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The map label for this gene is sppA [H]

Identifier: 21674462

GI number: 21674462

Start: 1554757

End: 1556550

Strand: Reverse

Name: sppA [H]

Synonym: CT1644

Alternate gene names: 21674462

Gene position: 1556550-1554757 (Counterclockwise)

Preceding gene: 21674463

Following gene: 21674460

Centisome position: 72.23

GC content: 58.36

Gene sequence:

>1794_bases
ATGGCAGACAAGAACCGAAAAAAAGGCGGATGTTTCCGCGCCGGGTGCCTGACGGCAGTCGTTGCCGTGCTTCTGCTCGT
TGGACTGGGAGGGTGGTTTGTGCATCAGAGGAGCAATCGTCTTCCCGCACGCTTCGTGCTCTCGGTGCCGTTGACCGGAG
AGCTCGATGAGCGCCCTCCGGATGCGGGTCCATTCCCCTTTGGCAAAGGACGCCATCTGCTCTCGTTTGAGGAATTGCTG
ACCATTCTTGACCGTGCGAAAACTGACCGCCGTGTCGATTCGGTGCTGCTCAGGATCGATGGTCTGGGTGCCTCGCCTGC
CAAGATACAGGAGCTGAGAAGTTCAATTGCCGCCTTGCGGAAATCGGGCAAAAAGGTCACAGCGTTTCTTGTCACGCCTG
AAGACAAGGATTATCAGCTCGCCGTCGCCTGCGATTCGATCATTGTACAGAAAGGTTCCTGGATGACGCTCGACGGGCTT
AAGGCCGAGCTGTTCTTTGTCGCCGATCCGCTCAAAAAGCTCGGTGTCAGCTTTCAGGCAGCGCAGTGGAAAAAGTATAA
AAGTGCCGTCGAAACCTTCACCAGAAATTCGGCCAGCCCGGAGAATCTCGAAGAGACCAACGCCTTGCTCGATGACGCCT
GGAGCGATTATCTCGACTCCGTATCGCGGCAGCGTCGCATCGGCAAGGATGCCTTCCGGAAGGTGGTTGACTCGCTGGCC
GTGTTGACGCCTGAAAAAGCGCTTGGGCTTCATTTGATCGATCGTGTGGCCACTGAACGCGAACTTGAACAAGAGTATGC
AAGGCGTTTGAACAAGCCAGCAGCGGAGCTGTTGGTGGGAGGCCGGGAGTATCTCGGCGCTACTGGCGGTATGCGTCCGC
AGGGAGGCGGCGACCGGATTGCTGTGATCAACATTACCGGCATGATCGTGAGCGACGGTGCGGGCGGTATGAGCGAGGGC
GATGGAACCGATGTCGCTACTGTCAAGGAGGCGCTTCAGACTGCGATCGACGACTTGAAGGTCAAGGCGATCGTGCTGCG
GATCGATAGCCCTGGCGGCGATGCCCTTGCCGCCTCCACGATGCTCGAACTGCTCAACGAGGCGAAAGCCAAAAAGCCGA
TTGTGGCCTCGATGTCCGGCTTGGCGGCTTCGGGTGGCTACATGGTGGCGCTCGCTGGCGACAAAATCTTTGCCGAACCG
CTCACCATAACCGGTTCGATTGGCGTTTTCTCGCTCAAGCCCGATTTGAGCAGCCTGCTCGAAAAGACCGGCATCCGGCG
CGAGGTGCTCATCCGGGGCCGCTTCGCTGACGCCGAAACGCCATTCAGAGCGTTTGACGACGCATCGTTCCGCAAGTTTG
TTGAGCTCACCGGTACGGTTTATGAGGATTTCATCGCCAAAGTGGCGAAAGGACGCCATATGACACCAGCGCAGGTTGAT
GCCGTGGCTGGAGGGCGGGTGTGGAGCGGCAAGCGCGCGCTGGAAGTTGGTCTCATCGACCAGATTGGTGGTCTTGGTGA
CGCCGTGCAGGAGGCCAAAAAGCTGGCCGGCGTGAAGAAGGAGGTCAAGCCGGCGCTGCTTTATCTGCCTGCCCAAAAAA
CATGGCTTGAATACCTGCTTGGCAGTGATACGTCGGATATGGTTTCGGCGTTGACTGCCGCAATGGTCAGGGAGTCGCTT
GGTCAATTGCAACCGCTCTCTCGCTTGCCCGGCGCCGGCATCGCCCGCTTTCTTTTGCGTACCGATGCCCCCCAGGTGCT
GGCGCTCGATCCGGTCGAAGTGGTGATAAAGTAG

Upstream 100 bases:

>100_bases
TGGAGGAGTGCCGGAAACGTGAGCCGGAACTGCTGTCGTTGCAAGATGAGCCATCACACAAAGTAAGCTGTCTTCTTTAC
CAGTAATTTATTGCGTCGAT

Downstream 100 bases:

>100_bases
GGGCGATCGGCTGATCGCCCCGGAAAAGGTCAGAACTCAGAACGAGTAGGTGACTGAAGTCTGGCGCGGACGGCCTCACC
CTCTTTGGCGTCTTTGTAGT

Product: protease IV

Products: NA

Alternate protein names: Endopeptidase IV; Protease IV homolog; Signal peptide peptidase [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MADKNRKKGGCFRAGCLTAVVAVLLLVGLGGWFVHQRSNRLPARFVLSVPLTGELDERPPDAGPFPFGKGRHLLSFEELL
TILDRAKTDRRVDSVLLRIDGLGASPAKIQELRSSIAALRKSGKKVTAFLVTPEDKDYQLAVACDSIIVQKGSWMTLDGL
KAELFFVADPLKKLGVSFQAAQWKKYKSAVETFTRNSASPENLEETNALLDDAWSDYLDSVSRQRRIGKDAFRKVVDSLA
VLTPEKALGLHLIDRVATERELEQEYARRLNKPAAELLVGGREYLGATGGMRPQGGGDRIAVINITGMIVSDGAGGMSEG
DGTDVATVKEALQTAIDDLKVKAIVLRIDSPGGDALAASTMLELLNEAKAKKPIVASMSGLAASGGYMVALAGDKIFAEP
LTITGSIGVFSLKPDLSSLLEKTGIRREVLIRGRFADAETPFRAFDDASFRKFVELTGTVYEDFIAKVAKGRHMTPAQVD
AVAGGRVWSGKRALEVGLIDQIGGLGDAVQEAKKLAGVKKEVKPALLYLPAQKTWLEYLLGSDTSDMVSALTAAMVRESL
GQLQPLSRLPGAGIARFLLRTDAPQVLALDPVEVVIK

Sequences:

>Translated_597_residues
MADKNRKKGGCFRAGCLTAVVAVLLLVGLGGWFVHQRSNRLPARFVLSVPLTGELDERPPDAGPFPFGKGRHLLSFEELL
TILDRAKTDRRVDSVLLRIDGLGASPAKIQELRSSIAALRKSGKKVTAFLVTPEDKDYQLAVACDSIIVQKGSWMTLDGL
KAELFFVADPLKKLGVSFQAAQWKKYKSAVETFTRNSASPENLEETNALLDDAWSDYLDSVSRQRRIGKDAFRKVVDSLA
VLTPEKALGLHLIDRVATERELEQEYARRLNKPAAELLVGGREYLGATGGMRPQGGGDRIAVINITGMIVSDGAGGMSEG
DGTDVATVKEALQTAIDDLKVKAIVLRIDSPGGDALAASTMLELLNEAKAKKPIVASMSGLAASGGYMVALAGDKIFAEP
LTITGSIGVFSLKPDLSSLLEKTGIRREVLIRGRFADAETPFRAFDDASFRKFVELTGTVYEDFIAKVAKGRHMTPAQVD
AVAGGRVWSGKRALEVGLIDQIGGLGDAVQEAKKLAGVKKEVKPALLYLPAQKTWLEYLLGSDTSDMVSALTAAMVRESL
GQLQPLSRLPGAGIARFLLRTDAPQVLALDPVEVVIK
>Mature_596_residues
ADKNRKKGGCFRAGCLTAVVAVLLLVGLGGWFVHQRSNRLPARFVLSVPLTGELDERPPDAGPFPFGKGRHLLSFEELLT
ILDRAKTDRRVDSVLLRIDGLGASPAKIQELRSSIAALRKSGKKVTAFLVTPEDKDYQLAVACDSIIVQKGSWMTLDGLK
AELFFVADPLKKLGVSFQAAQWKKYKSAVETFTRNSASPENLEETNALLDDAWSDYLDSVSRQRRIGKDAFRKVVDSLAV
LTPEKALGLHLIDRVATERELEQEYARRLNKPAAELLVGGREYLGATGGMRPQGGGDRIAVINITGMIVSDGAGGMSEGD
GTDVATVKEALQTAIDDLKVKAIVLRIDSPGGDALAASTMLELLNEAKAKKPIVASMSGLAASGGYMVALAGDKIFAEPL
TITGSIGVFSLKPDLSSLLEKTGIRREVLIRGRFADAETPFRAFDDASFRKFVELTGTVYEDFIAKVAKGRHMTPAQVDA
VAGGRVWSGKRALEVGLIDQIGGLGDAVQEAKKLAGVKKEVKPALLYLPAQKTWLEYLLGSDTSDMVSALTAAMVRESLG
QLQPLSRLPGAGIARFLLRTDAPQVLALDPVEVVIK

Specific function: Digestion of the cleaved signal peptides. This activity is necessary to maintain proper secretion of mature proteins across the membrane [H]

COG id: COG0616

COG function: function code OU; Periplasmic serine proteases (ClpP class)

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S49 family [H]

Homologues:

Organism=Escherichia coli, GI1788064, Length=574, Percent_Identity=27.8745644599303, Blast_Score=179, Evalue=6e-46,
Organism=Escherichia coli, GI1787527, Length=203, Percent_Identity=31.0344827586207, Blast_Score=89, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004634
- InterPro:   IPR004635
- InterPro:   IPR002142 [H]

Pfam domain/function: PF01343 Peptidase_S49 [H]

EC number: 3.4.21.-

Molecular weight: Translated: 64209; Mature: 64078

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKNRKKGGCFRAGCLTAVVAVLLLVGLGGWFVHQRSNRLPARFVLSVPLTGELDERPP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCC
DAGPFPFGKGRHLLSFEELLTILDRAKTDRRVDSVLLRIDGLGASPAKIQELRSSIAALR
CCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHH
KSGKKVTAFLVTPEDKDYQLAVACDSIIVQKGSWMTLDGLKAELFFVADPLKKLGVSFQA
HCCCEEEEEEECCCCCCCEEEEEECHHHEECCCEEEECCCCEEEEEEECHHHHHCCCCHH
AQWKKYKSAVETFTRNSASPENLEETNALLDDAWSDYLDSVSRQRRIGKDAFRKVVDSLA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VLTPEKALGLHLIDRVATERELEQEYARRLNKPAAELLVGGREYLGATGGMRPQGGGDRI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCCCCCCCCCEE
AVINITGMIVSDGAGGMSEGDGTDVATVKEALQTAIDDLKVKAIVLRIDSPGGDALAAST
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHH
MLELLNEAKAKKPIVASMSGLAASGGYMVALAGDKIFAEPLTITGSIGVFSLKPDLSSLL
HHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCEEEECCEEEEECCEEEEECHHHHHHH
EKTGIRREVLIRGRFADAETPFRAFDDASFRKFVELTGTVYEDFIAKVAKGRHMTPAQVD
HHHCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AVAGGRVWSGKRALEVGLIDQIGGLGDAVQEAKKLAGVKKEVKPALLYLPAQKTWLEYLL
HCCCCCEECCCCCEEECCHHHHCCCHHHHHHHHHHHCHHHHCCCEEEEECCHHHHHHHHH
GSDTSDMVSALTAAMVRESLGQLQPLSRLPGAGIARFLLRTDAPQVLALDPVEVVIK
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCEEEECHHHHHCC
>Mature Secondary Structure 
ADKNRKKGGCFRAGCLTAVVAVLLLVGLGGWFVHQRSNRLPARFVLSVPLTGELDERPP
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCC
DAGPFPFGKGRHLLSFEELLTILDRAKTDRRVDSVLLRIDGLGASPAKIQELRSSIAALR
CCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHH
KSGKKVTAFLVTPEDKDYQLAVACDSIIVQKGSWMTLDGLKAELFFVADPLKKLGVSFQA
HCCCEEEEEEECCCCCCCEEEEEECHHHEECCCEEEECCCCEEEEEEECHHHHHCCCCHH
AQWKKYKSAVETFTRNSASPENLEETNALLDDAWSDYLDSVSRQRRIGKDAFRKVVDSLA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VLTPEKALGLHLIDRVATERELEQEYARRLNKPAAELLVGGREYLGATGGMRPQGGGDRI
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHCCCCCCCCCCCCCEE
AVINITGMIVSDGAGGMSEGDGTDVATVKEALQTAIDDLKVKAIVLRIDSPGGDALAAST
EEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHH
MLELLNEAKAKKPIVASMSGLAASGGYMVALAGDKIFAEPLTITGSIGVFSLKPDLSSLL
HHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEECCEEEECCEEEEECCEEEEECHHHHHHH
EKTGIRREVLIRGRFADAETPFRAFDDASFRKFVELTGTVYEDFIAKVAKGRHMTPAQVD
HHHCCCEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
AVAGGRVWSGKRALEVGLIDQIGGLGDAVQEAKKLAGVKKEVKPALLYLPAQKTWLEYLL
HCCCCCEECCCCCEEECCHHHHCCCHHHHHHHHHHHCHHHHCCCEEEEECCHHHHHHHHH
GSDTSDMVSALTAAMVRESLGQLQPLSRLPGAGIARFLLRTDAPQVLALDPVEVVIK
CCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHCCCCCEEEECHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]