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The map label for this gene is virB4 [H]

Identifier: 13488262

GI number: 13488262

Start: 229743

End: 232154

Strand: Direct

Name: virB4 [H]

Synonym: mlr9251

Alternate gene names: 13488262

Gene position: 229743-232154 (Clockwise)

Preceding gene: 13488261

Following gene: 13488263

Centisome position: 65.28

GC content: 59.74

Gene sequence:

>2412_bases
GTGGCAATCGCGCGGGCTCTTCGTGATGAACTGAGTTTCGGGGCCGTATCTCGTCGGGAAAACCCCGTTTCGGCTCACAT
ACCCTATTCACGGCATGTGTCCGACGAGGTGATCAAGACGCAGGACGGCATGTTGATGTCCTTCGTTCACATGAGCGGCC
TGTGCTTCGAGACGATCGATATCGCGGAGATCAATTCGCGCCTGCTCGGCCGCAACGACCTTATTCGGGGATTGGCGAAT
TCGCGCTATGCGATCTACTCGCACATCGTGCGCCGCGAGGTGAAGCCGGCGATCGAGTCGAGCTTCGAAAATAAGTTCTG
CCAAGAGCTGGATCAGCGCTATACGGCGGCCCTGCGCACCCAGCGCATGTTTGTGAACGACATCTACCTGACGATCGTGC
GGCGCGAGCTGCAGGGCACGGTCGGAACGGCGGATCTTGTGATCCGCAAGCTTTTGGGACGCAGGGCTGCGGACGGTCGC
TCTTCGAGCGAGGAGCGGGCGCTGATCGAACTCACGGACGTGATGAAAGCCGTCCGAGAGAGCCTGCAGGCGTATGGCGC
GCGGGTTCTCACCGTCGTCAAGCGCAATGACGTTTGGCATTCCGAGCCGCTGGAATTCCTGGTGCAGCTTGTCAATGGCG
GGTTTCCGCGCCCGATGGCACTGCCGCGCATGAAGCTCGCGGAGGCGCTGGCGACGAAGCGGCTGTTCTTTGGCCCGAAT
GCGCTGGAAATTCGCGGGGCATTCCCTGGCGAAACGCGATACGGCGCCATCATTTCGGTAGGCGAGTACCCCTCCATTAC
GGGACCGGGGATGCTCAACCCAATGCTGAAGGTGCCGCACGAATTCATCGTGACGCAATCCTTCGCCATCATCGACAAGC
CCCAGGCGCTCACCCAGATGGAGCGGGTTGGGCGCAAGATCGACATGTCGGATGAAGCCGACTCGATCGTGGGTGATCAG
CTCGGTGAAGCGCGCGATGAGCTTCTGTCCTCGGAAGCGCTCTACGGCTTGCATCACCTGACGGTGCTGTGCCTCGGGAA
AACCATGGACGACGTGGCACGCTGCGTGACCGATGTCGGCACGGCGCTGACGGAGGTGTCCGCCCTGTGGGTTCGCGAGG
ACCTGAATTGTGAACCTGCGTTCTGGGCCACCCTGCCTGGCAATTTCGCCTATGTGGCGCGCAAGTCCATGATCTCATCT
AAGAATATGGCGGGCTTCTCGGCCTTCCATAATTATCCCAGCGGCCGGACTGGCGGGGTTCATTGGGGAATGCCGATAAG
CGTCCTCGAGACGACGTCGCAAACGGCCTACTTCTTCAACTTCCACGTTCGAGACCTCGGCAACTTCACCCTGAACGGTC
CCTCGGGCTCGGGCAAGACGGTGCTGCTCGGCTTTCTTGCAGCACAGGCGCAACGCATCACGCCGCGCCCCAAGCTGGTG
ATGATCGACAAGGATAGGGGCTTGGATATCTTCGTGCGGTCGCTTGGCGGCCGCTATGAGGTTCTGAAGGCCGGTGAGCC
TTCCGGCTTCAATCCGTTGCGCCTGCCCGATACGCCGCGCAACCGGGAATTCCTCTTCCAGCTTTTCAGCTTCATCCTGC
GTCGATCCGACGGATATGCCCACTCGACGCGCGAGGAGCAAGTCATTCGCAACGCGATCGCCCAGGTGGCGAGCGCGCCG
CCCGAAGGTCGAACCATGGAGGAGTTCGCGGAGCTGTTGCGCGGTGCGATGCGCGCCGGCGATGACGACCTGTATTCGCG
CCTTCAGCCATGGATGCGGCCGGATCAGCGCGGTTGGTTGTTCAACAACGCGGAAGACTCGTTTTCGATGTCGTCCATTT
TCGGCTTCGACATGACGAGCGTGTTGAGCGACGCAACGACCAGTACGGCGGCCTTGCTCTATATTTTTCATCGTATCGAG
GAGCTGCTTGACGGGCAACCGGTCATGATCTTCCTCGATGAAGGCTGGAAGCTCCTGGACAATGATATTTTCAGCTACTT
CATCAAGGACAAGCTGAAAACCATTCGTAAGTTGAATGGCATCATTGGCTTCGGCACGCAGTCCGCGGCGGATATTGTGA
AGTCATCGATCGCCAACACGCTGATCGAACAGACCCCGACCAATATCTTTTTCCCCAACCCGAAGGCCGACGAGGAATCG
CACAGGGCGTTCGGACTGTCGGAGCGTGAGATTAAATGGATCCGCGAAACCCCGCCGGAAGCCCGCAAATTCCTGATCAA
GCATGATCAGGACAGCGTCATTGCGCGCCTCAATCTGGGCGCGATGCCTGACGCGATCAAGATCCTCTCCGGTCGAATTG
AGACAGTTCAGGAGCTCGACGCGCTGCGCGCGCGCGTGGGCGACGATCCGGACGTTTGGTTGCCAGTCTTTCTTGGGAGG
GACCCGGAATGA

Upstream 100 bases:

>100_bases
TGCGCGATTCCATGTTCATCGAGATCATGTTCATCCGTTCACGAAAGACGCCACCCCGAAGCGCCAGCTTCTGGGGTGCG
CGCAGTTATCGGGTGTGACC

Downstream 100 bases:

>100_bases
AGACCATCATGGGCGCGACGGTCGCCCTTGTGACGATTTTCGCGGGATCGACGGCCATAGCGGCCGTGCCGATTTCTGAC
GGCCCGATCTTGTCACAGCG

Product: conjugal transfer protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MAIARALRDELSFGAVSRRENPVSAHIPYSRHVSDEVIKTQDGMLMSFVHMSGLCFETIDIAEINSRLLGRNDLIRGLAN
SRYAIYSHIVRREVKPAIESSFENKFCQELDQRYTAALRTQRMFVNDIYLTIVRRELQGTVGTADLVIRKLLGRRAADGR
SSSEERALIELTDVMKAVRESLQAYGARVLTVVKRNDVWHSEPLEFLVQLVNGGFPRPMALPRMKLAEALATKRLFFGPN
ALEIRGAFPGETRYGAIISVGEYPSITGPGMLNPMLKVPHEFIVTQSFAIIDKPQALTQMERVGRKIDMSDEADSIVGDQ
LGEARDELLSSEALYGLHHLTVLCLGKTMDDVARCVTDVGTALTEVSALWVREDLNCEPAFWATLPGNFAYVARKSMISS
KNMAGFSAFHNYPSGRTGGVHWGMPISVLETTSQTAYFFNFHVRDLGNFTLNGPSGSGKTVLLGFLAAQAQRITPRPKLV
MIDKDRGLDIFVRSLGGRYEVLKAGEPSGFNPLRLPDTPRNREFLFQLFSFILRRSDGYAHSTREEQVIRNAIAQVASAP
PEGRTMEEFAELLRGAMRAGDDDLYSRLQPWMRPDQRGWLFNNAEDSFSMSSIFGFDMTSVLSDATTSTAALLYIFHRIE
ELLDGQPVMIFLDEGWKLLDNDIFSYFIKDKLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEES
HRAFGLSEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELDALRARVGDDPDVWLPVFLGR
DPE

