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The map label for this gene is purD

Identifier: 21674492

GI number: 21674492

Start: 1584696

End: 1585973

Strand: Reverse

Name: purD

Synonym: CT1674

Alternate gene names: 21674492

Gene position: 1585973-1584696 (Counterclockwise)

Preceding gene: 21674495

Following gene: 21674491

Centisome position: 73.6

GC content: 63.62

Gene sequence:

>1278_bases
ATGAAGGTTCTGATTATCGGAAGCGGCGCCAGGGAACATGCCATGGCCTGGGCCGTCGCGCGCAGCAGCAAGGTTTCGAC
CGTTTTCGTGGCCCCCGGTAACGGCGGCACGGCCACGATGGGCGGCAAGGTGCGTAACACGCCGGTTAAGGCGACCGACA
TCGACGCGCTGCTCGAACTGGTCGCGAAAGAGTCGATTGGTCTGACGGTGGTCGGCCCGGAGCAGCCGCTCGAAGCGGGC
ATCGTCAACCGGTTCCGCGAGGCGGGCTTCAAGGTGGTTGGCCCGACCGCCGAGGCAGCGCAACTGGAGACGAGCAAGGT
GTTCGCCAAGGAGTTTATGAAGCGCCACGGCATTCCGACCGCTGGGTACGAGGTGTTCCGCGATTATGCGTCCGCGAAGG
CGTTCCTCGAAACGTGCCCGACCTTTCCGCAAGTGATCAAGGCGAGCGGCCTCTGCGCGGGCAAGGGTGTCGTCGTTGCG
ATGAGCCGCGATGAGGCTCTTGAGGCGATCCACGAATTTTTCGAGTCGCGCATCTTCGGTGATGCTGCCGACGAGGTGGT
GATCGAGGCGTTCCTCTCTGGCCAGGAGGCGAGTGTGTTCGCCCTGACCGATGGTCAAAATTATCAGCTCTTCCTTTCTG
CGCAGGATCACAAGCGGATCGGCGAGGGCGATACCGGCAAGAACACTGGCGGCATGGGGGCCTACGCACCGGCTCCGCTG
GTGACGCCGGAGGTGATGCGGCGGGTCGAGGAGGAGGTTATCCGCCCGACGCTCGCCGGGATGAGGGCCGACGGATATGC
CTATACCGGTTTTCTCTACGTCGGCCTCATGATCGACAAGGGCGTGCCATCGGTGGTCGAGTACAACGCGCGGCTTGGCG
ATCCGGAGACGCAGGTGGTGCTGCCGATGCTGAAGAGCGACCTCTTCGACGCTCTGCTCGCGAGCGTCGAGGGCGGTCTC
GAAGTCGTGCCGTTTGAGATGCAGGAGGGCGCTGCTGCGACGGTGGTGATGGCCTCGGCGGGCTATCCCGACGCATACGA
GACCGGCAAGGTGATCACCATCGATCCGACAGTGAACGATATGGAGGGCGTGCTTGTTTTTCATGCCGGTACGCGCCGCG
ACGGCGATGCGCTGGTGACCTCCGGTGGCCGCGTGCTTTCAGTGACGGCCTGTGCCGGTTCGCTGAAAGAGGCGCTCGAT
CGGGTCTATCGCGCGGTGGATGCCATCGAATTCGAAGGCGCGTATTGCCGCCGCGACATTGGCGCGAAGGCGCTTTGA

Upstream 100 bases:

>100_bases
TATATACCTGCATTTGCCCGGCCCGCTCAGCCGGATGCAATTTAACAATCCACGCCGACAAACCACAGGGTGATTTGAGG
CCATCACTTTATACGCAAAC

Downstream 100 bases:

>100_bases
ATCATTCGGGACATTTTTGTAGTGGGCTGTACATGAAATTACCTCGTCGCAAGTTTGAGATCATCCAGGAGCATGCCGTC
AGGGATCTTCCCTACGAGTG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKVLIIGSGAREHAMAWAVARSSKVSTVFVAPGNGGTATMGGKVRNTPVKATDIDALLELVAKESIGLTVVGPEQPLEAG
IVNRFREAGFKVVGPTAEAAQLETSKVFAKEFMKRHGIPTAGYEVFRDYASAKAFLETCPTFPQVIKASGLCAGKGVVVA
MSRDEALEAIHEFFESRIFGDAADEVVIEAFLSGQEASVFALTDGQNYQLFLSAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIRPTLAGMRADGYAYTGFLYVGLMIDKGVPSVVEYNARLGDPETQVVLPMLKSDLFDALLASVEGGL
EVVPFEMQEGAAATVVMASAGYPDAYETGKVITIDPTVNDMEGVLVFHAGTRRDGDALVTSGGRVLSVTACAGSLKEALD
RVYRAVDAIEFEGAYCRRDIGAKAL

Sequences:

