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The map label for this gene is cobS [H]

Identifier: 21673776

GI number: 21673776

Start: 895822

End: 896625

Strand: Direct

Name: cobS [H]

Synonym: CT0948

Alternate gene names: 21673776

Gene position: 895822-896625 (Clockwise)

Preceding gene: 21673775

Following gene: 21673777

Centisome position: 41.57

GC content: 64.43

Gene sequence:

>804_bases
TTGCCGGGGTTAAGAAATTTGTCGAAAAGCTCGTCTGAGATGCTGAGCGGACTCGTGACAGCGCTCCGGACGCTGACAGC
GCTGCCGGTGCCGGGGCGTGACGCCGAGCGGTTCAGCTCGTCGCTCTACTGGTTTCCGGTGGTTGGGCTCGTTATCGGCG
GCATCGTTGTGCTGCTTGCCCGCGCCGGAATGGGCGTCGGATGGCCGGAGCTGGCCGCCGTGCTCGCGCTGCTGGGTGGG
CTCATCCTCACCCGCGGGCTTCATGCCGACGGGCTGGCTGATCTCGCCGATGGCTTTTTCGGAGGGCGAACCCGCGAGGC
GGCGTTGCGGATTATGAAAGATCCAAACGTCGGCTCGTTCGGATCGCTGGCGCTCATCGGTGTCATGCTCTTCAAGTGGA
TTTGTCTGCTTGAACTGGCCCGCGCCGAGGCGTACGGCATGATCGCCGCCGGAGCCGTCCTGTCGCGCACGGCGCAGGTA
CTGCTTGCCGCGAGGATGCCTTACGCCCGCAGTGATGGCGGTACGGCTATGGCGTTTGTCGAGGATGCGGGCTGGCCGCA
CCTGCTCGTCGCCTCGATCTCCGGCGTCGTGTTGCTTTTCGTGCTGCTCGACTGGCAGCTCGCTCCATCGTTGATCCTGC
TCTTTGGCTCGGTTGTCGCCCTGTTCTTCGTCGGCTGGTTGAGTCACCGCAAGATCGGCGGCATCACGGGCGACGTGCTC
GGTGCCTGCAGCGAGCTGGTGGAGGCTGCCGTGTGGCTTTTGGCGGCGCTGTGGCTCAAAGGGCTTTTTTGGGCGATAGC
GTAG

Upstream 100 bases:

>100_bases
GAAGAGAGTTTCCGGCTGGCGCGTGAGCTGCCGAGGGCGGAGCTGCTCGTGATTCCCGATTGCGCTCATCTGCCGCAGGA
GGAGCAGCCCGCCGCGTTCG

Downstream 100 bases:

>100_bases
TGCCCGTTTGCGTTGTGCCCGTTTGCGTTCGGTGATTTTTTCAGCACGTTGCTAATCAATTGCTCTATCGTTATACTTGA
GCACGGAATTTTAATTTCAA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MPGLRNLSKSSSEMLSGLVTALRTLTALPVPGRDAERFSSSLYWFPVVGLVIGGIVVLLARAGMGVGWPELAAVLALLGG
LILTRGLHADGLADLADGFFGGRTREAALRIMKDPNVGSFGSLALIGVMLFKWICLLELARAEAYGMIAAGAVLSRTAQV
LLAARMPYARSDGGTAMAFVEDAGWPHLLVASISGVVLLFVLLDWQLAPSLILLFGSVVALFFVGWLSHRKIGGITGDVL
GACSELVEAAVWLLAALWLKGLFWAIA

Sequences:

>Translated_267_residues
MPGLRNLSKSSSEMLSGLVTALRTLTALPVPGRDAERFSSSLYWFPVVGLVIGGIVVLLARAGMGVGWPELAAVLALLGG
LILTRGLHADGLADLADGFFGGRTREAALRIMKDPNVGSFGSLALIGVMLFKWICLLELARAEAYGMIAAGAVLSRTAQV
LLAARMPYARSDGGTAMAFVEDAGWPHLLVASISGVVLLFVLLDWQLAPSLILLFGSVVALFFVGWLSHRKIGGITGDVL
GACSELVEAAVWLLAALWLKGLFWAIA
>Mature_266_residues
PGLRNLSKSSSEMLSGLVTALRTLTALPVPGRDAERFSSSLYWFPVVGLVIGGIVVLLARAGMGVGWPELAAVLALLGGL
ILTRGLHADGLADLADGFFGGRTREAALRIMKDPNVGSFGSLALIGVMLFKWICLLELARAEAYGMIAAGAVLSRTAQVL
LAARMPYARSDGGTAMAFVEDAGWPHLLVASISGVVLLFVLLDWQLAPSLILLFGSVVALFFVGWLSHRKIGGITGDVLG
ACSELVEAAVWLLAALWLKGLFWAIA

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=246, Percent_Identity=36.5853658536585, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28115; Mature: 27984

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGLRNLSKSSSEMLSGLVTALRTLTALPVPGRDAERFSSSLYWFPVVGLVIGGIVVLLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
RAGMGVGWPELAAVLALLGGLILTRGLHADGLADLADGFFGGRTREAALRIMKDPNVGSF
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCH
GSLALIGVMLFKWICLLELARAEAYGMIAAGAVLSRTAQVLLAARMPYARSDGGTAMAFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EDAGWPHLLVASISGVVLLFVLLDWQLAPSLILLFGSVVALFFVGWLSHRKIGGITGDVL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GACSELVEAAVWLLAALWLKGLFWAIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PGLRNLSKSSSEMLSGLVTALRTLTALPVPGRDAERFSSSLYWFPVVGLVIGGIVVLLA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHH
RAGMGVGWPELAAVLALLGGLILTRGLHADGLADLADGFFGGRTREAALRIMKDPNVGSF
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCH
GSLALIGVMLFKWICLLELARAEAYGMIAAGAVLSRTAQVLLAARMPYARSDGGTAMAFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EDAGWPHLLVASISGVVLLFVLLDWQLAPSLILLFGSVVALFFVGWLSHRKIGGITGDVL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
GACSELVEAAVWLLAALWLKGLFWAIA
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA