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The map label for this gene is rlmN

Identifier: 15603872

GI number: 15603872

Start: 2248855

End: 2250039

Strand: Direct

Name: rlmN

Synonym: PM2007

Alternate gene names: 15603872

Gene position: 2248855-2250039 (Clockwise)

Preceding gene: 15603871

Following gene: 15603873

Centisome position: 99.62

GC content: 41.43

Gene sequence:

>1185_bases
ATGTTAGAACAACAAACCTGTGCTGAAAAAGCAGCAAATACAGTCAACACGGTTGAATTATCAACCGACCAAGCAAAAAA
AATTAACTTAATGAATTTAACACGCCAGCAAATGCGTGAGTTCTTTAAAGAATTGGGCGAAAAACCGTTTCGTGCCGATC
AGTTAGTGAAATGGATTTATCATTTTGGCGAAGATAACTTCGACAATATGACCAATATTAACAAGAAGTTAAGAGATAAA
TTAAAACAAGTAGCTGAAATTAAAGCCCCTGAAGTCGCGGTGGAGCAACGTTCTGCTGACGGTACCATTAAATGGGCAAT
GCAAGTGGGCGATCAGCAAGTGGAAACCGTGTATATTCCAGAAGCAGATCGTGCCACCTTATGTGTCTCTTCACAAGTAG
GTTGTGCGTTGGCTTGTACGTTCTGTTCGACTGCACAACAAGGGTTTAACCGTAACTTGACGGTGTCTGAAATTATTGGT
CAAGTTTGGCGCGCCTCAAAAATCATTGGTAATTTTGGGGTAACCGGTGTGCGTCCGATCACCAACGTGGTGATGATGGG
CATGGGTGAGCCACTGTTAAACGTGGCAAATGTGGTGCCTGCGATGGAAATCATGTTAGATGATTTTGCGTATGGTTTAT
CAAAACGTCGAGTGACGTTATCCACTTCGGGTGTGGTACCTGCGTTAGATAAATTAAGTGAAATGATTGATGTGGCGTTG
GCGATTTCGTTACATGCACCGAATGATGAACTGCGTGATGAAATTGTGCCAATCAATAAAAAATATAACATCAAAATGTT
GATGGATTCGGTAAACCGTTATTTAAGCGTGTCGAATGCAAATCACGGTAAAGTAACTATTGAATATGTAATGTTAGATC
ATGTTAATGATGGTGTGGAGCACGCACATCAGTTAGCACAGGTGTTAAAAAATACACCATGTAAGATCAATTTGATCCCT
TGGAATCCGTTCCCAGAAGCACCTTATGCGAAAAGCTCAAACTCGCGTATTGATCGTTTCCAAAAAACCTTGATGGAATA
TGGGTTCACCGTGATTGTACGTAAAACCCGTGGCGATGATATTGATGCCGCCTGTGGGCAATTAGCCGGTGATGTAATTG
ACCGAACAAAACGTACGGCACAGAAAAAGCAGTTTGGACAAGAAATTGCAGTGAGAAATCATTAA

Upstream 100 bases:

>100_bases
TTTGGCGAAATTGCTAGATTAATGCGCACTAACTCTGTACAATCGCAACTCATTTTTTATAGGCGATTATCTAGGCCGCA
ATGTGTAACAACGAGGCGTT

Downstream 100 bases:

>100_bases
GACAGTCTTGATGGCTGAGGACGTAAAAAGGAGAAAAAATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCT
CTTTTTCTTTCTGCCTGTGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MLEQQTCAEKAANTVNTVELSTDQAKKINLMNLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLRDK
LKQVAEIKAPEVAVEQRSADGTIKWAMQVGDQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIG
QVWRASKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKLSEMIDVAL
AISLHAPNDELRDEIVPINKKYNIKMLMDSVNRYLSVSNANHGKVTIEYVMLDHVNDGVEHAHQLAQVLKNTPCKINLIP
WNPFPEAPYAKSSNSRIDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTAQKKQFGQEIAVRNH

Sequences:

>Translated_394_residues
MLEQQTCAEKAANTVNTVELSTDQAKKINLMNLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLRDK
LKQVAEIKAPEVAVEQRSADGTIKWAMQVGDQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIG
QVWRASKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKLSEMIDVAL
AISLHAPNDELRDEIVPINKKYNIKMLMDSVNRYLSVSNANHGKVTIEYVMLDHVNDGVEHAHQLAQVLKNTPCKINLIP
WNPFPEAPYAKSSNSRIDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTAQKKQFGQEIAVRNH
>Mature_394_residues
MLEQQTCAEKAANTVNTVELSTDQAKKINLMNLTRQQMREFFKELGEKPFRADQLVKWIYHFGEDNFDNMTNINKKLRDK
LKQVAEIKAPEVAVEQRSADGTIKWAMQVGDQQVETVYIPEADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIG
QVWRASKIIGNFGVTGVRPITNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKLSEMIDVAL
AISLHAPNDELRDEIVPINKKYNIKMLMDSVNRYLSVSNANHGKVTIEYVMLDHVNDGVEHAHQLAQVLKNTPCKINLIP
WNPFPEAPYAKSSNSRIDRFQKTLMEYGFTVIVRKTRGDDIDAACGQLAGDVIDRTKRTAQKKQFGQEIAVRNH

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=376, Percent_Identity=79.2553191489362, Blast_Score=632, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_PASMU (Q9CJJ8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246946.1
- ProteinModelPortal:   Q9CJJ8
- GeneID:   1245354
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM2007
- NMPDR:   fig|272843.1.peg.2008
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK11194
- BioCyc:   PMUL272843:PM2007-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 44094; Mature: 44094

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQQTCAEKAANTVNTVELSTDQAKKINLMNLTRQQMREFFKELGEKPFRADQLVKWIY
CCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HFGEDNFDNMTNINKKLRDKLKQVAEIKAPEVAVEQRSADGTIKWAMQVGDQQVETVYIP
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCEEEEECC
EADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGVTGVRPI
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
TNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKLSEMIDVAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHH
AISLHAPNDELRDEIVPINKKYNIKMLMDSVNRYLSVSNANHGKVTIEYVMLDHVNDGVE
HEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEEEEECCCHHHH
HAHQLAQVLKNTPCKINLIPWNPFPEAPYAKSSNSRIDRFQKTLMEYGFTVIVRKTRGDD
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
IDAACGQLAGDVIDRTKRTAQKKQFGQEIAVRNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLEQQTCAEKAANTVNTVELSTDQAKKINLMNLTRQQMREFFKELGEKPFRADQLVKWIY
CCCHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
HFGEDNFDNMTNINKKLRDKLKQVAEIKAPEVAVEQRSADGTIKWAMQVGDQQVETVYIP
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCEEEEECCCCCCEEEEECC
EADRATLCVSSQVGCALACTFCSTAQQGFNRNLTVSEIIGQVWRASKIIGNFGVTGVRPI
CCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
TNVVMMGMGEPLLNVANVVPAMEIMLDDFAYGLSKRRVTLSTSGVVPALDKLSEMIDVAL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHH
AISLHAPNDELRDEIVPINKKYNIKMLMDSVNRYLSVSNANHGKVTIEYVMLDHVNDGVE
HEEEECCCHHHHHHHCCCCCCCCHHHHHHHHHHHEEECCCCCCEEEEEEEEEECCCHHHH
HAHQLAQVLKNTPCKINLIPWNPFPEAPYAKSSNSRIDRFQKTLMEYGFTVIVRKTRGDD
HHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCC
IDAACGQLAGDVIDRTKRTAQKKQFGQEIAVRNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100