>Translated_425_residues
MKVLIIGSGAREHAMAWAVARSSKVSTVFVAPGNGGTATMGGKVRNTPVKATDIDALLELVAKESIGLTVVGPEQPLEAG
IVNRFREAGFKVVGPTAEAAQLETSKVFAKEFMKRHGIPTAGYEVFRDYASAKAFLETCPTFPQVIKASGLCAGKGVVVA
MSRDEALEAIHEFFESRIFGDAADEVVIEAFLSGQEASVFALTDGQNYQLFLSAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIRPTLAGMRADGYAYTGFLYVGLMIDKGVPSVVEYNARLGDPETQVVLPMLKSDLFDALLASVEGGL
EVVPFEMQEGAAATVVMASAGYPDAYETGKVITIDPTVNDMEGVLVFHAGTRRDGDALVTSGGRVLSVTACAGSLKEALD
RVYRAVDAIEFEGAYCRRDIGAKAL
>Mature_425_residues
MKVLIIGSGAREHAMAWAVARSSKVSTVFVAPGNGGTATMGGKVRNTPVKATDIDALLELVAKESIGLTVVGPEQPLEAG
IVNRFREAGFKVVGPTAEAAQLETSKVFAKEFMKRHGIPTAGYEVFRDYASAKAFLETCPTFPQVIKASGLCAGKGVVVA
MSRDEALEAIHEFFESRIFGDAADEVVIEAFLSGQEASVFALTDGQNYQLFLSAQDHKRIGEGDTGKNTGGMGAYAPAPL
VTPEVMRRVEEEVIRPTLAGMRADGYAYTGFLYVGLMIDKGVPSVVEYNARLGDPETQVVLPMLKSDLFDALLASVEGGL
EVVPFEMQEGAAATVVMASAGYPDAYETGKVITIDPTVNDMEGVLVFHAGTRRDGDALVTSGGRVLSVTACAGSLKEALD
RVYRAVDAIEFEGAYCRRDIGAKAL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=48.2352941176471, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=48.2352941176471, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=48.2352941176471, Blast_Score=391, Evalue=1e-109,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=48.2352941176471, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1790438, Length=427, Percent_Identity=51.288056206089, Blast_Score=410, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17567511, Length=425, Percent_Identity=39.0588235294118, Blast_Score=290, Evalue=1e-78,
Organism=Saccharomyces cerevisiae, GI6321203, Length=440, Percent_Identity=43.6363636363636, Blast_Score=352, Evalue=4e-98,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=43.6194895591647, Blast_Score=331, Evalue=6e-91,
Organism=Drosophila melanogaster, GI24582400, Length=431, Percent_Identity=43.6194895591647, Blast_Score=330, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_CHLTE (Q8KBV8)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662557.1
- ProteinModelPortal:   Q8KBV8
- SMR:   Q8KBV8
- GeneID:   1006158
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1674
- NMPDR:   fig|194439.1.peg.1651
- TIGR:   CT1674
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   CTEP194439:CT_1674-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 45065; Mature: 45065

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLIIGSGAREHAMAWAVARSSKVSTVFVAPGNGGTATMGGKVRNTPVKATDIDALLEL
CEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCHHHHHHH
VAKESIGLTVVGPEQPLEAGIVNRFREAGFKVVGPTAEAAQLETSKVFAKEFMKRHGIPT
HHHHCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCH
AGYEVFRDYASAKAFLETCPTFPQVIKASGLCAGKGVVVAMSRDEALEAIHEFFESRIFG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
DAADEVVIEAFLSGQEASVFALTDGQNYQLFLSAQDHKRIGEGDTGKNTGGMGAYAPAPL
CHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPEVMRRVEEEVIRPTLAGMRADGYAYTGFLYVGLMIDKGVPSVVEYNARLGDPETQVV
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEHCCCCCCHHHHHCCCCCCCCHHEE
LPMLKSDLFDALLASVEGGLEVVPFEMQEGAAATVVMASAGYPDAYETGKVITIDPTVND
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCHHHCCEEEEECCCCCC
MEGVLVFHAGTRRDGDALVTSGGRVLSVTACAGSLKEALDRVYRAVDAIEFEGAYCRRDI
CCCEEEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GAKAL
CCCCC
>Mature Secondary Structure
MKVLIIGSGAREHAMAWAVARSSKVSTVFVAPGNGGTATMGGKVRNTPVKATDIDALLEL
CEEEEEECCCHHHHHHHHHHCCCCCEEEEEECCCCCCEECCCEEECCCCCCCCHHHHHHH
VAKESIGLTVVGPEQPLEAGIVNRFREAGFKVVGPTAEAAQLETSKVFAKEFMKRHGIPT
HHHHCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCH
AGYEVFRDYASAKAFLETCPTFPQVIKASGLCAGKGVVVAMSRDEALEAIHEFFESRIFG
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHCC
DAADEVVIEAFLSGQEASVFALTDGQNYQLFLSAQDHKRIGEGDTGKNTGGMGAYAPAPL
CHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
VTPEVMRRVEEEVIRPTLAGMRADGYAYTGFLYVGLMIDKGVPSVVEYNARLGDPETQVV
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEEEEEHCCCCCCHHHHHCCCCCCCCHHEE
LPMLKSDLFDALLASVEGGLEVVPFEMQEGAAATVVMASAGYPDAYETGKVITIDPTVND
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCHHHCCEEEEECCCCCC
MEGVLVFHAGTRRDGDALVTSGGRVLSVTACAGSLKEALDRVYRAVDAIEFEGAYCRRDI
CCCEEEEECCCCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCC
GAKAL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901