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The map label for this gene is hisS

Identifier: 15603876

GI number: 15603876

Start: 2252862

End: 2254133

Strand: Direct

Name: hisS

Synonym: PM2011

Alternate gene names: 15603876

Gene position: 2252862-2254133 (Clockwise)

Preceding gene: 15603875

Following gene: 15603877

Centisome position: 99.8

GC content: 42.37

Gene sequence:

>1272_bases
GTGGCAAAAACAATTCAAGCAATTCGTGGTATGAATGATTGTTCCCCAACGGAAAGCCCATTATGGCAATGGGTCGAAGG
AAAAGTGCGGTCAGTTTTACAAAATTATGGTTATTCTGAAGTGCGTATGCCAATTGTCGAAAGTACGCCATTATTTGCTC
GTGCGATCGGTGAAGTAACCGATGTAGTATCCAAAGAAATGTACACTTTTTGGGATAACGATGAGCAATTAACTTTGCGT
CCAGAAGGGACAGCAGGTTGTGTACGTGCAGCGATTGAACACGGTTGGATTTATAACCAAGAACAACGTTTATGGTATAT
GGGACCGATGTTCCGTCACGAACGTCCACAAAAAGGACGCTATCGCCAGTTCCACCAAGCAGGTGTTGAAGTGTTCGGTA
TTCCTAATCCGGAAATTGATGCTGAGTTAATTATGCTTACGGCACGTTTATGGAAAGAATTAGGCATTGCTGAGCATGTC
ACCTTGCAACTGAATTCAATTGGTTCATTAGAGGCGCGTAAGAACTATCGTTCTGCTTTAGTCGCTTTTTTACAGCAACA
TGTTGATTTATTAAGTGAAGAAGAAAAAGAACGTTTAGAGAAAAATCCACTGCGTATTTTGGATACAAAAAATCAAGCGT
TACAAGAAGTGCTAAATGATGCGCCAAAACTGTTAGCGTATTTAGATGACGATAGCCGTGAACACTTTGCCCAACTTTGT
GCTTTACTGGATGCGGTGGGGATTCAATATGAAGTGAATCCAAAACTGGTGCGCGGTTTAGATTATTACAACAAAACTGT
TTTTGAGTGGGTGACTTCTGCGTTAGGAGCACAGGGCACTGTTTGTGGTGGTGGTCGCTACGACGGTCTTGTTGAGCAAT
TAGGTGGTCATGCGACGACAGGTGTGGGCTTTGCGATGGGGCTAGAACGCTTAGTGTTATTGGTGCAAGAAGTGAATCAA
AGTATCCGCTTGCCAAGTGCGGTTGATATTTACGTGGTTTACCAAGGCGAAGGGACAACATTAGCGGCATTTCAGTTAGC
TGAAACTCTCCGTACTGAATTGCCACAATTACGTGTGATGACCCACTGCAGTGGTGGTAACTTCAAAAAACAATTTAAAC
GCGCAGATAAAAGTGGTGCGACATTGGCGTTAGTGATTGGTGAAAGTGAAGTGCAAAATCAACAAGTTGTGGTTAAACAC
TTACTTGGTGGAGCCGAGCAACAAACACTTGCATTAGATGATATTGTTGATTACATTAAGAACAATTTTTAA

Upstream 100 bases:

>100_bases
TAATCAATCAATTAGAAGCCAAAATCCGTGCCAAAGTGGCGGCGCAAGATCCCAAAAATCGAATTATTTAAAATAACAAA
AGAAAAAATAGGATAGTATT

Downstream 100 bases:

>100_bases
TCGAGAAGGAAGAGGAAAATGGCTTATACTGCGGAAGAAGAACAAGAGCTAAATGAGATTAAAACATGGTGGAAGGAAAA
TTATAAAACTTTAATCGCCT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAKTIQAIRGMNDCSPTESPLWQWVEGKVRSVLQNYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLR
PEGTAGCVRAAIEHGWIYNQEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIMLTARLWKELGIAEHV
TLQLNSIGSLEARKNYRSALVAFLQQHVDLLSEEEKERLEKNPLRILDTKNQALQEVLNDAPKLLAYLDDDSREHFAQLC
ALLDAVGIQYEVNPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATTGVGFAMGLERLVLLVQEVNQ
SIRLPSAVDIYVVYQGEGTTLAAFQLAETLRTELPQLRVMTHCSGGNFKKQFKRADKSGATLALVIGESEVQNQQVVVKH
LLGGAEQQTLALDDIVDYIKNNF

Sequences:

>Translated_423_residues
MAKTIQAIRGMNDCSPTESPLWQWVEGKVRSVLQNYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLR
PEGTAGCVRAAIEHGWIYNQEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIMLTARLWKELGIAEHV
TLQLNSIGSLEARKNYRSALVAFLQQHVDLLSEEEKERLEKNPLRILDTKNQALQEVLNDAPKLLAYLDDDSREHFAQLC
ALLDAVGIQYEVNPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATTGVGFAMGLERLVLLVQEVNQ
SIRLPSAVDIYVVYQGEGTTLAAFQLAETLRTELPQLRVMTHCSGGNFKKQFKRADKSGATLALVIGESEVQNQQVVVKH
LLGGAEQQTLALDDIVDYIKNNF
>Mature_422_residues
AKTIQAIRGMNDCSPTESPLWQWVEGKVRSVLQNYGYSEVRMPIVESTPLFARAIGEVTDVVSKEMYTFWDNDEQLTLRP
EGTAGCVRAAIEHGWIYNQEQRLWYMGPMFRHERPQKGRYRQFHQAGVEVFGIPNPEIDAELIMLTARLWKELGIAEHVT
LQLNSIGSLEARKNYRSALVAFLQQHVDLLSEEEKERLEKNPLRILDTKNQALQEVLNDAPKLLAYLDDDSREHFAQLCA
LLDAVGIQYEVNPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATTGVGFAMGLERLVLLVQEVNQS
IRLPSAVDIYVVYQGEGTTLAAFQLAETLRTELPQLRVMTHCSGGNFKKQFKRADKSGATLALVIGESEVQNQQVVVKHL
LGGAEQQTLALDDIVDYIKNNF

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=422, Percent_Identity=68.0094786729858, Blast_Score=586, Evalue=1e-168,
Organism=Caenorhabditis elegans, GI71993693, Length=468, Percent_Identity=23.9316239316239, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71993686, Length=468, Percent_Identity=23.9316239316239, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325290, Length=473, Percent_Identity=22.4101479915433, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_PASMU (P57988)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246950.1
- ProteinModelPortal:   P57988
- SMR:   P57988
- GeneID:   1245358
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM2011
- NMPDR:   fig|272843.1.peg.2012
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   PMUL272843:PM2011-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47531; Mature: 47400

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTIQAIRGMNDCSPTESPLWQWVEGKVRSVLQNYGYSEVRMPIVESTPLFARAIGEVT
CCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHCCCCHHHHHHHHHH
DVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIEHGWIYNQEQRLWYMGPMFRHERPQKGR
HHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCCH
YRQFHQAGVEVFGIPNPEIDAELIMLTARLWKELGIAEHVTLQLNSIGSLEARKNYRSAL
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
VAFLQQHVDLLSEEEKERLEKNPLRILDTKNQALQEVLNDAPKLLAYLDDDSREHFAQLC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ALLDAVGIQYEVNPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATT
HHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
GVGFAMGLERLVLLVQEVNQSIRLPSAVDIYVVYQGEGTTLAAFQLAETLRTELPQLRVM
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEE
THCSGGNFKKQFKRADKSGATLALVIGESEVQNQQVVVKHLLGGAEQQTLALDDIVDYIK
EECCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NNF
CCC
>Mature Secondary Structure 
AKTIQAIRGMNDCSPTESPLWQWVEGKVRSVLQNYGYSEVRMPIVESTPLFARAIGEVT
CCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCHHCCCCHHHHHHHHHH
DVVSKEMYTFWDNDEQLTLRPEGTAGCVRAAIEHGWIYNQEQRLWYMGPMFRHERPQKGR
HHHHHHHHEEECCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCCH
YRQFHQAGVEVFGIPNPEIDAELIMLTARLWKELGIAEHVTLQLNSIGSLEARKNYRSAL
HHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
VAFLQQHVDLLSEEEKERLEKNPLRILDTKNQALQEVLNDAPKLLAYLDDDSREHFAQLC
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ALLDAVGIQYEVNPKLVRGLDYYNKTVFEWVTSALGAQGTVCGGGRYDGLVEQLGGHATT
HHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCC
GVGFAMGLERLVLLVQEVNQSIRLPSAVDIYVVYQGEGTTLAAFQLAETLRTELPQLRVM
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHEEE
THCSGGNFKKQFKRADKSGATLALVIGESEVQNQQVVVKHLLGGAEQQTLALDDIVDYIK
EECCCCCHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NNF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100