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The map label for this gene is tig

Identifier: 15603840

GI number: 15603840

Start: 2210473

End: 2211771

Strand: Direct

Name: tig

Synonym: PM1975

Alternate gene names: 15603840

Gene position: 2210473-2211771 (Clockwise)

Preceding gene: 15603839

Following gene: 15603841

Centisome position: 97.92

GC content: 41.26

Gene sequence:

>1299_bases
ATGTCATTTACTATTGAAACAACTCAAGGTCTTGAGCGTCGTGTTCAAATCACCGTTCCAGCTGACACTGTTGAAGGTGC
CGTACGTGAAGAATTAAAACGTGTTGCAAAAACCGCACGTGTTGACGGTTTCCGTAAAGGTAAAGTTCCACCGCATATCA
TTGAAAAACGTTTTGGTGCCTCTGTGCGTCATGATGTGCTAGGTGATGTATTACAAAGCCACTTCTTTAACGTTGTGATG
GAGCAAAAAGTCAATTTAGCGGGTCGTCCAGCTTTTGCTGTAGAACAATTTGAAGCAGGTAAAGATTTAGTATTTACTGC
AACTTTTGAAGTTTATCCAGAAGTAGCTTTACAAGGTTTAGACCAAATTAAAGTGGAAAAGCCAGTTGTTGAAATTAGCG
AAGCTGACATTGATAAAATGATCGATGTATTACGTAAACAGCAAGCAACTTGGGCGGAAACAGCAGAAGCCGCAAGCGCA
GAAGACCGTGTTGTGATTGATTTTGTGGGTTCTGTAGATGGCGAAGTATTTGAAGGTGGTAAAGCTGAAGACTTCACGTT
ATTCATGGGGCAAGGTCGAATGATCCCAGGCTTTGAAGAGGGGATTGTGGGGCATAAAGCCGGTGAACAATTTGATATTG
ACGTGACTTTCCCTGAAGAATATCACGCAGAAAACTTAAAAGGTAAAGCGGCAAAATTTGCGATTACCTTGAAAAAAGTC
GAAGTGATGGTATTACCAGAATTAACCGATGAGTTTGTGGCGAAATTCGGTCCAAACACCAAAACAGTAGATGATTTACG
TAACGAAATTCGTAAAAACATGCAACGTGAATTGAAAAATGCGTTGACCGCAAAAGTAAAAGGTCAAGTGATTGATGGTT
TACTTGCACAAAATGCGATTGATGTACCTGCTTCTGCAGTAGAACAAGAAATCGAAGTATTGCGTGGTCAAGCTGCACAA
CGTTTCGGCGGCAACAAAGAGCAAGCTGCACAATTACCACGTGAATTGTTTGAAGAACAAGCAAAACGCCGTGTTCAAGT
TGGCTTATTATTAGCGGAAGTGATTGCATCTCATGAGTTAAAAGTCGATGAAGCACGTGCAAAAACCATGATCGAAGAGA
TTGCCTCTGCGTATGAGCAACCGGCTGAAGTGGTTGAATATTACAGCAAGAACAAAGAGTTAATGAATAATATTCGCAAT
GTTGTGTTGGAAGAACAAGCTGTTGATGCGGTTCTTGCAAAAGCACAAGTGACGGAAAAAGCAGCCTCTTTTGATGATGT
GATGAATCCACAAGCGTAA

Upstream 100 bases:

>100_bases
TTTAAACCGCTATAATTCGTCGGTTATTTTTAACTTTAATTTTTACTAACGTGTTCGGTGCGTGCATTCGCCGAGTGCAA
ATTCATTTTGAGGTAACATA

Downstream 100 bases:

>100_bases
TTTGCTTTAAGCGTCATATCCCTTTTAAGTGCGGTCAAAATGTTGAATATTTTGACCGCACTTTTTTATCTTGTGTAAAT
ATTCAATTTAGTGAAATCGG

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MSFTIETTQGLERRVQITVPADTVEGAVREELKRVAKTARVDGFRKGKVPPHIIEKRFGASVRHDVLGDVLQSHFFNVVM
EQKVNLAGRPAFAVEQFEAGKDLVFTATFEVYPEVALQGLDQIKVEKPVVEISEADIDKMIDVLRKQQATWAETAEAASA
EDRVVIDFVGSVDGEVFEGGKAEDFTLFMGQGRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
EVMVLPELTDEFVAKFGPNTKTVDDLRNEIRKNMQRELKNALTAKVKGQVIDGLLAQNAIDVPASAVEQEIEVLRGQAAQ
RFGGNKEQAAQLPRELFEEQAKRRVQVGLLLAEVIASHELKVDEARAKTMIEEIASAYEQPAEVVEYYSKNKELMNNIRN
VVLEEQAVDAVLAKAQVTEKAASFDDVMNPQA

