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The map label for this gene is amt-2 [C]

Identifier: 21673814

GI number: 21673814

Start: 933265

End: 934590

Strand: Reverse

Name: amt-2 [C]

Synonym: CT0986

Alternate gene names: 21673814

Gene position: 934590-933265 (Counterclockwise)

Preceding gene: 21673817

Following gene: 21673813

Centisome position: 43.37

GC content: 58.6

Gene sequence:

>1326_bases
ATGAAATCATTTCTGTCGAAATCCGGAGCGATTGCCGCTGGCCTCCTGCTGGCAGCGGCAACCTTCAGTCCGCCAATCCA
GGCGGCAACTCCGACGCCTGATGCCGTCAATGCATTCGCCATCGACAACTTCTTCCTGTTCATCTGCGCCGTGCTGGTGC
TGTTCATGCAGGCAGGCTTCGCGCTCGTTGAAACCGGACTGAACTCCGCCAAGAACGCCGTGAACATCTTGTTCAAGAAC
CTTATGGACATGGCGATCGGCGGTATCATTTACTATTTCGTCGGCTACGGCCTGATGTATCCTGGTGAAGCCTTCAGCGG
AGGATTCTTCGGATTTGGCGGCCCTGGCATCAGCACGGCCATGCCTGAAATCGCAGGAGGCACGCTCTATCCGGCAGTTG
ACTTCCTGTTCCAGGTTGCCTTCGCCGCCACAGCCGCCACGATCGTCTCGGGCGCCGTTGCAGGAAGGATGCAGTTCAGG
GCCTACCTGATCTATTCTGCGGTCATCTCGGCTGTTGTCTATCCCATCAGCGGCTTCTGGCTCTGGGGCGGCGGCTGGCT
GAAAGCGCTCGGCTTCCATGACTTCGCCGGTTCGCTTCTGGTGCACGCGCTCGGTGGTTTTGCCGGTCTCGCTGGTGCCA
TCGTGCTCGGCCCACGTATCGGAAGGTTCAATGAAGACGGCACCCCGAACGCTATGCCTGGCCACAACCTGGCGCTCAGC
ACCCTGGGCGTGTTCATTCTCCTGATCGGCTGGTACGGCTTCAACCCCGGCAGCCAGCTTGCCATCGTTGGTGGCGACAA
CACGGCCGCCGTCATGAAGATCGCGGTCAACACCACTCTGGCGGCCTGCGCTGGTGCCGTGGTCGCCATGATCTTCGCCT
GGGGCCTCTTCAAGAAACCTGACCTCACGATGGCGCTGAACGGTATGCTTGCCGGTCTGGTTGCCATCACGGCCAACTGC
GATGTGGTCTCCTATAATGCATCGCTGATCATCGGCGCTGTTGGCGGTATCCTTGTCGTGCTCGGTATCATGCTGCTCGA
CAAGCTCCGGATTGATGACCCGGTCGGCGCATGGCCGGTGCATGGACTAAACGGCATCTGGGGCGGTGTGGCTGCCTGGA
TCTTCGGCGGTCAGCCGATGGTTGCCCAGCTTGTCGGCTCACTGGTTGTGCCGTTCTGGGGATTCGTCACCATGATGGTG
CTGTTCCTGATCCTCAAGGCTATGGGTATCCTGCGCGTCCACAAGGATGAGGAGATGAAAGGCCTCGATATTTCAGAACA
TGAAGAAGAAGCTTATTATGGCTTCGATATCTACACAACCCAGTAA

Upstream 100 bases:

>100_bases
TATTAAAATTTTATATTTAAAACATGCGATGAGTTAAAAAACTCACGCCATACAACACAGCTGACTTTATTTTTTTTATC
AACCGTAACGGCTCCAGATC

Downstream 100 bases:

>100_bases
AGCATTTAAACGGAGCACAAGCAATGAAATACATCGTAGCAATGATACAGCCCCACAAGCTGCCGGACGTCAAGAAAAGC
CTTGCCGCAGCGGGGATACG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MKSFLSKSGAIAAGLLLAAATFSPPIQAATPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNSAKNAVNILFKN
LMDMAIGGIIYYFVGYGLMYPGEAFSGGFFGFGGPGISTAMPEIAGGTLYPAVDFLFQVAFAATAATIVSGAVAGRMQFR
AYLIYSAVISAVVYPISGFWLWGGGWLKALGFHDFAGSLLVHALGGFAGLAGAIVLGPRIGRFNEDGTPNAMPGHNLALS
TLGVFILLIGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAITANC
DVVSYNASLIIGAVGGILVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGVAAWIFGGQPMVAQLVGSLVVPFWGFVTMMV
LFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ

Sequences:

