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The map label for this gene is bioD

Identifier: 21672889

GI number: 21672889

Start: 46744

End: 47463

Strand: Reverse

Name: bioD

Synonym: CT0048

Alternate gene names: 21672889

Gene position: 47463-46744 (Counterclockwise)

Preceding gene: 21672890

Following gene: 21672888

Centisome position: 2.2

GC content: 62.08

Gene sequence:

>720_bases
TTGCTGTTTCAGAAAGAAAAAGTCATGAAAGGACAGGTACTCGCCATCTCCGGCATCGACACCGGCATCGGCAAAACCGT
CGTCACCGGCCTGCTCGCCCGGTGCTTCGCTGAAACGGGCTGGCGGACAATCACCCAGAAAATCGCACAAACCGGCTGCG
AAGGCGTTTCGGAAGACATCGCCGAACATCGGAAACTGATGGGTATCGACTTGCAGGAGGCCGATCTCGATGGAACGACC
TGCCCATACCTCTTCCGATTTCCGGCCTCGCCACACCTGGCTGCTACAGTGGAGGGGCGCGAGATCGATTTCATGACGAT
CCGCCGCAGCACCTTCAGATTGCAGAAGCTCTACGACCTCGTGCTGCTCGAAGGGGTCGGTGGGCTGCTCGTGCCACTCA
CGCCTGAACTGCTCTTCGCCGACTACGTGCGCGACGCGGGCTACGGCCTCGTGCTGGTCAGCGCCTCGCGGCTCGGCAGC
ATCAACCACACACTCCTCTCGCTCGAAGCCTGTGCAAGGCGCGGCATTCCTGTCCGGGCCATCGTCTACAACCGCTATTT
CGAGGCGGACGAACGTATCGCCGCCAACACGCGCGAAGTGATCGCCGCCGCGCTCAAGCGCTACGGCTTCGGTGAGGCGC
CGGTGATCGATCTCAACACCAGCGGGCTCTCGGCGGAGCCGGGCGACCTGCAACGCATCCTGAACCCTCCGGGCCAATGA

Upstream 100 bases:

>100_bases
GCCGCGCCTGGACGCGCTCGCAGTACCTCGATTTTCTGCAACGCTACCGGTCAGCCTACCCGTCAGGCGAAGGCGTTACG
CTCACCTGGCATCCGGTATA

Downstream 100 bases:

>100_bases
CGATAGACCTCGATTTCGACCGCTGCCACCTCTGGCACCCCTACACCTCGATGGCCGATCCCTTGCCGGTCTGGCCGGTC
AAACGCGCGAGCGGCGTGAT

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MLFQKEKVMKGQVLAISGIDTGIGKTVVTGLLARCFAETGWRTITQKIAQTGCEGVSEDIAEHRKLMGIDLQEADLDGTT
CPYLFRFPASPHLAATVEGREIDFMTIRRSTFRLQKLYDLVLLEGVGGLLVPLTPELLFADYVRDAGYGLVLVSASRLGS
INHTLLSLEACARRGIPVRAIVYNRYFEADERIAANTREVIAAALKRYGFGEAPVIDLNTSGLSAEPGDLQRILNPPGQ

Sequences:

>Translated_239_residues
MLFQKEKVMKGQVLAISGIDTGIGKTVVTGLLARCFAETGWRTITQKIAQTGCEGVSEDIAEHRKLMGIDLQEADLDGTT
CPYLFRFPASPHLAATVEGREIDFMTIRRSTFRLQKLYDLVLLEGVGGLLVPLTPELLFADYVRDAGYGLVLVSASRLGS
INHTLLSLEACARRGIPVRAIVYNRYFEADERIAANTREVIAAALKRYGFGEAPVIDLNTSGLSAEPGDLQRILNPPGQ
>Mature_239_residues
MLFQKEKVMKGQVLAISGIDTGIGKTVVTGLLARCFAETGWRTITQKIAQTGCEGVSEDIAEHRKLMGIDLQEADLDGTT
CPYLFRFPASPHLAATVEGREIDFMTIRRSTFRLQKLYDLVLLEGVGGLLVPLTPELLFADYVRDAGYGLVLVSASRLGS
INHTLLSLEACARRGIPVRAIVYNRYFEADERIAANTREVIAAALKRYGFGEAPVIDLNTSGLSAEPGDLQRILNPPGQ

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=180, Percent_Identity=31.6666666666667, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_CHLTE (Q8KGC0)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660954.1
- ProteinModelPortal:   Q8KGC0
- SMR:   Q8KGC0
- GeneID:   1006466
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0048
- NMPDR:   fig|194439.1.peg.48
- TIGR:   CT0048
- HOGENOM:   HBG650065
- OMA:   IDTGIGK
- ProtClustDB:   PRK00090
- BioCyc:   CTEP194439:CT_0048-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- InterPro:   IPR002586
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: PF01656 CbiA

EC number: =6.3.3.3

Molecular weight: Translated: 26098; Mature: 26098

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFQKEKVMKGQVLAISGIDTGIGKTVVTGLLARCFAETGWRTITQKIAQTGCEGVSEDI
CCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
AEHRKLMGIDLQEADLDGTTCPYLFRFPASPHLAATVEGREIDFMTIRRSTFRLQKLYDL
HHHHHHHCCCCHHCCCCCCCCCEEEECCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
VLLEGVGGLLVPLTPELLFADYVRDAGYGLVLVSASRLGSINHTLLSLEACARRGIPVRA
HHHHCCCCEEECCCHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHHHHHCCCCEEE
IVYNRYFEADERIAANTREVIAAALKRYGFGEAPVIDLNTSGLSAEPGDLQRILNPPGQ
EEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCC
>Mature Secondary Structure
MLFQKEKVMKGQVLAISGIDTGIGKTVVTGLLARCFAETGWRTITQKIAQTGCEGVSEDI
CCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
AEHRKLMGIDLQEADLDGTTCPYLFRFPASPHLAATVEGREIDFMTIRRSTFRLQKLYDL
HHHHHHHCCCCHHCCCCCCCCCEEEECCCCCCEEEEECCCEEEEEEHHHHHHHHHHHHHH
VLLEGVGGLLVPLTPELLFADYVRDAGYGLVLVSASRLGSINHTLLSLEACARRGIPVRA
HHHHCCCCEEECCCHHHHHHHHHHCCCCCEEEEEHHHCCCHHHHHHHHHHHHHCCCCEEE
IVYNRYFEADERIAANTREVIAAALKRYGFGEAPVIDLNTSGLSAEPGDLQRILNPPGQ
EEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12093901