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The map label for this gene is cysK2 [H]

Identifier: 13488401

GI number: 13488401

Start: 28202

End: 29275

Strand: Direct

Name: cysK2 [H]

Synonym: mlr9541

Alternate gene names: 13488401

Gene position: 28202-29275 (Clockwise)

Preceding gene: 13488400

Following gene: 13488404

Centisome position: 13.54

GC content: 63.04

Gene sequence:

>1074_bases
ATGGTGAGCCCCGCAGTGGACCATTTCAAGGACCTCGAGACCCCGCGCCTTGCGCTGTTGGCGCCCAATCTCGTGGCCGC
TGCTTTCCCGCTGATGAAGCTGATGCCGGCCCGCTACATTCTGGATCGGGCAGCGTCGAATGGCGAATTGAAGACCGGCT
CCCATATTGTCGAGACGACGTCCGGCACATTTGGAATGGCGGTGGCGCTGCTGGCGGCGGCGCGTGACTATCGCCTTACG
CTCGTCACCGCTTCAACGCTGATCGATGCCAAATACCGAGCCCGCCTCGAGCGCATCGGCGCGACGGTGATCGCCCTTGA
CGATCCCCGCGGCGATGGCAACCAACCGGGCCGGCTGGAGCGCCTCCAGGAAATCCTCGCCAAGGAGCCGGATGGCTTCT
GGACTCGCCAATACGATAGTCCGGGTAATTGGCTTGCCTATGCGCGGCTGGCCGAACTCTTGATCCGCGCAATCGGCAAG
GTTGACTGCCTGGTCGGCTGCGTCGGCACGGGCGGCTCGCTATGCGGCACCGCGTCATTTTTGCGAACTGTCTTTCCCCA
TCTGACGGTGTTTGCCGTCGATACCCATCGCAGCATTTTGTTCGGGCAGCCTGTGGGCAGGCGCATGCTGCGAGGCTTGG
GCAACAGCGTTCTGCCGAAAAACGTCAGGCATGAGCTTGTCGACGAAATCCATTGGGTTGGCGCCTATCCCGCATATATG
ACTGCGCACCGTCTGCTGCGCGAGCACGGCCTCTTCATGGGGCCGACCAGCGGCGCCGCCGCCTTGGTGGCGCAATGGGT
TGCAACGACCCTTCCCGACGCGCAGGTGGCGGTCATCATGCCTGACGAAGGCCATCGTCATGCCGAGACTGTCTACAATG
ACGAATGGCTCAGCGCCCTGCCTGGCTGGCCCTGCAAGGAGCTGTCCGAGCCGAGGACGCTGACCACCATCGCACCGGCC
GCAGAGACGCAATGGACGCGCTTTCTTTGGTTGCGTCGCAGCCTCGGCGATGTGCTGAAAACGCAGGCAGCAGGCGAAGT
GCCACCGGCTGTTGGCGCCGCGGAAAATCTATGA

Upstream 100 bases:

>100_bases
CCCCGAACCTGAAGCGGCGGGCAGCTCTGGTGGCCCAGCAGATAAGACGTGCGGGCTTCAAGGATGTCGAGGTCCATCTG
GTCAATGCGGAGGGCTGGCC

Downstream 100 bases:

>100_bases
AACGAGATCCAGCCCGTTCTTGTCGAGCCAGCGCCAGGCGTCGTCCGTCGTCCGGAAGATCTGATCGTGTTCGTTCGATC
CGGCGGACCGATCGAGGTCC

Product: cysteine synthase

Products: @CYST01txt*L-cysteine!; acetate; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MVSPAVDHFKDLETPRLALLAPNLVAAAFPLMKLMPARYILDRAASNGELKTGSHIVETTSGTFGMAVALLAAARDYRLT
LVTASTLIDAKYRARLERIGATVIALDDPRGDGNQPGRLERLQEILAKEPDGFWTRQYDSPGNWLAYARLAELLIRAIGK
VDCLVGCVGTGGSLCGTASFLRTVFPHLTVFAVDTHRSILFGQPVGRRMLRGLGNSVLPKNVRHELVDEIHWVGAYPAYM
TAHRLLREHGLFMGPTSGAAALVAQWVATTLPDAQVAVIMPDEGHRHAETVYNDEWLSALPGWPCKELSEPRTLTTIAPA
AETQWTRFLWLRRSLGDVLKTQAAGEVPPAVGAAENL

Sequences:

>Translated_357_residues
MVSPAVDHFKDLETPRLALLAPNLVAAAFPLMKLMPARYILDRAASNGELKTGSHIVETTSGTFGMAVALLAAARDYRLT
LVTASTLIDAKYRARLERIGATVIALDDPRGDGNQPGRLERLQEILAKEPDGFWTRQYDSPGNWLAYARLAELLIRAIGK
VDCLVGCVGTGGSLCGTASFLRTVFPHLTVFAVDTHRSILFGQPVGRRMLRGLGNSVLPKNVRHELVDEIHWVGAYPAYM
TAHRLLREHGLFMGPTSGAAALVAQWVATTLPDAQVAVIMPDEGHRHAETVYNDEWLSALPGWPCKELSEPRTLTTIAPA
AETQWTRFLWLRRSLGDVLKTQAAGEVPPAVGAAENL
>Mature_357_residues
MVSPAVDHFKDLETPRLALLAPNLVAAAFPLMKLMPARYILDRAASNGELKTGSHIVETTSGTFGMAVALLAAARDYRLT
LVTASTLIDAKYRARLERIGATVIALDDPRGDGNQPGRLERLQEILAKEPDGFWTRQYDSPGNWLAYARLAELLIRAIGK
VDCLVGCVGTGGSLCGTASFLRTVFPHLTVFAVDTHRSILFGQPVGRRMLRGLGNSVLPKNVRHELVDEIHWVGAYPAYM
TAHRLLREHGLFMGPTSGAAALVAQWVATTLPDAQVAVIMPDEGHRHAETVYNDEWLSALPGWPCKELSEPRTLTTIAPA
AETQWTRFLWLRRSLGDVLKTQAAGEVPPAVGAAENL

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI295821200, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI4557415, Length=274, Percent_Identity=29.1970802919708, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1788754, Length=261, Percent_Identity=29.8850574712644, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI2367138, Length=282, Percent_Identity=28.7234042553192, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI32566674, Length=237, Percent_Identity=30.8016877637131, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17562970, Length=237, Percent_Identity=30.8016877637131, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17561720, Length=236, Percent_Identity=31.7796610169492, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32566672, Length=180, Percent_Identity=30.5555555555556, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115535073, Length=294, Percent_Identity=28.5714285714286, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535051, Length=233, Percent_Identity=27.4678111587983, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147552, Length=270, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17534315, Length=277, Percent_Identity=28.5198555956679, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=292, Percent_Identity=23.972602739726, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24643623, Length=270, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI20129101, Length=270, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47 [C]

Molecular weight: Translated: 38886; Mature: 38886

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPAVDHFKDLETPRLALLAPNLVAAAFPLMKLMPARYILDRAASNGELKTGSHIVETT
CCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
SGTFGMAVALLAAARDYRLTLVTASTLIDAKYRARLERIGATVIALDDPRGDGNQPGRLE
CCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
RLQEILAKEPDGFWTRQYDSPGNWLAYARLAELLIRAIGKVDCLVGCVGTGGSLCGTASF
HHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
LRTVFPHLTVFAVDTHRSILFGQPVGRRMLRGLGNSVLPKNVRHELVDEIHWVGAYPAYM
HHHHHCCCEEEEEECCCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
TAHRLLREHGLFMGPTSGAAALVAQWVATTLPDAQVAVIMPDEGHRHAETVYNDEWLSAL
HHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHHHHHC
PGWPCKELSEPRTLTTIAPAAETQWTRFLWLRRSLGDVLKTQAAGEVPPAVGAAENL
CCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVSPAVDHFKDLETPRLALLAPNLVAAAFPLMKLMPARYILDRAASNGELKTGSHIVETT
CCCCHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
SGTFGMAVALLAAARDYRLTLVTASTLIDAKYRARLERIGATVIALDDPRGDGNQPGRLE
CCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHH
RLQEILAKEPDGFWTRQYDSPGNWLAYARLAELLIRAIGKVDCLVGCVGTGGSLCGTASF
HHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHH
LRTVFPHLTVFAVDTHRSILFGQPVGRRMLRGLGNSVLPKNVRHELVDEIHWVGAYPAYM
HHHHHCCCEEEEEECCCCEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
TAHRLLREHGLFMGPTSGAAALVAQWVATTLPDAQVAVIMPDEGHRHAETVYNDEWLSAL
HHHHHHHHCCCEECCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHCCHHHHHC
PGWPCKELSEPRTLTTIAPAAETQWTRFLWLRRSLGDVLKTQAAGEVPPAVGAAENL
CCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} 0.7 {L-Ser}} [C]

Substrates: O-acetyl-L-serine; L-serine; acetyl-CoA; H2S [C]

Specific reaction: O-acetyl-L-serine + H2S = @CYST01.txt*L-cysteine! + acetate L-serine + acetyl-CoA + H2S = L-cysteine + Coenzyme A + acetate [C]

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430 [H]