>Translated_441_residues
MKSFLSKSGAIAAGLLLAAATFSPPIQAATPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNSAKNAVNILFKN
LMDMAIGGIIYYFVGYGLMYPGEAFSGGFFGFGGPGISTAMPEIAGGTLYPAVDFLFQVAFAATAATIVSGAVAGRMQFR
AYLIYSAVISAVVYPISGFWLWGGGWLKALGFHDFAGSLLVHALGGFAGLAGAIVLGPRIGRFNEDGTPNAMPGHNLALS
TLGVFILLIGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAITANC
DVVSYNASLIIGAVGGILVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGVAAWIFGGQPMVAQLVGSLVVPFWGFVTMMV
LFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ
>Mature_441_residues
MKSFLSKSGAIAAGLLLAAATFSPPIQAATPTPDAVNAFAIDNFFLFICAVLVLFMQAGFALVETGLNSAKNAVNILFKN
LMDMAIGGIIYYFVGYGLMYPGEAFSGGFFGFGGPGISTAMPEIAGGTLYPAVDFLFQVAFAATAATIVSGAVAGRMQFR
AYLIYSAVISAVVYPISGFWLWGGGWLKALGFHDFAGSLLVHALGGFAGLAGAIVLGPRIGRFNEDGTPNAMPGHNLALS
TLGVFILLIGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKPDLTMALNGMLAGLVAITANC
DVVSYNASLIIGAVGGILVVLGIMLLDKLRIDDPVGAWPVHGLNGIWGGVAAWIFGGQPMVAQLVGSLVVPFWGFVTMMV
LFLILKAMGILRVHKDEEMKGLDISEHEEEAYYGFDIYTTQ

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=415, Percent_Identity=33.9759036144578, Blast_Score=174, Evalue=7e-45,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=33.5648148148148, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI32564042, Length=428, Percent_Identity=32.4766355140187, Blast_Score=197, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17550182, Length=433, Percent_Identity=36.0277136258661, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI133931100, Length=422, Percent_Identity=31.042654028436, Blast_Score=176, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17563228, Length=255, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=27.7777777777778, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=445, Percent_Identity=27.8651685393258, Blast_Score=126, Evalue=6e-30,
Organism=Saccharomyces cerevisiae, GI6321559, Length=422, Percent_Identity=27.2511848341232, Blast_Score=117, Evalue=4e-27,
Organism=Drosophila melanogaster, GI161078314, Length=409, Percent_Identity=34.4743276283619, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047
- InterPro:   IPR002229 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46147; Mature: 46147

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFLSKSGAIAAGLLLAAATFSPPIQAATPTPDAVNAFAIDNFFLFICAVLVLFMQAGF
CCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
ALVETGLNSAKNAVNILFKNLMDMAIGGIIYYFVGYGLMYPGEAFSGGFFGFGGPGISTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
MPEIAGGTLYPAVDFLFQVAFAATAATIVSGAVAGRMQFRAYLIYSAVISAVVYPISGFW
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LWGGGWLKALGFHDFAGSLLVHALGGFAGLAGAIVLGPRIGRFNEDGTPNAMPGHNLALS
EECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
TLGVFILLIGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKP
HHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTMALNGMLAGLVAITANCDVVSYNASLIIGAVGGILVVLGIMLLDKLRIDDPVGAWPV
CHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HGLNGIWGGVAAWIFGGQPMVAQLVGSLVVPFWGFVTMMVLFLILKAMGILRVHKDEEMK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
GLDISEHEEEAYYGFDIYTTQ
CCCCCCCCCCCEEEEEEEECC
>Mature Secondary Structure
MKSFLSKSGAIAAGLLLAAATFSPPIQAATPTPDAVNAFAIDNFFLFICAVLVLFMQAGF
CCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
ALVETGLNSAKNAVNILFKNLMDMAIGGIIYYFVGYGLMYPGEAFSGGFFGFGGPGISTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHC
MPEIAGGTLYPAVDFLFQVAFAATAATIVSGAVAGRMQFRAYLIYSAVISAVVYPISGFW
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LWGGGWLKALGFHDFAGSLLVHALGGFAGLAGAIVLGPRIGRFNEDGTPNAMPGHNLALS
EECCHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
TLGVFILLIGWYGFNPGSQLAIVGGDNTAAVMKIAVNTTLAACAGAVVAMIFAWGLFKKP
HHHHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCC
DLTMALNGMLAGLVAITANCDVVSYNASLIIGAVGGILVVLGIMLLDKLRIDDPVGAWPV
CHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
HGLNGIWGGVAAWIFGGQPMVAQLVGSLVVPFWGFVTMMVLFLILKAMGILRVHKDEEMK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
GLDISEHEEEAYYGFDIYTTQ
CCCCCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]