Click here to switch to the map view.

The map label for this gene is lpd-2 [H]

Identifier: 21674773

GI number: 21674773

Start: 1858074

End: 1859477

Strand: Reverse

Name: lpd-2 [H]

Synonym: CT1961

Alternate gene names: 21674773

Gene position: 1859477-1858074 (Counterclockwise)

Preceding gene: 21674774

Following gene: 21674766

Centisome position: 86.29

GC content: 41.95

Gene sequence:

>1404_bases
ATGAGCACTAAATTCGATGTTATCATAATTGGAGGTGGCCCTGGTGGAACCCCGGCCGCTATGCAGTTAGCTTCTCAAGG
CAAAACCGTTTTACTTGTTGAAGAATCTGGAAAACTTGGAGGTGCATGTTTGTTTGTCGGTTGCATTCCGTCCAAGATTA
TCCGGCATTGGGCGGATGAGTATGCTGTTAAGCTCAAATACTCTGCTCAGGAGGCTCTGTCACCAGAGGACAGAGAAGCG
GCGTGGAATGAGATTATGCGTAAAATGCAGACCATTCTCAGTCAACGATCTGGTGCAGCGATGCAAATGTTAAAGCATTT
GTCAAATCTAAGGTTTGTTGCTGGTCATGCCAAATTTGTTTCTAATAATGAATTGGTTATCAACGAAAAAGATACCGGAC
GAAAAGAAAAATATACCTTCAATAAGGCCATTATAGCCACGGGGTCTCACTCTTTCATACCTCCCTTCAAGGGCAATGGT
GTTCAAGATGTGTTGACAAGCGAAGTACTTTTTTCGCAAGATAAACTTCCCGAATCACTACTTATTATCGGAGGGGGGCC
AATCGGTATTGAATTGGCTCAAATGCTTACCAAGTTAGGCACGAAATGCACAATCATTGAACTGCTTGATAGCATTCTGT
ATGGCGTTGTTGAGACAGAGTTTGTCTCAATCATTAGCAATCAGCTTTCTAGCTTAGGAGTCAACATTTACACCTCATCC
CAAGTGCAGGAAATCAATAAGTCCGATGGGCATTTTGATGTGACATTCACAGATGCAAATGGCTCTGAGCACAAAGAAAA
TTTCGAGGATGTGCTTGTAGTTACAGGAAAAGTACCGAATATTGAATCTCTCAATTTAGATTCGACTGATATAAAATACG
ACCGAAAAGGGATCATCGTTGATGAATATCTTGAAACTTCCGTGAAGGGTATTTATGCTACAGGCGATGTCACTCATGGG
CCGAAATTTGCTCATACTGCCACCTATGAAGCTCATATAGCATCAGCAAATATTTCAGCGGGAAATAATCAGAAAGTCGA
TTTTTCCAAAAACACCTGGGTACTATTCTCTGAGCCGGAGATTGTTGCAGCAGGGTTTACAGAGGCTCAGGCCGTTCAAG
AAGGGTATGATATTATTACCGGTGTGTATGATTACAAAATTGACGCAGCTGCTCAAGTTATGAATTCGCCTTTTGGCTAT
CTGAAGTATGTGGTCAACAAAAAAAACTCGGAAATTATAGGCGTCCACATTTGCATGAATAACGCATCATCGTTGGCAGG
AGAAGCCTCATTGATTATAGCAAATCGATTAATTCTCAAAAATGTCGCCGAAACAATACATCCGCACCCTACTCTGACGG
AAGCATTTGGCATATTGGCCCAAAAGATGCTCAGTAAAAGCTAA

Upstream 100 bases:

>100_bases
CTCGCGCCAACTTTCTTATCGGTGGACAAGAACGTAGCCTAAAAAACCAGCCGTTTCATATTGTCAAACGTTAGCTTTTA
CTATTCAAAGGAGACTTATT

Downstream 100 bases:

