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The map label for this gene is metG

Identifier: 21673797

GI number: 21673797

Start: 913219

End: 915330

Strand: Direct

Name: metG

Synonym: CT0969

Alternate gene names: 21673797

Gene position: 913219-915330 (Clockwise)

Preceding gene: 21673796

Following gene: 21673798

Centisome position: 42.38

GC content: 59.14

Gene sequence:

>2112_bases
ATGACTCATATACCCAAAAGAACTCTCGTTACCACCGCTCTTCCATATGCCAACGGCCCGGTGCACCTCGGCCACCTCGC
CGGGGTCTATCTGCCCGCCGATATTTATGTTCGCTACAAGCGCCTCTGCGGCCACGACGTGATCCATATCGGTGGCTCGG
ATGAGCACGGCGTGCCGATTACCATTACCGCCGACAAGGAGGGCATTTCGCCGCAGGAAGTGGTGGATCGCTACCACACA
ATGAATGCTGAGGCATTTGCGAAGTGCGGCATTTCGTTTGATTACTACGGGCGCACCAGTGGGCCGGTTCATCACCAGAC
CGCTCGTGAGTTTTTCTTGGAAATCGAGAAAAAGGGGATTTTTGTCAAGAAAACCGAAAAGCAGTTCTTCGATCCGAAGG
CGGGGCGCTTCCTCTCCGACCGCTACATCACCGGCACCTGCCCGGTCTGCAAAACGCCCGGCGCGAACGGCGACCAGTGC
GAGCAGTGCGGCACGCACCTCAGCCCGACCGAGCTGATCGATCCGAAGAGCAAGCTCTCCGACGCTACGCCGGAGCTGCG
CGAAACCCTGCACTGGTACTTCCCGCTTGGGCGCTACCAGAAGCAGCTCGAAGCCTTTGTTGAACGGCACACCGGTGACT
GGCGCTCTAACGTTGTCAACTACTCACGCACGTGGCTCAACCAGGGCCTCGCCGACCGTGCGATCACCCGCGATCTCGCT
TGGGGCATCTCGCTGCCGCTCGACTCCGAAGAGGCGAAAGGCAAGGTGCTTTATGTCTGGTTCGACGCCGTGCTTGGCTA
CATTTCATTTACGAAAGAGTGGGCTGAAAAGCAGGGCGACGCCGAACTCTGGCGGCGCTACTGGCAGGATCCGGAGACGC
GCATCATCAACTTCATCGGCAAGGATAACGTCGTGTTCCACACGCTGATGTTCCCGGCGATTCTGATGGCCTGGAACGAG
GGGCGCAGCGAGGGGCGCTACGAGCTGGCCGACAATGTTCCGGCCTCCGAGTTCATGAACTTCGAGGGGCGCAAGTTCTC
AAAGTCGCGCAACTATGCAGTTTATCTCGGCGAGTTTCTCGAACGCTTCCCGGCGGACACGTTGCGCTACAGCATCGCGA
TGAACTACCCGGAGAACAAGGATACCGACTTCAGCTGGAGTGACTTCCAGAACCGCACCAACGGCGAGCTGGCCGACACG
CTCGGCAACTTCATCAAGCGTTCGATCGACTTTACCAACTCGCGCTTCGGCGGCCAGGTGCCAGCGGACATAGACCTTGA
AGCGTGGGACAGCCTCGGCATCGACTGGCTGGCGAGTTTCGGCAAACTCGAAGCGGCCTACGACGGCTTCCACTTCCGTG
AAGCAACCGCACAGACGATGGAGATCGCGCGTTTCGCCAATCGCTTTCTCACCGAGAGCGAGCCGTGGAAAGTCATTAAG
GTCGATCCGGAAGCGGCGGGGCGCACGATGGCCGTGTCGCTGAATCTTTGCCACACGCTCGCGCTGCTCTTCTGGCCGAT
TGTGCCCGAAACCGCCAACCGCATTTGGAAGATGCTCGGTTTCGAGGGCACGATTGACGAACTCGTTGAACCCGGCAACC
CTGTCTGGCGTCAGGCGCTCGAACCGGGGCTGAAGAAGGGACACAAGCTGCTTGGTTCGTCCGAAATTCTTTTCTCGAAG
ATCGAGGACAAGGACATCGAGCCAGAGATGAAGAAGATCGAGGCGCTGCTGGCCGAGGCGGAGCAGCGTGAAGCCGCCAA
GCAGCCCGTGCCGATGACCTTTAAGCCTGAGATCACCTTCGACGACTTCCAGAAGATCGATCTGCGTGTGGCAAAAGTGG
TCGCCTGCGAGCCGGTCAAAAAGGCCAACAAACTGCTCAAGCTGCAATTGCAGGTCGGCTCGGAGCAGCGCCAGGTGCTT
TCGGGCATCGCGCAGTACTTTACGCCCGAGCAGATGGTGGGCAAGAATGTCGTGCTGGTGGCCAATCTCGCTGACCGCAC
CATGCGGGGCGAGCTGTCGCAGGGCATGATCCTCACTGTCGAGGGCGCCGATGGCCGCCTTTTTCTGCTCGAACCGCAGG
GCGAAGGAATAAATGGGAACTCTGTCAGTTAA

Upstream 100 bases:

>100_bases
CCGGAAAGCGTCAGGGCGGACAGGCCTGAAACACCATTTCATCACCCGGCAATCCGCCGGGTGACGTGATTTTATCATCA
TCGAACGACTTGTGTTACCA

Downstream 100 bases:

>100_bases
CCATTTGTTTTTTACAATGAAAGTCGTACATTAAGCAGCTTTTACATCGTGGGGTGGAGCAATTGGTAGCTCGTCGGGCT
CATAACCCGAAGGTTGCAGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 703; Mature: 702

Protein sequence:

>703_residues
MTHIPKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYKRLCGHDVIHIGGSDEHGVPITITADKEGISPQEVVDRYHT
MNAEAFAKCGISFDYYGRTSGPVHHQTAREFFLEIEKKGIFVKKTEKQFFDPKAGRFLSDRYITGTCPVCKTPGANGDQC
EQCGTHLSPTELIDPKSKLSDATPELRETLHWYFPLGRYQKQLEAFVERHTGDWRSNVVNYSRTWLNQGLADRAITRDLA
WGISLPLDSEEAKGKVLYVWFDAVLGYISFTKEWAEKQGDAELWRRYWQDPETRIINFIGKDNVVFHTLMFPAILMAWNE
GRSEGRYELADNVPASEFMNFEGRKFSKSRNYAVYLGEFLERFPADTLRYSIAMNYPENKDTDFSWSDFQNRTNGELADT
LGNFIKRSIDFTNSRFGGQVPADIDLEAWDSLGIDWLASFGKLEAAYDGFHFREATAQTMEIARFANRFLTESEPWKVIK
VDPEAAGRTMAVSLNLCHTLALLFWPIVPETANRIWKMLGFEGTIDELVEPGNPVWRQALEPGLKKGHKLLGSSEILFSK
IEDKDIEPEMKKIEALLAEAEQREAAKQPVPMTFKPEITFDDFQKIDLRVAKVVACEPVKKANKLLKLQLQVGSEQRQVL
SGIAQYFTPEQMVGKNVVLVANLADRTMRGELSQGMILTVEGADGRLFLLEPQGEGINGNSVS

Sequences:

