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The map label for this gene is eno

Identifier: 21674774

GI number: 21674774

Start: 1859656

End: 1860939

Strand: Reverse

Name: eno

Synonym: CT1962

Alternate gene names: 21674774

Gene position: 1860939-1859656 (Counterclockwise)

Preceding gene: 21674775

Following gene: 21674773

Centisome position: 86.36

GC content: 36.76

Gene sequence:

>1284_bases
ATGAAAATTCAAAATGTTAATGCTATCGAGATTCTCGATTCGAGAGGAAACCCAACCGTTGAAGTGAATTTAAAACTTGA
AGACGGTACTATTTCTCGTGCAATGGTTCCAAGTGGAGCATCAACGGGAGAAAGAGAAGCAACAGAACTTAGAGATGGCG
ACAAAAAGCGCTATGGCGGTAAAGGTGTTTTAAAAGCTGTCGAGAATGTTAATTCAGCCATTGCAAAAGCCATTGAGAAT
AAACACTTTACCAATCAACGAGAATTGGACTATTTTCTTATTGAACTTGACGAAACTAATAACAAATCTAAACTAGGGGC
TAATGCGATACTTGGTGTTTCAATGGCTTTTGCCCGTGCAAAAGCGCAAAGTTCACGTACTCCGCTATATCAATATTTAG
GCGGCAGTAATGCTCACATTATGCCTGTTCCCTGTATGAATGTCATCAATGGAGGTAAACATGCAGACAATACAATAGAC
TTTCAGGAATTTATGATTGCTCCACATAACGCTCCATCGTTTAGAGAATCAATCCGTATGGGCGAAGAAGTTTTTCACGC
CTTGAAAGCTGTATTAAAGTTAAAAGGATTAAGCACTGGCGTTGGAGATGAAGGCGGTTTTGCTCCAGACTTAAAATCCA
ATGAACAAGCCGTTGAAATGATATTAGAAGGGATTACTAAAGCTGGTTATAAGCCAAGTGTAGATGTTAGTATTTGTCTT
GACCCGGCTTCAAGTGAAATGTGGGAAAATGGGAAATACAAATTCTTTAAAAGTACTCAGAAATTGGTTTCCAGTGATGA
AATGGTGAAATTGTGGGAAAGCTGGGTAAATCAATACCCAATTGTATTACTAGAAGATGGAATGGCTGAAAATGACTGGG
AAGGTTGGAAAAATCTTACAGATGTAATCGGAAACAAAATCGAGATTGTTGGCGATGATTTGTTCTGTACTAATAAATCA
ATTTTATTAAATGGTATAAATAAAGGCGTTGCAAATTCTATCCTGATAAAATTGAATCAAATAGGTACTGTGACAGAGAC
TTTAGAAACGATTGAACTTGCATATAAAAATTCATATAATTGTTTTGTTTCCCATCGGAGTGGAGAAACGGTTGATAGTT
TTATTGCCGATTTGACAGTAGGAATTAATGCTGGACATCTTAAGAGTGGTAGTGGTTGTCGAGGAGAAAGAATAGAAAAA
TTCAATCAACTTATGCGGATTGAAAATGAATTGGGTAAATCAGCTCAATTTGCAGGATTGAAAGCCTTTAAAAATGCAAA
ATAA

Upstream 100 bases:

>100_bases
AACATACGCATCATCATCTTAATTTTTGTAAATTGATTGAACATCTTAATTTTTGTAAATTGATTGAAAATTTTTTTTAA
AAAAATGGAGGAAATAAGTT

Downstream 100 bases:

>100_bases
AAACTGAAATTAATAACGCCAAGCACACAACACACAATATAAAACAGTTAGGGTTCAGTGGTTTGCGATGGTTTGGTTCT
CGCGCCAACTTTCTTATCGG

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase 1; 2-phosphoglycerate dehydratase 1

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKIQNVNAIEILDSRGNPTVEVNLKLEDGTISRAMVPSGASTGEREATELRDGDKKRYGGKGVLKAVENVNSAIAKAIEN
KHFTNQRELDYFLIELDETNNKSKLGANAILGVSMAFARAKAQSSRTPLYQYLGGSNAHIMPVPCMNVINGGKHADNTID
FQEFMIAPHNAPSFRESIRMGEEVFHALKAVLKLKGLSTGVGDEGGFAPDLKSNEQAVEMILEGITKAGYKPSVDVSICL
DPASSEMWENGKYKFFKSTQKLVSSDEMVKLWESWVNQYPIVLLEDGMAENDWEGWKNLTDVIGNKIEIVGDDLFCTNKS
ILLNGINKGVANSILIKLNQIGTVTETLETIELAYKNSYNCFVSHRSGETVDSFIADLTVGINAGHLKSGSGCRGERIEK
FNQLMRIENELGKSAQFAGLKAFKNAK

Sequences:

>Translated_427_residues
MKIQNVNAIEILDSRGNPTVEVNLKLEDGTISRAMVPSGASTGEREATELRDGDKKRYGGKGVLKAVENVNSAIAKAIEN
KHFTNQRELDYFLIELDETNNKSKLGANAILGVSMAFARAKAQSSRTPLYQYLGGSNAHIMPVPCMNVINGGKHADNTID
FQEFMIAPHNAPSFRESIRMGEEVFHALKAVLKLKGLSTGVGDEGGFAPDLKSNEQAVEMILEGITKAGYKPSVDVSICL
DPASSEMWENGKYKFFKSTQKLVSSDEMVKLWESWVNQYPIVLLEDGMAENDWEGWKNLTDVIGNKIEIVGDDLFCTNKS
ILLNGINKGVANSILIKLNQIGTVTETLETIELAYKNSYNCFVSHRSGETVDSFIADLTVGINAGHLKSGSGCRGERIEK
FNQLMRIENELGKSAQFAGLKAFKNAK
>Mature_427_residues
MKIQNVNAIEILDSRGNPTVEVNLKLEDGTISRAMVPSGASTGEREATELRDGDKKRYGGKGVLKAVENVNSAIAKAIEN
KHFTNQRELDYFLIELDETNNKSKLGANAILGVSMAFARAKAQSSRTPLYQYLGGSNAHIMPVPCMNVINGGKHADNTID
FQEFMIAPHNAPSFRESIRMGEEVFHALKAVLKLKGLSTGVGDEGGFAPDLKSNEQAVEMILEGITKAGYKPSVDVSICL
DPASSEMWENGKYKFFKSTQKLVSSDEMVKLWESWVNQYPIVLLEDGMAENDWEGWKNLTDVIGNKIEIVGDDLFCTNKS
ILLNGINKGVANSILIKLNQIGTVTETLETIELAYKNSYNCFVSHRSGETVDSFIADLTVGINAGHLKSGSGCRGERIEK
FNQLMRIENELGKSAQFAGLKAFKNAK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=50, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI301897477, Length=438, Percent_Identity=50, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=438, Percent_Identity=50, Blast_Score=403, Evalue=1e-112,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=49.5412844036697, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI301897479, Length=436, Percent_Identity=45.1834862385321, Blast_Score=345, Evalue=7e-95,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=23.4421364985163, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=23.4421364985163, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=23.4421364985163, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1789141, Length=427, Percent_Identity=52.4590163934426, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI71995829, Length=439, Percent_Identity=48.5193621867882, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17536383, Length=439, Percent_Identity=48.5193621867882, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32563855, Length=197, Percent_Identity=42.6395939086294, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=47.3441108545035, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=47.1131639722864, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=47.1131639722864, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=46.8822170900693, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=47.3441108545035, Blast_Score=352, Evalue=5e-98,
Organism=Drosophila melanogaster, GI24580918, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580916, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580920, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24580914, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI281360527, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17137654, Length=439, Percent_Identity=48.2915717539863, Blast_Score=383, Evalue=1e-106,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO1_CHLTE (Q8KB35)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662839.1
- ProteinModelPortal:   Q8KB35
- SMR:   Q8KB35
- GeneID:   1007989
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1962
- NMPDR:   fig|194439.1.peg.1933
- TIGR:   CT1962
- HOGENOM:   HBG726599
- OMA:   CMNVING
- ProtClustDB:   PRK00077
- BioCyc:   CTEP194439:CT_1962-MONOMER
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46839; Mature: 46839

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00164 ENOLASE; PS00290 IG_MHC

Important sites: ACT_SITE 204-204 ACT_SITE 337-337 BINDING 154-154 BINDING 163-163 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIQNVNAIEILDSRGNPTVEVNLKLEDGTISRAMVPSGASTGEREATELRDGDKKRYGG
CCCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHHHHHCCCCHHHCCC
KGVLKAVENVNSAIAKAIENKHFTNQRELDYFLIELDETNNKSKLGANAILGVSMAFARA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KAQSSRTPLYQYLGGSNAHIMPVPCMNVINGGKHADNTIDFQEFMIAPHNAPSFRESIRM
HHHCCCCCHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHEECCCCCHHHHHHHHH
GEEVFHALKAVLKLKGLSTGVGDEGGFAPDLKSNEQAVEMILEGITKAGYKPSVDVSICL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
DPASSEMWENGKYKFFKSTQKLVSSDEMVKLWESWVNQYPIVLLEDGMAENDWEGWKNLT
CCCCHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
DVIGNKIEIVGDDLFCTNKSILLNGINKGVANSILIKLNQIGTVTETLETIELAYKNSYN
HHCCCEEEEEECCEEECCCHHEEECCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCE
CFVSHRSGETVDSFIADLTVGINAGHLKSGSGCRGERIEKFNQLMRIENELGKSAQFAGL
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KAFKNAK
HHHCCCC
>Mature Secondary Structure
MKIQNVNAIEILDSRGNPTVEVNLKLEDGTISRAMVPSGASTGEREATELRDGDKKRYGG
CCCCCCCEEEEEECCCCCEEEEEEEEECCCCCEEECCCCCCCCCHHHHHHCCCCHHHCCC
KGVLKAVENVNSAIAKAIENKHFTNQRELDYFLIELDETNNKSKLGANAILGVSMAFARA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHH
KAQSSRTPLYQYLGGSNAHIMPVPCMNVINGGKHADNTIDFQEFMIAPHNAPSFRESIRM
HHHCCCCCHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCHHHHEECCCCCHHHHHHHHH
GEEVFHALKAVLKLKGLSTGVGDEGGFAPDLKSNEQAVEMILEGITKAGYKPSVDVSICL
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
DPASSEMWENGKYKFFKSTQKLVSSDEMVKLWESWVNQYPIVLLEDGMAENDWEGWKNLT
CCCCHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHH
DVIGNKIEIVGDDLFCTNKSILLNGINKGVANSILIKLNQIGTVTETLETIELAYKNSYN
HHCCCEEEEEECCEEECCCHHEEECCCCCHHHHEEEEECCCCCHHHHHHHHHHHHCCCCE
CFVSHRSGETVDSFIADLTVGINAGHLKSGSGCRGERIEKFNQLMRIENELGKSAQFAGL
EEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHH
KAFKNAK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901