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The map label for this gene is vacB [H]

Identifier: 15603819

GI number: 15603819

Start: 2191692

End: 2194094

Strand: Reverse

Name: vacB [H]

Synonym: PM1954

Alternate gene names: 15603819

Gene position: 2194094-2191692 (Counterclockwise)

Preceding gene: 15603823

Following gene: 15603818

Centisome position: 97.19

GC content: 42.86

Gene sequence:

>2403_bases
ATGGCAAAAATCAAAAACAATCCACATTTGCAAGATCCGAACTATCAAGAGGAACTCGCAAAATATGAGAACCCGATCCC
GAGTCGCGAATTTATTCTTGATACAATTCGTCAACATAATGCACCTATCTCAAAAGAAGAATTACTGACCACATTTGCTA
TTCAAAATGAAGAACAACAAGAAGCCATGCGCCGTCGCTTAAGAGCGATGGAAAATGACGGACAATTGGTTTTCACTAAA
CGTAAACGCTACGCTTTACCGGAAAAACTGGATTTATTAAAAGGGACCGTATTAGGTCATCGTGATGGTTATGGTTTTTT
ACAAGTCGAAGGTAAAGACAGCGATTGGTTTATACCCAACAGCCAAATGCAGCGAGTAATGCACGGTGACTATGTTCTCG
CCCAACCTAGCGGTTTTGATCGCCGTGGTCGCCAAGAAGTGCGGATTGTCCGCGTCTTACAACAGCGTAAAAAACAGATT
GTTGGTCGTTTTTTCTTAGAAGATGGTATTGGTTATGTAGTACCTGACGATAGTCGAATTAATCGCGACATTTTAATTCC
GAATGAACACCGCCAAGGCGCTCGTATGGGACAAGTGGTTGTAGTTGAATTAAAACCACGTACCGCAACATTTAGCCAAC
CTGTTGGAATGATTAGCGAAATTCTCGGTGAAAATATGGCAAAAGGGATGGAGGTGGAAATTGCTATCCGTAATCATGAT
ATTCCCCATGTTTTTCCAGACGCGGTCCTCAAACAAGTTTCTAAACTGAGTGAAGAAGTACCTGAAGAAGCCAAATCAGG
ACGTATTGATTTACGTCATCTTCCGTTAGTCACCATTGATGGTGAAGATGCTCGCGATTTTGACGATGCGGTCTTTTGCC
AGAAAAAACGTGGAGGCGGTTGGAAACTGTGGGTCGCCATTGCCGATGTCAGCTACTATGTTCGTTTACGTACTGCCCTT
GATACCGAAGCACATGCGCGTGGAAACTCGGTATATTTCCCAAATCGTGTTGTTCCTATGCTACCGGAAATCCTCTCCAA
TGGACTTTGTTCATTAAACCCACAAGTCGATCGCTTATGTATGGTCTGTGAGCTGAATGTTTCAGCAAAAGGTAAAATCA
CAGGTTATGAATTTTATGAAGCGGTCATGAACTCTCACGCTCGCTTAACCTATACCAAAGTTGCTCGTATTTTAGAGGAC
GATGAAGAACTTTGTACTCGTTATCAAGCACTGGTTCCCCATTTAAAAGAACTCCATGTAATGTACCAAGCCTTAGTGGA
AGCCCGTCATCAACGTGGCGCCATTGACTTTGAAACCATTGAAAGTAAGTTTGTGTTTAATGAAATGGGGCGAATCGAAC
GGATTGAGCCTGTTATTCGTAATGATGCGCATAAAATCATTGAAGAATGTATGATCCTCGCCAATATTGCGGCGGCAAAC
TTTATGGAAAAACATCAAGAGCCAGCCCTTTTCCGTATTCATGCGGGTCCAAGCGAAGAAAAAGTGACGGGCTTCCGCCA
ATTCCTCAGTGAATGTGGTTTAAGCCTTGAAGGTGGCAGTAAACCAAGTACCGCAGACTACGCTAAATTATTGGAGCAAA
TTCGCGAACGTCCAGATCATGAGTTATTGCAAACCATGTTATTACGTTCGTTAAGTCAAGCCGTTTATCATCCTGACAAT
ATTGGGCACTTTGGGCTTGCACTCGAAGAATATGCGCATTTTACCTCGCCAATTCGCCGTTATCCTGATTTGACTTTGCA
TCGTGGGATTAAATACTTGTTAGCCAAACTAAAAGGCTCGAAACGGAGAACCACTGATACGGGAGGGTATCACTATACAC
TTGATGACATGGATGTATTAGGCGACCACTGCTCCATGACAGAACGACGGGCCGATGATGCCACTCGTGATGTTGCAGAC
TGGCTAAAATGCGAATATATGCAAGATCATGTAGGCGAAGAATTTACAGGTGTGATTTCATCTGTGACAGGTTTTGGCTT
ATTTGTCCGCTTAGATGACCTCTTTATTGATGGCTTAGTCCACATTTCTACCTTAGATAACGACTACTACCAATTTGATC
TCGCTCGACAACGTTTAATCGGTGAAAATAGTGGTATGATCTACCGCATTGGCGATAAGGTAAAAATCCGTGTGGAAGCC
GTTAGCCTTGAACAACGTCAAGTGGATTTTTCTTTAGTGTCGAGTGAGCGTAAACCATTACGAGAAGGCAAAACAGCCAA
AGACAAGCTCAAGAAAAATGCACGCTACGCCGAAAGTCTCGAAAAACAACGCCATAAACGTAAACCAAGCACCAGTAAAA
GTGCGGTAAGAAAAACAAGCGTTGTTAAACCATCAACAACCACGAAAAAAGCCAGTAAGAAATCAGCTAAAAAGAGAAAG
TAG

Upstream 100 bases:

>100_bases
TTCAGTCACGAACAGGTTTTTTACTTGCTTTCAACAAATCAGAGTGTCAAACTTGAGGTCATCAAACGAATGCGTATTTC
CATTCTCAAAGGGAGTCATT

Downstream 100 bases:

>100_bases
ATGAGTGAAAATATTTATGGCATTCATGCAGTAAGTGCCTTTTTAAACAACGCGCCAGAGCGTTTAATTGAAGTTTATGC
ACTCAAAGGGCGCGACGATA

Product: hypothetical protein

Products: NA

Alternate protein names: RNase R; VacB protein homolog [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MAKIKNNPHLQDPNYQEELAKYENPIPSREFILDTIRQHNAPISKEELLTTFAIQNEEQQEAMRRRLRAMENDGQLVFTK
RKRYALPEKLDLLKGTVLGHRDGYGFLQVEGKDSDWFIPNSQMQRVMHGDYVLAQPSGFDRRGRQEVRIVRVLQQRKKQI
VGRFFLEDGIGYVVPDDSRINRDILIPNEHRQGARMGQVVVVELKPRTATFSQPVGMISEILGENMAKGMEVEIAIRNHD
IPHVFPDAVLKQVSKLSEEVPEEAKSGRIDLRHLPLVTIDGEDARDFDDAVFCQKKRGGGWKLWVAIADVSYYVRLRTAL
DTEAHARGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCELNVSAKGKITGYEFYEAVMNSHARLTYTKVARILED
DEELCTRYQALVPHLKELHVMYQALVEARHQRGAIDFETIESKFVFNEMGRIERIEPVIRNDAHKIIEECMILANIAAAN
FMEKHQEPALFRIHAGPSEEKVTGFRQFLSECGLSLEGGSKPSTADYAKLLEQIRERPDHELLQTMLLRSLSQAVYHPDN
IGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKLKGSKRRTTDTGGYHYTLDDMDVLGDHCSMTERRADDATRDVAD
WLKCEYMQDHVGEEFTGVISSVTGFGLFVRLDDLFIDGLVHISTLDNDYYQFDLARQRLIGENSGMIYRIGDKVKIRVEA
VSLEQRQVDFSLVSSERKPLREGKTAKDKLKKNARYAESLEKQRHKRKPSTSKSAVRKTSVVKPSTTTKKASKKSAKKRK

Sequences:

>Translated_800_residues
MAKIKNNPHLQDPNYQEELAKYENPIPSREFILDTIRQHNAPISKEELLTTFAIQNEEQQEAMRRRLRAMENDGQLVFTK
RKRYALPEKLDLLKGTVLGHRDGYGFLQVEGKDSDWFIPNSQMQRVMHGDYVLAQPSGFDRRGRQEVRIVRVLQQRKKQI
VGRFFLEDGIGYVVPDDSRINRDILIPNEHRQGARMGQVVVVELKPRTATFSQPVGMISEILGENMAKGMEVEIAIRNHD
IPHVFPDAVLKQVSKLSEEVPEEAKSGRIDLRHLPLVTIDGEDARDFDDAVFCQKKRGGGWKLWVAIADVSYYVRLRTAL
DTEAHARGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCELNVSAKGKITGYEFYEAVMNSHARLTYTKVARILED
DEELCTRYQALVPHLKELHVMYQALVEARHQRGAIDFETIESKFVFNEMGRIERIEPVIRNDAHKIIEECMILANIAAAN
FMEKHQEPALFRIHAGPSEEKVTGFRQFLSECGLSLEGGSKPSTADYAKLLEQIRERPDHELLQTMLLRSLSQAVYHPDN
IGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKLKGSKRRTTDTGGYHYTLDDMDVLGDHCSMTERRADDATRDVAD
WLKCEYMQDHVGEEFTGVISSVTGFGLFVRLDDLFIDGLVHISTLDNDYYQFDLARQRLIGENSGMIYRIGDKVKIRVEA
VSLEQRQVDFSLVSSERKPLREGKTAKDKLKKNARYAESLEKQRHKRKPSTSKSAVRKTSVVKPSTTTKKASKKSAKKRK
>Mature_799_residues
AKIKNNPHLQDPNYQEELAKYENPIPSREFILDTIRQHNAPISKEELLTTFAIQNEEQQEAMRRRLRAMENDGQLVFTKR
KRYALPEKLDLLKGTVLGHRDGYGFLQVEGKDSDWFIPNSQMQRVMHGDYVLAQPSGFDRRGRQEVRIVRVLQQRKKQIV
GRFFLEDGIGYVVPDDSRINRDILIPNEHRQGARMGQVVVVELKPRTATFSQPVGMISEILGENMAKGMEVEIAIRNHDI
PHVFPDAVLKQVSKLSEEVPEEAKSGRIDLRHLPLVTIDGEDARDFDDAVFCQKKRGGGWKLWVAIADVSYYVRLRTALD
TEAHARGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLCMVCELNVSAKGKITGYEFYEAVMNSHARLTYTKVARILEDD
EELCTRYQALVPHLKELHVMYQALVEARHQRGAIDFETIESKFVFNEMGRIERIEPVIRNDAHKIIEECMILANIAAANF
MEKHQEPALFRIHAGPSEEKVTGFRQFLSECGLSLEGGSKPSTADYAKLLEQIRERPDHELLQTMLLRSLSQAVYHPDNI
GHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKLKGSKRRTTDTGGYHYTLDDMDVLGDHCSMTERRADDATRDVADW
LKCEYMQDHVGEEFTGVISSVTGFGLFVRLDDLFIDGLVHISTLDNDYYQFDLARQRLIGENSGMIYRIGDKVKIRVEAV
SLEQRQVDFSLVSSERKPLREGKTAKDKLKKNARYAESLEKQRHKRKPSTSKSAVRKTSVVKPSTTTKKASKKSAKKRK

Specific function: 3'-5'exoribonuclease that participates in an essential cell function. Acts nonspecifically on poly(A), poly(U) and ribosomal RNAs [H]

COG id: COG0557

COG function: function code K; Exoribonuclease R

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI190014623, Length=596, Percent_Identity=29.8657718120805, Blast_Score=188, Evalue=2e-47,
Organism=Homo sapiens, GI190014625, Length=593, Percent_Identity=30.0168634064081, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI134288890, Length=442, Percent_Identity=30.0904977375566, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI19115966, Length=592, Percent_Identity=26.8581081081081, Blast_Score=149, Evalue=9e-36,
Organism=Homo sapiens, GI219521928, Length=592, Percent_Identity=26.8581081081081, Blast_Score=149, Evalue=2e-35,
Organism=Escherichia coli, GI87082383, Length=738, Percent_Identity=66.260162601626, Blast_Score=1040, Evalue=0.0,
Organism=Escherichia coli, GI1787542, Length=653, Percent_Identity=27.2588055130168, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI212645896, Length=434, Percent_Identity=30.8755760368664, Blast_Score=177, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17553506, Length=418, Percent_Identity=31.3397129186603, Blast_Score=168, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6324552, Length=656, Percent_Identity=26.3719512195122, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI24649634, Length=467, Percent_Identity=30.8351177730193, Blast_Score=184, Evalue=3e-46,
Organism=Drosophila melanogaster, GI19922976, Length=373, Percent_Identity=28.4182305630027, Blast_Score=136, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24654597, Length=373, Percent_Identity=28.4182305630027, Blast_Score=136, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24654592, Length=373, Percent_Identity=28.4182305630027, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013223
- InterPro:   IPR001900
- InterPro:   IPR022966
- InterPro:   IPR004476
- InterPro:   IPR011805
- InterPro:   IPR013668 [H]

Pfam domain/function: PF08461 HTH_12; PF08206 OB_RNB; PF00773 RNB; PF00575 S1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 91524; Mature: 91393

Theoretical pI: Translated: 8.55; Mature: 8.55

Prosite motif: PS50126 S1 ; PS01175 RIBONUCLEASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKNNPHLQDPNYQEELAKYENPIPSREFILDTIRQHNAPISKEELLTTFAIQNEEQQ
CCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
EAMRRRLRAMENDGQLVFTKRKRYALPEKLDLLKGTVLGHRDGYGFLQVEGKDSDWFIPN
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCC
SQMQRVMHGDYVLAQPSGFDRRGRQEVRIVRVLQQRKKQIVGRFFLEDGIGYVVPDDSRI
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
NRDILIPNEHRQGARMGQVVVVELKPRTATFSQPVGMISEILGENMAKGMEVEIAIRNHD
CCCEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
IPHVFPDAVLKQVSKLSEEVPEEAKSGRIDLRHLPLVTIDGEDARDFDDAVFCQKKRGGG
CCCCCHHHHHHHHHHHHHHCCHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCC
WKLWVAIADVSYYVRLRTALDTEAHARGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLC
EEEEEEEECCEEEEHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHEE
MVCELNVSAKGKITGYEFYEAVMNSHARLTYTKVARILEDDEELCTRYQALVPHLKELHV
EEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MYQALVEARHQRGAIDFETIESKFVFNEMGRIERIEPVIRNDAHKIIEECMILANIAAAN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FMEKHQEPALFRIHAGPSEEKVTGFRQFLSECGLSLEGGSKPSTADYAKLLEQIRERPDH
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCH
ELLQTMLLRSLSQAVYHPDNIGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKLKGS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
KRRTTDTGGYHYTLDDMDVLGDHCSMTERRADDATRDVADWLKCEYMQDHVGEEFTGVIS
CCCCCCCCCEEEEECCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SVTGFGLFVRLDDLFIDGLVHISTLDNDYYQFDLARQRLIGENSGMIYRIGDKVKIRVEA
HHHHCEEEEEEHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEE
VSLEQRQVDFSLVSSERKPLREGKTAKDKLKKNARYAESLEKQRHKRKPSTSKSAVRKTS
ECHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VVKPSTTTKKASKKSAKKRK
CCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AKIKNNPHLQDPNYQEELAKYENPIPSREFILDTIRQHNAPISKEELLTTFAIQNEEQQ
CCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHH
EAMRRRLRAMENDGQLVFTKRKRYALPEKLDLLKGTVLGHRDGYGFLQVEGKDSDWFIPN
HHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHEECCCCCEEEEEECCCCCCCCCC
SQMQRVMHGDYVLAQPSGFDRRGRQEVRIVRVLQQRKKQIVGRFFLEDGIGYVVPDDSRI
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCC
NRDILIPNEHRQGARMGQVVVVELKPRTATFSQPVGMISEILGENMAKGMEVEIAIRNHD
CCCEECCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCC
IPHVFPDAVLKQVSKLSEEVPEEAKSGRIDLRHLPLVTIDGEDARDFDDAVFCQKKRGGG
CCCCCHHHHHHHHHHHHHHCCHHHHCCCEEEEECCEEEECCCCCCCCHHHHHHHHCCCCC
WKLWVAIADVSYYVRLRTALDTEAHARGNSVYFPNRVVPMLPEILSNGLCSLNPQVDRLC
EEEEEEEECCEEEEHHHHHHCCHHHCCCCCEECCCCHHHHHHHHHCCCCCCCCCCHHHEE
MVCELNVSAKGKITGYEFYEAVMNSHARLTYTKVARILEDDEELCTRYQALVPHLKELHV
EEEEECCCCCCCCCHHHHHHHHHCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
MYQALVEARHQRGAIDFETIESKFVFNEMGRIERIEPVIRNDAHKIIEECMILANIAAAN
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
FMEKHQEPALFRIHAGPSEEKVTGFRQFLSECGLSLEGGSKPSTADYAKLLEQIRERPDH
HHHHCCCCCEEEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHCCCH
ELLQTMLLRSLSQAVYHPDNIGHFGLALEEYAHFTSPIRRYPDLTLHRGIKYLLAKLKGS
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
KRRTTDTGGYHYTLDDMDVLGDHCSMTERRADDATRDVADWLKCEYMQDHVGEEFTGVIS
CCCCCCCCCEEEEECCHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SVTGFGLFVRLDDLFIDGLVHISTLDNDYYQFDLARQRLIGENSGMIYRIGDKVKIRVEA
HHHHCEEEEEEHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCEEEEEEEE
VSLEQRQVDFSLVSSERKPLREGKTAKDKLKKNARYAESLEKQRHKRKPSTSKSAVRKTS
ECHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
VVKPSTTTKKASKKSAKKRK
CCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]