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The map label for this gene is rnhB

Identifier: 15603863

GI number: 15603863

Start: 2239228

End: 2239821

Strand: Direct

Name: rnhB

Synonym: PM1998

Alternate gene names: 15603863

Gene position: 2239228-2239821 (Clockwise)

Preceding gene: 15603862

Following gene: 15603864

Centisome position: 99.19

GC content: 44.61

Gene sequence:

>594_bases
ATGGTATTTGAATATCCCAAAGGGGTTGAACTCATTGCAGGTGTGGATGAAGTCGGGCGCGGACCTTTAGTGGGCGCGGT
TGTGACGGCAGCGGTGATTTTAGATCCTCATCAACCTATTTTAGGTCTCAATGACTCGAAAAAACTGTCTGAAAAAAAAC
GCCTTTTGTTAGCCGAAGAAATTAAGCAAAAAGCCTTAGCTTGGAGTTTGGGGCGTGCAGAAGCAGAGGAAATTGATCAA
TTAAATATTTTGCATGCCACCATGTTAGCGATGAAAAGAGCGGTCGAAAATTTGAAAATTCAACCGCACTTTGTGTTAGT
GGATGGCAATCGTGTGCCAGAGCTTATGATCCCAGCACAAGCGATTGTGAAAGGTGATGGACTGGTGGCAGAAATCAGCG
CTGCTTCCATTTTGGCGAAAGTCGCACGCGATCAAGAAATGGCTGAGTTAGATAAACGCTATCCGGAATATGCCTTTGCG
CAACATAAAGGTTACCCAACGGCGTTGCATTTAGCCAAATTAGCCGAGTTGGGACCTTTAGCCCAACATAGACGTAGTTT
TGCGCCTGTGCGAAAATTACTCAATACTTTATAA

Upstream 100 bases:

>100_bases
ATTCAACGCTTTACGGATTTACACAAGTTGATCCAATGTGATGCAGATAAACAAGCGGCACAAGCTGTGATTGCATTACT
CGAACAAGAGGATAAATAAC

Downstream 100 bases:

>100_bases
TTCATCTATTTATTTTGTGAGGAGTTTAACGTGGAGCTCAGCTTACAAGCACCTTTCTGGACAAGTTCGTTATTTTGGGT
CTTACTGCTCTTATTTGGCG

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 197; Mature: 197

Protein sequence:

>197_residues
MVFEYPKGVELIAGVDEVGRGPLVGAVVTAAVILDPHQPILGLNDSKKLSEKKRLLLAEEIKQKALAWSLGRAEAEEIDQ
LNILHATMLAMKRAVENLKIQPHFVLVDGNRVPELMIPAQAIVKGDGLVAEISAASILAKVARDQEMAELDKRYPEYAFA
QHKGYPTALHLAKLAELGPLAQHRRSFAPVRKLLNTL

Sequences:

>Translated_197_residues
MVFEYPKGVELIAGVDEVGRGPLVGAVVTAAVILDPHQPILGLNDSKKLSEKKRLLLAEEIKQKALAWSLGRAEAEEIDQ
LNILHATMLAMKRAVENLKIQPHFVLVDGNRVPELMIPAQAIVKGDGLVAEISAASILAKVARDQEMAELDKRYPEYAFA
QHKGYPTALHLAKLAELGPLAQHRRSFAPVRKLLNTL
>Mature_197_residues
MVFEYPKGVELIAGVDEVGRGPLVGAVVTAAVILDPHQPILGLNDSKKLSEKKRLLLAEEIKQKALAWSLGRAEAEEIDQ
LNILHATMLAMKRAVENLKIQPHFVLVDGNRVPELMIPAQAIVKGDGLVAEISAASILAKVARDQEMAELDKRYPEYAFA
QHKGYPTALHLAKLAELGPLAQHRRSFAPVRKLLNTL

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=193, Percent_Identity=72.020725388601, Blast_Score=276, Evalue=7e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PASMU (P57986)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246937.1
- ProteinModelPortal:   P57986
- SMR:   P57986
- GeneID:   1245345
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1998
- NMPDR:   fig|272843.1.peg.1999
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   PMUL272843:PM1998-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21590; Mature: 21590

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFEYPKGVELIAGVDEVGRGPLVGAVVTAAVILDPHQPILGLNDSKKLSEKKRLLLAEE
CCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
IKQKALAWSLGRAEAEEIDQLNILHATMLAMKRAVENLKIQPHFVLVDGNRVPELMIPAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCHH
AIVKGDGLVAEISAASILAKVARDQEMAELDKRYPEYAFAQHKGYPTALHLAKLAELGPL
HHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCHH
AQHRRSFAPVRKLLNTL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVFEYPKGVELIAGVDEVGRGPLVGAVVTAAVILDPHQPILGLNDSKKLSEKKRLLLAEE
CCCCCCCCCHHHCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
IKQKALAWSLGRAEAEEIDQLNILHATMLAMKRAVENLKIQPHFVLVDGNRVPELMIPAQ
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHCCHH
AIVKGDGLVAEISAASILAKVARDQEMAELDKRYPEYAFAQHKGYPTALHLAKLAELGPL
HHHCCCCCEEHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHCHH
AQHRRSFAPVRKLLNTL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100