>100_bases
CAAGGCGCTCCACCTGACCGCTATTCCGCTACGCTCCATAACGACAGGTGAGCTTAAGCGTTGCGCTGCCAGTCGTCCGC
AGGAAGGTTCAGCGCCCGCT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSTKFDVIIIGGGPGGTPAAMQLASQGKTVLLVEESGKLGGACLFVGCIPSKIIRHWADEYAVKLKYSAQEALSPEDREA
AWNEIMRKMQTILSQRSGAAMQMLKHLSNLRFVAGHAKFVSNNELVINEKDTGRKEKYTFNKAIIATGSHSFIPPFKGNG
VQDVLTSEVLFSQDKLPESLLIIGGGPIGIELAQMLTKLGTKCTIIELLDSILYGVVETEFVSIISNQLSSLGVNIYTSS
QVQEINKSDGHFDVTFTDANGSEHKENFEDVLVVTGKVPNIESLNLDSTDIKYDRKGIIVDEYLETSVKGIYATGDVTHG
PKFAHTATYEAHIASANISAGNNQKVDFSKNTWVLFSEPEIVAAGFTEAQAVQEGYDIITGVYDYKIDAAAQVMNSPFGY
LKYVVNKKNSEIIGVHICMNNASSLAGEASLIIANRLILKNVAETIHPHPTLTEAFGILAQKMLSKS

Sequences:

>Translated_467_residues
MSTKFDVIIIGGGPGGTPAAMQLASQGKTVLLVEESGKLGGACLFVGCIPSKIIRHWADEYAVKLKYSAQEALSPEDREA
AWNEIMRKMQTILSQRSGAAMQMLKHLSNLRFVAGHAKFVSNNELVINEKDTGRKEKYTFNKAIIATGSHSFIPPFKGNG
VQDVLTSEVLFSQDKLPESLLIIGGGPIGIELAQMLTKLGTKCTIIELLDSILYGVVETEFVSIISNQLSSLGVNIYTSS
QVQEINKSDGHFDVTFTDANGSEHKENFEDVLVVTGKVPNIESLNLDSTDIKYDRKGIIVDEYLETSVKGIYATGDVTHG
PKFAHTATYEAHIASANISAGNNQKVDFSKNTWVLFSEPEIVAAGFTEAQAVQEGYDIITGVYDYKIDAAAQVMNSPFGY
LKYVVNKKNSEIIGVHICMNNASSLAGEASLIIANRLILKNVAETIHPHPTLTEAFGILAQKMLSKS
>Mature_466_residues
STKFDVIIIGGGPGGTPAAMQLASQGKTVLLVEESGKLGGACLFVGCIPSKIIRHWADEYAVKLKYSAQEALSPEDREAA
WNEIMRKMQTILSQRSGAAMQMLKHLSNLRFVAGHAKFVSNNELVINEKDTGRKEKYTFNKAIIATGSHSFIPPFKGNGV
QDVLTSEVLFSQDKLPESLLIIGGGPIGIELAQMLTKLGTKCTIIELLDSILYGVVETEFVSIISNQLSSLGVNIYTSSQ
VQEINKSDGHFDVTFTDANGSEHKENFEDVLVVTGKVPNIESLNLDSTDIKYDRKGIIVDEYLETSVKGIYATGDVTHGP
KFAHTATYEAHIASANISAGNNQKVDFSKNTWVLFSEPEIVAAGFTEAQAVQEGYDIITGVYDYKIDAAAQVMNSPFGYL
KYVVNKKNSEIIGVHICMNNASSLAGEASLIIANRLILKNVAETIHPHPTLTEAFGILAQKMLSKS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=29.7592997811816, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI50301238, Length=436, Percent_Identity=28.8990825688073, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI22035672, Length=456, Percent_Identity=25.219298245614, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI291045266, Length=469, Percent_Identity=23.0277185501066, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI33519430, Length=466, Percent_Identity=22.1030042918455, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI33519428, Length=466, Percent_Identity=22.1030042918455, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI33519426, Length=466, Percent_Identity=22.1030042918455, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=22.1030042918455, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI148277071, Length=466, Percent_Identity=22.1030042918455, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI291045268, Length=465, Percent_Identity=22.5806451612903, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=29.7413793103448, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=27.5488069414317, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87082354, Length=473, Percent_Identity=26.0042283298097, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=25.8928571428571, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1789065, Length=290, Percent_Identity=27.9310344827586, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=457, Percent_Identity=29.9781181619256, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI17557007, Length=475, Percent_Identity=25.8947368421053, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=355, Percent_Identity=27.6056338028169, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71983429, Length=355, Percent_Identity=27.6056338028169, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=495, Percent_Identity=24.6464646464646, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=465, Percent_Identity=28.6021505376344, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=472, Percent_Identity=27.3305084745763, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325166, Length=470, Percent_Identity=25.7446808510638, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21358499, Length=461, Percent_Identity=30.1518438177874, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24640553, Length=480, Percent_Identity=23.9583333333333, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=486, Percent_Identity=24.2798353909465, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640549, Length=480, Percent_Identity=23.9583333333333, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17737741, Length=476, Percent_Identity=23.9495798319328, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50687; Mature: 50556

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKFDVIIIGGGPGGTPAAMQLASQGKTVLLVEESGKLGGACLFVGCIPSKIIRHWADE
CCCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHH
YAVKLKYSAQEALSPEDREAAWNEIMRKMQTILSQRSGAAMQMLKHLSNLRFVAGHAKFV
HEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCEEEE
SNNELVINEKDTGRKEKYTFNKAIIATGSHSFIPPFKGNGVQDVLTSEVLFSQDKLPESL
CCCEEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
LIIGGGPIGIELAQMLTKLGTKCTIIELLDSILYGVVETEFVSIISNQLSSLGVNIYTSS
EEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECH
QVQEINKSDGHFDVTFTDANGSEHKENFEDVLVVTGKVPNIESLNLDSTDIKYDRKGIIV
HHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
DEYLETSVKGIYATGDVTHGPKFAHTATYEAHIASANISAGNNQKVDFSKNTWVLFSEPE
HHHHHHCCCEEEEECCCCCCCCCCEECEEEEEEEECCCCCCCCCEEEECCCEEEEEECCC
IVAAGFTEAQAVQEGYDIITGVYDYKIDAAAQVMNSPFGYLKYVVNKKNSEIIGVHICMN
EEEECCHHHHHHHHCHHHHEEEEEEEHHHHHHHHHCCHHHHHHEECCCCCEEEEEEEEEC
NASSLAGEASLIIANRLILKNVAETIHPHPTLTEAFGILAQKMLSKS
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STKFDVIIIGGGPGGTPAAMQLASQGKTVLLVEESGKLGGACLFVGCIPSKIIRHWADE
CCEEEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHH
YAVKLKYSAQEALSPEDREAAWNEIMRKMQTILSQRSGAAMQMLKHLSNLRFVAGHAKFV
HEEEEEECHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEECCEEEE
SNNELVINEKDTGRKEKYTFNKAIIATGSHSFIPPFKGNGVQDVLTSEVLFSQDKLPESL
CCCEEEEECCCCCCCCEEEECEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEE
LIIGGGPIGIELAQMLTKLGTKCTIIELLDSILYGVVETEFVSIISNQLSSLGVNIYTSS
EEECCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECH
QVQEINKSDGHFDVTFTDANGSEHKENFEDVLVVTGKVPNIESLNLDSTDIKYDRKGIIV
HHHHHCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCH
DEYLETSVKGIYATGDVTHGPKFAHTATYEAHIASANISAGNNQKVDFSKNTWVLFSEPE
HHHHHHCCCEEEEECCCCCCCCCCEECEEEEEEEECCCCCCCCCEEEECCCEEEEEECCC
IVAAGFTEAQAVQEGYDIITGVYDYKIDAAAQVMNSPFGYLKYVVNKKNSEIIGVHICMN
EEEECCHHHHHHHHCHHHHEEEEEEEHHHHHHHHHCCHHHHHHEECCCCCEEEEEEEEEC
NASSLAGEASLIIANRLILKNVAETIHPHPTLTEAFGILAQKMLSKS
CCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2200674; 2253629; 6896188; 8805537 [H]