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The map label for this gene is mltD [H]

Identifier: 21673807

GI number: 21673807

Start: 926280

End: 927890

Strand: Direct

Name: mltD [H]

Synonym: CT0979

Alternate gene names: 21673807

Gene position: 926280-927890 (Clockwise)

Preceding gene: 21673806

Following gene: 21673808

Centisome position: 42.98

GC content: 53.32

Gene sequence:

>1611_bases
ATGGGCGCTCATCTGTTCTTTTGTCTCTTTGCGGCGGCTCCTTGTATTAATGGTGCTCTCTGGTTATGTTTCCAAATTAT
ACTTTACTGTCTCAAACCCGAGGGTGCCAGCGCAACCTCATCTGTTAACCCAGCCCGGCTTGTGAAAAGCTTTTTACCGC
TCAGGCATTTTCTTGTCTGTATATCCTTGCAGGTTGTTGCTCTGTCTTCCGTATCGTTTGGTGCGGAGGAACCCGACAGC
GCCTCGAACGTGCGAGCCTCACGTGTTTCGGAAATGCTCGATAGCCTCGTTACGGCTACCTATTTTCAGGATGACCGGTT
TTCTTCGGCGGATAGGAGCGGCTCCGCCGGGTATCTTCCCAAAGAGTTCATCCCCCAGTTTAGCGACTCTGTTTATGCGT
CAAGAATTGCTGCGCTCGCCAGCAAAAGCCGCTTCAATCTGGTTTATAATGATCATGTAAAAGGCTTTATTCGGCTTTAT
GCCGTTGACAAGAGGAAAATGGTATCGAAGGTGCTTGGCCTGACGCATATCTATTTTCCGCTCTTTGACGAAGTGTTCAG
GCAGTACGATATCCCTCCTGAAATGAAGTACCTCGCCATCGTGGAGTCTGCACTGAACCCCACGGCAGTGTCCCGGGCCG
GCGCCCGTGGTCTCTGGCAGTTCATGAGTGGTACGGGCCGGATGTACGGATTGCAATCCTCTTCGTTCATTGAGGATCGC
TATGACCCCACAAAAGCGACCGTGGCGGCGTGCCAGCATCTTCGTGATCTGTATGACATGTTTGGCGACTGGTTTCTCGT
TCTCGCGGCCTACAATGCCGGAGCCGGCAATGTGCAGCGGGCGATCAGAAGAGCTGGCGGCGCTCACGATTACTGGGAGA
TATGGCCATACCTGCCGCAGGAGACGCGGGGTTACGTTCCGGCCTTCATCGCGGTGACGTACGTCATGAACTACTACCGC
GAGCACAACATCCGTCCCGCCCAGCCGGGATACCTCTACTCAGAGACCGCCATGGTTCCGGTCAAGCAGGCGCTGACCTT
CGAGCAGCTCAACGAGACGCTTGGCGTTTCAATGGACGACCTCAAATTCCTCAATCCCCAGTACAAAGCGGGCCTCATTC
CTGCCCCTGCATCCAGCCCCAACATGATCCGGCTTCCGAAACAATACGTGCAGCTGTTCCTGCAAAAGGAGCAGCAGATC
TATGCGTACAAGCCGGAGATGGTTGAAGAGAAGGAGCGTCTGTATGCCATGGTTCAGGAGAACGACCGGCAGGATGTGGT
GTTTAGCAGTGGCCGGGGCCAAAAGACGCACGTGGTTGGCAAACGGGAGACACTCGCAAGCGTTGCTCGTAAATATGGCT
GTTCGGTCAGTCAGATTATTGACTGGAACAATCTGAAAAGTGCAAAGCTGAGGCCGGGCCAGCGGCTGGTGGTATTCAAG
GTGGTCAATGAGCGATTCTCCCGAAACGCATCTGCCACGAAACTCAAGGGCAAAAAAGGCCGACTGAAATCAAAGGCGAG
CAACGCTGCAGGCAAAAGCAAAGTTCTCAAGAAAAGCAAGAGCGGTAAAAAGGCCGGTAAGAAGAGCGGGACAAAGAAAA
AACGCAAATAA

