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The map label for this gene is cca [H]

Identifier: 21673818

GI number: 21673818

Start: 938566

End: 939993

Strand: Reverse

Name: cca [H]

Synonym: CT0990

Alternate gene names: 21673818

Gene position: 939993-938566 (Counterclockwise)

Preceding gene: 21673819

Following gene: 21673817

Centisome position: 43.62

GC content: 59.8

Gene sequence:

>1428_bases
ATGCTGACAAGAACACACGAAGCCATTCCTGAAATCATGTACAGAATCGGAGACCTCGCCGACGAGACGGGACTCCCCTG
CTATATCGTGGGCGGCTACGTCCGCGACCTCATCATGCAGCGTCCCTGCACCGATATCGATATTATGGTCATCGGCGAGC
CAGTGCCCTTCGCCCACGCCCTTGCCGAAAAGCTCGGGGGGCGGAACTTCGTGCTCTTCGAGCGATTCCGCACCGCGCAG
CTCGAACTGGATGACCCGAAAGCAGGAACGTTCAAACTTGAAATCGTCGGCGCACGCAAGGAGAGCTACAACCCCGAATC
GCGCAAGCCGATCACCAGCATCGGCACGCTGGAGGACGACCTGTCACGCCGGGACTTCACGATAAACGCCCTCGCCTTGC
GACTGAACCGCAAAGAGCGTGGCGAAGTGGTCGATTTGTTCGACGGGCAGCGACACATCCGCGAAAAACTGCTCAAGACG
CCGCTCGATCCGGAACAAACGTTCTCCGACGACCCGCTCCGCATGATGCGCGCCGCCCGCTTCGCCTGCCAGCTCGACTT
CCAGCTTGACGAAGCGACTCTTACAGCGATGAGCACCATGAGCAGCCGGATCCAGATCGTCTCGCGCGAGCGCGTCAGCC
ACGAGTTTTTCAAGATCATGGAGGCGCGAAAACCCTCGATAGGCTTGAAAATTCTTTACTCGACCGGCCTGTTGAAGGAG
ATCATCCCCGAGCTGACCGTCATGGCGGGCATCGAGCAGGTGGACGGCCTTGGGCACAAAGATACGCTGTTCCACACGTT
TCAGGTGGTGGACAACCTCGCGGAGCACTCCGACAAGCTCTGGCTGCGGGTCAGCGCCCTCTTCCACGACATCGCCAAGC
CAGTCACAAAGCGCTTTCATCCTGGCTCGGGCTGGACCTTCCACGGCCATGAAGCTGTGGGCGTCAAAATCGTGGCGCGC
ATCTTCCGCGCGATGAAGTGGCCGCTGGAGCCGATGGAGTACGTGCAGAAGATGGTGCGGCTGCACCATCGCCCGATTCC
GCTCTCGAAGGAGGAGATCACCGACTCGGCGGTGCGCCGCCTGATGTTCGACGCCGGACCAGACCTCGACGACCTGATGA
CACTCTGCCGCGCAGACGTGACCAGCAAGAACCCGCGCAAGGTGCAGCGCATCATGAAGAACTTCAGCAACGTCGAAGCG
AAAATCGCCGAGGTTGGCGAAAAGGATCTGCTCGCCAAATGGCGTCCACCGGTCAGCGGCACCGACATCATGGAGCTGCT
CAACCTCCCGCAGGGGCGGACGGTCGGCATCATCAAATCGCGTATGGAGAATGCCATCATCGACGGCGACATTCCCTACG
ACCGGCAGGCTGCGCTTGATTACATTCAGCAAGTGTACCGCGAAATGCAGGAAAAAGGCGAAGCATGA

Upstream 100 bases:

>100_bases
CGCGCGTTGATGGTGGCCGGAAAAATCACCGATGAAATCCGGAATAATACGGCAGACCTTGACTGAATCCCGACCAGACG
AATACGATACCGACGACCGA

Downstream 100 bases:

>100_bases
ACGCTTTTTCGCTCTTCTCCTTGCAGGCCATCTTCTCCATGCCTTTGAAAGGCCAAGAACTATATTCATTACTACCGGAA
GCTTAAAACCAAAACCGAAC

Product: polyA polymerase family protein

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 475; Mature: 475

Protein sequence:

>475_residues
MLTRTHEAIPEIMYRIGDLADETGLPCYIVGGYVRDLIMQRPCTDIDIMVIGEPVPFAHALAEKLGGRNFVLFERFRTAQ
LELDDPKAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNRKERGEVVDLFDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFQLDEATLTAMSTMSSRIQIVSRERVSHEFFKIMEARKPSIGLKILYSTGLLKE
IIPELTVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHPGSGWTFHGHEAVGVKIVAR
IFRAMKWPLEPMEYVQKMVRLHHRPIPLSKEEITDSAVRRLMFDAGPDLDDLMTLCRADVTSKNPRKVQRIMKNFSNVEA
KIAEVGEKDLLAKWRPPVSGTDIMELLNLPQGRTVGIIKSRMENAIIDGDIPYDRQAALDYIQQVYREMQEKGEA

