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The map label for this gene is hbpA [H]

Identifier: 13488274

GI number: 13488274

Start: 243822

End: 245384

Strand: Direct

Name: hbpA [H]

Synonym: mlr9267

Alternate gene names: 13488274

Gene position: 243822-245384 (Clockwise)

Preceding gene: 13488273

Following gene: 13488360

Centisome position: 69.29

GC content: 60.4

Gene sequence:

>1563_bases
ATGCAAGCATCACGTCGCACATTTCTTGGCATGACGGCCTTGGCCGCCGGCGCAATAGCATTGCCGCGGTCCATCGTGGC
CGCAGCCGGCCAGCGGCCGACCCTCAATATTGCCGTTCAGGACTTGCGTACCATTCTGGAACCGGTAGATAGCTCCTCTA
ACGCCAATGTTTCCTGGCGCGTGCAGTACAGTATCTATGATCAAATCCTGCGAACCGATTACAATGATAGCTATCGCCTG
CACTTCGCGCTCGCGAAGTCGCTGAAGCAGATCGACGCGAAGACCTATGAGATTGGCCTGCGCGACAATGTGAAGTTCCA
TGACGGTTCCACAATGACGGCCGCCGACGTGGTGTTCTCGCTTGGCGTGGAGCGGTTGCGCGGCGAGAAGGCGCCTGGCG
CGGCAGTGTTTGGAAACTTTCTTGCCTCGCTTGCCGATGTGCAGAGGATCGATGATCGAACCGTCCGTCTGGTTACCAAG
ACGGGGGATCCGGTCTTCACCGACCGTCTCGGCGCCTGGGGCGCCCAGATCATCAGCGCCAAGGCATGGAAGGCCGCCAC
GAATTACAACGCGTGGGCAGCGCGGCCGATCGGCACGGGTCCTTACAAGGTCGGCGAAGTGCGAACGGGCGATGAGATAA
CGCTGATCGCGCACGACGACTATTGGGGTGGCCAGCCGCCGTTCGCCTCCATCCGCTTCAAGCGCTTGCCCGAGATTGCC
GCGCGCATCGCCGGCCTCAAAGCTGGTGACTACGATATCGTCACCGATATTACCCCGGATCACATTGCGGAGATTGACGG
CGTCAACGGCCTTTCAGTTATTGGTGGCGAAACCATGTGGTTCCGCTTTCTCACATTTGCTCCGCTCAGGACTCCCGTCC
TGAAGGATACCAATCTGCGACGTGCGCTTGCGTCCGCCATCGATCGGGATGCGCTGGCAGACGGCCTCTGGAACGGCCGC
GCGAAGGCGCTCAACGGCTGGCAGCTGCCGATCTTCGGCGAAATCAATGACCCCGAGCGCAAGGGCGTGTCCTACGACCC
GGTTCTCGTCAAGGAGCTGCTGGCCAAGTCGTCCTACAGGGGCGAGGAGATTCCCTATCCGACCGTCGGGACCTACTACC
CCATGCAGCTTGCGGACAACCAGGCTCTGGTGGAGATGTGGAAAGCCGCGGGCATCAATGTCAAGTTGATCATGTGCGAG
AACTGGGACCAGGTTGGCAATATTGGTGGCATCAATGATATGTCCGGTGGCGGCCTCTACGCCGATCCCGTCTCCATGCT
CTGGCAGATCATCGGGCCCACCAACGACCTGCAGGCCTCGGGCGGCTGGACCAATGCCGAATTCAATGCGCTCGGCAATC
AGCTTGAGACGACGCTGGACCCCGCCAAGCGCAAAGCCGCGTTCAAGCGCATGCAGGACATTGTCGAGATCGAAGATCCC
TGCGTCACGCCGCTGCACTCCATGCCGTTCATCTATGGGCAAAGGAAGGACATCTCTTGGCAGCCCAATCCGTTGCCGCA
GATGTGGCTGGGCCCGAAGTTGCCGCCCGAGCCTGTGGCGTGA

Upstream 100 bases:

>100_bases
ACGATCCGTCGTACTCCCGCGAAAAACTGTCATATGCTTGCTCTATATCTCGGTTGGGACGGGACTGTCTGTTCGTCCGC
CAGAGAGAAGGGTTTCTGAC

Downstream 100 bases:

>100_bases
GCCCGATGCTGAACGCATTCACCACGAACAGCGCGGATGTCGCGCTGTTCGACAGGATCGATTTTGCCGCCTTGCGTATG
CTGCGCGAGCAGCGGATCGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Hemin-binding lipoprotein [H]

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MQASRRTFLGMTALAAGAIALPRSIVAAAGQRPTLNIAVQDLRTILEPVDSSSNANVSWRVQYSIYDQILRTDYNDSYRL
HFALAKSLKQIDAKTYEIGLRDNVKFHDGSTMTAADVVFSLGVERLRGEKAPGAAVFGNFLASLADVQRIDDRTVRLVTK
TGDPVFTDRLGAWGAQIISAKAWKAATNYNAWAARPIGTGPYKVGEVRTGDEITLIAHDDYWGGQPPFASIRFKRLPEIA
ARIAGLKAGDYDIVTDITPDHIAEIDGVNGLSVIGGETMWFRFLTFAPLRTPVLKDTNLRRALASAIDRDALADGLWNGR
AKALNGWQLPIFGEINDPERKGVSYDPVLVKELLAKSSYRGEEIPYPTVGTYYPMQLADNQALVEMWKAAGINVKLIMCE
NWDQVGNIGGINDMSGGGLYADPVSMLWQIIGPTNDLQASGGWTNAEFNALGNQLETTLDPAKRKAAFKRMQDIVEIEDP
CVTPLHSMPFIYGQRKDISWQPNPLPQMWLGPKLPPEPVA

Sequences:

>Translated_520_residues
MQASRRTFLGMTALAAGAIALPRSIVAAAGQRPTLNIAVQDLRTILEPVDSSSNANVSWRVQYSIYDQILRTDYNDSYRL
HFALAKSLKQIDAKTYEIGLRDNVKFHDGSTMTAADVVFSLGVERLRGEKAPGAAVFGNFLASLADVQRIDDRTVRLVTK
TGDPVFTDRLGAWGAQIISAKAWKAATNYNAWAARPIGTGPYKVGEVRTGDEITLIAHDDYWGGQPPFASIRFKRLPEIA
ARIAGLKAGDYDIVTDITPDHIAEIDGVNGLSVIGGETMWFRFLTFAPLRTPVLKDTNLRRALASAIDRDALADGLWNGR
AKALNGWQLPIFGEINDPERKGVSYDPVLVKELLAKSSYRGEEIPYPTVGTYYPMQLADNQALVEMWKAAGINVKLIMCE
NWDQVGNIGGINDMSGGGLYADPVSMLWQIIGPTNDLQASGGWTNAEFNALGNQLETTLDPAKRKAAFKRMQDIVEIEDP
CVTPLHSMPFIYGQRKDISWQPNPLPQMWLGPKLPPEPVA
>Mature_520_residues
MQASRRTFLGMTALAAGAIALPRSIVAAAGQRPTLNIAVQDLRTILEPVDSSSNANVSWRVQYSIYDQILRTDYNDSYRL
HFALAKSLKQIDAKTYEIGLRDNVKFHDGSTMTAADVVFSLGVERLRGEKAPGAAVFGNFLASLADVQRIDDRTVRLVTK
TGDPVFTDRLGAWGAQIISAKAWKAATNYNAWAARPIGTGPYKVGEVRTGDEITLIAHDDYWGGQPPFASIRFKRLPEIA
ARIAGLKAGDYDIVTDITPDHIAEIDGVNGLSVIGGETMWFRFLTFAPLRTPVLKDTNLRRALASAIDRDALADGLWNGR
AKALNGWQLPIFGEINDPERKGVSYDPVLVKELLAKSSYRGEEIPYPTVGTYYPMQLADNQALVEMWKAAGINVKLIMCE
NWDQVGNIGGINDMSGGGLYADPVSMLWQIIGPTNDLQASGGWTNAEFNALGNQLETTLDPAKRKAAFKRMQDIVEIEDP
CVTPLHSMPFIYGQRKDISWQPNPLPQMWLGPKLPPEPVA

Specific function: Important role in heme acquisition or metabolism [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=428, Percent_Identity=24.2990654205607, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787551, Length=374, Percent_Identity=26.2032085561497, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787052, Length=510, Percent_Identity=23.5294117647059, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787762, Length=464, Percent_Identity=24.1379310344828, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87082063, Length=282, Percent_Identity=23.4042553191489, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 57119; Mature: 57119

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASRRTFLGMTALAAGAIALPRSIVAAAGQRPTLNIAVQDLRTILEPVDSSSNANVSWR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEE
VQYSIYDQILRTDYNDSYRLHFALAKSLKQIDAKTYEIGLRDNVKFHDGSTMTAADVVFS
EEHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHHHHH
LGVERLRGEKAPGAAVFGNFLASLADVQRIDDRTVRLVTKTGDPVFTDRLGAWGAQIISA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KAWKAATNYNAWAARPIGTGPYKVGEVRTGDEITLIAHDDYWGGQPPFASIRFKRLPEIA
HHHHCCCCCCCEECCCCCCCCEEECEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ARIAGLKAGDYDIVTDITPDHIAEIDGVNGLSVIGGETMWFRFLTFAPLRTPVLKDTNLR
HHHHCCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHH
RALASAIDRDALADGLWNGRAKALNGWQLPIFGEINDPERKGVSYDPVLVKELLAKSSYR
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEECCCCCHHHCCCCCCHHHHHHHHHHCCCC
GEEIPYPTVGTYYPMQLADNQALVEMWKAAGINVKLIMCENWDQVGNIGGINDMSGGGLY
CCCCCCCCCCCEECCEECCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCEE
ADPVSMLWQIIGPTNDLQASGGWTNAEFNALGNQLETTLDPAKRKAAFKRMQDIVEIEDP
ECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
CVTPLHSMPFIYGQRKDISWQPNPLPQMWLGPKLPPEPVA
CCCCHHHCCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MQASRRTFLGMTALAAGAIALPRSIVAAAGQRPTLNIAVQDLRTILEPVDSSSNANVSWR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCCCCCCCEEEE
VQYSIYDQILRTDYNDSYRLHFALAKSLKQIDAKTYEIGLRDNVKFHDGSTMTAADVVFS
EEHHHHHHHHHCCCCCCEEEHHHHHHHHHHHCCCEEEECCCCCCEECCCCCCHHHHHHHH
LGVERLRGEKAPGAAVFGNFLASLADVQRIDDRTVRLVTKTGDPVFTDRLGAWGAQIISA
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
KAWKAATNYNAWAARPIGTGPYKVGEVRTGDEITLIAHDDYWGGQPPFASIRFKRLPEIA
HHHHCCCCCCCEECCCCCCCCEEECEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
ARIAGLKAGDYDIVTDITPDHIAEIDGVNGLSVIGGETMWFRFLTFAPLRTPVLKDTNLR
HHHHCCCCCCCEEEECCCHHHHHHHCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCHHH
RALASAIDRDALADGLWNGRAKALNGWQLPIFGEINDPERKGVSYDPVLVKELLAKSSYR
HHHHHHHHHHHHHHHHCCCCHHCCCCCCCEEECCCCCHHHCCCCCCHHHHHHHHHHCCCC
GEEIPYPTVGTYYPMQLADNQALVEMWKAAGINVKLIMCENWDQVGNIGGINDMSGGGLY
CCCCCCCCCCCEECCEECCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCEE
ADPVSMLWQIIGPTNDLQASGGWTNAEFNALGNQLETTLDPAKRKAAFKRMQDIVEIEDP
ECHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
CVTPLHSMPFIYGQRKDISWQPNPLPQMWLGPKLPPEPVA
CCCCHHHCCEEECCCCCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1339409; 7542800; 2041470 [H]