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The map label for this gene is kefA [H]

Identifier: 21674826

GI number: 21674826

Start: 1913019

End: 1913924

Strand: Reverse

Name: kefA [H]

Synonym: CT2016

Alternate gene names: 21674826

Gene position: 1913924-1913019 (Counterclockwise)

Preceding gene: 21674828

Following gene: 21674823

Centisome position: 88.82

GC content: 51.88

Gene sequence:

>906_bases
ATGGAATCAACATCATTGCGTGATCTATCTCACGTAACCGACTGGCTTGTAAGGCCGCTCGTCGTGATCGGTCATGCTGA
AATATCGATTGTCAAAATCGTGACCATCATCCTGTCGGTTGCGCTGATCGTCGCTGCTGCGAAGTTTCTGAAGCGGATGC
TTGTCGGGCGGCTTCTGGTCTCTTCGGTGCACGATACAGGGACCCGCTCAGCTCTGGCTACCATTCTTCAGTACCTGATC
GTTTTTTTTGGTATCCTCATCGTGCTGCAATCGGCGGGGATCGATCTCAGCTCCTTAACCGTGCTGTCGGGTACTATCGG
TCTTGGTATCGGTTTCGGATTGCAGAATATCGCCGATAACTTTTTCAGCGGCCTGATCATTTTGCTGGAACGCCCGGTCA
AGGTTGGCGACCGCATTCAGGTCGGAGAGATCAACGGGGATGTTGTCAGGATAGCCATCCGCTCAACGACGCTTCTGACC
AACGACAATATCAACATCATCATTCCGAATTCGGAATTCGTTTCAAAGCAGGTCATCAACTGGAGCCACAATGACCGGAG
TCTCAGGGTGTCGGTGCCTGTCGGAGTTGCCTATGGTTCCGATCCGGAACAGGTTAAGCATGTGCTGCTTGGTGTTGCGG
AGAATCACCCCGATATTCTGACCAAGCCTTCTCCAGCGGTGCTTTTTTCCGATTTTGGCAACAGTTCGCTGGATTTTGAA
CTTCTTGTCTGGACAGAGACACGAATCCAGACGCCACGCTTTCTGCGGAGCGAACTGAACTACAGGATTTTCGATGCCTT
CAGGAAGAACGGTATCGAGATTCCCTTCCCGCAGACCGACCTGCATATCAGAAGCAGTGATATCCCGTTGTGGGAACCGC
GTGAACGGAAGAACCCAACAGGGTGA

Upstream 100 bases:

>100_bases
GGGGTTGACTTTGGTTGTAGAAAACGCAGATCGATACACAAAATGGTTTCAGTGTTTTCATTGGAACTGACATACATGCT
TTCATGCAAACCAAACGGGT

Downstream 100 bases:

>100_bases
TGTGTGAGGCTTTCTAGAGCTGTTACCGGCGAGGTTTTGGTATTAAGCGATTTGATTGCTACCATGTAAAAGCGCATAAG
TTATAGAAATCATTCATCGA

Product: putative AefA

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MESTSLRDLSHVTDWLVRPLVVIGHAEISIVKIVTIILSVALIVAAAKFLKRMLVGRLLVSSVHDTGTRSALATILQYLI
VFFGILIVLQSAGIDLSSLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGEINGDVVRIAIRSTTLLT
NDNINIIIPNSEFVSKQVINWSHNDRSLRVSVPVGVAYGSDPEQVKHVLLGVAENHPDILTKPSPAVLFSDFGNSSLDFE
LLVWTETRIQTPRFLRSELNYRIFDAFRKNGIEIPFPQTDLHIRSSDIPLWEPRERKNPTG

Sequences:

>Translated_301_residues
MESTSLRDLSHVTDWLVRPLVVIGHAEISIVKIVTIILSVALIVAAAKFLKRMLVGRLLVSSVHDTGTRSALATILQYLI
VFFGILIVLQSAGIDLSSLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGEINGDVVRIAIRSTTLLT
NDNINIIIPNSEFVSKQVINWSHNDRSLRVSVPVGVAYGSDPEQVKHVLLGVAENHPDILTKPSPAVLFSDFGNSSLDFE
LLVWTETRIQTPRFLRSELNYRIFDAFRKNGIEIPFPQTDLHIRSSDIPLWEPRERKNPTG
>Mature_301_residues
MESTSLRDLSHVTDWLVRPLVVIGHAEISIVKIVTIILSVALIVAAAKFLKRMLVGRLLVSSVHDTGTRSALATILQYLI
VFFGILIVLQSAGIDLSSLTVLSGTIGLGIGFGLQNIADNFFSGLIILLERPVKVGDRIQVGEINGDVVRIAIRSTTLLT
NDNINIIIPNSEFVSKQVINWSHNDRSLRVSVPVGVAYGSDPEQVKHVLLGVAENHPDILTKPSPAVLFSDFGNSSLDFE
LLVWTETRIQTPRFLRSELNYRIFDAFRKNGIEIPFPQTDLHIRSSDIPLWEPRERKNPTG

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=254, Percent_Identity=39.3700787401575, Blast_Score=181, Evalue=6e-47,
Organism=Escherichia coli, GI2367355, Length=220, Percent_Identity=35.9090909090909, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1789291, Length=289, Percent_Identity=30.1038062283737, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787591, Length=257, Percent_Identity=27.2373540856031, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 33260; Mature: 33260

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESTSLRDLSHVTDWLVRPLVVIGHAEISIVKIVTIILSVALIVAAAKFLKRMLVGRLLV
CCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVHDTGTRSALATILQYLIVFFGILIVLQSAGIDLSSLTVLSGTIGLGIGFGLQNIADN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FFSGLIILLERPVKVGDRIQVGEINGDVVRIAIRSTTLLTNDNINIIIPNSEFVSKQVIN
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHHC
WSHNDRSLRVSVPVGVAYGSDPEQVKHVLLGVAENHPDILTKPSPAVLFSDFGNSSLDFE
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEE
LLVWTETRIQTPRFLRSELNYRIFDAFRKNGIEIPFPQTDLHIRSSDIPLWEPRERKNPT
EEEEECCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
G
C
>Mature Secondary Structure
MESTSLRDLSHVTDWLVRPLVVIGHAEISIVKIVTIILSVALIVAAAKFLKRMLVGRLLV
CCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVHDTGTRSALATILQYLIVFFGILIVLQSAGIDLSSLTVLSGTIGLGIGFGLQNIADN
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHH
FFSGLIILLERPVKVGDRIQVGEINGDVVRIAIRSTTLLTNDNINIIIPNSEFVSKQVIN
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCEEEEECCCHHHHHHHHC
WSHNDRSLRVSVPVGVAYGSDPEQVKHVLLGVAENHPDILTKPSPAVLFSDFGNSSLDFE
CCCCCCEEEEEEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCEE
LLVWTETRIQTPRFLRSELNYRIFDAFRKNGIEIPFPQTDLHIRSSDIPLWEPRERKNPT
EEEEECCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]