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The map label for this gene is galE [C]

Identifier: 21674761

GI number: 21674761

Start: 1840582

End: 1841589

Strand: Reverse

Name: galE [C]

Synonym: CT1949

Alternate gene names: 21674761

Gene position: 1841589-1840582 (Counterclockwise)

Preceding gene: 21674766

Following gene: 21674757

Centisome position: 85.46

GC content: 61.31

Gene sequence:

>1008_bases
ATGTTTATGGACGGAGTGATTCTGGTTACCGGTTCGACCGGTTTTATCGGCTCGAGAATGGTCGATGCCCTCGTTGGGCA
GGGCCGGCGGGTGCGGGTACTGCTCAGGCCTGAAAGCCGCAGCACATTGTCGGCGGGATATCGCGAAGGCGTTGAAGAGG
TTTGCGCCGCTTACGGCGATCCGGAAGCCCTCGGGAGAGCGGTTTCTGGCGTGGCATCGATCATCCACCTTGCCGGCGTG
ACCAAAGCGGTCGATGAAGCAGGATTTGCCGAGGGAAACGTGAGGCCGGTTGAAAATCTGCTCGAAGCGGTGAAACGCCA
CAATCCGGGGCTTGGCCGTTTTTTGCTGGTTTCGTCGCTTGCCGCCATGGGCCCGGCATCGTCACCGTCTCCGGGCGTCA
TGGAATCTGACCGGCCTCGCCCGGTGAGCGCTTACGGGCGCAGCAAGCTGCTCGGCGAAGCGGTTGCGCGGCGACACGCC
GGAAGCGTTCCGCTCACCATTGTTCGGCCACCTGCCGTGTACGGTCCTGGCGATCGCGATATTCTCGAAGTTTTCACCAT
GATGAAGAACGGATATCTGCTCTCGGCGGGTCCCGGGCGACGCCAGCGTTTCAGCATGATCCATGTCGATGAGCTGATCC
GCGGAATTTTGCTCGCACTTGACTCGGAAAACGCCGCCGGTCAGGACTATTTTATCACCTCGCCTCGCGGCTACGCCTGG
GACGAGGTGATCGCGGCGGCCCGTCCGGTGCTCGGTTTCAGGCGACTGCTTCGCCTCAACCTGCCGAAGCCGCTGGTGTT
CGGTTTGGGCGCGGTGCTCGGCGGCGTTGCTAAGTTGACCGGCTGCCCAGCTCTGATCAACAAGGACAAAGCCAATGAAC
TGGTGCAGGATTTCTGGGTATGCAGCCCGGAAAAAGCGGAGCGGGAACTCGGGTTTACCGCGAGCATTCCACTGGAAACG
GGGGTTCCCGAAACGTTGGTGTGGTACCGGCAGCAGGGGTGGCTGTAG

Upstream 100 bases:

>100_bases
TTTTGATAAACAGGGCTGTGTTTTTTGTCCGCTCGTCAGGGCGTTGTCTGTTGGTGTGCACGGGCAGTAAAGGACGGTAA
TTTCTGGATAAAAGAGGATA

Downstream 100 bases:

>100_bases
AGAACAGCTGTTCACTCAAGCCGCTGCTGTTTTCCTCAATTGAAGAGTTGCTGCATCAGTTTGAGCAGGGTGAATCTGTC
GTCGGGCGAGGGAATAATGC

Product: NAD-dependent epimerase/dehydratase family protein/3-beta hydroxysteroid dehydrogenase/isomerase family protein

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MFMDGVILVTGSTGFIGSRMVDALVGQGRRVRVLLRPESRSTLSAGYREGVEEVCAAYGDPEALGRAVSGVASIIHLAGV
TKAVDEAGFAEGNVRPVENLLEAVKRHNPGLGRFLLVSSLAAMGPASSPSPGVMESDRPRPVSAYGRSKLLGEAVARRHA
GSVPLTIVRPPAVYGPGDRDILEVFTMMKNGYLLSAGPGRRQRFSMIHVDELIRGILLALDSENAAGQDYFITSPRGYAW
DEVIAAARPVLGFRRLLRLNLPKPLVFGLGAVLGGVAKLTGCPALINKDKANELVQDFWVCSPEKAERELGFTASIPLET
GVPETLVWYRQQGWL

Sequences:

>Translated_335_residues
MFMDGVILVTGSTGFIGSRMVDALVGQGRRVRVLLRPESRSTLSAGYREGVEEVCAAYGDPEALGRAVSGVASIIHLAGV
TKAVDEAGFAEGNVRPVENLLEAVKRHNPGLGRFLLVSSLAAMGPASSPSPGVMESDRPRPVSAYGRSKLLGEAVARRHA
GSVPLTIVRPPAVYGPGDRDILEVFTMMKNGYLLSAGPGRRQRFSMIHVDELIRGILLALDSENAAGQDYFITSPRGYAW
DEVIAAARPVLGFRRLLRLNLPKPLVFGLGAVLGGVAKLTGCPALINKDKANELVQDFWVCSPEKAERELGFTASIPLET
GVPETLVWYRQQGWL
>Mature_335_residues
MFMDGVILVTGSTGFIGSRMVDALVGQGRRVRVLLRPESRSTLSAGYREGVEEVCAAYGDPEALGRAVSGVASIIHLAGV
TKAVDEAGFAEGNVRPVENLLEAVKRHNPGLGRFLLVSSLAAMGPASSPSPGVMESDRPRPVSAYGRSKLLGEAVARRHA
GSVPLTIVRPPAVYGPGDRDILEVFTMMKNGYLLSAGPGRRQRFSMIHVDELIRGILLALDSENAAGQDYFITSPRGYAW
DEVIAAARPVLGFRRLLRLNLPKPLVFGLGAVLGGVAKLTGCPALINKDKANELVQDFWVCSPEKAERELGFTASIPLET
GVPETLVWYRQQGWL

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0702

COG function: function code MG; Predicted nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35959; Mature: 35959

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFMDGVILVTGSTGFIGSRMVDALVGQGRRVRVLLRPESRSTLSAGYREGVEEVCAAYGD
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PEALGRAVSGVASIIHLAGVTKAVDEAGFAEGNVRPVENLLEAVKRHNPGLGRFLLVSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
AAMGPASSPSPGVMESDRPRPVSAYGRSKLLGEAVARRHAGSVPLTIVRPPAVYGPGDRD
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHH
ILEVFTMMKNGYLLSAGPGRRQRFSMIHVDELIRGILLALDSENAAGQDYFITSPRGYAW
HHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCH
DEVIAAARPVLGFRRLLRLNLPKPLVFGLGAVLGGVAKLTGCPALINKDKANELVQDFWV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHC
CSPEKAERELGFTASIPLETGVPETLVWYRQQGWL
CCCHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MFMDGVILVTGSTGFIGSRMVDALVGQGRRVRVLLRPESRSTLSAGYREGVEEVCAAYGD
CCCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCC
PEALGRAVSGVASIIHLAGVTKAVDEAGFAEGNVRPVENLLEAVKRHNPGLGRFLLVSSL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHH
AAMGPASSPSPGVMESDRPRPVSAYGRSKLLGEAVARRHAGSVPLTIVRPPAVYGPGDRD
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCHH
ILEVFTMMKNGYLLSAGPGRRQRFSMIHVDELIRGILLALDSENAAGQDYFITSPRGYAW
HHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCH
DEVIAAARPVLGFRRLLRLNLPKPLVFGLGAVLGGVAKLTGCPALINKDKANELVQDFWV
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHC
CSPEKAERELGFTASIPLETGVPETLVWYRQQGWL
CCCHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]