>Translated_703_residues
MTHIPKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYKRLCGHDVIHIGGSDEHGVPITITADKEGISPQEVVDRYHT
MNAEAFAKCGISFDYYGRTSGPVHHQTAREFFLEIEKKGIFVKKTEKQFFDPKAGRFLSDRYITGTCPVCKTPGANGDQC
EQCGTHLSPTELIDPKSKLSDATPELRETLHWYFPLGRYQKQLEAFVERHTGDWRSNVVNYSRTWLNQGLADRAITRDLA
WGISLPLDSEEAKGKVLYVWFDAVLGYISFTKEWAEKQGDAELWRRYWQDPETRIINFIGKDNVVFHTLMFPAILMAWNE
GRSEGRYELADNVPASEFMNFEGRKFSKSRNYAVYLGEFLERFPADTLRYSIAMNYPENKDTDFSWSDFQNRTNGELADT
LGNFIKRSIDFTNSRFGGQVPADIDLEAWDSLGIDWLASFGKLEAAYDGFHFREATAQTMEIARFANRFLTESEPWKVIK
VDPEAAGRTMAVSLNLCHTLALLFWPIVPETANRIWKMLGFEGTIDELVEPGNPVWRQALEPGLKKGHKLLGSSEILFSK
IEDKDIEPEMKKIEALLAEAEQREAAKQPVPMTFKPEITFDDFQKIDLRVAKVVACEPVKKANKLLKLQLQVGSEQRQVL
SGIAQYFTPEQMVGKNVVLVANLADRTMRGELSQGMILTVEGADGRLFLLEPQGEGINGNSVS
>Mature_702_residues
THIPKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYKRLCGHDVIHIGGSDEHGVPITITADKEGISPQEVVDRYHTM
NAEAFAKCGISFDYYGRTSGPVHHQTAREFFLEIEKKGIFVKKTEKQFFDPKAGRFLSDRYITGTCPVCKTPGANGDQCE
QCGTHLSPTELIDPKSKLSDATPELRETLHWYFPLGRYQKQLEAFVERHTGDWRSNVVNYSRTWLNQGLADRAITRDLAW
GISLPLDSEEAKGKVLYVWFDAVLGYISFTKEWAEKQGDAELWRRYWQDPETRIINFIGKDNVVFHTLMFPAILMAWNEG
RSEGRYELADNVPASEFMNFEGRKFSKSRNYAVYLGEFLERFPADTLRYSIAMNYPENKDTDFSWSDFQNRTNGELADTL
GNFIKRSIDFTNSRFGGQVPADIDLEAWDSLGIDWLASFGKLEAAYDGFHFREATAQTMEIARFANRFLTESEPWKVIKV
DPEAAGRTMAVSLNLCHTLALLFWPIVPETANRIWKMLGFEGTIDELVEPGNPVWRQALEPGLKKGHKLLGSSEILFSKI
EDKDIEPEMKKIEALLAEAEQREAAKQPVPMTFKPEITFDDFQKIDLRVAKVVACEPVKKANKLLKLQLQVGSEQRQVLS
GIAQYFTPEQMVGKNVVLVANLADRTMRGELSQGMILTVEGADGRLFLLEPQGEGINGNSVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=644, Percent_Identity=32.2981366459627, Blast_Score=355, Evalue=1e-97,
Organism=Homo sapiens, GI24308436, Length=552, Percent_Identity=25.9057971014493, Blast_Score=136, Evalue=5e-32,
Organism=Escherichia coli, GI1788432, Length=714, Percent_Identity=34.3137254901961, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1789455, Length=97, Percent_Identity=35.0515463917526, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17540794, Length=572, Percent_Identity=34.6153846153846, Blast_Score=377, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI212645096, Length=534, Percent_Identity=23.9700374531835, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71992781, Length=361, Percent_Identity=24.9307479224377, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321704, Length=570, Percent_Identity=33.3333333333333, Blast_Score=346, Evalue=6e-96,
Organism=Saccharomyces cerevisiae, GI6321610, Length=551, Percent_Identity=21.9600725952813, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24655311, Length=575, Percent_Identity=33.5652173913043, Blast_Score=342, Evalue=6e-94,
Organism=Drosophila melanogaster, GI21358483, Length=588, Percent_Identity=23.8095238095238, Blast_Score=119, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355409, Length=222, Percent_Identity=26.1261261261261, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHLTE (P59077)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_661862.1
- ProteinModelPortal:   P59077
- SMR:   P59077
- GeneID:   1007067
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0969
- NMPDR:   fig|194439.1.peg.956
- TIGR:   CT0969
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   CTEP194439:CT_0969-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 79554; Mature: 79423

