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The map label for this gene is thrA [H]

Identifier: 21674840

GI number: 21674840

Start: 1927055

End: 1929511

Strand: Reverse

Name: thrA [H]

Synonym: CT2030

Alternate gene names: 21674840

Gene position: 1929511-1927055 (Counterclockwise)

Preceding gene: 21674842

Following gene: 21674839

Centisome position: 89.54

GC content: 60.44

Gene sequence:

>2457_bases
ATGAGGGTATTCAAGTTCGGAGGTTCGTCGATCGCTTCGGCGGCAAAGATCAGCAATGTGGCTGGCATTATCAGGCGTGA
GTTGAAAAGCACTCCGCTGGTGGTTGTGGTGTCAGCGATTGGCAAGGTGACCGACATGCTTGCCGAGACTGCGGCGCTGG
CGGGGAATGGTGATGCGGCATACCGCGATAAACTCGAAGGAATCGCCTCGCTGCATGGCGGGATCATCCGTGAACTGTTC
GGTACGGAGGCGTCAGCAGAAGAGACCTGGTTGGGTGAGATGATGGCTGAACTGAACGATGTGCTGCATGGCGTTTTTCT
GCTCAGGGAGCTTTCAGACAAGAGTCTCGCCCTTGTGCTGAGTTACGGCGAACGGCTGTCGTGCCGGATCGTGAGCCGCT
ATATGCACGTATCGGGTACCCCAGCTGAATGCGTCGATGCACGCAGTGTGATCGTGACCGACGACAACCATTGCTTTGCC
AAGGTGGATCGTCTGGCGACAGGAAAGCTGATTCACGAACGCTTCCGGAGTTTCGACGTTCTGCCGGTGGTCACCGGATT
CATCGCCTCCGCGCCGGATGGCAGTGTGACCAACCTTGGGCGCGGCGGTTCCGATTTCACGGCGACGATTCTCGGCGCGG
CGCTTCATGCCGAAGAGGTGTGGATCTGGACCGATGTCGATGGCTTCTACAGCGCCGATCCCAAGCGCGTGCCCGACGCG
CGGGTCATTCCGGAGATCAGCTACGCCGAGGCGATGGAGCTGTCGCACGCCGGCGCGAAGGTGCTGCATCCACTGGCCGT
GCAGCCGGTCATGAAGGCGGGTATTCCGTTGCGCATAAAGAACTCTTTCAATCCCGAAAAACCTGGCACGAGGATCGGTA
TCGAGGCTGCGGGTGCAGAGGCGCTTCCGGGTACGGTGACCGGACTGACCTCGATCAACCATGTGGTGCTGCTCAGTCTT
TCCGGCAGCGGCATGGCGGGCGTGCCGGGAACGGCGTCTCGGCTGTTTACCTGCCTCGCTCGCCACAGCATCAACATTAT
CTTCATCTCGCAGGCCTCGTCGGAGCAGTCGATCAGCCTGGCCATAGCGCCCGGACAGGCGTCGATGGCCAAGAAGGTGC
TCGAAGAGGAGTTTGCCCGCGAAATCGAGGAGCGCCGGATCGATCCGGTCAGCGTCCGCCGCAATCTTGCGATGGTGGCC
GTGGTGGGCAATAAGATGTTGGGCCACCCCGGCGTGTCGGCACAGCTCTTCGAGACGCTCGGCAAGAACGGCGTCAACGT
GATCGCCGTCGCGCAGGGGGCCAACGAAATGAACATTTCGCTGGTCATCGACAGCGCGGATGAGAACAAGGCGCTCAACT
GCGTCCACGAATCCTTCTTCCTTTCGATGCGCAAGGTGCACGTTTTCATCGTGGGTACCGGCACCATCGCCAAAAGTCTC
ATCAGCCAGATCCGGCGGCATCGCGCCACCCTGCAGAAAGAATTGGGCCTTGACGTTGTGGTTGCGGGTCTGGCCAACAC
CCGCTCGATGTGCATCGAGCCTGCCGGAATCGATCTCGAACACTGGCACGATTCGCTGAAGCCGCGCGAGTCGCACGAGG
GGATCGGGCAGTATATCCGTCTGATTCAGGAACGGAACCTGCACAACACCATCGTTGTGGACTGTACGGCGAGCAGGCAG
GTGGCCGAGTGCTACCCCGCGCTGCTCCGGGCGAATATCTCTGTTGCCACGGCCAACAAACTTGGCATGGCTGGGCCGTG
GGATCTGTATCGCAAGATCATGGATGCGTTACGCTCCAGTAATGCGAAGTTCCTCTACGAAACCAATGTTGGGGCGGGTT
TGCCGGTGATCAACACGCTTAACGACCTGAAGAACAGCGGAGACAAGATCGTCTGCATCGAGGGCGTTTTGTCGGGTACA
CTAAGCTTCATTTTCAACGAGCTGCGCAAGGGCGGGCGTTTCAGCGAGATCGTTCGCAAAGCCAAGGAGTCTGGCTACAC
CGAGCCCGATCCGCGCGATGATCTCTCCGGAGCCGATTTCGCCCGGAAGCTGCTTATTCTCGGTAGGGAGCTTGGCTATC
AGCTCGAGTACGCGGACGTGGAGTGCCAGAGCCTCGTCCCGGAGCCGCTGCGAGGCGAAATGTCGCCCGCCGAGTTTCTC
GACCAGCTCTCTTCAATCGACGACTGGTACGTCGATGAGATGGCGAGTGCCGCGAGCGAGGGGATGACCATCGCCTACAC
CGGCGAGCTTCGGGACGGCAAGGCGAAGGTGGGGCTGAAGCGCGTGCCGCTCGAAAGCCCGGTGGCCGGGCTGAACGGCT
CGGAGAACCTCGTGGTTTTCACGACCGAGCGCTACCTCAAGACCCCGCTGGTGGTCAAGGGGCCTGGCGCTGGCGGCGAG
GTGACAGCAGGCGGCGTCTTCGCCGACATTCTCCGCATCGCAAGCTATTTGGTCTGA

Upstream 100 bases:

>100_bases
CAAAACTTCTGGAAAAAGAGCATTTCCCGGTTTGTTTTATTGCCCTGCTCGTAAGAAATTACAAAGACAAGTTTAATCGT
GTAATGCAGCAAGGTTGAAG

Downstream 100 bases:

>100_bases
GTCGCCGGCATGAAAGCAATCATCATCTCTATCGGCGACGAGTTGCTCAAGGGGCACCGGGTCAACACCAACGCGCCCTT
TATCGCTCGTGAACTCGGCA

Product: bifunctional aspartokinase I/homeserine dehydrogenase I

Products: NA

Alternate protein names: Aspartokinase I/homoserine dehydrogenase I; AKI-HDI; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 818; Mature: 818

Protein sequence:

>818_residues
MRVFKFGGSSIASAAKISNVAGIIRRELKSTPLVVVVSAIGKVTDMLAETAALAGNGDAAYRDKLEGIASLHGGIIRELF
GTEASAEETWLGEMMAELNDVLHGVFLLRELSDKSLALVLSYGERLSCRIVSRYMHVSGTPAECVDARSVIVTDDNHCFA
KVDRLATGKLIHERFRSFDVLPVVTGFIASAPDGSVTNLGRGGSDFTATILGAALHAEEVWIWTDVDGFYSADPKRVPDA
RVIPEISYAEAMELSHAGAKVLHPLAVQPVMKAGIPLRIKNSFNPEKPGTRIGIEAAGAEALPGTVTGLTSINHVVLLSL
SGSGMAGVPGTASRLFTCLARHSINIIFISQASSEQSISLAIAPGQASMAKKVLEEEFAREIEERRIDPVSVRRNLAMVA
VVGNKMLGHPGVSAQLFETLGKNGVNVIAVAQGANEMNISLVIDSADENKALNCVHESFFLSMRKVHVFIVGTGTIAKSL
ISQIRRHRATLQKELGLDVVVAGLANTRSMCIEPAGIDLEHWHDSLKPRESHEGIGQYIRLIQERNLHNTIVVDCTASRQ
VAECYPALLRANISVATANKLGMAGPWDLYRKIMDALRSSNAKFLYETNVGAGLPVINTLNDLKNSGDKIVCIEGVLSGT
LSFIFNELRKGGRFSEIVRKAKESGYTEPDPRDDLSGADFARKLLILGRELGYQLEYADVECQSLVPEPLRGEMSPAEFL
DQLSSIDDWYVDEMASAASEGMTIAYTGELRDGKAKVGLKRVPLESPVAGLNGSENLVVFTTERYLKTPLVVKGPGAGGE
VTAGGVFADILRIASYLV

Sequences:

>Translated_818_residues
MRVFKFGGSSIASAAKISNVAGIIRRELKSTPLVVVVSAIGKVTDMLAETAALAGNGDAAYRDKLEGIASLHGGIIRELF
GTEASAEETWLGEMMAELNDVLHGVFLLRELSDKSLALVLSYGERLSCRIVSRYMHVSGTPAECVDARSVIVTDDNHCFA
KVDRLATGKLIHERFRSFDVLPVVTGFIASAPDGSVTNLGRGGSDFTATILGAALHAEEVWIWTDVDGFYSADPKRVPDA
RVIPEISYAEAMELSHAGAKVLHPLAVQPVMKAGIPLRIKNSFNPEKPGTRIGIEAAGAEALPGTVTGLTSINHVVLLSL
SGSGMAGVPGTASRLFTCLARHSINIIFISQASSEQSISLAIAPGQASMAKKVLEEEFAREIEERRIDPVSVRRNLAMVA
VVGNKMLGHPGVSAQLFETLGKNGVNVIAVAQGANEMNISLVIDSADENKALNCVHESFFLSMRKVHVFIVGTGTIAKSL
ISQIRRHRATLQKELGLDVVVAGLANTRSMCIEPAGIDLEHWHDSLKPRESHEGIGQYIRLIQERNLHNTIVVDCTASRQ
VAECYPALLRANISVATANKLGMAGPWDLYRKIMDALRSSNAKFLYETNVGAGLPVINTLNDLKNSGDKIVCIEGVLSGT
LSFIFNELRKGGRFSEIVRKAKESGYTEPDPRDDLSGADFARKLLILGRELGYQLEYADVECQSLVPEPLRGEMSPAEFL
DQLSSIDDWYVDEMASAASEGMTIAYTGELRDGKAKVGLKRVPLESPVAGLNGSENLVVFTTERYLKTPLVVKGPGAGGE
VTAGGVFADILRIASYLV
>Mature_818_residues
MRVFKFGGSSIASAAKISNVAGIIRRELKSTPLVVVVSAIGKVTDMLAETAALAGNGDAAYRDKLEGIASLHGGIIRELF
GTEASAEETWLGEMMAELNDVLHGVFLLRELSDKSLALVLSYGERLSCRIVSRYMHVSGTPAECVDARSVIVTDDNHCFA
KVDRLATGKLIHERFRSFDVLPVVTGFIASAPDGSVTNLGRGGSDFTATILGAALHAEEVWIWTDVDGFYSADPKRVPDA
RVIPEISYAEAMELSHAGAKVLHPLAVQPVMKAGIPLRIKNSFNPEKPGTRIGIEAAGAEALPGTVTGLTSINHVVLLSL
SGSGMAGVPGTASRLFTCLARHSINIIFISQASSEQSISLAIAPGQASMAKKVLEEEFAREIEERRIDPVSVRRNLAMVA
VVGNKMLGHPGVSAQLFETLGKNGVNVIAVAQGANEMNISLVIDSADENKALNCVHESFFLSMRKVHVFIVGTGTIAKSL
ISQIRRHRATLQKELGLDVVVAGLANTRSMCIEPAGIDLEHWHDSLKPRESHEGIGQYIRLIQERNLHNTIVVDCTASRQ
VAECYPALLRANISVATANKLGMAGPWDLYRKIMDALRSSNAKFLYETNVGAGLPVINTLNDLKNSGDKIVCIEGVLSGT
LSFIFNELRKGGRFSEIVRKAKESGYTEPDPRDDLSGADFARKLLILGRELGYQLEYADVECQSLVPEPLRGEMSPAEFL
DQLSSIDDWYVDEMASAASEGMTIAYTGELRDGKAKVGLKRVPLESPVAGLNGSENLVVFTTERYLKTPLVVKGPGAGGE
VTAGGVFADILRIASYLV

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=820, Percent_Identity=37.4390243902439, Blast_Score=555, Evalue=1e-159,
Organism=Escherichia coli, GI1790376, Length=828, Percent_Identity=30.6763285024155, Blast_Score=338, Evalue=1e-93,
Organism=Escherichia coli, GI1790455, Length=470, Percent_Identity=29.3617021276596, Blast_Score=145, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6320893, Length=497, Percent_Identity=29.5774647887324, Blast_Score=197, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6322599, Length=364, Percent_Identity=32.6923076923077, Blast_Score=168, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 87916; Mature: 87916

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE ; PS00178 AA_TRNA_LIGASE_I ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVFKFGGSSIASAAKISNVAGIIRRELKSTPLVVVVSAIGKVTDMLAETAALAGNGDAA
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHH
YRDKLEGIASLHGGIIRELFGTEASAEETWLGEMMAELNDVLHGVFLLRELSDKSLALVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SYGERLSCRIVSRYMHVSGTPAECVDARSVIVTDDNHCFAKVDRLATGKLIHERFRSFDV
ECCCCHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCH
LPVVTGFIASAPDGSVTNLGRGGSDFTATILGAALHAEEVWIWTDVDGFYSADPKRVPDA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCC
RVIPEISYAEAMELSHAGAKVLHPLAVQPVMKAGIPLRIKNSFNPEKPGTRIGIEAAGAE
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCC
ALPGTVTGLTSINHVVLLSLSGSGMAGVPGTASRLFTCLARHSINIIFISQASSEQSISL
CCCCCHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
AIAPGQASMAKKVLEEEFAREIEERRIDPVSVRRNLAMVAVVGNKMLGHPGVSAQLFETL
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEECCHHCCCCCCHHHHHHHH
GKNGVNVIAVAQGANEMNISLVIDSADENKALNCVHESFFLSMRKVHVFIVGTGTIAKSL
CCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEEEEEEEEEECHHHHHHH
ISQIRRHRATLQKELGLDVVVAGLANTRSMCIEPAGIDLEHWHDSLKPRESHEGIGQYIR
HHHHHHHHHHHHHHCCCCEEEECCCCCCHHEECCCCCCHHHHHHCCCCCHHHHHHHHHHH
LIQERNLHNTIVVDCTASRQVAECYPALLRANISVATANKLGMAGPWDLYRKIMDALRSS
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCC
NAKFLYETNVGAGLPVINTLNDLKNSGDKIVCIEGVLSGTLSFIFNELRKGGRFSEIVRK
CCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKESGYTEPDPRDDLSGADFARKLLILGRELGYQLEYADVECQSLVPEPLRGEMSPAEFL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCCCCCCCHHHHH
DQLSSIDDWYVDEMASAASEGMTIAYTGELRDGKAKVGLKRVPLESPVAGLNGSENLVVF
HHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHCCEEECCCCCCCCCCCCCCCEEEE
TTERYLKTPLVVKGPGAGGEVTAGGVFADILRIASYLV
EEHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVFKFGGSSIASAAKISNVAGIIRRELKSTPLVVVVSAIGKVTDMLAETAALAGNGDAA
CEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHH
YRDKLEGIASLHGGIIRELFGTEASAEETWLGEMMAELNDVLHGVFLLRELSDKSLALVL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SYGERLSCRIVSRYMHVSGTPAECVDARSVIVTDDNHCFAKVDRLATGKLIHERFRSFDV
ECCCCHHHHHHHHHHHCCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCH
LPVVTGFIASAPDGSVTNLGRGGSDFTATILGAALHAEEVWIWTDVDGFYSADPKRVPDA
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCC
RVIPEISYAEAMELSHAGAKVLHPLAVQPVMKAGIPLRIKNSFNPEKPGTRIGIEAAGAE
CCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEEEECCCC
ALPGTVTGLTSINHVVLLSLSGSGMAGVPGTASRLFTCLARHSINIIFISQASSEQSISL
CCCCCHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEE
AIAPGQASMAKKVLEEEFAREIEERRIDPVSVRRNLAMVAVVGNKMLGHPGVSAQLFETL
EECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEEECCHHCCCCCCHHHHHHHH
GKNGVNVIAVAQGANEMNISLVIDSADENKALNCVHESFFLSMRKVHVFIVGTGTIAKSL
CCCCCEEEEEECCCCCEEEEEEEECCCCCCHHHHHHHHHHHEEEEEEEEEEECHHHHHHH
ISQIRRHRATLQKELGLDVVVAGLANTRSMCIEPAGIDLEHWHDSLKPRESHEGIGQYIR
HHHHHHHHHHHHHHCCCCEEEECCCCCCHHEECCCCCCHHHHHHCCCCCHHHHHHHHHHH
LIQERNLHNTIVVDCTASRQVAECYPALLRANISVATANKLGMAGPWDLYRKIMDALRSS
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCC
NAKFLYETNVGAGLPVINTLNDLKNSGDKIVCIEGVLSGTLSFIFNELRKGGRFSEIVRK
CCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHH
AKESGYTEPDPRDDLSGADFARKLLILGRELGYQLEYADVECQSLVPEPLRGEMSPAEFL
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCHHHCCCCCCCCCCCHHHHH
DQLSSIDDWYVDEMASAASEGMTIAYTGELRDGKAKVGLKRVPLESPVAGLNGSENLVVF
HHHHCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHCCEEECCCCCCCCCCCCCCCEEEE
TTERYLKTPLVVKGPGAGGEVTAGGVFADILRIASYLV
EEHHHHCCCCEEECCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7003595; 1630901; 7610040; 9278503; 6277952; 2410621; 387092; 6298218; 390305 [H]