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The map label for this gene is rpoN [H]

Identifier: 21674019

GI number: 21674019

Start: 1121810

End: 1123264

Strand: Reverse

Name: rpoN [H]

Synonym: CT1193

Alternate gene names: 21674019

Gene position: 1123264-1121810 (Counterclockwise)

Preceding gene: 21674020

Following gene: 21674018

Centisome position: 52.12

GC content: 57.87

Gene sequence:

>1455_bases
ATGGCGGAGATGAGGCTACAACAGCGCCAGACCGCCCAGTTGTCGGCGCAGCAGGTGATGACCAACCAGTTGTTGCAGCT
GCCGCTGATGCAGCTCGAGCAGCGGATTTACGACGAGGTTCAGGACAATCCCATGCTCGAACTGGTCGAGGAGCGTCGTC
AGGACGATGGAGCGGCTGGTTCCACTCAGGCGGCTACCGCTGATTCGGCGGAGATGTTCGATTCGGTCTCCCGCTTCGAG
CGTTCGTCGATGAAGGTTCGCGCCGATGGAGGGAACCGGGAGACCGTTTCTGCCGGCAGGACTAGCGGCTCGGCTTCGGG
CGGCGAGGAGCGTTTTTTCCAGGCGGTGCAGCACGACACGCTTCACGAACGGTTGTTGCGCGATCTCTCGCTCCAGGAGG
GGATTGGAGAGCGCGAGGTGCGCATCGCCGCCGAGATTCTCGGCAACCTCGACAGCGATGGCTATCTGACCGAACCGCTG
GAAGTGATCATCGATGGGCTTCGACAGTCAGATATCGATGCCAGCGAGGCAGATGTTCGCGAGATACAGCAGAAAATCTG
GTATCTCGATCCGCCCGGCGTGGCGGTGGCCAATCTTCGCGAACGCCTGCTGGTTGAACTTTCGGTGTACGAACACGAGC
ACGATCCCGAAGCGGTCGGCGTTGCGCGAACGATCCTCAATGAAGCGTTCGACGACTTCATGAACAAGCGCTTCGACCGG
CTGCTGAAAAAGCTGAATCTTCAAAAACGCCAGCTCGAAGCCGCTATCGATGTGATCACGTCGCTTGATCCGCATCCGGG
TGAAGCGTTTTTCGATGAGGGAGGGCACTACATTTCGCCCGATTTCATTGTTATCTACGAAAATGGCGCGTTGACCGCGG
TTTTGAACGACCGGAGCAGTCTGTCGGTCAGAGTGTCGGACGAGTATCGGGAGGTGCTTGCGAAGCGGAAGGTGCCAAAG
GAGGATCGGCAGTTCATGCGCCAGAAGCTCCAGCGGGCCAATGAATTTGTCACTGCCCTGCAAGTCCGGCGGCAGACGCT
GCTGAAGGTGATGGAGGCGCTGCTGGTGGCGCAGGCGAAGTTTTTCATCGACGGCCCGCGCTATCTTCAGCCGCTTGTCA
TGAAGACGATCGCCGAGCAGACGGGCTACGATATTTCAACCATCAGCCGAGCGGTGAACGGAAAATACGTTCAGACCCGT
TTCGGGGTGTTCGAACTGAAATATTTTTTCAGCGGTGCGGTATCGACCGACGAAGGCGAGGAGCTGTCGTCGAGGATCAT
CAAGCAGCAGCTTCGCGATCTGATCGAGGGGGAGAACCCGGTCGAGCCGCTCAGCGACGACCGGCTGGCGGAATTGCTGA
GCGGAAAAGGGGTGCAGATCGCCCGCCGCACGGTTGCAAAATACCGTGAACAAATGCAAATTCCAGTTGCAAGATTAAGG
AAAAAAATAGGTTAA

Upstream 100 bases:

>100_bases
TGTCAGGCTGATCGATTTCCTCGAAGCGCCACTTGTTGAAAAGTACGGACACGACTGGTACATGAGCTTGAAGGAATTTG
TCGCATCCATCTGAGGTATC

Downstream 100 bases:

>100_bases
TCATTATTTCGACTACGACTCCATGGGCTACGAAAAACCGCAGATACGATCGACTACCGTTATCGGCATCATCAGGGATG
GCAAGGCTGCGCTTGGCAGC

Product: RNA polymerase sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MAEMRLQQRQTAQLSAQQVMTNQLLQLPLMQLEQRIYDEVQDNPMLELVEERRQDDGAAGSTQAATADSAEMFDSVSRFE
RSSMKVRADGGNRETVSAGRTSGSASGGEERFFQAVQHDTLHERLLRDLSLQEGIGEREVRIAAEILGNLDSDGYLTEPL
EVIIDGLRQSDIDASEADVREIQQKIWYLDPPGVAVANLRERLLVELSVYEHEHDPEAVGVARTILNEAFDDFMNKRFDR
LLKKLNLQKRQLEAAIDVITSLDPHPGEAFFDEGGHYISPDFIVIYENGALTAVLNDRSSLSVRVSDEYREVLAKRKVPK
EDRQFMRQKLQRANEFVTALQVRRQTLLKVMEALLVAQAKFFIDGPRYLQPLVMKTIAEQTGYDISTISRAVNGKYVQTR
FGVFELKYFFSGAVSTDEGEELSSRIIKQQLRDLIEGENPVEPLSDDRLAELLSGKGVQIARRTVAKYREQMQIPVARLR
KKIG