Upstream 100 bases:

>100_bases
AAATAAATTATGAAATTATGAGTGATGAATGATGCATTGGTATGGGAAGGGGCTGCCGAGAGATTTTCGAGCGGGGTGAT
TCCGGAACTCCTTTTAATGG

Downstream 100 bases:

>100_bases
CAGATCACAGATTACAGATGCGCTTCACGGGATTGCAGACTGTTGGCGATTCTGACCAGAATCAGCGATGGTTTGACCAG
TTGTTCTGCTTGTCATTGTA

Product: membrane-bound lytic murein transglycosylase, putative

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MGAHLFFCLFAAAPCINGALWLCFQIILYCLKPEGASATSSVNPARLVKSFLPLRHFLVCISLQVVALSSVSFGAEEPDS
ASNVRASRVSEMLDSLVTATYFQDDRFSSADRSGSAGYLPKEFIPQFSDSVYASRIAALASKSRFNLVYNDHVKGFIRLY
AVDKRKMVSKVLGLTHIYFPLFDEVFRQYDIPPEMKYLAIVESALNPTAVSRAGARGLWQFMSGTGRMYGLQSSSFIEDR
YDPTKATVAACQHLRDLYDMFGDWFLVLAAYNAGAGNVQRAIRRAGGAHDYWEIWPYLPQETRGYVPAFIAVTYVMNYYR
EHNIRPAQPGYLYSETAMVPVKQALTFEQLNETLGVSMDDLKFLNPQYKAGLIPAPASSPNMIRLPKQYVQLFLQKEQQI
YAYKPEMVEEKERLYAMVQENDRQDVVFSSGRGQKTHVVGKRETLASVARKYGCSVSQIIDWNNLKSAKLRPGQRLVVFK
VVNERFSRNASATKLKGKKGRLKSKASNAAGKSKVLKKSKSGKKAGKKSGTKKKRK

Sequences:

>Translated_536_residues
MGAHLFFCLFAAAPCINGALWLCFQIILYCLKPEGASATSSVNPARLVKSFLPLRHFLVCISLQVVALSSVSFGAEEPDS
ASNVRASRVSEMLDSLVTATYFQDDRFSSADRSGSAGYLPKEFIPQFSDSVYASRIAALASKSRFNLVYNDHVKGFIRLY
AVDKRKMVSKVLGLTHIYFPLFDEVFRQYDIPPEMKYLAIVESALNPTAVSRAGARGLWQFMSGTGRMYGLQSSSFIEDR
YDPTKATVAACQHLRDLYDMFGDWFLVLAAYNAGAGNVQRAIRRAGGAHDYWEIWPYLPQETRGYVPAFIAVTYVMNYYR
EHNIRPAQPGYLYSETAMVPVKQALTFEQLNETLGVSMDDLKFLNPQYKAGLIPAPASSPNMIRLPKQYVQLFLQKEQQI
YAYKPEMVEEKERLYAMVQENDRQDVVFSSGRGQKTHVVGKRETLASVARKYGCSVSQIIDWNNLKSAKLRPGQRLVVFK
VVNERFSRNASATKLKGKKGRLKSKASNAAGKSKVLKKSKSGKKAGKKSGTKKKRK
>Mature_535_residues
GAHLFFCLFAAAPCINGALWLCFQIILYCLKPEGASATSSVNPARLVKSFLPLRHFLVCISLQVVALSSVSFGAEEPDSA
SNVRASRVSEMLDSLVTATYFQDDRFSSADRSGSAGYLPKEFIPQFSDSVYASRIAALASKSRFNLVYNDHVKGFIRLYA
VDKRKMVSKVLGLTHIYFPLFDEVFRQYDIPPEMKYLAIVESALNPTAVSRAGARGLWQFMSGTGRMYGLQSSSFIEDRY
DPTKATVAACQHLRDLYDMFGDWFLVLAAYNAGAGNVQRAIRRAGGAHDYWEIWPYLPQETRGYVPAFIAVTYVMNYYRE
HNIRPAQPGYLYSETAMVPVKQALTFEQLNETLGVSMDDLKFLNPQYKAGLIPAPASSPNMIRLPKQYVQLFLQKEQQIY
AYKPEMVEEKERLYAMVQENDRQDVVFSSGRGQKTHVVGKRETLASVARKYGCSVSQIIDWNNLKSAKLRPGQRLVVFKV
VNERFSRNASATKLKGKKGRLKSKASNAAGKSKVLKKSKSGKKAGKKSGTKKKRK

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=323, Percent_Identity=29.7213622291022, Blast_Score=143, Evalue=3e-35,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 60083; Mature: 59952

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGAHLFFCLFAAAPCINGALWLCFQIILYCLKPEGASATSSVNPARLVKSFLPLRHFLVC
CCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ISLQVVALSSVSFGAEEPDSASNVRASRVSEMLDSLVTATYFQDDRFSSADRSGSAGYLP
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KEFIPQFSDSVYASRIAALASKSRFNLVYNDHVKGFIRLYAVDKRKMVSKVLGLTHIYFP
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHEEHHHEEHHHHHHHHHHHHHHHHHHH
LFDEVFRQYDIPPEMKYLAIVESALNPTAVSRAGARGLWQFMSGTGRMYGLQSSSFIEDR
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHC
YDPTKATVAACQHLRDLYDMFGDWFLVLAAYNAGAGNVQRAIRRAGGAHDYWEIWPYLPQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCCC
ETRGYVPAFIAVTYVMNYYREHNIRPAQPGYLYSETAMVPVKQALTFEQLNETLGVSMDD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHH
LKFLNPQYKAGLIPAPASSPNMIRLPKQYVQLFLQKEQQIYAYKPEMVEEKERLYAMVQE
HHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHC
NDRQDVVFSSGRGQKTHVVGKRETLASVARKYGCSVSQIIDWNNLKSAKLRPGQRLVVFK
CCCCCEEEECCCCCCEEECCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEHH
VVNERFSRNASATKLKGKKGRLKSKASNAAGKSKVLKKSKSGKKAGKKSGTKKKRK
HHHHHHHCCCCHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
GAHLFFCLFAAAPCINGALWLCFQIILYCLKPEGASATSSVNPARLVKSFLPLRHFLVC
CHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ISLQVVALSSVSFGAEEPDSASNVRASRVSEMLDSLVTATYFQDDRFSSADRSGSAGYLP
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
KEFIPQFSDSVYASRIAALASKSRFNLVYNDHVKGFIRLYAVDKRKMVSKVLGLTHIYFP
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHEEHHHEEHHHHHHHHHHHHHHHHHHH
LFDEVFRQYDIPPEMKYLAIVESALNPTAVSRAGARGLWQFMSGTGRMYGLQSSSFIEDR
HHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHC
YDPTKATVAACQHLRDLYDMFGDWFLVLAAYNAGAGNVQRAIRRAGGAHDYWEIWPYLPQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHCCCCCC
ETRGYVPAFIAVTYVMNYYREHNIRPAQPGYLYSETAMVPVKQALTFEQLNETLGVSMDD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHCCCHHH
LKFLNPQYKAGLIPAPASSPNMIRLPKQYVQLFLQKEQQIYAYKPEMVEEKERLYAMVQE
HHHCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHC
NDRQDVVFSSGRGQKTHVVGKRETLASVARKYGCSVSQIIDWNNLKSAKLRPGQRLVVFK
CCCCCEEEECCCCCCEEECCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCEEEEHH
VVNERFSRNASATKLKGKKGRLKSKASNAAGKSKVLKKSKSGKKAGKKSGTKKKRK
HHHHHHHCCCCHHHCCCCCCCHHHHHHCCCHHHHHHHHHCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]