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The map label for this gene is ispG [H]

Identifier: 15603875

GI number: 15603875

Start: 2251729

End: 2252832

Strand: Direct

Name: ispG [H]

Synonym: PM2010

Alternate gene names: 15603875

Gene position: 2251729-2252832 (Clockwise)

Preceding gene: 15603874

Following gene: 15603876

Centisome position: 99.74

GC content: 45.47

Gene sequence:

>1104_bases
ATGTTAGCCAAACCAACGATTAAGCGTAGAGAATCAACCAAAATCTATGTGGGGAACGTGCCGATTGGTGGGGATGCACC
TATTGCAGTGCAATCCATGACCAATACTAGAACAACTGATGTAGAAGCCACTGTGGCGCAAATTAAAGCGCTGGAACGTG
TTGGTGCGGATATTGTTCGTGTTTCAGTCCCGACAATGGATGCAGCTGAGGCATTTAAGCTCATCAAGCAGCAAGTGAAT
GTGCCTTTAGTAGCGGATATTCATTTTGATTATCGTATTGCGTTAAAAGTGGCGGAGTACGGAGTGGACTGCTTGCGAAT
TAACCCGGGTAATATTGGTCGTGAAGATCGTATCCGTGCAGTTGTCGATTGCGCCAGAGATAACAATATTCCGATCCGTA
TCGGGGTGAATGCGGGATCTTTGGAAAAAGATCTGCAAGAAAAATACGGTGAACCGACGCCCGAAGCGTTATTGGAATCG
GCGTTACGTCATGTGGAAATTCTAGATCGCCTTAATTTCGACCAGTTTAAGGTCAGTGTGAAGGCATCTGATGTGTTTTT
AGCGGTCGAATCCTATCGTTTATTGGCGAAAGCCATCAAACAACCCTTGCATTTAGGCATCACCGAAGCCGGCGGTTTTC
GTGCTGGGGCAGTAAAAAGTGCGGTGGGGTTGGGCATGCTTTTAGCGGAAGGCATTGGCGATACATTACGCATTTCGCTT
GCGGCCGATCCTGTTGAAGAAATCAAAGTGGGCTTTGATATTCTCAAATCATTACGGATCCGCTCTCGTGGTATTAACTT
TATTGCTTGCCCAACTTGTTCACGTCAAGAGTTTGATGTGATTGGCACGGTTAACGCCCTTGAGCAACGCTTAGAAGATA
TTATTACACCAATGGATGTGTCGATTATCGGCTGTGTCGTTAACGGACCGGGTGAAGCACTCGTCTCTGATTTAGGTGTC
ACGGGTGGTAATAAGAAAAGCGGTTATTATTTAGATGGGGAACGCCAAAAAGAGCGCTTTGATAATGATGATCTAATCAA
TCAATTAGAAGCCAAAATCCGTGCCAAAGTGGCGGCGCAAGATCCCAAAAATCGAATTATTTAA

Upstream 100 bases:

>100_bases
AGATCACATATAAAGGCGAAGACTATCCGCTAAAAGTAGATGGTCGTGTCGCTCGATTTAAATTACCACAGTAATGTGGC
TGATAAGGTAGCAAACAATT

Downstream 100 bases:

>100_bases
AATAACAAAAGAAAAAATAGGATAGTATTGTGGCAAAAACAATTCAAGCAATTCGTGGTATGAATGATTGTTCCCCAACG
GAAAGCCCATTATGGCAATG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLAKPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRIRAVVDCARDNNIPIRIGVNAGSLEKDLQEKYGEPTPEALLES
ALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGFRAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGV
TGGNKKSGYYLDGERQKERFDNDDLINQLEAKIRAKVAAQDPKNRII

Sequences:

>Translated_367_residues
MLAKPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRIRAVVDCARDNNIPIRIGVNAGSLEKDLQEKYGEPTPEALLES
ALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGFRAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGV
TGGNKKSGYYLDGERQKERFDNDDLINQLEAKIRAKVAAQDPKNRII
>Mature_367_residues
MLAKPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVN
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRIRAVVDCARDNNIPIRIGVNAGSLEKDLQEKYGEPTPEALLES
ALRHVEILDRLNFDQFKVSVKASDVFLAVESYRLLAKAIKQPLHLGITEAGGFRAGAVKSAVGLGMLLAEGIGDTLRISL
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSDLGV
TGGNKKSGYYLDGERQKERFDNDDLINQLEAKIRAKVAAQDPKNRII

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=366, Percent_Identity=87.7049180327869, Blast_Score=644, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39970; Mature: 39970

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKALERVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRIRA
EECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCHHHHHHH
VVDCARDNNIPIRIGVNAGSLEKDLQEKYGEPTPEALLESALRHVEILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKAIKQPLHLGITEAGGFRAGAVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSDLGVTGGNKKSGYYLDGERQKERFDNDDLINQLEAKIRAKVAAQ
EEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCC
DPKNRII
CCCCCCC
>Mature Secondary Structure
MLAKPTIKRRESTKIYVGNVPIGGDAPIAVQSMTNTRTTDVEATVAQIKALERVGADIVR
CCCCCCCCCCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
VSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGREDRIRA
EECCCCCHHHHHHHHHHHCCCCEEEEEECCEEEEEEEHHCCCEEEEECCCCCCHHHHHHH
VVDCARDNNIPIRIGVNAGSLEKDLQEKYGEPTPEALLESALRHVEILDRLNFDQFKVSV
HHHHHCCCCCCEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFLAVESYRLLAKAIKQPLHLGITEAGGFRAGAVKSAVGLGMLLAEGIGDTLRISL
EECEEEEEHHHHHHHHHHHHCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQEFDVIGTVNALEQRLEDIITPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSDLGVTGGNKKSGYYLDGERQKERFDNDDLINQLEAKIRAKVAAQ
EEEEEEECCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHCCCHHHHHHHHHHHHHHHHCC
DPKNRII
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA