Click here to switch to the map view.

The map label for this gene is hslU

Identifier: 21674017

GI number: 21674017

Start: 1119684

End: 1121159

Strand: Reverse

Name: hslU

Synonym: CT1191

Alternate gene names: 21674017

Gene position: 1121159-1119684 (Counterclockwise)

Preceding gene: 21674018

Following gene: 21674016

Centisome position: 52.03

GC content: 57.05

Gene sequence:

>1476_bases
ATGATCCAGCCGGATGAGCCTCAGGATTTTCCGGTCAAACTCATCGACAAGGAGCAGCTCACGCCGACACAGATCGTCGA
GCAGCTCGACAAGTACATCATCGGCCAGAAAGACGCCAAGAGATCGGTAGCCATCGCGCTGCGTAACCGGCTCCGCCGCC
AGAATGTGAGCGAGGAGCTGCGTGACGAGATCATGCCGAACAACATCATCATGATTGGCCCGACCGGCGTTGGCAAGACC
GAAATAGCCCGGCGTCTGGCCAAGCTTGCCAAAGCTCCCTTTGTTAAGGTCGAGGCCTCAAAATTCACCGAGGTTGGCTA
CGTTGGTCGCGATGTCGAGTCGATGATCCGTGACCTGGTCGAGCAGGCGGTGGCGATGGTGCGCAGCGAAAAAACGGAGG
AGGTTCGCGAAAAAGCGGCGCTGCTGGTCGAGGAGCGTCTGCTCGACATTCTTCTGCCTCCCGTGAGCGGGCTCGAAGAG
TCGGAACACGTTGGCGACGAAGAGGAGGCCGTGGTTGTTGAAGGTGACGCCGAGGTGGTGGTGGAGAAGAACCTCGAACG
TGAAATCAACCGCAAAAGCCGCCAGAAGATGCGTGAGCGCCTGCGCGACGGCAGAATGGAGGATCGCCAGATAGAGCTGG
AGGTCAGTAGCGACGGTCAGGGGGGCATGATGCAGATATTTGGTCCACTCGGGCAGATGGAGGAGATTGGCAACATCATG
CAAGATCTGATGAGCGGTATGCCGAAAAAACGCAAAAAGCGCCGCATGACCATCGCCGAAGCCCGCAAGTATCTGGAGCA
GGAGGAGGTGCAGAAGCTCATTGACATGGATGCTGTGGTCAAGGAGGCGCTTCGCAAGGTTGAGGATTCTGGCATCGTGT
TCATCGACGAGATCGATAAAATTGCTGCTCCAACCACCGGCGCTGGCGGCAAGGGCCCTGATGTCAGCCGTGAAGGCGTG
CAGCGCGACCTGCTGCCCATCGTCGAGGGCACGGCGGTCTCCACCAAGTATGGCGTCGTCAAAACCGATCACGTGCTCTT
TATTGCTTCGGGAGCGTTCCATGTGGCGAGGCCGTCCGACCTTATACCTGAACTTCAGGGTCGTTTTCCGATCAGGGTCG
AGCTGAAAAGTCTTACCGAAGAGGACTTTTTCCTAATCCTGACCCAGCCGCGCAATGCGCTGATCAAGCAGTACCGCGCC
ATGCTCAAGACCGAACAGATCGATCTCGAGTTCACCGAGGAGGCGATTCGCGAGATTGCACGGACGGCGGCGAAAGTCAA
CGAGACGGTTGAAAATATCGGTGCTCGTCGCCTGCACACCATTCTGACCAACCTGCTCGAAGAGCTGATGTTTGGGATTC
CTGAAATGGTCATGGATGGCACCATCGACAGGAACATAGTTATCGACGACAACCAGGTGCGCGAAAAGCTGGGCAAGCTA
GTCGCCGACCGGGATCTGAGCCAGTATATCCTCTAA

Upstream 100 bases:

>100_bases
GTCATCGAAACTCTGTGACCCAGCGCATCTGCGTCAAGTCGCAGCAGGTCCATTTTCATGAAATTCCCAACGACACTCAA
TGAACAACGATATTCAGGAT

Downstream 100 bases:

>100_bases
GCCAAAAAGCCGGAGCAACCATGATGAGCGCAACCTCCCTTCTGGTCATGAACGGGCCGAATCTTTCACGTCTCGGAAAG
CGTGAACCCGAAGTTTATGG

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MIQPDEPQDFPVKLIDKEQLTPTQIVEQLDKYIIGQKDAKRSVAIALRNRLRRQNVSEELRDEIMPNNIIMIGPTGVGKT
EIARRLAKLAKAPFVKVEASKFTEVGYVGRDVESMIRDLVEQAVAMVRSEKTEEVREKAALLVEERLLDILLPPVSGLEE
SEHVGDEEEAVVVEGDAEVVVEKNLEREINRKSRQKMRERLRDGRMEDRQIELEVSSDGQGGMMQIFGPLGQMEEIGNIM
QDLMSGMPKKRKKRRMTIAEARKYLEQEEVQKLIDMDAVVKEALRKVEDSGIVFIDEIDKIAAPTTGAGGKGPDVSREGV
QRDLLPIVEGTAVSTKYGVVKTDHVLFIASGAFHVARPSDLIPELQGRFPIRVELKSLTEEDFFLILTQPRNALIKQYRA
MLKTEQIDLEFTEEAIREIARTAAKVNETVENIGARRLHTILTNLLEELMFGIPEMVMDGTIDRNIVIDDNQVREKLGKL
VADRDLSQYIL

Sequences:

>Translated_491_residues
MIQPDEPQDFPVKLIDKEQLTPTQIVEQLDKYIIGQKDAKRSVAIALRNRLRRQNVSEELRDEIMPNNIIMIGPTGVGKT
EIARRLAKLAKAPFVKVEASKFTEVGYVGRDVESMIRDLVEQAVAMVRSEKTEEVREKAALLVEERLLDILLPPVSGLEE
SEHVGDEEEAVVVEGDAEVVVEKNLEREINRKSRQKMRERLRDGRMEDRQIELEVSSDGQGGMMQIFGPLGQMEEIGNIM
QDLMSGMPKKRKKRRMTIAEARKYLEQEEVQKLIDMDAVVKEALRKVEDSGIVFIDEIDKIAAPTTGAGGKGPDVSREGV
QRDLLPIVEGTAVSTKYGVVKTDHVLFIASGAFHVARPSDLIPELQGRFPIRVELKSLTEEDFFLILTQPRNALIKQYRA
MLKTEQIDLEFTEEAIREIARTAAKVNETVENIGARRLHTILTNLLEELMFGIPEMVMDGTIDRNIVIDDNQVREKLGKL
VADRDLSQYIL
>Mature_491_residues
MIQPDEPQDFPVKLIDKEQLTPTQIVEQLDKYIIGQKDAKRSVAIALRNRLRRQNVSEELRDEIMPNNIIMIGPTGVGKT
EIARRLAKLAKAPFVKVEASKFTEVGYVGRDVESMIRDLVEQAVAMVRSEKTEEVREKAALLVEERLLDILLPPVSGLEE
SEHVGDEEEAVVVEGDAEVVVEKNLEREINRKSRQKMRERLRDGRMEDRQIELEVSSDGQGGMMQIFGPLGQMEEIGNIM
QDLMSGMPKKRKKRRMTIAEARKYLEQEEVQKLIDMDAVVKEALRKVEDSGIVFIDEIDKIAAPTTGAGGKGPDVSREGV
QRDLLPIVEGTAVSTKYGVVKTDHVLFIASGAFHVARPSDLIPELQGRFPIRVELKSLTEEDFFLILTQPRNALIKQYRA
MLKTEQIDLEFTEEAIREIARTAAKVNETVENIGARRLHTILTNLLEELMFGIPEMVMDGTIDRNIVIDDNQVREKLGKL
VADRDLSQYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=94, Percent_Identity=43.6170212765958, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790366, Length=474, Percent_Identity=51.0548523206751, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1786642, Length=259, Percent_Identity=31.2741312741313, Blast_Score=98, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6319704, Length=108, Percent_Identity=37.037037037037, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24648289, Length=251, Percent_Identity=30.2788844621514, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648291, Length=251, Percent_Identity=30.2788844621514, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_CHLTE (Q8KD63)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_662082.1
- ProteinModelPortal:   Q8KD63
- SMR:   Q8KD63
- GeneID:   1006553
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT1191
- NMPDR:   fig|194439.1.peg.1176
- TIGR:   CT1191
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   CTEP194439:CT_1191-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 55515; Mature: 55515

