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The map label for this gene is dapF

Identifier: 21674831

GI number: 21674831

Start: 1918261

End: 1919034

Strand: Direct

Name: dapF

Synonym: CT2021

Alternate gene names: 21674831

Gene position: 1918261-1919034 (Clockwise)

Preceding gene: 21674827

Following gene: 21674832

Centisome position: 89.02

GC content: 56.2

Gene sequence:

>774_bases
ATGTCGGGAGCTGGCAACGACTTCATCGTCATCGACAACCGTCAGGGATTGTTTAACCTGACCCATGAGCAGGTGCGCGC
CATGTGCACCCGCAGAACCGGCATCGGAGCTGACGGATTGATCCTTCTCGAAACTTCAGAAACCGCCGACTTCAGGATGA
ACTACCATAATGCAGATGGCTTTCCGGGCACAATGTGCGGCAACGGCGGTCGATGCGCTGTCTGGTTTGCCCACCTCATC
GGCATCCGGCCAACAGGGAAACACTACCGTTTCGAAGCCGGCCCGAGCACATACGAAGCTGAAGTGACCGGCGAGGAGTC
GGTCAGACTGCACATGCTTCCACCATCGGATTTCAGGGATGGATTGCAAGCCGGTGCTTGGAACTGCCATTTCGTCGATA
CCGGTTCGCCGCACGCCATCGCTTACGTCAACAACCTTGACCAGCTCGATGTATTGACCGAAGGCGGAAACATCCGCCAC
AACAAGGAATTGTTCCCTGATGGCACCAACGTCAACTTCCTTGAAATCACCGCTCCGGATGCGCTCAGCATCAGAACGTT
CGAGCGAGGCGTCGAGGATGAAACCCTCGCCTGCGGCACCGGCACCGTAGCCGCAGCCTTGATGAGCTTCCGGCTGGGCA
AGGTTACGTCGTCACTTGTCAGGGTAAAGGTCAAAAGCGGCGAAACACTGATGGTGGGATTCAATGAAATGATGGACGAG
ATATACCTTGAAGGCCCAGCCCGAGCAGTTTACCGGGGAACCATCACGCTGTAA

Upstream 100 bases:

>100_bases
ATCAAACAACTTCATTTTCCATGTTAATATCGGGTAAAACTAATATTTTACTGAAAACTGACCTTCAAAAAAAAGAGACA
TGCACATCGATTTCACCAAA

Downstream 100 bases:

>100_bases
GCCCGTCTTACAGTTAGTTTGTCGGCGGCGGGATAGGCTGTCTTGCCTGAACGTACAGACTCCGTCAACCATGCATGTGG
CAAACTTTTATCATGTGGCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSGAGNDFIVIDNRQGLFNLTHEQVRAMCTRRTGIGADGLILLETSETADFRMNYHNADGFPGTMCGNGGRCAVWFAHLI
GIRPTGKHYRFEAGPSTYEAEVTGEESVRLHMLPPSDFRDGLQAGAWNCHFVDTGSPHAIAYVNNLDQLDVLTEGGNIRH
NKELFPDGTNVNFLEITAPDALSIRTFERGVEDETLACGTGTVAAALMSFRLGKVTSSLVRVKVKSGETLMVGFNEMMDE
IYLEGPARAVYRGTITL

Sequences:

>Translated_257_residues
MSGAGNDFIVIDNRQGLFNLTHEQVRAMCTRRTGIGADGLILLETSETADFRMNYHNADGFPGTMCGNGGRCAVWFAHLI
GIRPTGKHYRFEAGPSTYEAEVTGEESVRLHMLPPSDFRDGLQAGAWNCHFVDTGSPHAIAYVNNLDQLDVLTEGGNIRH
NKELFPDGTNVNFLEITAPDALSIRTFERGVEDETLACGTGTVAAALMSFRLGKVTSSLVRVKVKSGETLMVGFNEMMDE
IYLEGPARAVYRGTITL
>Mature_256_residues
SGAGNDFIVIDNRQGLFNLTHEQVRAMCTRRTGIGADGLILLETSETADFRMNYHNADGFPGTMCGNGGRCAVWFAHLIG
IRPTGKHYRFEAGPSTYEAEVTGEESVRLHMLPPSDFRDGLQAGAWNCHFVDTGSPHAIAYVNNLDQLDVLTEGGNIRHN
KELFPDGTNVNFLEITAPDALSIRTFERGVEDETLACGTGTVAAALMSFRLGKVTSSLVRVKVKSGETLMVGFNEMMDEI
YLEGPARAVYRGTITL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=29.9270072992701, Blast_Score=107, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_CHLTE (Q8KAX9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662896.1
- ProteinModelPortal:   Q8KAX9
- GeneID:   1007540
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT2021
- NMPDR:   fig|194439.1.peg.1990
- TIGR:   CT2021
- HOGENOM:   HBG399442
- OMA:   SGNDFVI
- ProtClustDB:   PRK00450
- BioCyc:   CTEP194439:CT_2021-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 28053; Mature: 27922

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 66-66 ACT_SITE 198-198

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGAGNDFIVIDNRQGLFNLTHEQVRAMCTRRTGIGADGLILLETSETADFRMNYHNADG
CCCCCCCEEEEECCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCC
FPGTMCGNGGRCAVWFAHLIGIRPTGKHYRFEAGPSTYEAEVTGEESVRLHMLPPSDFRD
CCCCCCCCCCCHHEEEEHHHCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEECCHHHHH
GLQAGAWNCHFVDTGSPHAIAYVNNLDQLDVLTEGGNIRHNKELFPDGTNVNFLEITAPD
HHHHCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCC
ALSIRTFERGVEDETLACGTGTVAAALMSFRLGKVTSSLVRVKVKSGETLMVGFNEMMDE
CEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEHHHHHHH
IYLEGPARAVYRGTITL
HEECCCCCEEEEEEEEC
>Mature Secondary Structure 
SGAGNDFIVIDNRQGLFNLTHEQVRAMCTRRTGIGADGLILLETSETADFRMNYHNADG
CCCCCCEEEEECCCCCEECCHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEECCCCC
FPGTMCGNGGRCAVWFAHLIGIRPTGKHYRFEAGPSTYEAEVTGEESVRLHMLPPSDFRD
CCCCCCCCCCCHHEEEEHHHCCCCCCCCEEEECCCCEEEEEECCCCCEEEEEECCHHHHH
GLQAGAWNCHFVDTGSPHAIAYVNNLDQLDVLTEGGNIRHNKELFPDGTNVNFLEITAPD
HHHHCCEEEEEEECCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCC
ALSIRTFERGVEDETLACGTGTVAAALMSFRLGKVTSSLVRVKVKSGETLMVGFNEMMDE
CEEEHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHCEEEEEEECCCCEEEEEHHHHHHH
IYLEGPARAVYRGTITL
HEECCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901