Sequences:

>Translated_475_residues
MLTRTHEAIPEIMYRIGDLADETGLPCYIVGGYVRDLIMQRPCTDIDIMVIGEPVPFAHALAEKLGGRNFVLFERFRTAQ
LELDDPKAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNRKERGEVVDLFDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFQLDEATLTAMSTMSSRIQIVSRERVSHEFFKIMEARKPSIGLKILYSTGLLKE
IIPELTVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHPGSGWTFHGHEAVGVKIVAR
IFRAMKWPLEPMEYVQKMVRLHHRPIPLSKEEITDSAVRRLMFDAGPDLDDLMTLCRADVTSKNPRKVQRIMKNFSNVEA
KIAEVGEKDLLAKWRPPVSGTDIMELLNLPQGRTVGIIKSRMENAIIDGDIPYDRQAALDYIQQVYREMQEKGEA
>Mature_475_residues
MLTRTHEAIPEIMYRIGDLADETGLPCYIVGGYVRDLIMQRPCTDIDIMVIGEPVPFAHALAEKLGGRNFVLFERFRTAQ
LELDDPKAGTFKLEIVGARKESYNPESRKPITSIGTLEDDLSRRDFTINALALRLNRKERGEVVDLFDGQRHIREKLLKT
PLDPEQTFSDDPLRMMRAARFACQLDFQLDEATLTAMSTMSSRIQIVSRERVSHEFFKIMEARKPSIGLKILYSTGLLKE
IIPELTVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFHPGSGWTFHGHEAVGVKIVAR
IFRAMKWPLEPMEYVQKMVRLHHRPIPLSKEEITDSAVRRLMFDAGPDLDDLMTLCRADVTSKNPRKVQRIMKNFSNVEA
KIAEVGEKDLLAKWRPPVSGTDIMELLNLPQGRTVGIIKSRMENAIIDGDIPYDRQAALDYIQQVYREMQEKGEA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=327, Percent_Identity=29.9694189602446, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081691, Length=330, Percent_Identity=23.6363636363636, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321016, Length=253, Percent_Identity=30.4347826086957, Blast_Score=97, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 54178; Mature: 54178

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRTHEAIPEIMYRIGDLADETGLPCYIVGGYVRDLIMQRPCTDIDIMVIGEPVPFAHA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LAEKLGGRNFVLFERFRTAQLELDDPKAGTFKLEIVGARKESYNPESRKPITSIGTLEDD
HHHHHCCCCEEEEEHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHH
LSRRDFTINALALRLNRKERGEVVDLFDGQRHIREKLLKTPLDPEQTFSDDPLRMMRAAR
HCCCCEEEEHHHEEECCCCCCCEEECCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHH
FACQLDFQLDEATLTAMSTMSSRIQIVSRERVSHEFFKIMEARKPSIGLKILYSTGLLKE
HHHEECCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
IIPELTVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PGSGWTFHGHEAVGVKIVARIFRAMKWPLEPMEYVQKMVRLHHRPIPLSKEEITDSAVRR
CCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMFDAGPDLDDLMTLCRADVTSKNPRKVQRIMKNFSNVEAKIAEVGEKDLLAKWRPPVSG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
TDIMELLNLPQGRTVGIIKSRMENAIIDGDIPYDRQAALDYIQQVYREMQEKGEA
HHHHHHHCCCCCCCHHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTRTHEAIPEIMYRIGDLADETGLPCYIVGGYVRDLIMQRPCTDIDIMVIGEPVPFAHA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHCCCCCEEEEEECCCCCHHHH
LAEKLGGRNFVLFERFRTAQLELDDPKAGTFKLEIVGARKESYNPESRKPITSIGTLEDD
HHHHHCCCCEEEEEHHCCCEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCHHHCCCHHH
LSRRDFTINALALRLNRKERGEVVDLFDGQRHIREKLLKTPLDPEQTFSDDPLRMMRAAR
HCCCCEEEEHHHEEECCCCCCCEEECCCCHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHH
FACQLDFQLDEATLTAMSTMSSRIQIVSRERVSHEFFKIMEARKPSIGLKILYSTGLLKE
HHHEECCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
IIPELTVMAGIEQVDGLGHKDTLFHTFQVVDNLAEHSDKLWLRVSALFHDIAKPVTKRFH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
PGSGWTFHGHEAVGVKIVARIFRAMKWPLEPMEYVQKMVRLHHRPIPLSKEEITDSAVRR
CCCCCEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
LMFDAGPDLDDLMTLCRADVTSKNPRKVQRIMKNFSNVEAKIAEVGEKDLLAKWRPPVSG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCC
TDIMELLNLPQGRTVGIIKSRMENAIIDGDIPYDRQAALDYIQQVYREMQEKGEA
HHHHHHHCCCCCCCHHHHHHHHHHCEECCCCCCCHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA