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The map label for this gene is carB1 [H]

Identifier: 21674490

GI number: 21674490

Start: 1582612

End: 1584096

Strand: Reverse

Name: carB1 [H]

Synonym: CT1672

Alternate gene names: 21674490

Gene position: 1584096-1582612 (Counterclockwise)

Preceding gene: 21674491

Following gene: 21674489

Centisome position: 73.51

GC content: 58.92

Gene sequence:

>1485_bases
GTGCCAAAACGCGAGGACATCAAGTCTATTCTGGTGATTGGAGCTGGCCCGATTGTTATCGGGCAGGCGTGTGAGTTTGA
TTATTCAGGCACCCAGGCGTGCCGTGCGCTCAAGGAGGATGGTTACCGGGTCATTCTGGTCAACAGCAATCCCGCGACGA
TCATGACCGATATCGAGCTGGCCGATGCCACCTACATCGAGCCGATCACGCCCTACTATGTCACCAAGATCATCGAAAAG
GAGAAGCCGGACGCGCTGCTACCGACCATGGGCGGTCAGACCGCGCTGAACACGGCGGTGAAGTTGGCCGAGTCGGGCGT
GCTCGAACGCCACGGTGTCGAGCTGATCGGTGCGAAGTTCCGCGCCATCCGCAAGGCTGAGAATCGCGAGTTCTTCGGCG
ATGCGATGCGAAAGCTCGGCCTTGAAATGGCCAAGGGTTTCTTCGTTCGCAACGAGAAAGAGGCCAAGGAGGCGCTTGAA
GAGATCGGTCTGCCGATTGTCATTCGCCCCTCTTTTACGCTCGGCGGCACGGGTGGCGGTTTCGCCGAGACCAAGGCCGA
CTACTACGACATGGTGCGGCGCGGTCTGGCCGAGAGCCCAATTGGCGAGGTGCTGGTCGAGGAGAGTCTGATCGGCTGGA
AGGAGTACGAGCTTGAGGTGATCCGCGACCTGGCCGACAACGTCATCATCGTCTGCTCCATCGAGAACGTCGATCCGATG
GGCGTGCATACCGGCGACAGCATCACGGTCGCTCCGGCGCAGACGCTCACCGACCGCCAGTATCAGGAGCTGCGTGACGC
CTCGATCAAAATCATCCGCGAAATCGGCGTCGAGACCGGCGGCAGTAACATCCAGTTCGCGATCAACCCGAAGAGTGGCC
GCATCGTGGTCATCGAGATGAATCCTCGCGTGTCGAGAAGCTCCGCGCTTGCCTCGAAGGCGACTGGCTTCCCGATTGCC
AAGGTAGCTGCCAAGCTCGCGGTAGGCTATACGCTCGACGAGATTACCAACGACATCACCAAGACGACGCCCGCAAGCTT
CGAGCCTTCGATTGACTACACCGTGGTGAAGGTGCCTCGCTGGGATTTCGAGAAGTTCAAGAATGTCGATTCGCGCCTCG
GCGTGCAGATGAAGTCGGTCGGCGAGGTGATGGCTTTCGGACGCAATTTTCGCGAGGCGCTCCAGAAGTCGCTTCGCGGT
CTCGAAATCGGTCGGGCCGGTCTGGGCTGCGATGGGAAGGATATCATGAACGTCATCGACATGACCCAGCAGCAGCGCAA
ATTCGCCAAAGAGGATCTGCTCGAAAAGATCAAGATTCCCAAGGCTGACCGGATGTTCTATCTCCGCTATGCATTCCAGG
CTGGCGCAACCGTCGAGGAGATTCATCAGGCGACCGGCATCGACCCGTGGTTCCTTGACAACATCCGACAGATCGTCGAA
TTCGAGGACGAACTTCGACAGATGGCAGCTGGAAGCGCGGCATGA

Upstream 100 bases:

>100_bases
TTCGTCCATCAAACCCACCGTGTTCCCTCAAAGAAGAGATGAAAATTTTTGTACATTACGCAACTGACAGTTTACCTCAA
GATTTAAAAGCGGAAGACTA

Downstream 100 bases:

>100_bases
CCTATCTTACCCGGATTCTCGAGACCAAGGCAAGAGAGGTTGCCGAACTGAAAAAGCTGAAACCGGAGCGCCGCTATCGT
GAGGCGTGCGGCGATCTTCC

Product: carbamoyl-phosphate synthase, medium subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase ammonia chain [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPYYVTKIIEK
EKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKFRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALE
EIGLPIVIRPSFTLGGTGGGFAETKADYYDMVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFAINPKSGRIVVIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRG
LEIGRAGLGCDGKDIMNVIDMTQQQRKFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATGIDPWFLDNIRQIVE
FEDELRQMAAGSAA

Sequences:

>Translated_494_residues
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPYYVTKIIEK
EKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKFRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALE
EIGLPIVIRPSFTLGGTGGGFAETKADYYDMVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFAINPKSGRIVVIEMNPRVSRSSALASKATGFPIA
KVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRG
LEIGRAGLGCDGKDIMNVIDMTQQQRKFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATGIDPWFLDNIRQIVE
FEDELRQMAAGSAA
>Mature_493_residues
PKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIELADATYIEPITPYYVTKIIEKE
KPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKFRAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALEE
IGLPIVIRPSFTLGGTGGGFAETKADYYDMVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPMG
VHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFAINPKSGRIVVIEMNPRVSRSSALASKATGFPIAK
VAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPRWDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGL
EIGRAGLGCDGKDIMNVIDMTQQQRKFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATGIDPWFLDNIRQIVEF
EDELRQMAAGSAA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0458

COG function: function code EF; Carbamoylphosphate synthase large subunit (split gene in MJ)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ATP-grasp domains [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=482, Percent_Identity=45.0207468879668, Blast_Score=424, Evalue=1e-118,
Organism=Homo sapiens, GI21361331, Length=492, Percent_Identity=42.2764227642276, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI169790915, Length=492, Percent_Identity=42.2764227642276, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI170295797, Length=459, Percent_Identity=41.6122004357298, Blast_Score=380, Evalue=1e-105,
Organism=Escherichia coli, GI1786216, Length=489, Percent_Identity=62.3721881390593, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI193204318, Length=481, Percent_Identity=45.7380457380457, Blast_Score=426, Evalue=1e-119,
Organism=Saccharomyces cerevisiae, GI6322569, Length=489, Percent_Identity=46.2167689161554, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322331, Length=483, Percent_Identity=44.5134575569358, Blast_Score=416, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24642586, Length=485, Percent_Identity=44.3298969072165, Blast_Score=431, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45555749, Length=485, Percent_Identity=44.3298969072165, Blast_Score=431, Evalue=1e-121,

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006275
- InterPro:   IPR005479
- InterPro:   IPR005483
- InterPro:   IPR005481
- InterPro:   IPR005480
- InterPro:   IPR011607
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF00289 CPSase_L_chain; PF02786 CPSase_L_D2; PF02787 CPSase_L_D3; PF02142 MGS [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 54545; Mature: 54414

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50975 ATP_GRASP ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIEL
CCCHHHHCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
ADATYIEPITPYYVTKIIEKEKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKF
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHH
RAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALEEIGLPIVIRPSFTLGGTGGG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEECCCCCC
FAETKADYYDMVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFAINPKSGRIVVIEM
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCC
WDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGLEIGRAGLGCDGKDIMNVID
CCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
MTQQQRKFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATGIDPWFLDNIRQIVE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
FEDELRQMAAGSAA
HHHHHHHHHCCCCC
>Mature Secondary Structure 
PKREDIKSILVIGAGPIVIGQACEFDYSGTQACRALKEDGYRVILVNSNPATIMTDIEL
CCHHHHCEEEEEECCCEEECCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEEE
ADATYIEPITPYYVTKIIEKEKPDALLPTMGGQTALNTAVKLAESGVLERHGVELIGAKF
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHH
RAIRKAENREFFGDAMRKLGLEMAKGFFVRNEKEAKEALEEIGLPIVIRPSFTLGGTGGG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCEEECCCCCC
FAETKADYYDMVRRGLAESPIGEVLVEESLIGWKEYELEVIRDLADNVIIVCSIENVDPM
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEEEECCCCCC
GVHTGDSITVAPAQTLTDRQYQELRDASIKIIREIGVETGGSNIQFAINPKSGRIVVIEM
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEC
NPRVSRSSALASKATGFPIAKVAAKLAVGYTLDEITNDITKTTPASFEPSIDYTVVKVPR
CCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCC
WDFEKFKNVDSRLGVQMKSVGEVMAFGRNFREALQKSLRGLEIGRAGLGCDGKDIMNVID
CCHHHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHH
MTQQQRKFAKEDLLEKIKIPKADRMFYLRYAFQAGATVEEIHQATGIDPWFLDNIRQIVE
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHHHHH
FEDELRQMAAGSAA
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]