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The map label for this gene is priA [H]

Identifier: 21674013

GI number: 21674013

Start: 1115229

End: 1117664

Strand: Direct

Name: priA [H]

Synonym: CT1187

Alternate gene names: 21674013

Gene position: 1115229-1117664 (Clockwise)

Preceding gene: 21674011

Following gene: 21674014

Centisome position: 51.75

GC content: 61.62

Gene sequence:

>2436_bases
TTGCTGATGTATGCGATTGTCTATGTAGAAAGAATCTATCGTGACGAGCCTTTTCGGCTGAGCATGCCGGAGGGGCTCAA
AACGACGATTCAGCCGGGCTGCCAGGTGCTGCTGAACCTTGCCCGCCACAAAGCGTCGGCTTACACGGGCTATGTCTGGT
CGCTGGAAAAGGCGGGCGAGGGCGACATCGAGGGCGAAGTGCTCGACCTGCTCAACAGCGGTGTTCCGGTGCTGACGCCG
GTAATGCTGAAACTCGCTTTGTGGATTGCCGACTACTACGCAGCCCTCCCCATCGACTGCCTCTCCACCACCCTTCCGGC
TCCGCTCAGGAGTACGGTCGATGACGTGGTGGAGCTGGCCGGGTTCCAGCTCGAAAGCCCGGAATTACGAGTCAAAAGCA
CGGGGTTGCGCCGGAGCATCCTGAAAGCGCTGGCGACTGAAAAACGGCTCACCGTGCGCCAGCTCCGCAAACGCCTCGGT
CGCAGGGAGCTGTACAGCGCGATCGCGGAACTCGAACGCGCCGGGCTGGTCAACGTCCGCAAGAGCTTCGTGGAAACGAA
GCCCCGCACGGTCACCACATGGAAACTGGCGAAGACGCTGCCCGATGAACCGGAAAAACTCATTGCACGTTCGCCAAAGA
AACGCGAGGCGTTCGAGCTGCTCGCCTCGCGCCCGGAGCAGCTATTCCGCGCCGGAGAGGGCGGTATTTCACGAACGGTA
TTCAGCGGACTTGTCACTCTCGGCCTCGCCGAAAAGATCGAAACCGCAGCGCCCTCTGGCGAAAGCCTGCGCTTCGACGA
GCCGCAAAAGGAGATTCATTCCCTCAGTCCGCACCAGCAGCAGGCGCTTGACGCCTTGACCAAAGCGCTCTACGAACAGC
AATTCCGGACTTTCCTTTTGCACGGCGTAACTGGCAGCGGCAAAACGCTGGTTTACATCGAGTTGCTCCGCCGTGTGCTC
GCCGAGGGCAAAACCGCCATTGTTTTGGTGCCGGAAATCTCACTCACACCACAGACCGCCGCCCGGTTCCGCCACTATTT
CGGCGACGATATCCAGATCATGCACAGCGCCATGAGCGACCGCGAAAAGTACGATGCCTGGCAGCGGCTGCGACAGGGCA
AGGCACGCATCGCGCTCGGCGCTCGCTCGACCATTTTCGCGCCGCTGGAGAATGTCGGTGCGATCATCGTGGACGAGGAG
CACGACGCCGCCTACAAGCAGGATCGCACGCCTCGCTACCACGCCCGCGACACGGCAGTGATGCGCGCCATGTTCGAAAA
CGCACTCTGCGTGCTCGGCTCGGCAACGCCATCGTTCGAGTCCTACCGGAACGCGCTGGAGGGCAAGTACACACTACTCG
AACTGCCCGAACGCATCGACAACGCCCGAATGCCCTCCATCGAGCTGGTCTGGATGCCCGGCAGCCAGCGGGTCACACCG
TCGATCTCGGGCGCGCTCTACGACGCCATCCGCGAAAGGCTCAAGCGCGACGAGCAGGTGATCCTCCTGCAAAATCGCCG
GGGCTTCGCAGGCAGCCTGCTCTGCCTCGACTGCGGCCACACGCCGCAATGCCGCCACTGCAACATTCCGCTGGTCTATC
ACGCCAGCGATCGGAGCCTGCGCTGCCACTACTGCGGTCACATTGAACCGTTCCGCCAAACGTGCCCTGCGTGCAAATCG
GAAAATCTCTTCTACAAAAGCAGTGGCACTGAGCGCATCGAGGAGGAACTTGGAGAGCTGTTTCCCGAAGAGAAGATTCT
GCGGATGGACATCGACACCACCTCGACCAAGGACGCCCATGCGTCGATGCTGACTGCTTTCCGCGAAAAGCGCGCCCGCA
TCCTGCTCGGCACGCAGATGGTGGCCAAGGGCCTCGACTTTCCAGAGGTAACGCTGGTCGGGGTGCTGATGGCGGACATC
GGCCTGAACCTGCCGGACTTCAGAGCCGCCGAACGCATCTACTCGCTCCTGATGCAGGTTGCCGGGCGGGCGGGCCGCTC
GTCGATGCCCGGCGAGGTGCTGCTTCAGCTCTACAACCGCGACAACGAGCTGTTCCAGCACGTCATCCGGGCTGACTACC
GCCACTTTTTCGAGGCGGAGATGGCCACGCGCCGCGAACTCGCCTACCCGCCGTTCACCCGCCTCATCAAGTTCGAGTGT
TCGTCGCCATCGGAAGCGGTGGCCGAAAAAGGGGCGGTGGCACTGCGGAAGCATCTGCGACCGCTGGTGCCGGAAGCGTT
CGGCACGATCCTCGGCCCCGCCCCGGCGGGCATCAGCAAAATAAAGGGGCGCTACCGCAGCCAGCTCATCATCAAGCTCA
CCGGCATCAAGCTCTCAGCCGCCCTGCTTCGGCAAGTACAATACGAAACCCTCAGCGCTTTTCGGGGAGAAAATCTTGTC
ATCACGGTCGATGTCGATCCGCAACATCTTCTGTAA

Upstream 100 bases:

>100_bases
AAGCCAGTTTGAACCACTGGCTTTTTTTCATTATGAATGATGAACTTTGAATGAGCAATCTTTTTCCTGCCCTTGTTGCC
GTTTTCTTTCAAACTCCTGA

Downstream 100 bases:

>100_bases
TGCGCTTTTTTCGTAAGTTTGCGATACATTCAAATTATAGCGCAAGACGAACAATGCCCCACGGAAAAGCAACCTTGAAC
CCTTGTTCAGCAAGTCATGA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 811; Mature: 811

Protein sequence:

>811_residues
MLMYAIVYVERIYRDEPFRLSMPEGLKTTIQPGCQVLLNLARHKASAYTGYVWSLEKAGEGDIEGEVLDLLNSGVPVLTP
VMLKLALWIADYYAALPIDCLSTTLPAPLRSTVDDVVELAGFQLESPELRVKSTGLRRSILKALATEKRLTVRQLRKRLG
RRELYSAIAELERAGLVNVRKSFVETKPRTVTTWKLAKTLPDEPEKLIARSPKKREAFELLASRPEQLFRAGEGGISRTV
FSGLVTLGLAEKIETAAPSGESLRFDEPQKEIHSLSPHQQQALDALTKALYEQQFRTFLLHGVTGSGKTLVYIELLRRVL
AEGKTAIVLVPEISLTPQTAARFRHYFGDDIQIMHSAMSDREKYDAWQRLRQGKARIALGARSTIFAPLENVGAIIVDEE
HDAAYKQDRTPRYHARDTAVMRAMFENALCVLGSATPSFESYRNALEGKYTLLELPERIDNARMPSIELVWMPGSQRVTP
SISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLDCGHTPQCRHCNIPLVYHASDRSLRCHYCGHIEPFRQTCPACKS
ENLFYKSSGTERIEEELGELFPEEKILRMDIDTTSTKDAHASMLTAFREKRARILLGTQMVAKGLDFPEVTLVGVLMADI
GLNLPDFRAAERIYSLLMQVAGRAGRSSMPGEVLLQLYNRDNELFQHVIRADYRHFFEAEMATRRELAYPPFTRLIKFEC
SSPSEAVAEKGAVALRKHLRPLVPEAFGTILGPAPAGISKIKGRYRSQLIIKLTGIKLSAALLRQVQYETLSAFRGENLV
ITVDVDPQHLL

Sequences:

>Translated_811_residues
MLMYAIVYVERIYRDEPFRLSMPEGLKTTIQPGCQVLLNLARHKASAYTGYVWSLEKAGEGDIEGEVLDLLNSGVPVLTP
VMLKLALWIADYYAALPIDCLSTTLPAPLRSTVDDVVELAGFQLESPELRVKSTGLRRSILKALATEKRLTVRQLRKRLG
RRELYSAIAELERAGLVNVRKSFVETKPRTVTTWKLAKTLPDEPEKLIARSPKKREAFELLASRPEQLFRAGEGGISRTV
FSGLVTLGLAEKIETAAPSGESLRFDEPQKEIHSLSPHQQQALDALTKALYEQQFRTFLLHGVTGSGKTLVYIELLRRVL
AEGKTAIVLVPEISLTPQTAARFRHYFGDDIQIMHSAMSDREKYDAWQRLRQGKARIALGARSTIFAPLENVGAIIVDEE
HDAAYKQDRTPRYHARDTAVMRAMFENALCVLGSATPSFESYRNALEGKYTLLELPERIDNARMPSIELVWMPGSQRVTP
SISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLDCGHTPQCRHCNIPLVYHASDRSLRCHYCGHIEPFRQTCPACKS
ENLFYKSSGTERIEEELGELFPEEKILRMDIDTTSTKDAHASMLTAFREKRARILLGTQMVAKGLDFPEVTLVGVLMADI
GLNLPDFRAAERIYSLLMQVAGRAGRSSMPGEVLLQLYNRDNELFQHVIRADYRHFFEAEMATRRELAYPPFTRLIKFEC
SSPSEAVAEKGAVALRKHLRPLVPEAFGTILGPAPAGISKIKGRYRSQLIIKLTGIKLSAALLRQVQYETLSAFRGENLV
ITVDVDPQHLL
>Mature_811_residues
MLMYAIVYVERIYRDEPFRLSMPEGLKTTIQPGCQVLLNLARHKASAYTGYVWSLEKAGEGDIEGEVLDLLNSGVPVLTP
VMLKLALWIADYYAALPIDCLSTTLPAPLRSTVDDVVELAGFQLESPELRVKSTGLRRSILKALATEKRLTVRQLRKRLG
RRELYSAIAELERAGLVNVRKSFVETKPRTVTTWKLAKTLPDEPEKLIARSPKKREAFELLASRPEQLFRAGEGGISRTV
FSGLVTLGLAEKIETAAPSGESLRFDEPQKEIHSLSPHQQQALDALTKALYEQQFRTFLLHGVTGSGKTLVYIELLRRVL
AEGKTAIVLVPEISLTPQTAARFRHYFGDDIQIMHSAMSDREKYDAWQRLRQGKARIALGARSTIFAPLENVGAIIVDEE
HDAAYKQDRTPRYHARDTAVMRAMFENALCVLGSATPSFESYRNALEGKYTLLELPERIDNARMPSIELVWMPGSQRVTP
SISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLDCGHTPQCRHCNIPLVYHASDRSLRCHYCGHIEPFRQTCPACKS
ENLFYKSSGTERIEEELGELFPEEKILRMDIDTTSTKDAHASMLTAFREKRARILLGTQMVAKGLDFPEVTLVGVLMADI
GLNLPDFRAAERIYSLLMQVAGRAGRSSMPGEVLLQLYNRDNELFQHVIRADYRHFFEAEMATRRELAYPPFTRLIKFEC
SSPSEAVAEKGAVALRKHLRPLVPEAFGTILGPAPAGISKIKGRYRSQLIIKLTGIKLSAALLRQVQYETLSAFRGENLV
ITVDVDPQHLL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=579, Percent_Identity=40.7599309153713, Blast_Score=394, Evalue=1e-110,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 91103; Mature: 91103

