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The map label for this gene is mscL

Identifier: 21674066

GI number: 21674066

Start: 1170184

End: 1170639

Strand: Reverse

Name: mscL

Synonym: CT1243

Alternate gene names: 21674066

Gene position: 1170639-1170184 (Counterclockwise)

Preceding gene: 21674067

Following gene: 21674065

Centisome position: 54.32

GC content: 52.85

Gene sequence:

>456_bases
ATGCTGAAAGAGTTCAGAGAGTTTGCCCTCAAGGGCAATGTAGTTGATATGGCTGTTGGCATCATCATTGGTGGCGCCTT
TGGGGCGTTGGTCAATTCTCTGGTGAACGATCTTCTGATGCCGCCGCTTGGCCTTCTTTTGAAAGGGGTTGATTTTTCAA
ATCTTTTTGTCGTCCTGAAAGAGGGTACTCCACCAGGGCCCTATATCGCATTGGCTGACGCCAAAACCGCCGGCGCGGTC
ACGCTCAATTACGGCCTGTTCGTCAATGCGCTTATCGGCTTTCTGATCATGGCCTTTGCCGTGTTTCTCCTTGTCCGGTC
GATCAATAGGCTTCGCAGCCTGAGCGAAAAGTCAGCCGCTCCAGCCGTCGCACCTCAGACCAAAGAGTGCCCTTTCTGCT
TCTCGATTATCCCGCTCAAAGCTGTGCGGTGCCCGAATTGCACGTCGCAGTTGTAA

Upstream 100 bases:

>100_bases
ATACACTAATGTCCAATTAAGGGGGTTTATTTTTTGGCCTTCCCGGAATGTCGTATCAGTGTAACATGTACAATCGGATA
AACAACAAACAAAGACGACT

Downstream 100 bases:

>100_bases
AAAACGGATGAGTCGGGAGCTTCCTGGTCTGGTAACAAACGGCAGGTTTTTTTTGGTAGTTCTTCTATGGAGATCAACAG
CATTTTGTTTATGATTGGGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MLKEFREFALKGNVVDMAVGIIIGGAFGALVNSLVNDLLMPPLGLLLKGVDFSNLFVVLKEGTPPGPYIALADAKTAGAV
TLNYGLFVNALIGFLIMAFAVFLLVRSINRLRSLSEKSAAPAVAPQTKECPFCFSIIPLKAVRCPNCTSQL

Sequences:

>Translated_151_residues
MLKEFREFALKGNVVDMAVGIIIGGAFGALVNSLVNDLLMPPLGLLLKGVDFSNLFVVLKEGTPPGPYIALADAKTAGAV
TLNYGLFVNALIGFLIMAFAVFLLVRSINRLRSLSEKSAAPAVAPQTKECPFCFSIIPLKAVRCPNCTSQL
>Mature_151_residues
MLKEFREFALKGNVVDMAVGIIIGGAFGALVNSLVNDLLMPPLGLLLKGVDFSNLFVVLKEGTPPGPYIALADAKTAGAV
TLNYGLFVNALIGFLIMAFAVFLLVRSINRLRSLSEKSAAPAVAPQTKECPFCFSIIPLKAVRCPNCTSQL

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=131, Percent_Identity=47.3282442748092, Blast_Score=118, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_CHLTE (Q8KD14)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662131.1
- ProteinModelPortal:   Q8KD14
- SMR:   Q8KD14
- GeneID:   1006601
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1243
- NMPDR:   fig|194439.1.peg.1225
- TIGR:   CT1243
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- ProtClustDB:   PRK00567
- BioCyc:   CTEP194439:CT_1243-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 16057; Mature: 16057

Theoretical pI: Translated: 8.99; Mature: 8.99

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xe2a280c)-; HASH(0xe510010)-;

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFREFALKGNVVDMAVGIIIGGAFGALVNSLVNDLLMPPLGLLLKGVDFSNLFVVLK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHEEEEEE
EGTPPGPYIALADAKTAGAVTLNYGLFVNALIGFLIMAFAVFLLVRSINRLRSLSEKSAA
CCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAVAPQTKECPFCFSIIPLKAVRCPNCTSQL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH
>Mature Secondary Structure
MLKEFREFALKGNVVDMAVGIIIGGAFGALVNSLVNDLLMPPLGLLLKGVDFSNLFVVLK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHEEEEEE
EGTPPGPYIALADAKTAGAVTLNYGLFVNALIGFLIMAFAVFLLVRSINRLRSLSEKSAA
CCCCCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PAVAPQTKECPFCFSIIPLKAVRCPNCTSQL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12093901