Sequences:

>Translated_484_residues
MAEMRLQQRQTAQLSAQQVMTNQLLQLPLMQLEQRIYDEVQDNPMLELVEERRQDDGAAGSTQAATADSAEMFDSVSRFE
RSSMKVRADGGNRETVSAGRTSGSASGGEERFFQAVQHDTLHERLLRDLSLQEGIGEREVRIAAEILGNLDSDGYLTEPL
EVIIDGLRQSDIDASEADVREIQQKIWYLDPPGVAVANLRERLLVELSVYEHEHDPEAVGVARTILNEAFDDFMNKRFDR
LLKKLNLQKRQLEAAIDVITSLDPHPGEAFFDEGGHYISPDFIVIYENGALTAVLNDRSSLSVRVSDEYREVLAKRKVPK
EDRQFMRQKLQRANEFVTALQVRRQTLLKVMEALLVAQAKFFIDGPRYLQPLVMKTIAEQTGYDISTISRAVNGKYVQTR
FGVFELKYFFSGAVSTDEGEELSSRIIKQQLRDLIEGENPVEPLSDDRLAELLSGKGVQIARRTVAKYREQMQIPVARLR
KKIG
>Mature_483_residues
AEMRLQQRQTAQLSAQQVMTNQLLQLPLMQLEQRIYDEVQDNPMLELVEERRQDDGAAGSTQAATADSAEMFDSVSRFER
SSMKVRADGGNRETVSAGRTSGSASGGEERFFQAVQHDTLHERLLRDLSLQEGIGEREVRIAAEILGNLDSDGYLTEPLE
VIIDGLRQSDIDASEADVREIQQKIWYLDPPGVAVANLRERLLVELSVYEHEHDPEAVGVARTILNEAFDDFMNKRFDRL
LKKLNLQKRQLEAAIDVITSLDPHPGEAFFDEGGHYISPDFIVIYENGALTAVLNDRSSLSVRVSDEYREVLAKRKVPKE
DRQFMRQKLQRANEFVTALQVRRQTLLKVMEALLVAQAKFFIDGPRYLQPLVMKTIAEQTGYDISTISRAVNGKYVQTRF
GVFELKYFFSGAVSTDEGEELSSRIIKQQLRDLIEGENPVEPLSDDRLAELLSGKGVQIARRTVAKYREQMQIPVARLRK
KIG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of enzymes involved in arginine catabolism. The open complex (sigma-

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=481, Percent_Identity=34.0956340956341, Blast_Score=244, Evalue=1e-65,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 54835; Mature: 54704

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEMRLQQRQTAQLSAQQVMTNQLLQLPLMQLEQRIYDEVQDNPMLELVEERRQDDGAAG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
STQAATADSAEMFDSVSRFERSSMKVRADGGNRETVSAGRTSGSASGGEERFFQAVQHDT
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LHERLLRDLSLQEGIGEREVRIAAEILGNLDSDGYLTEPLEVIIDGLRQSDIDASEADVR
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
EIQQKIWYLDPPGVAVANLRERLLVELSVYEHEHDPEAVGVARTILNEAFDDFMNKRFDR
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLKKLNLQKRQLEAAIDVITSLDPHPGEAFFDEGGHYISPDFIVIYENGALTAVLNDRSS
HHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCCEEEEECCCEEEEEECCCCC
LSVRVSDEYREVLAKRKVPKEDRQFMRQKLQRANEFVTALQVRRQTLLKVMEALLVAQAK
EEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFIDGPRYLQPLVMKTIAEQTGYDISTISRAVNGKYVQTRFGVFELKYFFSGAVSTDEGE
HHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHH
ELSSRIIKQQLRDLIEGENPVEPLSDDRLAELLSGKGVQIARRTVAKYREQMQIPVARLR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
KKIG
HHCC
>Mature Secondary Structure 
AEMRLQQRQTAQLSAQQVMTNQLLQLPLMQLEQRIYDEVQDNPMLELVEERRQDDGAAG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
STQAATADSAEMFDSVSRFERSSMKVRADGGNRETVSAGRTSGSASGGEERFFQAVQHDT
CCCCCCCHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LHERLLRDLSLQEGIGEREVRIAAEILGNLDSDGYLTEPLEVIIDGLRQSDIDASEADVR
HHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
EIQQKIWYLDPPGVAVANLRERLLVELSVYEHEHDPEAVGVARTILNEAFDDFMNKRFDR
HHHHHHEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLKKLNLQKRQLEAAIDVITSLDPHPGEAFFDEGGHYISPDFIVIYENGALTAVLNDRSS
HHHHHCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCEECCCEEEEECCCEEEEEECCCCC
LSVRVSDEYREVLAKRKVPKEDRQFMRQKLQRANEFVTALQVRRQTLLKVMEALLVAQAK
EEEEECHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFIDGPRYLQPLVMKTIAEQTGYDISTISRAVNGKYVQTRFGVFELKYFFSGAVSTDEGE
HHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCHH
ELSSRIIKQQLRDLIEGENPVEPLSDDRLAELLSGKGVQIARRTVAKYREQMQIPVARLR
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
KKIG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2203540; 8444818; 8025669; 7876255; 9278503 [H]