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The map label for this gene is thcD [H]

Identifier: 13488123

GI number: 13488123

Start: 199877

End: 201136

Strand: Reverse

Name: thcD [H]

Synonym: mll9218

Alternate gene names: 13488123

Gene position: 201136-199877 (Counterclockwise)

Preceding gene: 13488124

Following gene: 13488122

Centisome position: 57.16

GC content: 54.6

Gene sequence:

>1260_bases
ATGGGCGACTTTGTTATTATCGGAAGTGGAGTGGCTGGCCACCGTGCTGCTGCTGAACTTCACCGGCTCGACCCAAGTCA
GTCTGTGTACGTTGTCGGGACAGAACCAGGGGCGCCATATGACAGGCCACCCCTCTCAAAAGAATACCTGAAGACAACAG
AGCCAGTTCGTCCGGACCTTGGCCCTGCCGAGATCTATAAGTCGAACGTGACACTGCGGGACGGCCTCACTGCGACCAGC
ATCGATCGCCCCAATCAAACGATAGCGCTATCGGATGGCTCTTCTATTCAATATGATAGGCTCTTGTTGGCCACAGGATC
GCGGCTACGCCATCTGGATCTACCGAATATCGACCCTGCGCGCGTCTTCTATCTACGCACGATTTTAGATGCCCAGCGAC
TAAGGACGGCCCTGGATAACCATCTGCGTATTGCTATCATTGGAGGCGGCTTCGTTGGTTTGGAAATAGCAGCGGCAGCA
AAGCGCAATGGATGCACCGTCACCTTGCTGGAACGCGCACCGGAACTCTTGTCGCGTGGCGCCACGCCTCCACTCGCAGA
GTTTGCCCGCAAGATGCATTCCCGACAAGACGTCAGGATTTTTCTTGGCATCAACATCAGCGAAGCCTTCCAGGACGGAA
GCGATATTGTTCTGAGGTGGCCTGAAGGCGAGGTCAGATCTGACATTCTGGTCGTGGGTGTTGGCATTATTCCTAACATC
GAGCTTGCCCTTCAATGCGGACTAGCTACGGCTGATGGAATCCTGGTCGATCAACAATGCCGTACCTCCGACGAATGGAT
TTTTGCTGCTGGCGAGGTGACGAGCTATCCTGTCGATCGCCTAGGGGTGCATATACGCACAGAGTCATGGAGCTCTGCTT
CAGCTCAAGGCACCGTGGCAGCGACCAGCATGTTGGGAAGAAATGGTATGTTCAACGAGCTTCCATGGTTCTGGTCTGAC
CAATATGACTGCAACATTCAATGCCTTGGATTACCGAAGAGGGCATCGCGTTTCCACCAGATCGGCGACCTGAACTCCGA
GAGTTGGCTGCGCATTGGAGTTGACGATGCCGGATCCTTGGTAGGGGCTGAAAGCGTTAATAAAGGGCGGGAGATGTCGG
CACTTCGGCGCGCCGATCGAAGGGGCGAGCCAATTCCAACGGCGCTCATCAAGGACTCAATACTGGTACAGAGTTGCTCA
ACGGTTGGTGATTTGGGAGCTGGATCCGCGAGCCTCAATGTAAATGTGAGGACGCAATGA

Upstream 100 bases:

>100_bases
TGATGATCAAAATCGAGCGCAGCTGTATCTAGGAACGTTCCAGCCGTTGATGTTAAGCGTTATCAGATACCGCTTAGAGA
CGGACACGGGAACGGATCTG

Downstream 100 bases:

>100_bases
CCATGAATTGGGTAAGCCTGTGCCATCTGGATGACTTGGACACCGACGGGATGTATCCATTCGTCATTGGTGAAAAGAAA
ATTGCGATATATTTAATGAG

Product: putative ferredoxin reductase

Products: oxidized ferredoxin; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MGDFVIIGSGVAGHRAAAELHRLDPSQSVYVVGTEPGAPYDRPPLSKEYLKTTEPVRPDLGPAEIYKSNVTLRDGLTATS
IDRPNQTIALSDGSSIQYDRLLLATGSRLRHLDLPNIDPARVFYLRTILDAQRLRTALDNHLRIAIIGGGFVGLEIAAAA
KRNGCTVTLLERAPELLSRGATPPLAEFARKMHSRQDVRIFLGINISEAFQDGSDIVLRWPEGEVRSDILVVGVGIIPNI
ELALQCGLATADGILVDQQCRTSDEWIFAAGEVTSYPVDRLGVHIRTESWSSASAQGTVAATSMLGRNGMFNELPWFWSD
QYDCNIQCLGLPKRASRFHQIGDLNSESWLRIGVDDAGSLVGAESVNKGREMSALRRADRRGEPIPTALIKDSILVQSCS
TVGDLGAGSASLNVNVRTQ

Sequences:

>Translated_419_residues
MGDFVIIGSGVAGHRAAAELHRLDPSQSVYVVGTEPGAPYDRPPLSKEYLKTTEPVRPDLGPAEIYKSNVTLRDGLTATS
IDRPNQTIALSDGSSIQYDRLLLATGSRLRHLDLPNIDPARVFYLRTILDAQRLRTALDNHLRIAIIGGGFVGLEIAAAA
KRNGCTVTLLERAPELLSRGATPPLAEFARKMHSRQDVRIFLGINISEAFQDGSDIVLRWPEGEVRSDILVVGVGIIPNI
ELALQCGLATADGILVDQQCRTSDEWIFAAGEVTSYPVDRLGVHIRTESWSSASAQGTVAATSMLGRNGMFNELPWFWSD
QYDCNIQCLGLPKRASRFHQIGDLNSESWLRIGVDDAGSLVGAESVNKGREMSALRRADRRGEPIPTALIKDSILVQSCS
TVGDLGAGSASLNVNVRTQ
>Mature_418_residues
GDFVIIGSGVAGHRAAAELHRLDPSQSVYVVGTEPGAPYDRPPLSKEYLKTTEPVRPDLGPAEIYKSNVTLRDGLTATSI
DRPNQTIALSDGSSIQYDRLLLATGSRLRHLDLPNIDPARVFYLRTILDAQRLRTALDNHLRIAIIGGGFVGLEIAAAAK
RNGCTVTLLERAPELLSRGATPPLAEFARKMHSRQDVRIFLGINISEAFQDGSDIVLRWPEGEVRSDILVVGVGIIPNIE
LALQCGLATADGILVDQQCRTSDEWIFAAGEVTSYPVDRLGVHIRTESWSSASAQGTVAATSMLGRNGMFNELPWFWSDQ
YDCNIQCLGLPKRASRFHQIGDLNSESWLRIGVDDAGSLVGAESVNKGREMSALRRADRRGEPIPTALIKDSILVQSCST
VGDLGAGSASLNVNVRTQ

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=335, Percent_Identity=27.4626865671642, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI21389617, Length=335, Percent_Identity=27.4626865671642, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI226437568, Length=335, Percent_Identity=27.4626865671642, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI4757732, Length=348, Percent_Identity=27.0114942528736, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI22202629, Length=348, Percent_Identity=27.0114942528736, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788892, Length=402, Percent_Identity=35.3233830845771, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI1789765, Length=282, Percent_Identity=32.2695035460993, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1789065, Length=277, Percent_Identity=26.7148014440433, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081763, Length=218, Percent_Identity=27.0642201834862, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=359, Percent_Identity=25.6267409470752, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32564386, Length=341, Percent_Identity=26.3929618768328, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24581020, Length=357, Percent_Identity=25.7703081232493, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28573993, Length=357, Percent_Identity=25.7703081232493, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI281359715, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359713, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639250, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18543267, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639252, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639257, Length=329, Percent_Identity=23.1003039513678, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585130, Length=323, Percent_Identity=20.1238390092879, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3

Molecular weight: Translated: 45407; Mature: 45276

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDFVIIGSGVAGHRAAAELHRLDPSQSVYVVGTEPGAPYDRPPLSKEYLKTTEPVRPDL
CCCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCC
GPAEIYKSNVTLRDGLTATSIDRPNQTIALSDGSSIQYDRLLLATGSRLRHLDLPNIDPA
CHHHHHCCCCEEECCCEEECCCCCCCEEEECCCCCCEECEEEEECCCCEEECCCCCCCHH
RVFYLRTILDAQRLRTALDNHLRIAIIGGGFVGLEIAAAAKRNGCTVTLLERAPELLSRG
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEEHHCCCCCEEEHHHHHHHHHHCC
ATPPLAEFARKMHSRQDVRIFLGINISEAFQDGSDIVLRWPEGEVRSDILVVGVGIIPNI
CCCCHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCE
ELALQCGLATADGILVDQQCRTSDEWIFAAGEVTSYPVDRLGVHIRTESWSSASAQGTVA
EEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHCEEEEEECCCCCCCCCCCHH
ATSMLGRNGMFNELPWFWSDQYDCNIQCLGLPKRASRFHQIGDLNSESWLRIGVDDAGSL
HHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCE
VGAESVNKGREMSALRRADRRGEPIPTALIKDSILVQSCSTVGDLGAGSASLNVNVRTQ
ECHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEC
>Mature Secondary Structure 
GDFVIIGSGVAGHRAAAELHRLDPSQSVYVVGTEPGAPYDRPPLSKEYLKTTEPVRPDL
CCEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCCCCCC
GPAEIYKSNVTLRDGLTATSIDRPNQTIALSDGSSIQYDRLLLATGSRLRHLDLPNIDPA
CHHHHHCCCCEEECCCEEECCCCCCCEEEECCCCCCEECEEEEECCCCEEECCCCCCCHH
RVFYLRTILDAQRLRTALDNHLRIAIIGGGFVGLEIAAAAKRNGCTVTLLERAPELLSRG
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCEEEEEEEEHHCCCCCEEEHHHHHHHHHHCC
ATPPLAEFARKMHSRQDVRIFLGINISEAFQDGSDIVLRWPEGEVRSDILVVGVGIIPNI
CCCCHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCE
ELALQCGLATADGILVDQQCRTSDEWIFAAGEVTSYPVDRLGVHIRTESWSSASAQGTVA
EEEEEECCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHCEEEEEECCCCCCCCCCCHH
ATSMLGRNGMFNELPWFWSDQYDCNIQCLGLPKRASRFHQIGDLNSESWLRIGVDDAGSL
HHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCE
VGAESVNKGREMSALRRADRRGEPIPTALIKDSILVQSCSTVGDLGAGSASLNVNVRTQ
ECHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced ferredoxin; NAD+

Specific reaction: reduced ferredoxin + NAD+ = oxidized ferredoxin + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]