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The map label for this gene is flp [H]

Identifier: 21674535

GI number: 21674535

Start: 1630412

End: 1631089

Strand: Reverse

Name: flp [H]

Synonym: CT1719

Alternate gene names: 21674535

Gene position: 1631089-1630412 (Counterclockwise)

Preceding gene: 21674536

Following gene: 21674534

Centisome position: 75.69

GC content: 52.8

Gene sequence:

>678_bases
ATGTCGCTTGACAATAACAAGGGAAAAGCCTCGCACGCTACTCGCCAGATTCTTGAGAAATATCTGACACTACGCTCCTT
TCGCAAAGGGCAGCTTCTCTGGAGTGAGGGGGATACCGACGGCCTGCTGGTTTTTCTTAAGTCCGGGCGAGTCAAAATAT
ACCGTTTGCTGCCTATGGGAAAGGCAATAACCCTCTATATCTTCGGCAAGGGCTCTGTGTTCGGATTCATGCCGTTTTTC
GACGACGCCCCTTACCCTGCGTACGCCCAGGCGCTGGATGACTGCGAGGCGGATGTCATTTCCCGCTCGGGACTACGCCA
GGCAGTGCATCAAGATCCGGAAGTGGCAATCGTCCTCATGAAACAGCTGGCTCAACGTCTTCGTGATGCCTTTGATACGA
TTGAGCGGCTTCAGAGCAAAGGAGCCAATCCCAAAGTGGCAGCGGCTCTGATGGCCTTGATGGATGACTCGCCTAATATC
GCCGGTCGGCCGCTATTCATCACATTACCGGTTGCCTCCCATGAGTTCGCCCAGTCACTGGGTCTGACACCGGAGACTCT
TTCGCGGTCGATCACACACCTCGTCGAAAAAAACATTCTCAAGCGTTTGCAGAGGAATCGATTTCAGATACTGGATTTAG
AAGCTCTTGAGAAAGTGGCAGAAAGTGCTCTGCGGTGA

Upstream 100 bases:

>100_bases
TTTCGCCACTATTTTCACCTGATGCAAAAGAGCTGTCAGCTCACCTGAAAGTGTAGATGTGTTTTACAAAAAAAACGGGA
CCCAACAAGTAGAGAATACA

Downstream 100 bases:

>100_bases
GGCAATCAGGCGCTTCGTTGCGCGCGGTCAATGGCATCCGATGTCTTCATCAATACTGATTGTCCCCACGATACGAGGGG
CTGAGAGAAGACGAGAGATC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MSLDNNKGKASHATRQILEKYLTLRSFRKGQLLWSEGDTDGLLVFLKSGRVKIYRLLPMGKAITLYIFGKGSVFGFMPFF
DDAPYPAYAQALDDCEADVISRSGLRQAVHQDPEVAIVLMKQLAQRLRDAFDTIERLQSKGANPKVAAALMALMDDSPNI
AGRPLFITLPVASHEFAQSLGLTPETLSRSITHLVEKNILKRLQRNRFQILDLEALEKVAESALR

Sequences:

>Translated_225_residues
MSLDNNKGKASHATRQILEKYLTLRSFRKGQLLWSEGDTDGLLVFLKSGRVKIYRLLPMGKAITLYIFGKGSVFGFMPFF
DDAPYPAYAQALDDCEADVISRSGLRQAVHQDPEVAIVLMKQLAQRLRDAFDTIERLQSKGANPKVAAALMALMDDSPNI
AGRPLFITLPVASHEFAQSLGLTPETLSRSITHLVEKNILKRLQRNRFQILDLEALEKVAESALR
>Mature_224_residues
SLDNNKGKASHATRQILEKYLTLRSFRKGQLLWSEGDTDGLLVFLKSGRVKIYRLLPMGKAITLYIFGKGSVFGFMPFFD
DAPYPAYAQALDDCEADVISRSGLRQAVHQDPEVAIVLMKQLAQRLRDAFDTIERLQSKGANPKVAAALMALMDDSPNIA
GRPLFITLPVASHEFAQSLGLTPETLSRSITHLVEKNILKRLQRNRFQILDLEALEKVAESALR

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25131; Mature: 24999

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLDNNKGKASHATRQILEKYLTLRSFRKGQLLWSEGDTDGLLVFLKSGRVKIYRLLPMG
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCEEEEEEECCC
KAITLYIFGKGSVFGFMPFFDDAPYPAYAQALDDCEADVISRSGLRQAVHQDPEVAIVLM
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KQLAQRLRDAFDTIERLQSKGANPKVAAALMALMDDSPNIAGRPLFITLPVASHEFAQSL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
GLTPETLSRSITHLVEKNILKRLQRNRFQILDLEALEKVAESALR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCC
>Mature Secondary Structure 
SLDNNKGKASHATRQILEKYLTLRSFRKGQLLWSEGDTDGLLVFLKSGRVKIYRLLPMG
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCEEEEEEECCC
KAITLYIFGKGSVFGFMPFFDDAPYPAYAQALDDCEADVISRSGLRQAVHQDPEVAIVLM
CEEEEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
KQLAQRLRDAFDTIERLQSKGANPKVAAALMALMDDSPNIAGRPLFITLPVASHEFAQSL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHH
GLTPETLSRSITHLVEKNILKRLQRNRFQILDLEALEKVAESALR
CCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]