Sequences:

>Translated_432_residues
MSFTIETTQGLERRVQITVPADTVEGAVREELKRVAKTARVDGFRKGKVPPHIIEKRFGASVRHDVLGDVLQSHFFNVVM
EQKVNLAGRPAFAVEQFEAGKDLVFTATFEVYPEVALQGLDQIKVEKPVVEISEADIDKMIDVLRKQQATWAETAEAASA
EDRVVIDFVGSVDGEVFEGGKAEDFTLFMGQGRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
EVMVLPELTDEFVAKFGPNTKTVDDLRNEIRKNMQRELKNALTAKVKGQVIDGLLAQNAIDVPASAVEQEIEVLRGQAAQ
RFGGNKEQAAQLPRELFEEQAKRRVQVGLLLAEVIASHELKVDEARAKTMIEEIASAYEQPAEVVEYYSKNKELMNNIRN
VVLEEQAVDAVLAKAQVTEKAASFDDVMNPQA
>Mature_431_residues
SFTIETTQGLERRVQITVPADTVEGAVREELKRVAKTARVDGFRKGKVPPHIIEKRFGASVRHDVLGDVLQSHFFNVVME
QKVNLAGRPAFAVEQFEAGKDLVFTATFEVYPEVALQGLDQIKVEKPVVEISEADIDKMIDVLRKQQATWAETAEAASAE
DRVVIDFVGSVDGEVFEGGKAEDFTLFMGQGRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKVE
VMVLPELTDEFVAKFGPNTKTVDDLRNEIRKNMQRELKNALTAKVKGQVIDGLLAQNAIDVPASAVEQEIEVLRGQAAQR
FGGNKEQAAQLPRELFEEQAKRRVQVGLLLAEVIASHELKVDEARAKTMIEEIASAYEQPAEVVEYYSKNKELMNNIRNV
VLEEQAVDAVLAKAQVTEKAASFDDVMNPQA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=432, Percent_Identity=64.8148148148148, Blast_Score=578, Evalue=1e-166,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_PASMU (P57980)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246914.1
- ProteinModelPortal:   P57980
- SMR:   P57980
- GeneID:   1245322
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1975
- NMPDR:   fig|272843.1.peg.1976
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   PRK01490
- BioCyc:   PMUL272843:PM1975-MONOMER
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 47897; Mature: 47766

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFTIETTQGLERRVQITVPADTVEGAVREELKRVAKTARVDGFRKGKVPPHIIEKRFGA
CCEEECCHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
SVRHDVLGDVLQSHFFNVVMEQKVNLAGRPAFAVEQFEAGKDLVFTATFEVYPEVALQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCCH
DQIKVEKPVVEISEADIDKMIDVLRKQQATWAETAEAASAEDRVVIDFVGSVDGEVFEGG
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
KAEDFTLFMGQGRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
CCCCEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHHEEEEEEHE
EVMVLPELTDEFVAKFGPNTKTVDDLRNEIRKNMQRELKNALTAKVKGQVIDGLLAQNAI
EEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPASAVEQEIEVLRGQAAQRFGGNKEQAAQLPRELFEEQAKRRVQVGLLLAEVIASHEL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVDEARAKTMIEEIASAYEQPAEVVEYYSKNKELMNNIRNVVLEEQAVDAVLAKAQVTEK
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASFDDVMNPQA
HHHHHHHCCCCC
>Mature Secondary Structure 
SFTIETTQGLERRVQITVPADTVEGAVREELKRVAKTARVDGFRKGKVPPHIIEKRFGA
CEEECCHHCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCC
SVRHDVLGDVLQSHFFNVVMEQKVNLAGRPAFAVEQFEAGKDLVFTATFEVYPEVALQGL
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHCCH
DQIKVEKPVVEISEADIDKMIDVLRKQQATWAETAEAASAEDRVVIDFVGSVDGEVFEGG
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
KAEDFTLFMGQGRMIPGFEEGIVGHKAGEQFDIDVTFPEEYHAENLKGKAAKFAITLKKV
CCCCEEEEECCCCCCCCHHHCCCCCCCCCEEEEEEECCHHHHHHCCCCCHHHEEEEEEHE
EVMVLPELTDEFVAKFGPNTKTVDDLRNEIRKNMQRELKNALTAKVKGQVIDGLLAQNAI
EEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DVPASAVEQEIEVLRGQAAQRFGGNKEQAAQLPRELFEEQAKRRVQVGLLLAEVIASHEL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KVDEARAKTMIEEIASAYEQPAEVVEYYSKNKELMNNIRNVVLEEQAVDAVLAKAQVTEK
CHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASFDDVMNPQA
HHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100