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The map label for this gene is dnaG [H]

Identifier: 21674406

GI number: 21674406

Start: 1494185

End: 1496062

Strand: Direct

Name: dnaG [H]

Synonym: CT1588

Alternate gene names: 21674406

Gene position: 1494185-1496062 (Clockwise)

Preceding gene: 21674405

Following gene: 21674410

Centisome position: 69.34

GC content: 60.6

Gene sequence:

>1878_bases
ATGATTGATGAGGTCCGCCAGAGCACGGACATTGTTGACGTGGTGTCGGATTATGTCAGGCTGCACCCCTCGGGCCGCCA
TTTCAAGGCGCTCTCGCCCTTTACGCAGGAGAAGACCCCGTCGTTCATCGTCTCGCCCGACAAGCAGATTTACAAATGCT
TTTCGAGCGGCAAGGGCGGCAACGTCTTCACCTTCGTCATGGAGATGGAGAAGGTGAGCTTTCCTGAAGCGGTCGAGATG
CTGGCCAAACGCGCCGGAATCGACATCGGCAAATACCAGCAACAGAAGGCCAAAGAAAAGGATAAACGGGAGGCCAGCCA
GTTCGACACACTGCGCTGGGCGGCGAAGCTGTTCCACGGCACGCTGCAAAGCGAGGCCGGAAGCGCGGCGCTCGCCTACC
TCACCGGCAAGCGGGGACTCGAACCCGGCACGATCCGGCGCTTCGGCCTCGGCTTCGCGCCCGAGTCGTGGGATCACCTG
CTCCATGCGGCGGAGCGTGACGGAGCACCGGTCGAGCATCTCGTCAGCCTCGGCCTCCTGACGCGCCACCCGAAGCGCAA
CACTCTCTACGACACATTCCGCAACCGGATCATCTTTCCGATTCTGACCGTCGGCGGCCAGGTGGCGGGCTTCGGCGGGC
GTACGCTGTCAAACGACGCCGAAACACCCAAGTACATCAACTCGCCGGAGAGCGCCTTCTTCGAGAAGTCGAAGCTGCTC
TACGGGATGCACGCCGCCAAGAACGAAATCCGGCGGCAGGAAACGGCCATTCTCGTCGAAGGGTACATGGACGTGATCGC
CATGCACCAGGCGGGCTTCACCAACACGGTCGCCTCGTGCGGCACGGCGCTCACCCGCTACCAGGCCAAAATCCTGAAGC
GCTACACCTCGCGCGTGCTCTTCCTCTACGACGGCGACAACGCAGGCAAGAAATCGATGCTGGCGGGCATCGACATTCTG
CTCTCGGAGGGGCTGACGCCGTGGGTGGTGATGCTGCCCGGCAATGAAGACCCCGACAGTTTCATTCGCAACTACGGCAA
GGAGGCGTTTCTCGGCGAGCTGGAGAGTGAAAAAAGCTCGTTTCAGGATTTCCAGCTCCGCTGCTATCAGGATGCCGGAT
GGATGGAGAGCCCCGATACCGCCTCAAAAGCGATTTCTGCCATGACGCGGAGCATCGCCCTCATCGCCGATCCGGTGCAG
AAGGAACTGTACATCGACGAGCTGTCGAAAAAGCTCGATCTCAGCCGCCAGACGCTCCGCGAGGTGATGGCCGCGAGCGA
CACCGGGGGCAGCACGCGAGAGTCACGCCAGCGCCGCAGCGAACCGGCCCCGCAGCCCCTCGCGCGGCAAGCGCCGCTCT
CCGTGACGGAACGCACCTTCCTCGAAGCGCTGATCGAAAGCACCTTCTACGGCAACGAAGTGCTCGACTTCGCCGCCTCG
CATGAGTCGATGTTCCACCTCGAACATCCTGCCGCGCAAACAATTTTCAGCCATCTGGTCAGGCGATTCCGCGAAATGAA
TGACCGCGACGGTCACCTCGACATCAACTCTGAGATCAGCTCCATCGGCATGGAGGACGCAAGCAACCTCGCCTTCGACA
TCCTCTTCCGCCTGCCGGTCAACGAAACCACGCGCCTGACCCCTGCCGAACTCGAACAGCACGCCCGCCGTTGCCTCTCT
CACTTCCTCGTTGCGGTCAAAGCGCTGGTGCTCGAACCGTTGCAGAAAGAGAAACAGAAAATTATCGCACAACTCCAGTC
AGCCACCAGCACGCACGAACAGGAACGCTTGTCAAGAGAGCTTCTCGAACACAACAAACAATTTCGCGCGATGGAGCAGG
AGGTTGACGAGTCGATCAGGGGAATATTGGGGGAATAA

Upstream 100 bases:

>100_bases
ACGGCTCGCAAGGCATTGCGATTTCTTTATTTTCAATTTATCATAGCATCGAACAAAACGGGAAAAGAATGAGAACATTA
CAGGCATGATACCGGAACGC

Downstream 100 bases:

>100_bases
AATTTCGCCTGTTCAACAGCTTATAACAATTGCCTTTTATCCAGCAATAAAAGGCGATCTATGAACAGGAGAAATATGGC
TATACAAAAGTCCGTCCGTA

Product: DNA primase, putative

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MIDEVRQSTDIVDVVSDYVRLHPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFVMEMEKVSFPEAVEM
LAKRAGIDIGKYQQQKAKEKDKREASQFDTLRWAAKLFHGTLQSEAGSAALAYLTGKRGLEPGTIRRFGLGFAPESWDHL
LHAAERDGAPVEHLVSLGLLTRHPKRNTLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDAETPKYINSPESAFFEKSKLL
YGMHAAKNEIRRQETAILVEGYMDVIAMHQAGFTNTVASCGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLAGIDIL
LSEGLTPWVVMLPGNEDPDSFIRNYGKEAFLGELESEKSSFQDFQLRCYQDAGWMESPDTASKAISAMTRSIALIADPVQ
KELYIDELSKKLDLSRQTLREVMAASDTGGSTRESRQRRSEPAPQPLARQAPLSVTERTFLEALIESTFYGNEVLDFAAS
HESMFHLEHPAAQTIFSHLVRRFREMNDRDGHLDINSEISSIGMEDASNLAFDILFRLPVNETTRLTPAELEQHARRCLS
HFLVAVKALVLEPLQKEKQKIIAQLQSATSTHEQERLSRELLEHNKQFRAMEQEVDESIRGILGE

Sequences:

>Translated_625_residues
MIDEVRQSTDIVDVVSDYVRLHPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFVMEMEKVSFPEAVEM
LAKRAGIDIGKYQQQKAKEKDKREASQFDTLRWAAKLFHGTLQSEAGSAALAYLTGKRGLEPGTIRRFGLGFAPESWDHL
LHAAERDGAPVEHLVSLGLLTRHPKRNTLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDAETPKYINSPESAFFEKSKLL
YGMHAAKNEIRRQETAILVEGYMDVIAMHQAGFTNTVASCGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLAGIDIL
LSEGLTPWVVMLPGNEDPDSFIRNYGKEAFLGELESEKSSFQDFQLRCYQDAGWMESPDTASKAISAMTRSIALIADPVQ
KELYIDELSKKLDLSRQTLREVMAASDTGGSTRESRQRRSEPAPQPLARQAPLSVTERTFLEALIESTFYGNEVLDFAAS
HESMFHLEHPAAQTIFSHLVRRFREMNDRDGHLDINSEISSIGMEDASNLAFDILFRLPVNETTRLTPAELEQHARRCLS
HFLVAVKALVLEPLQKEKQKIIAQLQSATSTHEQERLSRELLEHNKQFRAMEQEVDESIRGILGE
>Mature_625_residues
MIDEVRQSTDIVDVVSDYVRLHPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGGNVFTFVMEMEKVSFPEAVEM
LAKRAGIDIGKYQQQKAKEKDKREASQFDTLRWAAKLFHGTLQSEAGSAALAYLTGKRGLEPGTIRRFGLGFAPESWDHL
LHAAERDGAPVEHLVSLGLLTRHPKRNTLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDAETPKYINSPESAFFEKSKLL
YGMHAAKNEIRRQETAILVEGYMDVIAMHQAGFTNTVASCGTALTRYQAKILKRYTSRVLFLYDGDNAGKKSMLAGIDIL
LSEGLTPWVVMLPGNEDPDSFIRNYGKEAFLGELESEKSSFQDFQLRCYQDAGWMESPDTASKAISAMTRSIALIADPVQ
KELYIDELSKKLDLSRQTLREVMAASDTGGSTRESRQRRSEPAPQPLARQAPLSVTERTFLEALIESTFYGNEVLDFAAS
HESMFHLEHPAAQTIFSHLVRRFREMNDRDGHLDINSEISSIGMEDASNLAFDILFRLPVNETTRLTPAELEQHARRCLS
HFLVAVKALVLEPLQKEKQKIIAQLQSATSTHEQERLSRELLEHNKQFRAMEQEVDESIRGILGE

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=359, Percent_Identity=37.3259052924791, Blast_Score=241, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70222; Mature: 70222

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDEVRQSTDIVDVVSDYVRLHPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCC
NVFTFVMEMEKVSFPEAVEMLAKRAGIDIGKYQQQKAKEKDKREASQFDTLRWAAKLFHG
CEEEEEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQSEAGSAALAYLTGKRGLEPGTIRRFGLGFAPESWDHLLHAAERDGAPVEHLVSLGLL
HHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
TRHPKRNTLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDAETPKYINSPESAFFEKSKLL
HCCCCCCHHHHHHHCCEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YGMHAAKNEIRRQETAILVEGYMDVIAMHQAGFTNTVASCGTALTRYQAKILKRYTSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
FLYDGDNAGKKSMLAGIDILLSEGLTPWVVMLPGNEDPDSFIRNYGKEAFLGELESEKSS
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
FQDFQLRCYQDAGWMESPDTASKAISAMTRSIALIADPVQKELYIDELSKKLDLSRQTLR
HHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVMAASDTGGSTRESRQRRSEPAPQPLARQAPLSVTERTFLEALIESTFYGNEVLDFAAS
HHHHHCCCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHC
HESMFHLEHPAAQTIFSHLVRRFREMNDRDGHLDINSEISSIGMEDASNLAFDILFRLPV
CCHHEEECCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCCCCHHHHHHHHHHEECCC
NETTRLTPAELEQHARRCLSHFLVAVKALVLEPLQKEKQKIIAQLQSATSTHEQERLSRE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEHNKQFRAMEQEVDESIRGILGE
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIDEVRQSTDIVDVVSDYVRLHPSGRHFKALSPFTQEKTPSFIVSPDKQIYKCFSSGKGG
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHCCCCC
NVFTFVMEMEKVSFPEAVEMLAKRAGIDIGKYQQQKAKEKDKREASQFDTLRWAAKLFHG
CEEEEEEEHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLQSEAGSAALAYLTGKRGLEPGTIRRFGLGFAPESWDHLLHAAERDGAPVEHLVSLGLL
HHHCCCCCEEEEEEECCCCCCCCCHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
TRHPKRNTLYDTFRNRIIFPILTVGGQVAGFGGRTLSNDAETPKYINSPESAFFEKSKLL
HCCCCCCHHHHHHHCCEEHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
YGMHAAKNEIRRQETAILVEGYMDVIAMHQAGFTNTVASCGTALTRYQAKILKRYTSRVL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
FLYDGDNAGKKSMLAGIDILLSEGLTPWVVMLPGNEDPDSFIRNYGKEAFLGELESEKSS
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHH
FQDFQLRCYQDAGWMESPDTASKAISAMTRSIALIADPVQKELYIDELSKKLDLSRQTLR
HHHHHHHEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVMAASDTGGSTRESRQRRSEPAPQPLARQAPLSVTERTFLEALIESTFYGNEVLDFAAS
HHHHHCCCCCCCHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHC
HESMFHLEHPAAQTIFSHLVRRFREMNDRDGHLDINSEISSIGMEDASNLAFDILFRLPV
CCHHEEECCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHCCCCHHHHHHHHHHEECCC
NETTRLTPAELEQHARRCLSHFLVAVKALVLEPLQKEKQKIIAQLQSATSTHEQERLSRE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEHNKQFRAMEQEVDESIRGILGE
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]