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The map label for this gene is otsA [H]

Identifier: 13488444

GI number: 13488444

Start: 63112

End: 63939

Strand: Direct

Name: otsA [H]

Synonym: mlr9595

Alternate gene names: 13488444

Gene position: 63112-63939 (Clockwise)

Preceding gene: 13488443

Following gene: 13488448

Centisome position: 30.3

GC content: 61.84

Gene sequence:

>828_bases
GTGCTGGGTTTCCAGACCGCGACGGACGTAACGAATTTTCGGCGCTCCGTTCGGGCGACAGGTTCGACCACCTTCGACAT
CAACGGCGCGGCCAAAAGCAATGGCCGAACCGTCCTGTCGAGGAGCTTTCCGATAGGCATCGATGTCGACGCCTTCGCAC
GCATGGCTAACGATGCGGCGAGTGATGTCCAGATCGATTCCATGCGCCGCCAGATTCTTGGTCTCAAGCAGATCATCGGC
GTCGACAGGCTAGACTACTCCAAAGGGCTGCCCGGCCGCATCCAGGCCTTTGCGCGCCTTCTCGAAAGGCACCCCGAACA
CGAGCGGACGGTAACCTATCTGCAGATAGCGCCGCCCACGCGAGAAGAGGTCGGAGCGTATGCGGAAATACGCGCGCAGC
TCGAGGCAACCGCTGGCTCCGTGAACGGAAAATACGCGGACCTGACCTGGACGCCGATCCGCTACATCCACCGCACCGTT
CCGCGCTCGCGGCTGGCCGGTCTGTTGCGGGCCAGTCAGGTCGGGCTGGTCGCGCCCCTGCGTGATGGAATGAACCTCGT
CGCAAAGGAATATATCGCCGCCCAGGATCCAGCCGATCCCGGGGTCCTCGTATTGTCGCGTTTTGCAGGCGCGGCTGAAG
AGCTTGCGCACTCCTTGATCGTTAACCCCTATGTGGTCGACGAGGTTGCTGACGCGATAGCAAAAGCGCTGGGCATGCCG
CTCGAGGAAAGAATTGCAAGGCACGGTGCCCTGATCGAGCGCATCCGGCGCAATGACGCGGCACAATGGCGGAAGTCATT
TCTCGAGGCGCTGGCGGACAGCAGCTAG

Upstream 100 bases:

>100_bases
GCTGCGGAGTTGCGGCGCCTCGGATTCGGAAATCGCATCGGTTTCTTCCTCCATATCTCCATGGCCAGGCGCAGCAACAA
ACAGGTCCGGCGGCGGGAAC

Downstream 100 bases:

>100_bases
CGTGGCTGGCAACGACCGACTGGATAGCCTCATCGCGCACAGGTTCCTCTGGTGAGATTTTCTTCAGCGGCATGAAACGA
GCGCCATCTTGCGACGTTGC

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MLGFQTATDVTNFRRSVRATGSTTFDINGAAKSNGRTVLSRSFPIGIDVDAFARMANDAASDVQIDSMRRQILGLKQIIG
VDRLDYSKGLPGRIQAFARLLERHPEHERTVTYLQIAPPTREEVGAYAEIRAQLEATAGSVNGKYADLTWTPIRYIHRTV
PRSRLAGLLRASQVGLVAPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEELAHSLIVNPYVVDEVADAIAKALGMP
LEERIARHGALIERIRRNDAAQWRKSFLEALADSS

Sequences:

>Translated_275_residues
MLGFQTATDVTNFRRSVRATGSTTFDINGAAKSNGRTVLSRSFPIGIDVDAFARMANDAASDVQIDSMRRQILGLKQIIG
VDRLDYSKGLPGRIQAFARLLERHPEHERTVTYLQIAPPTREEVGAYAEIRAQLEATAGSVNGKYADLTWTPIRYIHRTV
PRSRLAGLLRASQVGLVAPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEELAHSLIVNPYVVDEVADAIAKALGMP
LEERIARHGALIERIRRNDAAQWRKSFLEALADSS
>Mature_275_residues
MLGFQTATDVTNFRRSVRATGSTTFDINGAAKSNGRTVLSRSFPIGIDVDAFARMANDAASDVQIDSMRRQILGLKQIIG
VDRLDYSKGLPGRIQAFARLLERHPEHERTVTYLQIAPPTREEVGAYAEIRAQLEATAGSVNGKYADLTWTPIRYIHRTV
PRSRLAGLLRASQVGLVAPLRDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEELAHSLIVNPYVVDEVADAIAKALGMP
LEERIARHGALIERIRRNDAAQWRKSFLEALADSS

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=271, Percent_Identity=46.1254612546125, Blast_Score=243, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI71987012, Length=295, Percent_Identity=29.4915254237288, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115533560, Length=231, Percent_Identity=27.7056277056277, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI115533558, Length=231, Percent_Identity=27.7056277056277, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6319602, Length=271, Percent_Identity=36.1623616236162, Blast_Score=183, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320279, Length=249, Percent_Identity=27.710843373494, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323537, Length=271, Percent_Identity=26.9372693726937, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323917, Length=221, Percent_Identity=23.9819004524887, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI19920676, Length=273, Percent_Identity=41.025641025641, Blast_Score=208, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 30103; Mature: 30103

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGFQTATDVTNFRRSVRATGSTTFDINGAAKSNGRTVLSRSFPIGIDVDAFARMANDAA
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCCHH
SDVQIDSMRRQILGLKQIIGVDRLDYSKGLPGRIQAFARLLERHPEHERTVTYLQIAPPT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
REEVGAYAEIRAQLEATAGSVNGKYADLTWTPIRYIHRTVPRSRLAGLLRASQVGLVAPL
HHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
RDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEELAHSLIVNPYVVDEVADAIAKALGMP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
LEERIARHGALIERIRRNDAAQWRKSFLEALADSS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLGFQTATDVTNFRRSVRATGSTTFDINGAAKSNGRTVLSRSFPIGIDVDAFARMANDAA
CCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCCCCHHHHHHHHCCHH
SDVQIDSMRRQILGLKQIIGVDRLDYSKGLPGRIQAFARLLERHPEHERTVTYLQIAPPT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCC
REEVGAYAEIRAQLEATAGSVNGKYADLTWTPIRYIHRTVPRSRLAGLLRASQVGLVAPL
HHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHH
RDGMNLVAKEYIAAQDPADPGVLVLSRFAGAAEELAHSLIVNPYVVDEVADAIAKALGMP
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
LEERIARHGALIERIRRNDAAQWRKSFLEALADSS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA