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The map label for this gene is leuA

Identifier: 15603827

GI number: 15603827

Start: 2201575

End: 2203131

Strand: Reverse

Name: leuA

Synonym: PM1962

Alternate gene names: 15603827

Gene position: 2203131-2201575 (Counterclockwise)

Preceding gene: 15603828

Following gene: 15603826

Centisome position: 97.59

GC content: 43.42

Gene sequence:

>1557_bases
ATGGCAGACAATATCATTATTTTTGACACCACATTACGTGATGGCGAACAGGCATTAAAAGCCAGCTTAACGGTAAAAGA
AAAATTACAAATTGCCTTCGCCTTGGAACGTTTAGGCGTGGATGTCATGGAAGTGGGGTTCCCCATTTCGTCTGCTGGAG
ATTTTGAATCGGTGCAAACCATTGCCCGCCATATTAAGCATAGCCGCGTTTGTGCTTTATCTCGCGCTGTGGATAAAGAT
ATTGATGCTGCAGCAGAAGCGTTGAAAGTAGCGGAGGCATTCCGTATTCACACGTTTATTGCCACGTCTGCTTTACATGT
GGAAGCCAAATTACGTCGTACCTTTGAAGATGTGGTCGATATGGCGATCAATGCCGTGAAACGCGCGAGAAACTACACCG
ATGATGTGGAGTTTTCTTGTGAAGATGCAGGGCGTACTGGTGTGGATAATATCTGTCGTATTGTAGAAGCCGCAATTAAA
GCAGGTGCCACGACTGTCAATATTCCCGATACTGTCGGCTACTGTTTGCCATATCAATACGGCGATATTATTGCGAATGT
GATGAATCGTGTACCAAACATTGATAAAGCGATTATTTCTGTGCATTGCCACAATGATTTAGGGATGGCAACTGCCAATT
CATTAACAGCGGTTCAAAATGGCGCACGACAAATTGAATGTACCATTAACGGGATCGGTGAACGTGCCGGTAATACCGCA
TTAGAAGAAGTAGTGATGGCAATTAAAACGCGCCAAGATATGTTCAATGGTTTAGATACTCGCATTAACACACAAGAAAT
TCACCGTGTCAGCCAAATGGTGAGCCAACTTTGTAATATGCCAATCCAACCGAATAAAGCGATTGTGGGATCCAATGCCT
TTGCCCATTCTTCAGGTATCCACCAAGATGGTATGGTAAAACATAAAAATACTTATGAAATTATGTCGCCTGAAAGCATT
GGCTTGAAAAAAGAAAAATTGAACTTAACCGCGCGTTCAGGTCGTGCTGCGGTGAAAAACCATATGAGCGAGATGGGCTA
CCAAGAAAGTGATTACGATTTAGATAAACTCTATGAAGCATTCTTGAAATTGGCGGACAAAAAAGGGCAAGTGTTCGACT
ATGATTTAGAAGCGCTTGCGTTTATTGATATGCAACAAGGCGATGAAGATCGCTTAACCTTAGACGTGATCACTTCACAA
TGTATCAGCCACTTACCCGCATCTGCCTTTGTGCAAGTGGAATTAGACGGCAAAAAAATGAGCCAAGTGTCTAACGGTGG
TAATGGACCTGTTGATGCGGTTTACAATGCGATTTTAGCGATCACCGGCTTAGAGATGAAAATGCTTAACTACAACTTGA
CGGCTAAAGGTGAAGGTGCCGAAGCTTTGGGGCAAGTAGATATTGTGGTGGAACATGAAGGCAGACGTTTCCACGGCGTG
GGACTTGCGACTGACATTGTGGAATCCTCTGCCAGAGCATTGATTCATGCGATTAATGCCATTTACCGTTCGCAAAAAGT
GGCAGATTTGAAATTACATAAAATTGCTGGCGTGTAA

Upstream 100 bases:

>100_bases
CCCGCACATGTAAGCGGGTCTTTTTTTATGAAAACGAAAAAAGACGACTGTACTTAAATACTCGAAATAGCACAACACGA
ACAACAATAAGGATCCCAAT

Downstream 100 bases:

>100_bases
AAAAACGGATTCAAAACACACCGCACTTTAAACAGACATGATGCCAACATGTGATAAGAAGAAATTCATCCAAGACAAAC
TTCATTAAAAGATCAAAAGG

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQTIARHIKHSRVCALSRAVDKD
IDAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVDMAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIK
AGATTVNIPDTVGYCLPYQYGDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA
LEEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKHKNTYEIMSPESI
GLKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEAFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQ
CISHLPASAFVQVELDGKKMSQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGV
GLATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV

Sequences:

>Translated_518_residues
MADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQTIARHIKHSRVCALSRAVDKD
IDAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVDMAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIK
AGATTVNIPDTVGYCLPYQYGDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA
LEEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKHKNTYEIMSPESI
GLKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEAFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQ
CISHLPASAFVQVELDGKKMSQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGV
GLATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV
>Mature_517_residues
ADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQTIARHIKHSRVCALSRAVDKDI
DAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVDMAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIKA
GATTVNIPDTVGYCLPYQYGDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTAL
EEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGIHQDGMVKHKNTYEIMSPESIG
LKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEAFLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQC
ISHLPASAFVQVELDGKKMSQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGVG
LATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=507, Percent_Identity=67.0611439842209, Blast_Score=701, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=574, Percent_Identity=25.0871080139373, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=25, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=363, Percent_Identity=26.4462809917355, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320019, Length=364, Percent_Identity=26.6483516483516, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24582381, Length=291, Percent_Identity=24.3986254295533, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PASMU (Q9CJN5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246901.1
- ProteinModelPortal:   Q9CJN5
- SMR:   Q9CJN5
- GeneID:   1245309
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1962
- NMPDR:   fig|272843.1.peg.1963
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- ProtClustDB:   PRK00915
- BioCyc:   PMUL272843:PM1962-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56521; Mature: 56390

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQT
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKHSRVCALSRAVDKDIDAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIKAGATTVNIPDTVGYCLPYQY
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCHH
GDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCHH
LEEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC
HQDGMVKHKNTYEIMSPESIGLKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEA
CCCCCEECCCCEEEECCCCCCCCHHHCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHH
FLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQCISHLPASAFVQVELDGKKM
HHHHHCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHH
SQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGV
HHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECC
GLATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC
>Mature Secondary Structure 
ADNIIIFDTTLRDGEQALKASLTVKEKLQIAFALERLGVDVMEVGFPISSAGDFESVQT
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IARHIKHSRVCALSRAVDKDIDAAAEALKVAEAFRIHTFIATSALHVEAKLRRTFEDVVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAINAVKRARNYTDDVEFSCEDAGRTGVDNICRIVEAAIKAGATTVNIPDTVGYCLPYQY
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCHHHHHCCCHH
GDIIANVMNRVPNIDKAIISVHCHNDLGMATANSLTAVQNGARQIECTINGIGERAGNTA
HHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHCCCEEEEEEEECCCCCCCCHH
LEEVVMAIKTRQDMFNGLDTRINTQEIHRVSQMVSQLCNMPIQPNKAIVGSNAFAHSSGI
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCC
HQDGMVKHKNTYEIMSPESIGLKKEKLNLTARSGRAAVKNHMSEMGYQESDYDLDKLYEA
CCCCCEECCCCEEEECCCCCCCCHHHCCEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHH
FLKLADKKGQVFDYDLEALAFIDMQQGDEDRLTLDVITSQCISHLPASAFVQVELDGKKM
HHHHHCCCCCEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCHHH
SQVSNGGNGPVDAVYNAILAITGLEMKMLNYNLTAKGEGAEALGQVDIVVEHEGRRFHGV
HHHCCCCCCCHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHHHCCEEEEEECCCCEEECC
GLATDIVESSARALIHAINAIYRSQKVADLKLHKIAGV
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100