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The map label for this gene is leuC [H]

Identifier: 15603825

GI number: 15603825

Start: 2198639

End: 2200045

Strand: Reverse

Name: leuC [H]

Synonym: PM1960

Alternate gene names: 15603825

Gene position: 2200045-2198639 (Counterclockwise)

Preceding gene: 15603826

Following gene: 15603824

Centisome position: 97.46

GC content: 48.47

Gene sequence:

>1407_bases
ATGTCCAAAACCCTATACGAAAAACTCTTCGACGCACATGTCGTCCACGAAGCCAATGGCGAAACGCCATTGATTTACAT
CAACCGCCATTTAGTGCATGAAGTGACCTCCCCACAAGCCTTTGATGGTTTGCGCGCCATGGGACGTTCCGTTCGTCAAC
CGAGTAAAACCGTTGCTACCATGGATCACAATGTCCCAACTGATAGTCGTGATCTAGCAGGCTCAGGCGAAATGGGACGA
GTGCAAATGGTAGAGTTAGCCAAAAATACTGAACAATTTGGCATCACACTCTACGATATTAACCACATCAATCAAGGTAT
TGTGCATGTCATGGGACCAGAACAAGGTTTAACTCTACCGGGTATGACCATTGTCTGTGGCGACTCACATACCGCTACCC
ATGGCGCCTTTGGGGCATTAGCCTTTGGGATCGGCACCTCCGAAGTTGAGCATGTGCTTGCTACTCAAACCATTAAACAA
GCACGCGCGAAAAAAATGAAAATTGAAGTGCGTGGCAAAGTACGTGACGGGATTAGCGCGAAAGATATCGTGTTAGCCAT
CATCGGTAAAACAACCATGGGCGGTGGCACAGGACACGTTGTGGAATTCTGTGGTGAAGCGATCCGCGATCTTTCCATGG
AAGGACGCATGACCGTCTGCAATATGGCTATCGAGTTAGGTGCGAAATCCGGTATTATTGCGCCCGATGAAACGACGTTT
GCTTACTTAAAAGACAAACCTTACGCGCCGAAAGGCAAAGATTGGGACGAGGCTGTTGCCTATTGGCAAACATTGCATAG
CGATGAAGGGGCTGAATTTGATACCGTTGTCACGCTAGAAGCCAGTGAAATCGAACCACAAGTGACTTGGGGTACCAACC
CAGGACAAGTGATTGGTATTAATCAGCCGATCCCAAATCCAGCGGAAATGTCAGATCCGATTGAGCGTCAATCTGCTGAA
AAAGCCTTAGCCTACATGGATTTACCGCACAGCATTAAATTGACCGATGTCGCCATTGATAAAGTCTTTATTGGCTCTTG
CACAAACTCTCGTATTGAAGATTTGCGTGCAGCAGCAGCGATCGCCAAAGGACGCAAAGTGGCAGACGGCGTACAAGCCT
TGGTCGTACCGGGTTCAGGTTTAGTGCGAGAACAAGCAGAGAAAGAAGGCTTAGATAAAATCTTTATTGAAGCCGGTTTT
GAATGGCGTTTACCGGGTTGTTCTATGTGTCTAGCCATGAATAACGACCGCTTAGCCCCAGGTGAACGTTGCGCCTCCAC
CAGTAACCGTAACTTTGAGGGGCGTCAAGGTCGTGGTGGACGCACTCATTTAGTCAGTCCTGCGATGGCCGCCGCCGCTG
CCGTTTACGGCAAATTTGTCGACATCCGTAACTTGGAATTACATTAA

Upstream 100 bases:

>100_bases
GTCGGGGTGTGTTTTTTTGCTCCTTTTTTGCACAAGCAAAAAAGGAGAGAAAGGCTCAGAATAAAATAATTAAAGCTCAA
ACATCATTAGGAATAACACA

Downstream 100 bases:

>100_bases
CCACATTAACCACATTCCCCTCTTAAACCTAGAGGGGAAAACAGAAAAACTGTTTTAAAACTAACCGCACTTTATTGATA
ACGCTCAGACAAAGCGCCCC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSKTLYEKLFDAHVVHEANGETPLIYINRHLVHEVTSPQAFDGLRAMGRSVRQPSKTVATMDHNVPTDSRDLAGSGEMGR
VQMVELAKNTEQFGITLYDINHINQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTIKQ
ARAKKMKIEVRGKVRDGISAKDIVLAIIGKTTMGGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGIIAPDETTF
AYLKDKPYAPKGKDWDEAVAYWQTLHSDEGAEFDTVVTLEASEIEPQVTWGTNPGQVIGINQPIPNPAEMSDPIERQSAE
KALAYMDLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAAIAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLAPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVYGKFVDIRNLELH

Sequences:

>Translated_468_residues
MSKTLYEKLFDAHVVHEANGETPLIYINRHLVHEVTSPQAFDGLRAMGRSVRQPSKTVATMDHNVPTDSRDLAGSGEMGR
VQMVELAKNTEQFGITLYDINHINQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTIKQ
ARAKKMKIEVRGKVRDGISAKDIVLAIIGKTTMGGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGIIAPDETTF
AYLKDKPYAPKGKDWDEAVAYWQTLHSDEGAEFDTVVTLEASEIEPQVTWGTNPGQVIGINQPIPNPAEMSDPIERQSAE
KALAYMDLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAAIAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGF
EWRLPGCSMCLAMNNDRLAPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVYGKFVDIRNLELH
>Mature_467_residues
SKTLYEKLFDAHVVHEANGETPLIYINRHLVHEVTSPQAFDGLRAMGRSVRQPSKTVATMDHNVPTDSRDLAGSGEMGRV
QMVELAKNTEQFGITLYDINHINQGIVHVMGPEQGLTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTIKQA
RAKKMKIEVRGKVRDGISAKDIVLAIIGKTTMGGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGIIAPDETTFA
YLKDKPYAPKGKDWDEAVAYWQTLHSDEGAEFDTVVTLEASEIEPQVTWGTNPGQVIGINQPIPNPAEMSDPIERQSAEK
ALAYMDLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAAIAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGFE
WRLPGCSMCLAMNNDRLAPGERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVYGKFVDIRNLELH

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=399, Percent_Identity=26.0651629072682, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI8659555, Length=454, Percent_Identity=24.2290748898678, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI41352693, Length=384, Percent_Identity=24.7395833333333, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1786259, Length=466, Percent_Identity=80.6866952789699, Blast_Score=773, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=28.4122562674095, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI2367097, Length=355, Percent_Identity=26.1971830985915, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=401, Percent_Identity=28.428927680798, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25149337, Length=401, Percent_Identity=28.428927680798, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25149342, Length=306, Percent_Identity=29.7385620915033, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=452, Percent_Identity=25.4424778761062, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=59.6602972399151, Blast_Score=572, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6323335, Length=470, Percent_Identity=28.7234042553192, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320440, Length=447, Percent_Identity=26.1744966442953, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6322261, Length=400, Percent_Identity=27.5, Blast_Score=134, Evalue=4e-32,
Organism=Drosophila melanogaster, GI161076999, Length=399, Percent_Identity=26.5664160401003, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI281365315, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17864292, Length=398, Percent_Identity=26.1306532663317, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571643, Length=400, Percent_Identity=27.5, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.3157894736842, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI17137564, Length=456, Percent_Identity=25.4385964912281, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50530; Mature: 50399

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTLYEKLFDAHVVHEANGETPLIYINRHLVHEVTSPQAFDGLRAMGRSVRQPSKTVAT
CCHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCHHEEE
MDHNVPTDSRDLAGSGEMGRVQMVELAKNTEQFGITLYDINHINQGIVHVMGPEQGLTLP
ECCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTIKQARAKKMKIEVRGKVRDGISA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
KDIVLAIIGKTTMGGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGIIAPDETTF
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCEE
AYLKDKPYAPKGKDWDEAVAYWQTLHSDEGAEFDTVVTLEASEIEPQVTWGTNPGQVIGI
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCCCEEEECCCCCEEEEC
NQPIPNPAEMSDPIERQSAEKALAYMDLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
IAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLAP
HHCCCHHHCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCC
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVYGKFVDIRNLELH
CHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEEECCCCC
>Mature Secondary Structure 
SKTLYEKLFDAHVVHEANGETPLIYINRHLVHEVTSPQAFDGLRAMGRSVRQPSKTVAT
CHHHHHHHHHHHEEECCCCCCCEEEEEHHHHHHCCCCHHHHHHHHHHHHHCCCCHHEEE
MDHNVPTDSRDLAGSGEMGRVQMVELAKNTEQFGITLYDINHINQGIVHVMGPEQGLTLP
ECCCCCCCCCCCCCCCCCCCEEEEHHHCCCHHHEEEEEEECCCCCCEEEEECCCCCCCCC
GMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTIKQARAKKMKIEVRGKVRDGISA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCC
KDIVLAIIGKTTMGGGTGHVVEFCGEAIRDLSMEGRMTVCNMAIELGAKSGIIAPDETTF
CCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEHHHHHHCCCCCCCCCCCCEE
AYLKDKPYAPKGKDWDEAVAYWQTLHSDEGAEFDTVVTLEASEIEPQVTWGTNPGQVIGI
EEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEHHCCCCEEEECCCCCEEEEC
NQPIPNPAEMSDPIERQSAEKALAYMDLPHSIKLTDVAIDKVFIGSCTNSRIEDLRAAAA
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHH
IAKGRKVADGVQALVVPGSGLVREQAEKEGLDKIFIEAGFEWRLPGCSMCLAMNNDRLAP
HHCCCHHHCCCEEEEECCCCHHHHHHHHCCCCEEEEECCCEEECCCCEEEEEECCCCCCC
GERCASTSNRNFEGRQGRGGRTHLVSPAMAAAAAVYGKFVDIRNLELH
CHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA