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The map label for this gene is birA [H]

Identifier: 21672894

GI number: 21672894

Start: 51107

End: 52108

Strand: Reverse

Name: birA [H]

Synonym: CT0053

Alternate gene names: 21672894

Gene position: 52108-51107 (Counterclockwise)

Preceding gene: 21672895

Following gene: 21672893

Centisome position: 2.42

GC content: 62.97

Gene sequence:

>1002_bases
ATGAATCCGGTGACGGCAACCATTCTGCAACGTCTCGCCGGTGATGGCGGATTTGTTTCCGGAGGCGATCTCTGTAACGA
GTTGCAGATCAGCCGGAGCGCGGTGTGGAAACATATTGTCGCTCTGAGAAAAGCCGGATATGCCATTGAGGCGACAAGCG
GCAAAGGGTACCGCCTTGAGAGTCTGACCGGCTCACCGGTTGCCGAAGAGGTCGCGCCGCTGCTGGCAACCGAGTCGTTC
GGGCGAAACTTCATCTATCTTGAAAGCATCGATTCGACCAACCTCCGCGCGCGGGCGTCGGCGCGGGAGGGCGCAGTGGA
GGGAACGGTAGTCGTTGCCGACAGCCAGACGGAAGGCCGGGGGCGGATGCGCCGCGCGTGGGTTTCGCCCGCAGGGGTGA
ATCTCTATTGCTCGATTGTGCTGCGCCCGCCCGTGCCCTCCATCCGCGTGCCGGAGATTCCGCTCGTGGCGGCGGCGGCG
ATCCACGAAGCCGTGACTCAGGAGTGCCCCGGCCTGGCGGCCTTCATCAAGTGGCCGAACGACATCATCACGGGCGGGCG
AAAGCTCTGCGGCATTCTGTGCGAGATGGAGTCCGAACCGGACTGCACACACTTCGTGGTGGTGGGCTTCGGGCTGAACG
TCAACCTCGATCCCGTACCAGAGGAGCTGCGAAAGATCGCCACCTCCATCGCCATCGAGACCGGCTTGCGAATGTCCCGA
GCACGGCTGCTCGCCGCCGTGCTCAACCGCTTCGAGCAGCTCTACCGCGACTGGCTCGAAAAGGAGAACCTGGCGTTCAT
CCTGCCATACCTCGAAGCACACTCCTGGCTGAAAGGGCGCGAGCTGAAAATCGAACAGTTCAACACGGTGCTGACCGGCA
CCGAGGCCGGATTGTCGCCGCAGGGCCACCTCCTCTTGCGCACCGTCGATGGCGCTCTGCTGTCTGTCACTTCGGGCGAG
GCTCACCTGCTTCCCGTCAACCACGAACCGAGATCCGCATGA

Upstream 100 bases:

>100_bases
AGAGAGTTTTTCTTTTCAGATCCAGACTGTCTCCTATCATGCACGGCTTCCTTCACCACCATCTTTTGCCGTTATCGGCC
GCCTCGGGCGTGGAGTCGGT

Downstream 100 bases:

>100_bases
AGTCACGACTGCATCCCGACATTGAAAAGGCCTACGCCGTGCTCGACACCGGCGAGCCGCTCTCGCTCGAACTCGCTTCG
GCGCTGGGGCGGCTTCCGGA

Product: BirA bifunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MNPVTATILQRLAGDGGFVSGGDLCNELQISRSAVWKHIVALRKAGYAIEATSGKGYRLESLTGSPVAEEVAPLLATESF
GRNFIYLESIDSTNLRARASAREGAVEGTVVVADSQTEGRGRMRRAWVSPAGVNLYCSIVLRPPVPSIRVPEIPLVAAAA
IHEAVTQECPGLAAFIKWPNDIITGGRKLCGILCEMESEPDCTHFVVVGFGLNVNLDPVPEELRKIATSIAIETGLRMSR
ARLLAAVLNRFEQLYRDWLEKENLAFILPYLEAHSWLKGRELKIEQFNTVLTGTEAGLSPQGHLLLRTVDGALLSVTSGE
AHLLPVNHEPRSA

Sequences:

>Translated_333_residues
MNPVTATILQRLAGDGGFVSGGDLCNELQISRSAVWKHIVALRKAGYAIEATSGKGYRLESLTGSPVAEEVAPLLATESF
GRNFIYLESIDSTNLRARASAREGAVEGTVVVADSQTEGRGRMRRAWVSPAGVNLYCSIVLRPPVPSIRVPEIPLVAAAA
IHEAVTQECPGLAAFIKWPNDIITGGRKLCGILCEMESEPDCTHFVVVGFGLNVNLDPVPEELRKIATSIAIETGLRMSR
ARLLAAVLNRFEQLYRDWLEKENLAFILPYLEAHSWLKGRELKIEQFNTVLTGTEAGLSPQGHLLLRTVDGALLSVTSGE
AHLLPVNHEPRSA
>Mature_333_residues
MNPVTATILQRLAGDGGFVSGGDLCNELQISRSAVWKHIVALRKAGYAIEATSGKGYRLESLTGSPVAEEVAPLLATESF
GRNFIYLESIDSTNLRARASAREGAVEGTVVVADSQTEGRGRMRRAWVSPAGVNLYCSIVLRPPVPSIRVPEIPLVAAAA
IHEAVTQECPGLAAFIKWPNDIITGGRKLCGILCEMESEPDCTHFVVVGFGLNVNLDPVPEELRKIATSIAIETGLRMSR
ARLLAAVLNRFEQLYRDWLEKENLAFILPYLEAHSWLKGRELKIEQFNTVLTGTEAGLSPQGHLLLRTVDGALLSVTSGE
AHLLPVNHEPRSA

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=313, Percent_Identity=31.629392971246, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36098; Mature: 36098

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPVTATILQRLAGDGGFVSGGDLCNELQISRSAVWKHIVALRKAGYAIEATSGKGYRLE
CCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
SLTGSPVAEEVAPLLATESFGRNFIYLESIDSTNLRARASAREGAVEGTVVVADSQTEGR
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCC
GRMRRAWVSPAGVNLYCSIVLRPPVPSIRVPEIPLVAAAAIHEAVTQECPGLAAFIKWPN
CHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCH
DIITGGRKLCGILCEMESEPDCTHFVVVGFGLNVNLDPVPEELRKIATSIAIETGLRMSR
HHHHCCHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
ARLLAAVLNRFEQLYRDWLEKENLAFILPYLEAHSWLKGRELKIEQFNTVLTGTEAGLSP
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCEEHHHHCEEEECCCCCCCC
QGHLLLRTVDGALLSVTSGEAHLLPVNHEPRSA
CCCEEEEEECCEEEEEECCCEEEEECCCCCCCC
>Mature Secondary Structure
MNPVTATILQRLAGDGGFVSGGDLCNELQISRSAVWKHIVALRKAGYAIEATSGKGYRLE
CCCHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
SLTGSPVAEEVAPLLATESFGRNFIYLESIDSTNLRARASAREGAVEGTVVVADSQTEGR
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEECCCCCCC
GRMRRAWVSPAGVNLYCSIVLRPPVPSIRVPEIPLVAAAAIHEAVTQECPGLAAFIKWPN
CHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCH
DIITGGRKLCGILCEMESEPDCTHFVVVGFGLNVNLDPVPEELRKIATSIAIETGLRMSR
HHHHCCHHHHHHHEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHH
ARLLAAVLNRFEQLYRDWLEKENLAFILPYLEAHSWLKGRELKIEQFNTVLTGTEAGLSP
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCCCEEHHHHCEEEECCCCCCCC
QGHLLLRTVDGALLSVTSGEAHLLPVNHEPRSA
CCCEEEEEECCEEEEEECCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]