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The map label for this gene is cheR [C]

Identifier: 13488381

GI number: 13488381

Start: 9905

End: 11146

Strand: Reverse

Name: cheR [C]

Synonym: mll9515

Alternate gene names: 13488381

Gene position: 11146-9905 (Counterclockwise)

Preceding gene: 13488382

Following gene: 13488380

Centisome position: 5.35

GC content: 59.1

Gene sequence:

>1242_bases
GTGACCGGCGCAATAGAAACGCTCCTGACGGAAACCATCGGACTGGACAGTACCAGCGTTGGTCCGACAGTGGTCCGTCA
GGCGATCAAAGATCGGATGGCAGCATGCCGGATAACCAACCCTCAGGCCTATAGGGAACACTTGACCGGTTCCTCGCAGG
AGCTGCAGGAGCTGATCAATGCGGTGGTGATCCCGGAGACCTGGTTCTTTCGCGACCGAGAAGCGTTCACGATGCTGGCG
CGGCATGCGCGCGTGCATAAGCGTGCTCAGTCGATAAAAGTCTTGAGCCTGCCATGTTCGACCGGCGAAGAGGCTTATTC
GGTCGCCATGGCCATGTTCGATGCCGGCTTTGAGGCGCACGAGTTCAAGATCGATGGCGTGGACGTCAGCAGCCGAAATA
TCGCGACGGCTGAACGCGCCATCTATGGGCGCAATTCGTTTCGCGGGAGCCATCTCGAATTCAAGGATCGCTATTTCGAA
GCGGCCGAGGGCGGGTTGCGGCCGATTGCTGGGGTGCTGAAACAGGTTCGTTTCAGGGTAGGGAACCTTTTTGGCTCTCG
CGCGTCTTTCGGGCAAGAGGTCTATGACATCCTGCTTTGCCGCAATTTGCTGATCTATTTCAGTCGAGAACTGCAGGATC
GCGCATTGATCTGCCTGAAGGACCTGCTGGCCAAAGACGGACTTCTTCTGGTCGGTCCGGCGGAAACAGCACTTCCAAGG
TTGCATGGCTTTATTCCCGCCGACTGGCCATTGGCCTTTGCCTTCCTGAAATCGGAGCCCGTCAAAACCACGGCACCGAA
AGTTTCGGCCGCCATCCCGGTGCGGACGCCAACCCCGAAGGTCAACAAGAGCGAGCCTCTTCTTCCGTTCAGACCGACAA
TTGGCAATGAAGGCCGTAGGCAGGCTCCGGCCAAGCCGCTTGCACAGGCCTCGGAAAGCCTCATCGCGGTTGAACGGATC
GCCAATGCAGGACGGGTAAAGGAGGCGCAGCAGGTCGCCCTGGCGCATCTCAAGGAGTTCGGTCCTTCCGCGGAAATCTT
CTACCTGCTGGGCCTCGTCCAGGATGCGGATGGTGCTGCACCCGAAGCCGCGCAAAGTTATCGCAAGGCGCTCTATCTCG
CGCCAAATCATCGAGAAGCTCTTGTCCATCTGGCCTTGCTGCTTAGAAAGCAAGGCGACCATACCGGCGCCGAGGCGCTG
GCCGGCCGGCTGGGTCGGGTCCAGAAAAGGAGCGGCAGCTGA

Upstream 100 bases:

>100_bases
CTTCCGATGGTTCGGAGATCATTCAGTGGGTCAAAGTGGAAGCGCTGCTTTCGGAAGAGATTCGTACCGCATTGCTCCGC
CAGGCCGCGATGGGTGATCA

Downstream 100 bases:

>100_bases
TGGCGGCTTCGACAAGGATCGCTCAGCCGGTGGAGGAGATCGAAATCGACGACTGCTGGCGGCAAATAGGCGTACGGGGT
GACAAATCATGTCCGCTACT

Product: methyltransferase

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MTGAIETLLTETIGLDSTSVGPTVVRQAIKDRMAACRITNPQAYREHLTGSSQELQELINAVVIPETWFFRDREAFTMLA
RHARVHKRAQSIKVLSLPCSTGEEAYSVAMAMFDAGFEAHEFKIDGVDVSSRNIATAERAIYGRNSFRGSHLEFKDRYFE
AAEGGLRPIAGVLKQVRFRVGNLFGSRASFGQEVYDILLCRNLLIYFSRELQDRALICLKDLLAKDGLLLVGPAETALPR
LHGFIPADWPLAFAFLKSEPVKTTAPKVSAAIPVRTPTPKVNKSEPLLPFRPTIGNEGRRQAPAKPLAQASESLIAVERI
ANAGRVKEAQQVALAHLKEFGPSAEIFYLLGLVQDADGAAPEAAQSYRKALYLAPNHREALVHLALLLRKQGDHTGAEAL
AGRLGRVQKRSGS