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMYAIVYVERIYRDEPFRLSMPEGLKTTIQPGCQVLLNLARHKASAYTGYVWSLEKAGE
CHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GDIEGEVLDLLNSGVPVLTPVMLKLALWIADYYAALPIDCLSTTLPAPLRSTVDDVVELA
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
GFQLESPELRVKSTGLRRSILKALATEKRLTVRQLRKRLGRRELYSAIAELERAGLVNVR
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KSFVETKPRTVTTWKLAKTLPDEPEKLIARSPKKREAFELLASRPEQLFRAGEGGISRTV
HHHHHCCCCEEEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCHHHHHHCCCCCHHHHH
FSGLVTLGLAEKIETAAPSGESLRFDEPQKEIHSLSPHQQQALDALTKALYEQQFRTFLL
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
HGVTGSGKTLVYIELLRRVLAEGKTAIVLVPEISLTPQTAARFRHYFGDDIQIMHSAMSD
HCCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
REKYDAWQRLRQGKARIALGARSTIFAPLENVGAIIVDEEHDAAYKQDRTPRYHARDTAV
HHHHHHHHHHHCCCCEEEECCCHHHHHCHHHCCEEEEECCCCHHHHCCCCCCCHHHHHHH
MRAMFENALCVLGSATPSFESYRNALEGKYTLLELPERIDNARMPSIELVWMPGSQRVTP
HHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLDCGHTPQCRHCNIPLVYHASDRSL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCE
RCHYCGHIEPFRQTCPACKSENLFYKSSGTERIEEELGELFPEEKILRMDIDTTSTKDAH
EEEECCCCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
ASMLTAFREKRARILLGTQMVAKGLDFPEVTLVGVLMADIGLNLPDFRAAERIYSLLMQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AGRAGRSSMPGEVLLQLYNRDNELFQHVIRADYRHFFEAEMATRRELAYPPFTRLIKFEC
HCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEC
SSPSEAVAEKGAVALRKHLRPLVPEAFGTILGPAPAGISKIKGRYRSQLIIKLTGIKLSA
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHCEEEEEEECCHHHH
ALLRQVQYETLSAFRGENLVITVDVDPQHLL
HHHHHHHHHHHHHHCCCCEEEEEECCHHHCC
>Mature Secondary Structure
MLMYAIVYVERIYRDEPFRLSMPEGLKTTIQPGCQVLLNLARHKASAYTGYVWSLEKAGE
CHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCC
GDIEGEVLDLLNSGVPVLTPVMLKLALWIADYYAALPIDCLSTTLPAPLRSTVDDVVELA
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHH
GFQLESPELRVKSTGLRRSILKALATEKRLTVRQLRKRLGRRELYSAIAELERAGLVNVR
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
KSFVETKPRTVTTWKLAKTLPDEPEKLIARSPKKREAFELLASRPEQLFRAGEGGISRTV
HHHHHCCCCEEEEEHHHHCCCCCHHHHHHCCCCHHHHHHHHHHCHHHHHHCCCCCHHHHH
FSGLVTLGLAEKIETAAPSGESLRFDEPQKEIHSLSPHQQQALDALTKALYEQQFRTFLL
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
HGVTGSGKTLVYIELLRRVLAEGKTAIVLVPEISLTPQTAARFRHYFGDDIQIMHSAMSD
HCCCCCCCEEHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
REKYDAWQRLRQGKARIALGARSTIFAPLENVGAIIVDEEHDAAYKQDRTPRYHARDTAV
HHHHHHHHHHHCCCCEEEECCCHHHHHCHHHCCEEEEECCCCHHHHCCCCCCCHHHHHHH
MRAMFENALCVLGSATPSFESYRNALEGKYTLLELPERIDNARMPSIELVWMPGSQRVTP
HHHHHHCCCEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCC
SISGALYDAIRERLKRDEQVILLQNRRGFAGSLLCLDCGHTPQCRHCNIPLVYHASDRSL
CHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCEEEECCCCCE
RCHYCGHIEPFRQTCPACKSENLFYKSSGTERIEEELGELFPEEKILRMDIDTTSTKDAH
EEEECCCCCHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCHHH
ASMLTAFREKRARILLGTQMVAKGLDFPEVTLVGVLMADIGLNLPDFRAAERIYSLLMQV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
AGRAGRSSMPGEVLLQLYNRDNELFQHVIRADYRHFFEAEMATRRELAYPPFTRLIKFEC
HCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEC
SSPSEAVAEKGAVALRKHLRPLVPEAFGTILGPAPAGISKIKGRYRSQLIIKLTGIKLSA
CCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCHHHHHHHHHCEEEEEEECCHHHH
ALLRQVQYETLSAFRGENLVITVDVDPQHLL
HHHHHHHHHHHHHHCCCCEEEEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]