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AALQSDMWVSPICSQGARIISTTS
HHHHCCCCCCHHHHCCCEEEEECC
>Mature Secondary Structure
MKTVTGFASATVGNVACGFDVLGFAITEPGDEVVLALHDERRSDCPVSITSIVGDGGALP
CCCCCCCHHHHHCCHHHCHHHHEEEEECCCCEEEEEEECCCCCCCCEEEEEEECCCCCCC
LDPKKNTSSFVVLKFLEYIRTTKGISFDGHIDLVLKKNLPLSSGMGSSAASAAAALIAAN
CCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFGSPCTKMELVHFAIEGERVACGSAHADNAAPAMLGNFILIRSYNPLDLITIKPPKNL
HHHCCCCCCEEEEEEEECCCEEEECCCCCCCCCHHHHCCEEEEEECCCEEEEEECCCHHH
FGTLVHPHTELKTSFARSVLPKSIPLSTATQQWGNVGALIAGLLMEDYDLIGRALVDVVA
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHC
EPKRAPLIPGFNEVKQAALDAGALGCSIAGSGPSVFAFSSSRQTAEAVGSAMQSAFLHSR
CCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHH
AALQSDMWVSPICSQGARIISTTS
HHHHCCCCCCHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901