Sequences:

>Translated_413_residues
MTGAIETLLTETIGLDSTSVGPTVVRQAIKDRMAACRITNPQAYREHLTGSSQELQELINAVVIPETWFFRDREAFTMLA
RHARVHKRAQSIKVLSLPCSTGEEAYSVAMAMFDAGFEAHEFKIDGVDVSSRNIATAERAIYGRNSFRGSHLEFKDRYFE
AAEGGLRPIAGVLKQVRFRVGNLFGSRASFGQEVYDILLCRNLLIYFSRELQDRALICLKDLLAKDGLLLVGPAETALPR
LHGFIPADWPLAFAFLKSEPVKTTAPKVSAAIPVRTPTPKVNKSEPLLPFRPTIGNEGRRQAPAKPLAQASESLIAVERI
ANAGRVKEAQQVALAHLKEFGPSAEIFYLLGLVQDADGAAPEAAQSYRKALYLAPNHREALVHLALLLRKQGDHTGAEAL
AGRLGRVQKRSGS
>Mature_412_residues
TGAIETLLTETIGLDSTSVGPTVVRQAIKDRMAACRITNPQAYREHLTGSSQELQELINAVVIPETWFFRDREAFTMLAR
HARVHKRAQSIKVLSLPCSTGEEAYSVAMAMFDAGFEAHEFKIDGVDVSSRNIATAERAIYGRNSFRGSHLEFKDRYFEA
AEGGLRPIAGVLKQVRFRVGNLFGSRASFGQEVYDILLCRNLLIYFSRELQDRALICLKDLLAKDGLLLVGPAETALPRL
HGFIPADWPLAFAFLKSEPVKTTAPKVSAAIPVRTPTPKVNKSEPLLPFRPTIGNEGRRQAPAKPLAQASESLIAVERIA
NAGRVKEAQQVALAHLKEFGPSAEIFYLLGLVQDADGAAPEAAQSYRKALYLAPNHREALVHLALLLRKQGDHTGAEALA
GRLGRVQKRSGS

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=190, Percent_Identity=32.1052631578947, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80

Molecular weight: Translated: 45275; Mature: 45143

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50005 TPR ; PS50293 TPR_REGION ; PS50123 CHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGAIETLLTETIGLDSTSVGPTVVRQAIKDRMAACRITNPQAYREHLTGSSQELQELIN
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
AVVIPETWFFRDREAFTMLARHARVHKRAQSIKVLSLPCSTGEEAYSVAMAMFDAGFEAH
HEECCCHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
EFKIDGVDVSSRNIATAERAIYGRNSFRGSHLEFKDRYFEAAEGGLRPIAGVLKQVRFRV
EEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GNLFGSRASFGQEVYDILLCRNLLIYFSRELQDRALICLKDLLAKDGLLLVGPAETALPR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
LHGFIPADWPLAFAFLKSEPVKTTAPKVSAAIPVRTPTPKVNKSEPLLPFRPTIGNEGRR
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
QAPAKPLAQASESLIAVERIANAGRVKEAQQVALAHLKEFGPSAEIFYLLGLVQDADGAA
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PEAAQSYRKALYLAPNHREALVHLALLLRKQGDHTGAEALAGRLGRVQKRSGS
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TGAIETLLTETIGLDSTSVGPTVVRQAIKDRMAACRITNPQAYREHLTGSSQELQELIN
CCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
AVVIPETWFFRDREAFTMLARHARVHKRAQSIKVLSLPCSTGEEAYSVAMAMFDAGFEAH
HEECCCHHCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCCCC
EFKIDGVDVSSRNIATAERAIYGRNSFRGSHLEFKDRYFEAAEGGLRPIAGVLKQVRFRV
EEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHH
GNLFGSRASFGQEVYDILLCRNLLIYFSRELQDRALICLKDLLAKDGLLLVGPAETALPR
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHH
LHGFIPADWPLAFAFLKSEPVKTTAPKVSAAIPVRTPTPKVNKSEPLLPFRPTIGNEGRR
HHCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
QAPAKPLAQASESLIAVERIANAGRVKEAQQVALAHLKEFGPSAEIFYLLGLVQDADGAA
CCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
PEAAQSYRKALYLAPNHREALVHLALLLRKQGDHTGAEALAGRLGRVQKRSGS
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-adenosyl-L-methionine; protein L-glutamate

Specific reaction: S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]