Sequences:

>Translated_803_residues
MAIARALRDELSFGAVSRRENPVSAHIPYSRHVSDEVIKTQDGMLMSFVHMSGLCFETIDIAEINSRLLGRNDLIRGLAN
SRYAIYSHIVRREVKPAIESSFENKFCQELDQRYTAALRTQRMFVNDIYLTIVRRELQGTVGTADLVIRKLLGRRAADGR
SSSEERALIELTDVMKAVRESLQAYGARVLTVVKRNDVWHSEPLEFLVQLVNGGFPRPMALPRMKLAEALATKRLFFGPN
ALEIRGAFPGETRYGAIISVGEYPSITGPGMLNPMLKVPHEFIVTQSFAIIDKPQALTQMERVGRKIDMSDEADSIVGDQ
LGEARDELLSSEALYGLHHLTVLCLGKTMDDVARCVTDVGTALTEVSALWVREDLNCEPAFWATLPGNFAYVARKSMISS
KNMAGFSAFHNYPSGRTGGVHWGMPISVLETTSQTAYFFNFHVRDLGNFTLNGPSGSGKTVLLGFLAAQAQRITPRPKLV
MIDKDRGLDIFVRSLGGRYEVLKAGEPSGFNPLRLPDTPRNREFLFQLFSFILRRSDGYAHSTREEQVIRNAIAQVASAP
PEGRTMEEFAELLRGAMRAGDDDLYSRLQPWMRPDQRGWLFNNAEDSFSMSSIFGFDMTSVLSDATTSTAALLYIFHRIE
ELLDGQPVMIFLDEGWKLLDNDIFSYFIKDKLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEES
HRAFGLSEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELDALRARVGDDPDVWLPVFLGR
DPE
>Mature_802_residues
AIARALRDELSFGAVSRRENPVSAHIPYSRHVSDEVIKTQDGMLMSFVHMSGLCFETIDIAEINSRLLGRNDLIRGLANS
RYAIYSHIVRREVKPAIESSFENKFCQELDQRYTAALRTQRMFVNDIYLTIVRRELQGTVGTADLVIRKLLGRRAADGRS
SSEERALIELTDVMKAVRESLQAYGARVLTVVKRNDVWHSEPLEFLVQLVNGGFPRPMALPRMKLAEALATKRLFFGPNA
LEIRGAFPGETRYGAIISVGEYPSITGPGMLNPMLKVPHEFIVTQSFAIIDKPQALTQMERVGRKIDMSDEADSIVGDQL
GEARDELLSSEALYGLHHLTVLCLGKTMDDVARCVTDVGTALTEVSALWVREDLNCEPAFWATLPGNFAYVARKSMISSK
NMAGFSAFHNYPSGRTGGVHWGMPISVLETTSQTAYFFNFHVRDLGNFTLNGPSGSGKTVLLGFLAAQAQRITPRPKLVM
IDKDRGLDIFVRSLGGRYEVLKAGEPSGFNPLRLPDTPRNREFLFQLFSFILRRSDGYAHSTREEQVIRNAIAQVASAPP
EGRTMEEFAELLRGAMRAGDDDLYSRLQPWMRPDQRGWLFNNAEDSFSMSSIFGFDMTSVLSDATTSTAALLYIFHRIEE
LLDGQPVMIFLDEGWKLLDNDIFSYFIKDKLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEESH
RAFGLSEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELDALRARVGDDPDVWLPVFLGRD
PE

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 89929; Mature: 89798

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIARALRDELSFGAVSRRENPVSAHIPYSRHVSDEVIKTQDGMLMSFVHMSGLCFETID
CHHHHHHHHHHHHCCHHCCCCCCEEECCCHHCCCHHHHHCCCCHHHHHHHHCCCHHHHHH
IAEINSRLLGRNDLIRGLANSRYAIYSHIVRREVKPAIESSFENKFCQELDQRYTAALRT
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMFVNDIYLTIVRRELQGTVGTADLVIRKLLGRRAADGRSSSEERALIELTDVMKAVRE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SLQAYGARVLTVVKRNDVWHSEPLEFLVQLVNGGFPRPMALPRMKLAEALATKRLFFGPN
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCC
ALEIRGAFPGETRYGAIISVGEYPSITGPGMLNPMLKVPHEFIVTQSFAIIDKPQALTQM
CEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHCCHHEECCCHHHHHH
ERVGRKIDMSDEADSIVGDQLGEARDELLSSEALYGLHHLTVLCLGKTMDDVARCVTDVG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TALTEVSALWVREDLNCEPAFWATLPGNFAYVARKSMISSKNMAGFSAFHNYPSGRTGGV
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCE
HWGMPISVLETTSQTAYFFNFHVRDLGNFTLNGPSGSGKTVLLGFLAAQAQRITPRPKLV
ECCCCHHHHHCCCCEEEEEEEEEEECCCEEEECCCCCCCEEHHHHHHHHHHHCCCCCCEE
MIDKDRGLDIFVRSLGGRYEVLKAGEPSGFNPLRLPDTPRNREFLFQLFSFILRRSDGYA
EEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
HSTREEQVIRNAIAQVASAPPEGRTMEEFAELLRGAMRAGDDDLYSRLQPWMRPDQRGWL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
FNNAEDSFSMSSIFGFDMTSVLSDATTSTAALLYIFHRIEELLDGQPVMIFLDEGWKLLD
CCCCCCCCCHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
NDIFSYFIKDKLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEES
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCH
HRAFGLSEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELD
HHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ALRARVGDDPDVWLPVFLGRDPE
HHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
AIARALRDELSFGAVSRRENPVSAHIPYSRHVSDEVIKTQDGMLMSFVHMSGLCFETID
HHHHHHHHHHHHCCHHCCCCCCEEECCCHHCCCHHHHHCCCCHHHHHHHHCCCHHHHHH
IAEINSRLLGRNDLIRGLANSRYAIYSHIVRREVKPAIESSFENKFCQELDQRYTAALRT
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRMFVNDIYLTIVRRELQGTVGTADLVIRKLLGRRAADGRSSSEERALIELTDVMKAVRE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SLQAYGARVLTVVKRNDVWHSEPLEFLVQLVNGGFPRPMALPRMKLAEALATKRLFFGPN
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCC
ALEIRGAFPGETRYGAIISVGEYPSITGPGMLNPMLKVPHEFIVTQSFAIIDKPQALTQM
CEEEECCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHCCHHHHHHCCHHEECCCHHHHHH
ERVGRKIDMSDEADSIVGDQLGEARDELLSSEALYGLHHLTVLCLGKTMDDVARCVTDVG
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
TALTEVSALWVREDLNCEPAFWATLPGNFAYVARKSMISSKNMAGFSAFHNYPSGRTGGV
HHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCE
HWGMPISVLETTSQTAYFFNFHVRDLGNFTLNGPSGSGKTVLLGFLAAQAQRITPRPKLV
ECCCCHHHHHCCCCEEEEEEEEEEECCCEEEECCCCCCCEEHHHHHHHHHHHCCCCCCEE
MIDKDRGLDIFVRSLGGRYEVLKAGEPSGFNPLRLPDTPRNREFLFQLFSFILRRSDGYA
EEECCCCHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
HSTREEQVIRNAIAQVASAPPEGRTMEEFAELLRGAMRAGDDDLYSRLQPWMRPDQRGWL
CCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
FNNAEDSFSMSSIFGFDMTSVLSDATTSTAALLYIFHRIEELLDGQPVMIFLDEGWKLLD
CCCCCCCCCHHHHHCCCHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
NDIFSYFIKDKLKTIRKLNGIIGFGTQSAADIVKSSIANTLIEQTPTNIFFPNPKADEES
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCH
HRAFGLSEREIKWIRETPPEARKFLIKHDQDSVIARLNLGAMPDAIKILSGRIETVQELD
HHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHH
ALRARVGDDPDVWLPVFLGRDPE
HHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]