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The map label for this gene is virB8 [H]

Identifier: 13488266

GI number: 13488266

Start: 235069

End: 235800

Strand: Direct

Name: virB8 [H]

Synonym: mlr9256

Alternate gene names: 13488266

Gene position: 235069-235800 (Clockwise)

Preceding gene: 13488265

Following gene: 13488267

Centisome position: 66.8

GC content: 60.52

Gene sequence:

>732_bases
ATGAGCGATGTGAAGGAAAGCGCTGGGGGACCGGCGAAGGAAACCGAGTCTCCATTGCCGTACTACGTGGATGCGGCAAT
CTGGGAAAAAGATATCGCGCGGCGCAATCGCTGGTCGCGATCTCTGGCGTGGTGTGTGGCAAGCGTCGCCTCGGTCATTG
CCGTTGCAGCGGTGGGCGCGCTGATCCTTGCGCTGCCGCTCAAGACTTATGAGCCCTACATGGTGGTGGTCGACAAATCC
ACCGGGTTTGTCGAGGTCAAGCGGCCGCTGGCCGACGGTCCTTTGCAGCAGGACGAGTCGATGGGGATGTTCGATATCGT
CCGTTATGTGAAGGCTCGCGAGACCTACGATCCGAAGGCGCTGAAGGACAACTTCGATCTCGCGCAATTGCTGAGCACCG
GCGATGCCTCGAGGGAGCTCGTCGAGCTTTTCAGCCCGGCGAACAAGGTGACGAACCCGGTCGTGCTCTACGGGCGAAAC
ACCGTCGTGGCTGTGACCGTGAAGTCCGTGACATTTCCCAACCAGAGGACCGCCCTGGTCCGGTTCATGACTGAGGAAAA
GAGCCAGGCGAACACCGTGCAGCGGCATTGGGTGTCGTTGATCCGGTTCCGCTACACCGGCGTGCCAGCTAAAAACGAGA
TCCGCTTTCAAAATCCGCTCGGCTTCCAGGTGCTCGAATATCGGCGCGATCAGGAGACGGCGCCGGCACCCACCGCGGAG
ACACCGCAATGA

Upstream 100 bases:

>100_bases
CGGGCGCGCGTGAGCCTCGCACAGAGAAGGTACGAGGATTCAGCGGAATATGCACGGCTTTCCCGAAAGCTGACGGATCC
CGCATAGGTTTGGGGCACCA

Downstream 100 bases:

>100_bases
ACGTCGCCGGCATCCTGATGGCAATCGGGCTCGGCGTTTGCGCGGTTCAGCCGGCCCTTGCCGAACAGACTCCTCGGGCG
GGATCTCGTGACAGTCGCAT

Product: conjugal transfer protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSDVKESAGGPAKETESPLPYYVDAAIWEKDIARRNRWSRSLAWCVASVASVIAVAAVGALILALPLKTYEPYMVVVDKS
TGFVEVKRPLADGPLQQDESMGMFDIVRYVKARETYDPKALKDNFDLAQLLSTGDASRELVELFSPANKVTNPVVLYGRN
TVVAVTVKSVTFPNQRTALVRFMTEEKSQANTVQRHWVSLIRFRYTGVPAKNEIRFQNPLGFQVLEYRRDQETAPAPTAE
TPQ

Sequences:

>Translated_243_residues
MSDVKESAGGPAKETESPLPYYVDAAIWEKDIARRNRWSRSLAWCVASVASVIAVAAVGALILALPLKTYEPYMVVVDKS
TGFVEVKRPLADGPLQQDESMGMFDIVRYVKARETYDPKALKDNFDLAQLLSTGDASRELVELFSPANKVTNPVVLYGRN
TVVAVTVKSVTFPNQRTALVRFMTEEKSQANTVQRHWVSLIRFRYTGVPAKNEIRFQNPLGFQVLEYRRDQETAPAPTAE
TPQ
>Mature_242_residues
SDVKESAGGPAKETESPLPYYVDAAIWEKDIARRNRWSRSLAWCVASVASVIAVAAVGALILALPLKTYEPYMVVVDKST
GFVEVKRPLADGPLQQDESMGMFDIVRYVKARETYDPKALKDNFDLAQLLSTGDASRELVELFSPANKVTNPVVLYGRNT
VVAVTVKSVTFPNQRTALVRFMTEEKSQANTVQRHWVSLIRFRYTGVPAKNEIRFQNPLGFQVLEYRRDQETAPAPTAET
PQ

Specific function: Component of the type IV secretion system virB/virD4 which could be a major virulence determinant for subversion of human endothelial cell (HEC) function [H]

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007430 [H]

Pfam domain/function: PF04335 VirB8 [H]

EC number: NA

Molecular weight: Translated: 27143; Mature: 27012

Theoretical pI: Translated: 8.87; Mature: 8.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVKESAGGPAKETESPLPYYVDAAIWEKDIARRNRWSRSLAWCVASVASVIAVAAVGA
CCCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILALPLKTYEPYMVVVDKSTGFVEVKRPLADGPLQQDESMGMFDIVRYVKARETYDPKA
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
LKDNFDLAQLLSTGDASRELVELFSPANKVTNPVVLYGRNTVVAVTVKSVTFPNQRTALV
HHCCCHHHHHHHCCCHHHHHHHHHCCHHHCCCCEEEEECCEEEEEEEEEEECCCCHHHHH
RFMTEEKSQANTVQRHWVSLIRFRYTGVPAKNEIRFQNPLGFQVLEYRRDQETAPAPTAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCC
TPQ
CCC
>Mature Secondary Structure 
SDVKESAGGPAKETESPLPYYVDAAIWEKDIARRNRWSRSLAWCVASVASVIAVAAVGA
CCCCHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILALPLKTYEPYMVVVDKSTGFVEVKRPLADGPLQQDESMGMFDIVRYVKARETYDPKA
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHH
LKDNFDLAQLLSTGDASRELVELFSPANKVTNPVVLYGRNTVVAVTVKSVTFPNQRTALV
HHCCCHHHHHHHCCCHHHHHHHHHCCHHHCCCCEEEEECCEEEEEEEEEEECCCCHHHHH
RFMTEEKSQANTVQRHWVSLIRFRYTGVPAKNEIRFQNPLGFQVLEYRRDQETAPAPTAE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCC
TPQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA