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The map label for this gene is tsf

Identifier: 15603850

GI number: 15603850

Start: 2225753

End: 2226601

Strand: Direct

Name: tsf

Synonym: PM1985

Alternate gene names: 15603850

Gene position: 2225753-2226601 (Clockwise)

Preceding gene: 161723302

Following gene: 15603851

Centisome position: 98.59

GC content: 42.99

Gene sequence:

>849_bases
ATGGCTGAAATCACAGCATCATTAGTAAAAGAACTTCGTGAGCGTACTGGCGCAGGTATGATGGAATGTAAAAAAGCGTT
AGTTGAAGCAAATGGTGATATCGAATTAGCGATCGATAACATGCGTAAATCAGGTCAAGCAAAAGCGGCTAAGAAAGCAG
GTCGTGTGGCAGCAGAAGGGGTTATCCTTGCTCGTATTGGCGCTGGTTTCGGTGTGTTAGTGGAAATGAACTGTGAAACT
GACTTCGTGGCAAAAGATGCTGGCTTCTTAGGTTTAGCGAACGAAGTAGCAGACTATGCATTAGCGAACAAAGGCGTGAC
TATCGAAGCACTTCAAGCACAATTTGAAGAAAAACGTGCAACGCTTGTGGCGAAAATTGGTGAAAACATGAATATTCGTC
GCGTACAATTTTTAGATGGCGATGTCGTTGGTTCATACTTACACGGCGCCAAAATTGGTGTATTAGTTGCGGGTAAAAAT
GCAGACGAAGATTTATTGAAAAAAATCGCGATGCATGTTGCAGCGAGCCGTCCTGAGTTTGTTAAACCAGAAGATGTGTC
AGCAGATGTTGTAGCAAAAGAGCGTGAAATCCAAGTGGCAATCGCGATGGAATCAGGTAAACCACGTGAAATCGCAGAAA
AAATGGTTGAAGGCCGTATGAAGAAATTCACTGGTGAAGTGTCATTGACAGGTCAACCATTCGTAATGGATCCAGCTAAA
TCAGTAGGCGAATTCTTAAAAGAAAGCGGTGCTGATGTGACTAACTTCGTTCGTTTCGAAGTGGGTGAAGGTATCGAAAA
AGTTGAATCTGACTTTGCAGCAGAAGTTGCAGCGATGCAAAAAATCTAA

Upstream 100 bases:

>100_bases
GATTAATTGGTTAGCAGGGGGCTGAATTAAGTTCCCTGCTTTTTTATGTGAAAGTGCGGTTAAGAAATTGCACAAAATCA
AGCGGACAGAGGATTAAAAA

Downstream 100 bases:

>100_bases
TTTCTAAAAAATAGGCGAGGTAACTCGCCTTTTCTTTATAAGAGAGATGGATAGCGGCACTCGCCTCTCTTTGTGTATAA
GATGTCAGAGTGCAGCGGAA

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCET
DFVAKDAGFLGLANEVADYALANKGVTIEALQAQFEEKRATLVAKIGENMNIRRVQFLDGDVVGSYLHGAKIGVLVAGKN
ADEDLLKKIAMHVAASRPEFVKPEDVSADVVAKEREIQVAIAMESGKPREIAEKMVEGRMKKFTGEVSLTGQPFVMDPAK
SVGEFLKESGADVTNFVRFEVGEGIEKVESDFAAEVAAMQKI

Sequences:

>Translated_282_residues
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCET
DFVAKDAGFLGLANEVADYALANKGVTIEALQAQFEEKRATLVAKIGENMNIRRVQFLDGDVVGSYLHGAKIGVLVAGKN
ADEDLLKKIAMHVAASRPEFVKPEDVSADVVAKEREIQVAIAMESGKPREIAEKMVEGRMKKFTGEVSLTGQPFVMDPAK
SVGEFLKESGADVTNFVRFEVGEGIEKVESDFAAEVAAMQKI
>Mature_281_residues
AEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEGVILARIGAGFGVLVEMNCETD
FVAKDAGFLGLANEVADYALANKGVTIEALQAQFEEKRATLVAKIGENMNIRRVQFLDGDVVGSYLHGAKIGVLVAGKNA
DEDLLKKIAMHVAASRPEFVKPEDVSADVVAKEREIQVAIAMESGKPREIAEKMVEGRMKKFTGEVSLTGQPFVMDPAKS
VGEFLKESGADVTNFVRFEVGEGIEKVESDFAAEVAAMQKI

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=281, Percent_Identity=74.3772241992883, Blast_Score=424, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17561440, Length=293, Percent_Identity=26.962457337884, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19921466, Length=288, Percent_Identity=30.2083333333333, Blast_Score=91, Evalue=6e-19,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_PASMU (P57983)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246924.1
- ProteinModelPortal:   P57983
- SMR:   P57983
- PRIDE:   P57983
- GeneID:   1245332
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1985
- NMPDR:   fig|272843.1.peg.1986
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   PMUL272843:PM1985-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 30209; Mature: 30078

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEG
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
VILARIGAGFGVLVEMNCETDFVAKDAGFLGLANEVADYALANKGVTIEALQAQFEEKRA
EEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TLVAKIGENMNIRRVQFLDGDVVGSYLHGAKIGVLVAGKNADEDLLKKIAMHVAASRPEF
HHHHHHCCCCCEEEEEEECCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
VKPEDVSADVVAKEREIQVAIAMESGKPREIAEKMVEGRMKKFTGEVSLTGQPFVMDPAK
CCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECCHH
SVGEFLKESGADVTNFVRFEVGEGIEKVESDFAAEVAAMQKI
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEITASLVKELRERTGAGMMECKKALVEANGDIELAIDNMRKSGQAKAAKKAGRVAAEG
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCC
VILARIGAGFGVLVEMNCETDFVAKDAGFLGLANEVADYALANKGVTIEALQAQFEEKRA
EEEEEECCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
TLVAKIGENMNIRRVQFLDGDVVGSYLHGAKIGVLVAGKNADEDLLKKIAMHVAASRPEF
HHHHHHCCCCCEEEEEEECCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCC
VKPEDVSADVVAKEREIQVAIAMESGKPREIAEKMVEGRMKKFTGEVSLTGQPFVMDPAK
CCCCCCCHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECCHH
SVGEFLKESGADVTNFVRFEVGEGIEKVESDFAAEVAAMQKI
HHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100