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The map label for this gene is bioA [H]

Identifier: 21672888

GI number: 21672888

Start: 45467

End: 46747

Strand: Reverse

Name: bioA [H]

Synonym: CT0047

Alternate gene names: 21672888

Gene position: 46747-45467 (Counterclockwise)

Preceding gene: 21672889

Following gene: 21672887

Centisome position: 2.17

GC content: 62.84

Gene sequence:

>1281_bases
ATGACGATAGACCTCGATTTCGACCGCTGCCACCTCTGGCACCCCTACACCTCGATGGCCGATCCCTTGCCGGTCTGGCC
GGTCAAACGCGCGAGCGGCGTGATGATCGAGCTGGAGGACGGGCGGAAGCTCATCGACGGCATGTCGTCGTGGTGGGCGG
CGATTCACGGCTACAACCATCCGGTGCTGAACCGCGCCGTGACCGAGCAGCTCGGGCGCATGAGCCACGTCATGTTCGGC
GGCCTGACACACGAACCAGCCATCGAGCTTGGGAAAATCCTGACCAGCCTCTTGCCCGATCCGCTCGACCGCATTTTCTT
CTGCGATTCCGGTTCGGTAGCGGTCGAGGTAGCCATCAAAATGGCCCTGCAATACTGGCTGGCTGCGGGCAAGCCTGGCA
AAAAGCGACTGCTCACCGTGCGTTCCGGCTACCACGGCGATACCTTCATGGCGATGTCGGTCTGCGACCCGGTCACGGGA
ATGCACAGCCTCTTCAGCGGGGCGGTGCCGGAGCAGCTCTTCGTCGAAGCGCCCGCCTGCGGGTTCAACGAGCCGTGGCG
CGAGGAGGCGATTGACAAAATGCGGCAGGCGCTCGAAGATCATGCCAACACTATCGCGGCAGTCATCATCGAGCCGATTG
TGCAGGGCGCAGGCGGAATGCGCTTCTACTCGCCGCACTACCTGCGACGCCTCCGAGAACTGTGCACGGAGCACGGCGTG
CTGCTTATCTTCGACGAAATCGCCACCGGCTTCGGGCGCACCGGCAAGCTGTTCGCGATGGAGTACGCAAGCGTCACACC
TGACATCGTCTGCCTCGGCAAGGCACTCACTGGCGGATACATGACCCTCGCCGCCACCGTCACAACTGGCCACGTCGCCG
ACACGATCTCCGGCGGCAATCCCGGACTTTTCATGCACGGCCCCACCTTCATGGCCAACCCACTCGCCTGCGCGGTCGCC
GTGGCGAGCCTGAAGCTGCTGCTCTCCGGCGACTGGCAATCCACTGTTTGGCGCATCGAACGCCAGCTCGCCGAGGAACT
CGCGCCCTGTACCGGAATGACCGGAGTCCGCGACGTGAGGGTGCTCGGCGCGATCGGCGTGGTGGAACTCGACCGCCCGG
TCGATATGGCGAAAATCCAGCAGGCGTTCGTGGAACGGGGCATCTGGGTCAGGCCATTCGGCAGGTTGGTCTATCTGATG
CCGCCATTCATTATCCGTGACAACGAGCTGACTCGTCTCACGTCAGTAATCTGCGAGGTCATTGGGGCAGAATACAGATG
A

Upstream 100 bases:

>100_bases
AGCGCTACGGCTTCGGTGAGGCGCCGGTGATCGATCTCAACACCAGCGGGCTCTCGGCGGAGCCGGGCGACCTGCAACGC
ATCCTGAACCCTCCGGGCCA

Downstream 100 bases:

>100_bases
AAGTGTCTATTTTTTCTCCTGTCATCCTGTCCATCTTTTGGCGGATCATGCGATAACCTACAACCAGGACTCCTATGACT
AACGCACCCTCTAACGCACC

Product: adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTIDLDFDRCHLWHPYTSMADPLPVWPVKRASGVMIELEDGRKLIDGMSSWWAAIHGYNHPVLNRAVTEQLGRMSHVMFG
GLTHEPAIELGKILTSLLPDPLDRIFFCDSGSVAVEVAIKMALQYWLAAGKPGKKRLLTVRSGYHGDTFMAMSVCDPVTG
MHSLFSGAVPEQLFVEAPACGFNEPWREEAIDKMRQALEDHANTIAAVIIEPIVQGAGGMRFYSPHYLRRLRELCTEHGV
LLIFDEIATGFGRTGKLFAMEYASVTPDIVCLGKALTGGYMTLAATVTTGHVADTISGGNPGLFMHGPTFMANPLACAVA
VASLKLLLSGDWQSTVWRIERQLAEELAPCTGMTGVRDVRVLGAIGVVELDRPVDMAKIQQAFVERGIWVRPFGRLVYLM
PPFIIRDNELTRLTSVICEVIGAEYR

Sequences:

>Translated_426_residues
MTIDLDFDRCHLWHPYTSMADPLPVWPVKRASGVMIELEDGRKLIDGMSSWWAAIHGYNHPVLNRAVTEQLGRMSHVMFG
GLTHEPAIELGKILTSLLPDPLDRIFFCDSGSVAVEVAIKMALQYWLAAGKPGKKRLLTVRSGYHGDTFMAMSVCDPVTG
MHSLFSGAVPEQLFVEAPACGFNEPWREEAIDKMRQALEDHANTIAAVIIEPIVQGAGGMRFYSPHYLRRLRELCTEHGV
LLIFDEIATGFGRTGKLFAMEYASVTPDIVCLGKALTGGYMTLAATVTTGHVADTISGGNPGLFMHGPTFMANPLACAVA
VASLKLLLSGDWQSTVWRIERQLAEELAPCTGMTGVRDVRVLGAIGVVELDRPVDMAKIQQAFVERGIWVRPFGRLVYLM
PPFIIRDNELTRLTSVICEVIGAEYR
>Mature_425_residues
TIDLDFDRCHLWHPYTSMADPLPVWPVKRASGVMIELEDGRKLIDGMSSWWAAIHGYNHPVLNRAVTEQLGRMSHVMFGG
LTHEPAIELGKILTSLLPDPLDRIFFCDSGSVAVEVAIKMALQYWLAAGKPGKKRLLTVRSGYHGDTFMAMSVCDPVTGM
HSLFSGAVPEQLFVEAPACGFNEPWREEAIDKMRQALEDHANTIAAVIIEPIVQGAGGMRFYSPHYLRRLRELCTEHGVL
LIFDEIATGFGRTGKLFAMEYASVTPDIVCLGKALTGGYMTLAATVTTGHVADTISGGNPGLFMHGPTFMANPLACAVAV
ASLKLLLSGDWQSTVWRIERQLAEELAPCTGMTGVRDVRVLGAIGVVELDRPVDMAKIQQAFVERGIWVRPFGRLVYLMP
PFIIRDNELTRLTSVICEVIGAEYR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0161

COG function: function code H; Adenosylmethionine-8-amino-7-oxononanoate aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557809, Length=432, Percent_Identity=30.0925925925926, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI284507298, Length=320, Percent_Identity=32.1875, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI13994255, Length=335, Percent_Identity=28.3582089552239, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI37574042, Length=439, Percent_Identity=25.7403189066059, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI226442709, Length=402, Percent_Identity=26.1194029850746, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI226442705, Length=439, Percent_Identity=25.5125284738041, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI24119277, Length=326, Percent_Identity=26.0736196319018, Blast_Score=83, Evalue=5e-16,
Organism=Escherichia coli, GI1786991, Length=423, Percent_Identity=61.4657210401891, Blast_Score=519, Evalue=1e-148,
Organism=Escherichia coli, GI145693181, Length=321, Percent_Identity=36.4485981308411, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789016, Length=429, Percent_Identity=30.3030303030303, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789759, Length=411, Percent_Identity=29.683698296837, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1788044, Length=399, Percent_Identity=33.0827067669173, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1787560, Length=412, Percent_Identity=28.6407766990291, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786349, Length=324, Percent_Identity=30.8641975308642, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI25144271, Length=428, Percent_Identity=27.1028037383178, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI32564660, Length=427, Percent_Identity=28.1030444964871, Blast_Score=125, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI25144274, Length=229, Percent_Identity=31.4410480349345, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71992977, Length=458, Percent_Identity=22.9257641921397, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17541228, Length=289, Percent_Identity=25.9515570934256, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324386, Length=457, Percent_Identity=44.8577680525164, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323470, Length=411, Percent_Identity=27.007299270073, Blast_Score=130, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6324432, Length=288, Percent_Identity=28.125, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21357415, Length=407, Percent_Identity=28.009828009828, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI161085790, Length=360, Percent_Identity=27.5, Blast_Score=102, Evalue=7e-22,
Organism=Drosophila melanogaster, GI28574759, Length=361, Percent_Identity=27.4238227146814, Blast_Score=101, Evalue=8e-22,
Organism=Drosophila melanogaster, GI21356575, Length=353, Percent_Identity=25.2124645892351, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281366494, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667139, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24667143, Length=289, Percent_Identity=25.9515570934256, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR005814
- InterPro:   IPR005815
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR007197 [H]

Pfam domain/function: PF00202 Aminotran_3; PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 46902; Mature: 46771

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDLDFDRCHLWHPYTSMADPLPVWPVKRASGVMIELEDGRKLIDGMSSWWAAIHGYNH
CEEECCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
PVLNRAVTEQLGRMSHVMFGGLTHEPAIELGKILTSLLPDPLDRIFFCDSGSVAVEVAIK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHH
MALQYWLAAGKPGKKRLLTVRSGYHGDTFMAMSVCDPVTGMHSLFSGAVPEQLFVEAPAC
HHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHCCHHHHHHHHCCCCCHHHEEECCCC
GFNEPWREEAIDKMRQALEDHANTIAAVIIEPIVQGAGGMRFYSPHYLRRLRELCTEHGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCE
LLIFDEIATGFGRTGKLFAMEYASVTPDIVCLGKALTGGYMTLAATVTTGHVADTISGGN
EEEEHHHHHCCCCCCCEEEEEHHCCCCCCEEECHHHCCCEEEEEEEEECCCHHHCCCCCC
PGLFMHGPTFMANPLACAVAVASLKLLLSGDWQSTVWRIERQLAEELAPCTGMTGVRDVR
CCEEEECCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLGAIGVVELDRPVDMAKIQQAFVERGIWVRPFGRLVYLMPPFIIRDNELTRLTSVICEV
HHHCCCEEEECCCCCHHHHHHHHHHCCCEECCHHCEEEECCCEEECCCHHHHHHHHHHHH
IGAEYR
HCCCCC
>Mature Secondary Structure 
TIDLDFDRCHLWHPYTSMADPLPVWPVKRASGVMIELEDGRKLIDGMSSWWAAIHGYNH
EEECCCCCEEEECCHHHCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCC
PVLNRAVTEQLGRMSHVMFGGLTHEPAIELGKILTSLLPDPLDRIFFCDSGSVAVEVAIK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCHHHHHHHH
MALQYWLAAGKPGKKRLLTVRSGYHGDTFMAMSVCDPVTGMHSLFSGAVPEQLFVEAPAC
HHHHHHHHCCCCCCCEEEEEECCCCCCCEEEHHHHCCHHHHHHHHCCCCCHHHEEECCCC
GFNEPWREEAIDKMRQALEDHANTIAAVIIEPIVQGAGGMRFYSPHYLRRLRELCTEHGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCE
LLIFDEIATGFGRTGKLFAMEYASVTPDIVCLGKALTGGYMTLAATVTTGHVADTISGGN
EEEEHHHHHCCCCCCCEEEEEHHCCCCCCEEECHHHCCCEEEEEEEEECCCHHHCCCCCC
PGLFMHGPTFMANPLACAVAVASLKLLLSGDWQSTVWRIERQLAEELAPCTGMTGVRDVR
CCEEEECCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
VLGAIGVVELDRPVDMAKIQQAFVERGIWVRPFGRLVYLMPPFIIRDNELTRLTSVICEV
HHHCCCEEEECCCCCHHHHHHHHHHCCCEECCHHCEEEECCCEEECCCHHHHHHHHHHHH
IGAEYR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]