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The map label for this gene is murE

Identifier: 21672880

GI number: 21672880

Start: 36797

End: 38323

Strand: Reverse

Name: murE

Synonym: CT0039

Alternate gene names: 21672880

Gene position: 38323-36797 (Counterclockwise)

Preceding gene: 21672881

Following gene: 21672879

Centisome position: 1.78

GC content: 65.88

Gene sequence:

>1527_bases
ATGAAGGAAATCCGCGAAGGAGCGCCGGGTGCGCAGCTCGACGACCTGGTCGCCGCTCTCGGCGCGCTGGCTGAACGCCG
GGGCGGCGACGGAGCGCGAGCCGTCATCACGGGCGTGACCTGCGACTCGCGGGCGGTCACGCCGGGTGCGCTCTTCGTGG
CGGTGCGTGGCCTCGTCGCCGACGGCCACCACTTCATCGGCGCGGCCATCGAAGCGGGCGCGGTGGCGGTGGCGTGCGAA
GAGCTTCCGGCTGCGTACAGCGATTCGGTCACCTGGCTCGTCGTGCCGGACGCCCGAAAGGCGCTCGCCGAACTCTCGAA
AGCCTTTTACGGCAACGCTTCGGACAAGCTCATGCTCATCGGCGTAACCGGCACCAACGGCAAGACCACCACCGCGCGGC
TCGTCACCTCGATGCTCAACGCATCGGGCGTCGCCGCCGGATACATCGGCACCGGCCTCTGCCGCATCGGCAATCACGAC
ATTCCGCTCGAACGCACCACTCCCGAGCCGAACAGGCTTCACGATCTTTTCCGCCAGATGGTCGATGCCGGATGCCGGGC
GGCGGTGATGGAGGTCTCCTCCCACTCACTGGTGCTCGACAGAGTGCATGGCCTCTTCTTCCGCGCCGCCGTCTTCACCA
ACCTCACGCCGGAGCACCTCGACTTTCACGAAACGATGGAGGAGTACGCCGAAGCCAAGCGCCTGCTCTTCGACCAGCTC
AACGCCGAGGGCTTCGCCGTCATCAACGCCGACGATCCCCGTGCGGAGTTCATGGCCGCCAGGCTTGCGCCGGAGCGCGT
TTTCTGCTGCTCGACCGGCGATAATACATCGCTCTGCGATCCGGCTCGTCGCTTCCATGCGGTCATCACCGCTTCGACGG
TCGAAGGCTCGAAAGCCGATGTCACGTTTGACGGACAGTCGATGGCGATGCAGGTTCCTCTGCCGGGCGCGTACAACGTG
ATGAACATGCTCGAAGCGTTCACGGTCGGCGTCGGCCTCGGCATCGATCCCGCTACCGCGCTTCGCAGCCTCGCCGCCGC
CGATGCGATCGCGGGCCGAATGGAGCGCATCTGGAGCCGCGACCGCAGCCGCTGCGCCGTGGTCGATTACGCTCACACGC
CTGACGCCTTGCAAAAGGCGCTCGAAGCCCTGCGCGCGGTGACGCCTGCCGACGCGAAGCTCGCGGTGGTCTTTGGCTGT
GGTGGCAACCGCGACCGGCAGAAGCGCCCCGAAATGGGCCGCATCGCCGCCGAACTGGCCGACAGGGTGATTCTCACTTC
CGACAATCCACGGGACGAAAATCCGGAAGCGATTCTCGACGAGGTCGAGGCAGGCATGGCGGGCCGGGTGCATCTACGCA
TCGCCGACCGTGCCGAGGCGATCCGCCGGGCGGTCGAGCAGCTCGGTGCGGGCGATATTCTGCTGGTGGCAGGAAAAGGG
CACGAAGCGTATCAGGAGATCAGGGGCGTGAAGCACCACTTTTCCGACCGCGAGTGTCTGGAGGCATGTTTTGCGCAAAT
GAAATGA