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 348-348

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHIPKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYKRLCGHDVIHIGGSDEHGVPI
CCCCCCCHHHHEECCCCCCCEEEHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCCCEEE
TITADKEGISPQEVVDRYHTMNAEAFAKCGISFDYYGRTSGPVHHQTAREFFLEIEKKGI
EEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCE
FVKKTEKQFFDPKAGRFLSDRYITGTCPVCKTPGANGDQCEQCGTHLSPTELIDPKSKLS
EEEECHHHHCCCCCCCEECCCEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCHHHHC
DATPELRETLHWYFPLGRYQKQLEAFVERHTGDWRSNVVNYSRTWLNQGLADRAITRDLA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
WGISLPLDSEEAKGKVLYVWFDAVLGYISFTKEWAEKQGDAELWRRYWQDPETRIINFIG
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCC
KDNVVFHTLMFPAILMAWNEGRSEGRYELADNVPASEFMNFEGRKFSKSRNYAVYLGEFL
CCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHCCCCCCCCCCCCCEEEEHHHHH
ERFPADTLRYSIAMNYPENKDTDFSWSDFQNRTNGELADTLGNFIKRSIDFTNSRFGGQV
HHCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PADIDLEAWDSLGIDWLASFGKLEAAYDGFHFREATAQTMEIARFANRFLTESEPWKVIK
CCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDPEAAGRTMAVSLNLCHTLALLFWPIVPETANRIWKMLGFEGTIDELVEPGNPVWRQAL
ECCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
EPGLKKGHKLLGSSEILFSKIEDKDIEPEMKKIEALLAEAEQREAAKQPVPMTFKPEITF
HHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCH
DDFQKIDLRVAKVVACEPVKKANKLLKLQLQVGSEQRQVLSGIAQYFTPEQMVGKNVVLV
HHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCHHHHCCCEEEE
ANLADRTMRGELSQGMILTVEGADGRLFLLEPQGEGINGNSVS
EECHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
THIPKRTLVTTALPYANGPVHLGHLAGVYLPADIYVRYKRLCGHDVIHIGGSDEHGVPI
CCCCCCHHHHEECCCCCCCEEEHHHCCEEECHHHHHHHHHHCCCCEEEECCCCCCCEEE
TITADKEGISPQEVVDRYHTMNAEAFAKCGISFDYYGRTSGPVHHQTAREFFLEIEKKGI
EEECCCCCCCHHHHHHHHHHCCHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCE
FVKKTEKQFFDPKAGRFLSDRYITGTCPVCKTPGANGDQCEQCGTHLSPTELIDPKSKLS
EEEECHHHHCCCCCCCEECCCEEECCCCCCCCCCCCCHHHHHCCCCCCCHHHCCCHHHHC
DATPELRETLHWYFPLGRYQKQLEAFVERHTGDWRSNVVNYSRTWLNQGLADRAITRDLA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHH
WGISLPLDSEEAKGKVLYVWFDAVLGYISFTKEWAEKQGDAELWRRYWQDPETRIINFIG
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHCC
KDNVVFHTLMFPAILMAWNEGRSEGRYELADNVPASEFMNFEGRKFSKSRNYAVYLGEFL
CCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHCCCCCCCCCCCCCEEEEHHHHH
ERFPADTLRYSIAMNYPENKDTDFSWSDFQNRTNGELADTLGNFIKRSIDFTNSRFGGQV
HHCCCCCEEEEEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
PADIDLEAWDSLGIDWLASFGKLEAAYDGFHFREATAQTMEIARFANRFLTESEPWKVIK
CCCCCHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDPEAAGRTMAVSLNLCHTLALLFWPIVPETANRIWKMLGFEGTIDELVEPGNPVWRQAL
ECCCCCCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHH
EPGLKKGHKLLGSSEILFSKIEDKDIEPEMKKIEALLAEAEQREAAKQPVPMTFKPEITF
HHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCH
DDFQKIDLRVAKVVACEPVKKANKLLKLQLQVGSEQRQVLSGIAQYFTPEQMVGKNVVLV
HHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCHHHHCCCEEEE
ANLADRTMRGELSQGMILTVEGADGRLFLLEPQGEGINGNSVS
EECHHHHHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901