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The map label for this gene is rlmB

Identifier: 15603818

GI number: 15603818

Start: 2190954

End: 2191691

Strand: Reverse

Name: rlmB

Synonym: PM1953

Alternate gene names: 15603818

Gene position: 2191691-2190954 (Counterclockwise)

Preceding gene: 15603819

Following gene: 15603815

Centisome position: 97.09

GC content: 44.99

Gene sequence:

>738_bases
ATGAGTGAAAATATTTATGGCATTCATGCAGTAAGTGCCTTTTTAAACAACGCGCCAGAGCGTTTAATTGAAGTTTATGC
ACTCAAAGGGCGCGACGATAAACGTTTACAACCTTTGCTCAATGAACTTCATCGGTTGGGTATTACTATCCAATTTGTTA
ATCGCCAAACCCTTGATAAAAAAGCAGAGGGCGAAGTACACCAAGGTATCATGGCACGTGTTCAGCCAGCGAAAGAATTG
AATGAAGCAGATTTAGATACGCTTTTACAAAATCAGTCCAATCCGTTGCTATTAGTGTTAGATGGTGTGACTGATCCACA
TAACTTAGGTGCTTGTTTGCGTACTGCGGATGCAGCGGGCGTGTGTGCTGTGATAGTACCAAAAGATAAATCTGCCCAAT
TAACCGCTATCGCACGTAAAGTAGCTTGTGGTGCCGCTGAAGTCGTGCCATTAATCCGTGTAACCAACCTCGCGCGCACT
TTGCGTGAATTACAGCAGAAGCACAACATTTGGGTGGTTGGTACTGCAGGTGAAGCCACAAATACGCTCTATCAAACGCA
ATTAACGGGGGGCTTAGCCCTTGTAATGGGAGCGGAAGGTGAAGGGATGCGTCGTTTAACGCGTGAGCATTGTGATCAAC
TGATTAGTATTCCAATGGCGGGTTCAGTCTCTTCGCTGAATGTTTCTGTGGCGACAGGGGTTTGCTTATTTGAAATTGTC
AGACAGCGTTTAGCCTAA

Upstream 100 bases:

>100_bases
ACCAAGCACCAGTAAAAGTGCGGTAAGAAAAACAAGCGTTGTTAAACCATCAACAACCACGAAAAAAGCCAGTAAGAAAT
CAGCTAAAAAGAGAAAGTAG

Downstream 100 bases:

>100_bases
ACTGAGAGTAAAACTGAGCGCACTTTATTGCCATAAAAAAGCGTAATGAAAAATTTCATTACGCTTTTTTGTTCATCAAT
AGGCTTTATTTGATCACACC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSENIYGIHAVSAFLNNAPERLIEVYALKGRDDKRLQPLLNELHRLGITIQFVNRQTLDKKAEGEVHQGIMARVQPAKEL
NEADLDTLLQNQSNPLLLVLDGVTDPHNLGACLRTADAAGVCAVIVPKDKSAQLTAIARKVACGAAEVVPLIRVTNLART
LRELQQKHNIWVVGTAGEATNTLYQTQLTGGLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLA

Sequences:

>Translated_245_residues
MSENIYGIHAVSAFLNNAPERLIEVYALKGRDDKRLQPLLNELHRLGITIQFVNRQTLDKKAEGEVHQGIMARVQPAKEL
NEADLDTLLQNQSNPLLLVLDGVTDPHNLGACLRTADAAGVCAVIVPKDKSAQLTAIARKVACGAAEVVPLIRVTNLART
LRELQQKHNIWVVGTAGEATNTLYQTQLTGGLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLA
>Mature_244_residues
SENIYGIHAVSAFLNNAPERLIEVYALKGRDDKRLQPLLNELHRLGITIQFVNRQTLDKKAEGEVHQGIMARVQPAKELN
EADLDTLLQNQSNPLLLVLDGVTDPHNLGACLRTADAAGVCAVIVPKDKSAQLTAIARKVACGAAEVVPLIRVTNLARTL
RELQQKHNIWVVGTAGEATNTLYQTQLTGGLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIVR
QRLA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=217, Percent_Identity=32.258064516129, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=72.8395061728395, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI1788935, Length=242, Percent_Identity=29.3388429752066, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17555908, Length=258, Percent_Identity=27.5193798449612, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324775, Length=290, Percent_Identity=26.551724137931, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_PASMU (Q9CJP3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246892.1
- ProteinModelPortal:   Q9CJP3
- SMR:   Q9CJP3
- GeneID:   1245300
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1953
- NMPDR:   fig|272843.1.peg.1954
- HOGENOM:   HBG714057
- OMA:   NELVILM
- ProtClustDB:   PRK11181
- BioCyc:   PMUL272843:PM1953-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26557; Mature: 26426

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: BINDING 197-197 BINDING 217-217 BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENIYGIHAVSAFLNNAPERLIEVYALKGRDDKRLQPLLNELHRLGITIQFVNRQTLDK
CCCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCHHCCH
KAEGEVHQGIMARVQPAKELNEADLDTLLQNQSNPLLLVLDGVTDPHNLGACLRTADAAG
HHCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VCAVIVPKDKSAQLTAIARKVACGAAEVVPLIRVTNLARTLRELQQKHNIWVVGTAGEAT
EEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
NTLYQTQLTGGLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLA
HHHCC
>Mature Secondary Structure 
SENIYGIHAVSAFLNNAPERLIEVYALKGRDDKRLQPLLNELHRLGITIQFVNRQTLDK
CCCEEHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCEEEEEECCHHCCH
KAEGEVHQGIMARVQPAKELNEADLDTLLQNQSNPLLLVLDGVTDPHNLGACLRTADAAG
HHCCHHHHHHHHHCCCHHHCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCC
VCAVIVPKDKSAQLTAIARKVACGAAEVVPLIRVTNLARTLRELQQKHNIWVVGTAGEAT
EEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCHH
NTLYQTQLTGGLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLA
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100