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: BINDING 34-34 BINDING 296-296 BINDING 364-364 BINDING 436-436

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQPDEPQDFPVKLIDKEQLTPTQIVEQLDKYIIGQKDAKRSVAIALRNRLRRQNVSEEL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
RDEIMPNNIIMIGPTGVGKTEIARRLAKLAKAPFVKVEASKFTEVGYVGRDVESMIRDLV
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCCHHHHHHHHH
EQAVAMVRSEKTEEVREKAALLVEERLLDILLPPVSGLEESEHVGDEEEAVVVEGDAEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEECCCCEE
VEKNLEREINRKSRQKMRERLRDGRMEDRQIELEVSSDGQGGMMQIFGPLGQMEEIGNIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHH
QDLMSGMPKKRKKRRMTIAEARKYLEQEEVQKLIDMDAVVKEALRKVEDSGIVFIDEIDK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
IAAPTTGAGGKGPDVSREGVQRDLLPIVEGTAVSTKYGVVKTDHVLFIASGAFHVARPSD
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEECCEEEEECCCEECCCCHH
LIPELQGRFPIRVELKSLTEEDFFLILTQPRNALIKQYRAMLKTEQIDLEFTEEAIREIA
HHHHHCCCCCEEEEHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RTAAKVNETVENIGARRLHTILTNLLEELMFGIPEMVMDGTIDRNIVIDDNQVREKLGKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEECCHHHHHHHHHH
VADRDLSQYIL
HHHCCHHHHCC
>Mature Secondary Structure
MIQPDEPQDFPVKLIDKEQLTPTQIVEQLDKYIIGQKDAKRSVAIALRNRLRRQNVSEEL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHH
RDEIMPNNIIMIGPTGVGKTEIARRLAKLAKAPFVKVEASKFTEVGYVGRDVESMIRDLV
HHHHCCCCEEEECCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCCCCHHHHHHHHH
EQAVAMVRSEKTEEVREKAALLVEERLLDILLPPVSGLEESEHVGDEEEAVVVEGDAEVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCEEEEECCCCEE
VEKNLEREINRKSRQKMRERLRDGRMEDRQIELEVSSDGQGGMMQIFGPLGQMEEIGNIM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHH
QDLMSGMPKKRKKRRMTIAEARKYLEQEEVQKLIDMDAVVKEALRKVEDSGIVFIDEIDK
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHH
IAAPTTGAGGKGPDVSREGVQRDLLPIVEGTAVSTKYGVVKTDHVLFIASGAFHVARPSD
HCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEEECCEEEEECCCEECCCCHH
LIPELQGRFPIRVELKSLTEEDFFLILTQPRNALIKQYRAMLKTEQIDLEFTEEAIREIA
HHHHHCCCCCEEEEHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
RTAAKVNETVENIGARRLHTILTNLLEELMFGIPEMVMDGTIDRNIVIDDNQVREKLGKL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCEEECCHHHHHHHHHH
VADRDLSQYIL
HHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901