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The map label for this gene is cysK2 [H]

Identifier: 13488127

GI number: 13488127

Start: 210477

End: 211532

Strand: Reverse

Name: cysK2 [H]

Synonym: mll9227

Alternate gene names: 13488127

Gene position: 211532-210477 (Counterclockwise)

Preceding gene: 13488128

Following gene: 161621457

Centisome position: 60.11

GC content: 57.29

Gene sequence:

>1056_bases
ATGGACGCTGTTCGCAAACGCCTGCCATACGCCGGATATTCCGAAGCCTATGCCCTCCCCAGCATTATTAAGTGTGAGAC
AAATCTTTATCTCGCGCAGTTTGCGTTCATGAAGCTTCTCCCAGCGAAGTACATCATTGAGCAAGCGCTGGCTCAAGGGA
CTTTGACGCACGGCATGAAAGTTCTCGAGACCTCGAGCGGAACCTTCGCGCTGGGTCTGGCCGTAGTTTGCCGGGAACGT
GGATTCCAGCTCGAAATCTTCACCGACCCAGTGATGGACAGAGGCCTGGAAAACAGGTTGACCTCCTTGGGAGCCGAGGT
CTGCATCATAACTGAAAAGGCGAGGGAGGGTGGATATCAAAGAAGCCGCCTCGATGCATTGCATGCCAGGATGAGACAGT
TAGGCCACTCCTGTTTCTGGCCCCGGCAATACGAAACACCGGACAACCCCGCAGCCTATAAGCTCTTCGCCGATCAAATA
TCGGGGATGCTGGACGCCGACGTAACGTTGGTCGGTTCTGTGGGATCAGGCGGCTCTACCTGCGGAACGATAGAGCGCCT
CCGCCAAAAAGCCCCTGGCGCGCGCCTAATTGGAGTGGATACCTTCAACAGCGTCATTTTTGGCCAACAGGATGGCGAAC
GGAAGCTGCGGGGGCTGGGGAACAGTCTTGTCCCCAAGAACGTGAAGCATGAACTCTACGACGAGGTGCATCTCATCTCG
GCTCCGCTCGCATTTGCTGCAACGCGAACCCTCCACGAGCGGCACGCCGTTTTCGCCGGTCCGACCTCTGGAGCGAGCTA
CATTGTCGGGCGTTGGCGCGCTCGGCAGTATCCAGGAGAAACCGTCGTCGTCATATGTCCCGATGAGGGGCACCGCTACG
TAGAAACCGCTTACGACCACGAGTGGCTCAGGCAGAACGGATGCCTACATGAGGGAGCTCTCCAGGACACTCCGTCTAGC
GAAGAGCATCCCTCGACCGCTCTCCCGCCATGGAACCGATACCTTTGGAGGCGGAAAAGCCGGGAAGCTGTCCTGAGCCT
TTTGGAGGAAGTTTGA

Upstream 100 bases:

>100_bases
TCCACGGAAGCTTCGGTCCGTGCTCGCCCGTGTAGCGCCGTTCTACAACTCGCCAAAAATATATCTGACCATGTGACTGG
CTGCAGGTGAGGCAAGCAAT

Downstream 100 bases:

>100_bases
TGAACAGAGGTAGCTTCCTTTTCGTGGAAAGCAACACGACTGGCACTGGCGAACTATTGATGAAGCGGGCCCGACTCCTG
GGCTTTGAAACCTACCTCGT

Product: cysteine synthase

Products: @CYST01txt*L-cysteine!; acetate; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MDAVRKRLPYAGYSEAYALPSIIKCETNLYLAQFAFMKLLPAKYIIEQALAQGTLTHGMKVLETSSGTFALGLAVVCRER
GFQLEIFTDPVMDRGLENRLTSLGAEVCIITEKAREGGYQRSRLDALHARMRQLGHSCFWPRQYETPDNPAAYKLFADQI
SGMLDADVTLVGSVGSGGSTCGTIERLRQKAPGARLIGVDTFNSVIFGQQDGERKLRGLGNSLVPKNVKHELYDEVHLIS
APLAFAATRTLHERHAVFAGPTSGASYIVGRWRARQYPGETVVVICPDEGHRYVETAYDHEWLRQNGCLHEGALQDTPSS
EEHPSTALPPWNRYLWRRKSREAVLSLLEEV

Sequences:

>Translated_351_residues
MDAVRKRLPYAGYSEAYALPSIIKCETNLYLAQFAFMKLLPAKYIIEQALAQGTLTHGMKVLETSSGTFALGLAVVCRER
GFQLEIFTDPVMDRGLENRLTSLGAEVCIITEKAREGGYQRSRLDALHARMRQLGHSCFWPRQYETPDNPAAYKLFADQI
SGMLDADVTLVGSVGSGGSTCGTIERLRQKAPGARLIGVDTFNSVIFGQQDGERKLRGLGNSLVPKNVKHELYDEVHLIS
APLAFAATRTLHERHAVFAGPTSGASYIVGRWRARQYPGETVVVICPDEGHRYVETAYDHEWLRQNGCLHEGALQDTPSS
EEHPSTALPPWNRYLWRRKSREAVLSLLEEV
>Mature_351_residues
MDAVRKRLPYAGYSEAYALPSIIKCETNLYLAQFAFMKLLPAKYIIEQALAQGTLTHGMKVLETSSGTFALGLAVVCRER
GFQLEIFTDPVMDRGLENRLTSLGAEVCIITEKAREGGYQRSRLDALHARMRQLGHSCFWPRQYETPDNPAAYKLFADQI
SGMLDADVTLVGSVGSGGSTCGTIERLRQKAPGARLIGVDTFNSVIFGQQDGERKLRGLGNSLVPKNVKHELYDEVHLIS
APLAFAATRTLHERHAVFAGPTSGASYIVGRWRARQYPGETVVVICPDEGHRYVETAYDHEWLRQNGCLHEGALQDTPSS
EEHPSTALPPWNRYLWRRKSREAVLSLLEEV

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=281, Percent_Identity=27.7580071174377, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI295821200, Length=281, Percent_Identity=27.7580071174377, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI4557415, Length=281, Percent_Identity=27.7580071174377, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1788754, Length=268, Percent_Identity=25.3731343283582, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI2367138, Length=259, Percent_Identity=25.8687258687259, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17535051, Length=272, Percent_Identity=29.7794117647059, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17562970, Length=282, Percent_Identity=26.9503546099291, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI32566674, Length=243, Percent_Identity=28.3950617283951, Blast_Score=81, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17561720, Length=273, Percent_Identity=26.7399267399267, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25147552, Length=301, Percent_Identity=24.9169435215947, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32566672, Length=168, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=303, Percent_Identity=23.7623762376238, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24643623, Length=269, Percent_Identity=24.5353159851301, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129101, Length=269, Percent_Identity=24.5353159851301, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 2.5.1.47 [C]

Molecular weight: Translated: 39081; Mature: 39081

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAVRKRLPYAGYSEAYALPSIIKCETNLYLAQFAFMKLLPAKYIIEQALAQGTLTHGMK
CCHHHHHCCCCCCHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLETSSGTFALGLAVVCRERGFQLEIFTDPVMDRGLENRLTSLGAEVCIITEKAREGGYQ
EEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHCCCCEEEEEEHHHCCCCH
RSRLDALHARMRQLGHSCFWPRQYETPDNPAAYKLFADQISGMLDADVTLVGSVGSGGST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
CGTIERLRQKAPGARLIGVDTFNSVIFGQQDGERKLRGLGNSLVPKNVKHELYDEVHLIS
HHHHHHHHHHCCCCEEEEECCCCHHEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
APLAFAATRTLHERHAVFAGPTSGASYIVGRWRARQYPGETVVVICPDEGHRYVETAYDH
HHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
EWLRQNGCLHEGALQDTPSSEEHPSTALPPWNRYLWRRKSREAVLSLLEEV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDAVRKRLPYAGYSEAYALPSIIKCETNLYLAQFAFMKLLPAKYIIEQALAQGTLTHGMK
CCHHHHHCCCCCCHHHHCCCHHEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEE
VLETSSGTFALGLAVVCRERGFQLEIFTDPVMDRGLENRLTSLGAEVCIITEKAREGGYQ
EEECCCCCHHHHHHHHHHHCCCEEEEECCHHHHCCHHHHHHHCCCCEEEEEEHHHCCCCH
RSRLDALHARMRQLGHSCFWPRQYETPDNPAAYKLFADQISGMLDADVTLVGSVGSGGST
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCC
CGTIERLRQKAPGARLIGVDTFNSVIFGQQDGERKLRGLGNSLVPKNVKHELYDEVHLIS
HHHHHHHHHHCCCCEEEEECCCCHHEECCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
APLAFAATRTLHERHAVFAGPTSGASYIVGRWRARQYPGETVVVICPDEGHRYVETAYDH
HHHHHHHHHHHHHHHCEEECCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHH
EWLRQNGCLHEGALQDTPSSEEHPSTALPPWNRYLWRRKSREAVLSLLEEV
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 44000 [C]

Specific activity: 1100

Km value (mM): 0.12 {acetyl-CoA}} 3.6 {S2-}} 0.1 {S2-}} 20 {O-acetyl-L-Ser}} 7.1 {O-acetyl-L-Ser}} 5 {O-acetyl-L-Ser}} 0.7 {L-Ser}} [C]

Substrates: O-acetyl-L-serine; L-serine; acetyl-CoA; H2S [C]

Specific reaction: O-acetyl-L-serine + H2S = @CYST01.txt*L-cysteine! + acetate L-serine + acetyl-CoA + H2S = L-cysteine + Coenzyme A + acetate [C]

General reaction: Elimination; C-O bond cleavage [C]

Inhibitor: acetyl-CoA; D-cycloserine; L-cysteine; NH2OH; semicarbazide [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12368430 [H]