Upstream 100 bases:

>100_bases
GCGGCGGCAGCGTGACCTCGCAGAGCATCGCGCCCGGCACGAAGGTACAGAAAGAAACAACGGTCAGGGTTCGCCTGGCG
AAAAGGGTTCAGAACAAGTA

Downstream 100 bases:

>100_bases
GTTAACGGAAGTCAGGGAGTGAAGATGAAAGGAGCGCTGATGTTCAGCGATTTCGAGCGGGCGGGAACCGTCGTGGCCCG
TGACGTTGGCGAGGGGTACC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MKEIREGAPGAQLDDLVAALGALAERRGGDGARAVITGVTCDSRAVTPGALFVAVRGLVADGHHFIGAAIEAGAVAVACE
ELPAAYSDSVTWLVVPDARKALAELSKAFYGNASDKLMLIGVTGTNGKTTTARLVTSMLNASGVAAGYIGTGLCRIGNHD
IPLERTTPEPNRLHDLFRQMVDAGCRAAVMEVSSHSLVLDRVHGLFFRAAVFTNLTPEHLDFHETMEEYAEAKRLLFDQL
NAEGFAVINADDPRAEFMAARLAPERVFCCSTGDNTSLCDPARRFHAVITASTVEGSKADVTFDGQSMAMQVPLPGAYNV
MNMLEAFTVGVGLGIDPATALRSLAAADAIAGRMERIWSRDRSRCAVVDYAHTPDALQKALEALRAVTPADAKLAVVFGC
GGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEVEAGMAGRVHLRIADRAEAIRRAVEQLGAGDILLVAGKG
HEAYQEIRGVKHHFSDRECLEACFAQMK

Sequences:

>Translated_508_residues
MKEIREGAPGAQLDDLVAALGALAERRGGDGARAVITGVTCDSRAVTPGALFVAVRGLVADGHHFIGAAIEAGAVAVACE
ELPAAYSDSVTWLVVPDARKALAELSKAFYGNASDKLMLIGVTGTNGKTTTARLVTSMLNASGVAAGYIGTGLCRIGNHD
IPLERTTPEPNRLHDLFRQMVDAGCRAAVMEVSSHSLVLDRVHGLFFRAAVFTNLTPEHLDFHETMEEYAEAKRLLFDQL
NAEGFAVINADDPRAEFMAARLAPERVFCCSTGDNTSLCDPARRFHAVITASTVEGSKADVTFDGQSMAMQVPLPGAYNV
MNMLEAFTVGVGLGIDPATALRSLAAADAIAGRMERIWSRDRSRCAVVDYAHTPDALQKALEALRAVTPADAKLAVVFGC
GGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEVEAGMAGRVHLRIADRAEAIRRAVEQLGAGDILLVAGKG
HEAYQEIRGVKHHFSDRECLEACFAQMK
>Mature_508_residues
MKEIREGAPGAQLDDLVAALGALAERRGGDGARAVITGVTCDSRAVTPGALFVAVRGLVADGHHFIGAAIEAGAVAVACE
ELPAAYSDSVTWLVVPDARKALAELSKAFYGNASDKLMLIGVTGTNGKTTTARLVTSMLNASGVAAGYIGTGLCRIGNHD
IPLERTTPEPNRLHDLFRQMVDAGCRAAVMEVSSHSLVLDRVHGLFFRAAVFTNLTPEHLDFHETMEEYAEAKRLLFDQL
NAEGFAVINADDPRAEFMAARLAPERVFCCSTGDNTSLCDPARRFHAVITASTVEGSKADVTFDGQSMAMQVPLPGAYNV
MNMLEAFTVGVGLGIDPATALRSLAAADAIAGRMERIWSRDRSRCAVVDYAHTPDALQKALEALRAVTPADAKLAVVFGC
GGNRDRQKRPEMGRIAAELADRVILTSDNPRDENPEAILDEVEAGMAGRVHLRIADRAEAIRRAVEQLGAGDILLVAGKG
HEAYQEIRGVKHHFSDRECLEACFAQMK

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=476, Percent_Identity=40.7563025210084, Blast_Score=238, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_CHLTE (Q8KGC9)

Other databases:

- EMBL:   AE006470
- RefSeq:   NP_660945.1
- ProteinModelPortal:   Q8KGC9
- SMR:   Q8KGC9
- GeneID:   1006457
- GenomeReviews:   AE006470_GR
- KEGG:   cte:CT0039
- NMPDR:   fig|194439.1.peg.39
- TIGR:   CT0039
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   CLSK637122
- BioCyc:   CTEP194439:CT_0039-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54302; Mature: 54302

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: BINDING 43-43 BINDING 193-193 BINDING 201-201 BINDING 404-404 BINDING 478-478 BINDING 482-482

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIREGAPGAQLDDLVAALGALAERRGGDGARAVITGVTCDSRAVTPGALFVAVRGLVA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
DGHHFIGAAIEAGAVAVACEELPAAYSDSVTWLVVPDARKALAELSKAFYGNASDKLMLI
CCCHHHHHHHHCCHHEEEHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEE
GVTGTNGKTTTARLVTSMLNASGVAAGYIGTGLCRIGNHDIPLERTTPEPNRLHDLFRQM
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHH
VDAGCRAAVMEVSSHSLVLDRVHGLFFRAAVFTNLTPEHLDFHETMEEYAEAKRLLFDQL
HHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHC
NAEGFAVINADDPRAEFMAARLAPERVFCCSTGDNTSLCDPARRFHAVITASTVEGSKAD
CCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEECCCCCCE
VTFDGQSMAMQVPLPGAYNVMNMLEAFTVGVGLGIDPATALRSLAAADAIAGRMERIWSR
EEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
DRSRCAVVDYAHTPDALQKALEALRAVTPADAKLAVVFGCGGNRDRQKRPEMGRIAAELA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHH
DRVILTSDNPRDENPEAILDEVEAGMAGRVHLRIADRAEAIRRAVEQLGAGDILLVAGKG
CEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCC
HEAYQEIRGVKHHFSDRECLEACFAQMK
HHHHHHHHHHHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MKEIREGAPGAQLDDLVAALGALAERRGGDGARAVITGVTCDSRAVTPGALFVAVRGLVA
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHH
DGHHFIGAAIEAGAVAVACEELPAAYSDSVTWLVVPDARKALAELSKAFYGNASDKLMLI
CCCHHHHHHHHCCHHEEEHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCEEEE
GVTGTNGKTTTARLVTSMLNASGVAAGYIGTGLCRIGNHDIPLERTTPEPNRLHDLFRQM
EEECCCCCHHHHHHHHHHHHCCCCCHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHH
VDAGCRAAVMEVSSHSLVLDRVHGLFFRAAVFTNLTPEHLDFHETMEEYAEAKRLLFDQL
HHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHC
NAEGFAVINADDPRAEFMAARLAPERVFCCSTGDNTSLCDPARRFHAVITASTVEGSKAD
CCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHEEEEEEEECCCCCCE
VTFDGQSMAMQVPLPGAYNVMNMLEAFTVGVGLGIDPATALRSLAAADAIAGRMERIWSR
EEECCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHC
DRSRCAVVDYAHTPDALQKALEALRAVTPADAKLAVVFGCGGNRDRQKRPEMGRIAAELA
CCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHCCCHHHHHHHHHH
DRVILTSDNPRDENPEAILDEVEAGMAGRVHLRIADRAEAIRRAVEQLGAGDILLVAGKG
CEEEEECCCCCCCCHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCC
HEAYQEIRGVKHHFSDRECLEACFAQMK
HHHHHHHHHHHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12093901