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The map label for this gene is nth [H]

Identifier: 21674527

GI number: 21674527

Start: 1621681

End: 1622322

Strand: Direct

Name: nth [H]

Synonym: CT1710

Alternate gene names: 21674527

Gene position: 1621681-1622322 (Clockwise)

Preceding gene: 21674526

Following gene: 21674531

Centisome position: 75.25

GC content: 56.7

Gene sequence:

>642_bases
ATGACCACTTCCGTGAGCGAAAAGATCGCCTTTATCGAAAAGGCGCTGTCGGTGATCTGGCCGAACCCGAAAAGCGAGCT
GAACTTCGAAAGCCCTTTTCAGTTGCTGGTCGCCACCATCATGGCCGCGCAGGCCACGGACAAAAAGGTCAACGAACTCA
CCGCCGTACTCTTCAAAGCCGCGCCTGACGCCGCGAGCATGAGCCGGATGGATGTGGAGGATATCAGGACGATTATCCGC
CCGATCAACTACTACAACAACAAGGCGAAAAATATTCTCGCCATGAGCCGGCGCCTCGTCGATGAGTTCGGTGGCGAGGT
TCCTGCGTCACGCGAAGCACTGGAAAGCCTGCCGGGTGTGGGCAGGAAAACCGCCAACGTGGTGCTCGGCAACGCCTTCG
GCATCCCCGCCATGCCGGTGGACACTCACGTCCACCGCGTCTCGAACCGCATTGGCCTCTGCAAAACCTCGAAGCCTGAA
GAGACCGAAGAGGCACTGGTCAAAGTCATTCCGGAAGAAAAACTTATCGACTTCCACCACTACCTGCTGCTGCACGGCCG
CTACACCTGCAAAGCAAAAAAGCCCGAATGCGCGAACTGCGCGATTCGGGAAATCTGCGAATGGCCTGAAAAAACGTTGT
AA

Upstream 100 bases:

>100_bases
TGAAGGACGGATCAAAAAGTGCGGACGAGGCCTGGTCGACCACTCCGAACAGAAAATGCAGAAAACGGAAAACAACCGGT
AACCAGCTCTTTCAACAACG

Downstream 100 bases:

>100_bases
GAGAAGAGCATTCTTCTGAAAATCACTCTTCTCTTCATCCTTCAGGCTCACCTCTTCTTACGGCAGCTCTATCTTTTTAT
CCACCGCATACATCCACTCG

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MTTSVSEKIAFIEKALSVIWPNPKSELNFESPFQLLVATIMAAQATDKKVNELTAVLFKAAPDAASMSRMDVEDIRTIIR
PINYYNNKAKNILAMSRRLVDEFGGEVPASREALESLPGVGRKTANVVLGNAFGIPAMPVDTHVHRVSNRIGLCKTSKPE
ETEEALVKVIPEEKLIDFHHYLLLHGRYTCKAKKPECANCAIREICEWPEKTL

Sequences:

>Translated_213_residues
MTTSVSEKIAFIEKALSVIWPNPKSELNFESPFQLLVATIMAAQATDKKVNELTAVLFKAAPDAASMSRMDVEDIRTIIR
PINYYNNKAKNILAMSRRLVDEFGGEVPASREALESLPGVGRKTANVVLGNAFGIPAMPVDTHVHRVSNRIGLCKTSKPE
ETEEALVKVIPEEKLIDFHHYLLLHGRYTCKAKKPECANCAIREICEWPEKTL
>Mature_212_residues
TTSVSEKIAFIEKALSVIWPNPKSELNFESPFQLLVATIMAAQATDKKVNELTAVLFKAAPDAASMSRMDVEDIRTIIRP
INYYNNKAKNILAMSRRLVDEFGGEVPASREALESLPGVGRKTANVVLGNAFGIPAMPVDTHVHRVSNRIGLCKTSKPEE
TEEALVKVIPEEKLIDFHHYLLLHGRYTCKAKKPECANCAIREICEWPEKTL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA dam

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=31.638418079096, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787920, Length=191, Percent_Identity=52.3560209424084, Blast_Score=213, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17554540, Length=179, Percent_Identity=36.8715083798883, Blast_Score=122, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=26.2032085561497, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45550361, Length=177, Percent_Identity=32.7683615819209, Blast_Score=105, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23720; Mature: 23589

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSVSEKIAFIEKALSVIWPNPKSELNFESPFQLLVATIMAAQATDKKVNELTAVLFKA
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDAASMSRMDVEDIRTIIRPINYYNNKAKNILAMSRRLVDEFGGEVPASREALESLPGV
CCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
GRKTANVVLGNAFGIPAMPVDTHVHRVSNRIGLCKTSKPEETEEALVKVIPEEKLIDFHH
CCCHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
YLLLHGRYTCKAKKPECANCAIREICEWPEKTL
HHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TTSVSEKIAFIEKALSVIWPNPKSELNFESPFQLLVATIMAAQATDKKVNELTAVLFKA
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
APDAASMSRMDVEDIRTIIRPINYYNNKAKNILAMSRRLVDEFGGEVPASREALESLPGV
CCCHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
GRKTANVVLGNAFGIPAMPVDTHVHRVSNRIGLCKTSKPEETEEALVKVIPEEKLIDFHH
CCCHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHH
YLLLHGRYTCKAKKPECANCAIREICEWPEKTL
HHHHHCCEEECCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]