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The map label for this gene is afuB_2 [H]

Identifier: 15603320

GI number: 15603320

Start: 1644341

End: 1646035

Strand: Reverse

Name: afuB_2 [H]

Synonym: PM1455

Alternate gene names: 15603320

Gene position: 1646035-1644341 (Counterclockwise)

Preceding gene: 15603321

Following gene: 15603319

Centisome position: 72.91

GC content: 41.71

Gene sequence:

>1695_bases
ATGGCCGCAGTGATTCAAAAAAGTCCATTTAATGTGGCGAATGCGATTTTAGCGTTATCAATTGGGATTTTGGTGATTGT
GGTTGCTGTCCCGGTGTTATTGATTTTTATTAATTCCTTTTGGGTAAATGGGCAATTTAATGTTACCGATGTCGTCAATA
TTTTGAAACAGAAAGAAACCTACGAAGCCTTATTGAACTCATTATTCATTGCCGTTGGTGTGACGATCATGAGTACGATC
GTGGGGACATTTTTTGCGTGGTTAGTGACACGTACCGATTTGCCTTATAAAGGGCTGATGAAAGTGATGTTTCTCGTGCC
TTTTATGTTGCCGTCTTTTATTGGGGCGTTAGCTTGGAAAATGTTACTTTCCCCTCGTGCCGGCTATATTAACCGTTTCT
TTATGGATACGTTTGGTTTAGAGGAACCGATTTTCAATATCTACAGTTATACGGGCATTATGGCGGTAGAAACCATGTAT
TTGTTCCCCTTCGTCTTTATTCAAGTATGTGGTGCACTTGAGCGTATGGACCCGACGTTAGAAGAATCAGCGCGTATTTC
TGGCGCCAGTCTTTTTACCATTACACGTAAAATCACGATTCCATTAGTGATGCCGAGTATCGTGTCGGGTGCGTTATTGA
TTATGCTTTACTCTATGGCTCACTTTGGTACGGTGGCTGTATTAGGGGTGGAAAATGGGATTTTCAATATTCCGACCTTA
ATTTATGAACGTATCCATCAAAGTGCGGGGAGTTTTGAGGCAATTCGGACCGGAACGGTGTTAGCGACAGTATTAGTGGT
GACAGCTGCGTTGATTATTTGGTTGCAAAATAAAATTCTTAATCGTGGACGTTTCCAAATTATTGCGGGTAAAAGTTTCC
GTCCTGTTGAGGTGAAACTCAGAGGATTAAGAATGCCATTATTGTTTATTTGTGTGTTCTATATCGCGTTTACCATTGTG
TTACCAACAGTCACGATTTTTATCGTGGGAGGGTTAAAAACCTATGGTCTGCCAATTACTTGGGAAAACCTCACCTTTGA
TAACTACAAATTTATCTTGTTTGACTGGCAGCTCACTAAAGATGCGATCCGCAATAGTGTGCTATTAGGTTTAGCCGCGG
CGGTGATAACGATGTTTGCTGGGGTAATGATCTCTTATGTCATTGTGAAGATGCGAGTGAAAGGCAAAGGGATTCTTGAA
TTCTTGGGTATGTTGCCATTCTCAGTACCTGGCTCGGTGATTGCGTTAGGGGTTATTTTAGCGTGGAGCGGTAAATTTGG
TATTAATTTATACAACACGGTATGGATTATTTTGATTGCCTATATTGCGCGTTACATGGCGTTCTCACTAAAAGCCAACT
CCGCAGCGCTAGAACAAGTACATGACTCTTTAGTCGAAGCAGCACGTGCTTGTGGTGCAAGTATGTGGCAATCGCTGAAA
GATATTGTCGTGCCGTTAGTGCGTCCGGGAATGATTGCTGCTTTCTTCCTCATTTTCTTACCAGCATTAAGAGAATTAAC
GGTTTCGGTAATGCTATATGGTCCGTCAACACGTACGATCGGGGTAGCGATTTATACCCTGAATGAAGATGGCGAAACAG
TTTATTCTGCAGCATTAGCGGGTATTGCATTAATTATTATTGTGTTTGGGCAAACCGTGATTAAACGTTATGCCGAGAAG
AAAACACAGAAATAG

Upstream 100 bases:

>100_bases
ATTTGATGTTCCAAGAGGGGGAAGACTGTTACGTTAATTTCCATGATTTGCTTTGGTTTACTGATAATCAACAACATGTT
CAGCAAACAAGGGGGTAGCA

Downstream 100 bases:

>100_bases
CCGAATATTAAGGAGAAAATGATGAGCTATATTCAACTTAATCATGTGATGAAAAAATTCGGCGATGTCATTGCCATTCC
TGATCTGAGCCTCAGTATTG

Product: AfuB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MAAVIQKSPFNVANAILALSIGILVIVVAVPVLLIFINSFWVNGQFNVTDVVNILKQKETYEALLNSLFIAVGVTIMSTI
VGTFFAWLVTRTDLPYKGLMKVMFLVPFMLPSFIGALAWKMLLSPRAGYINRFFMDTFGLEEPIFNIYSYTGIMAVETMY
LFPFVFIQVCGALERMDPTLEESARISGASLFTITRKITIPLVMPSIVSGALLIMLYSMAHFGTVAVLGVENGIFNIPTL
IYERIHQSAGSFEAIRTGTVLATVLVVTAALIIWLQNKILNRGRFQIIAGKSFRPVEVKLRGLRMPLLFICVFYIAFTIV
LPTVTIFIVGGLKTYGLPITWENLTFDNYKFILFDWQLTKDAIRNSVLLGLAAAVITMFAGVMISYVIVKMRVKGKGILE
FLGMLPFSVPGSVIALGVILAWSGKFGINLYNTVWIILIAYIARYMAFSLKANSAALEQVHDSLVEAARACGASMWQSLK
DIVVPLVRPGMIAAFFLIFLPALRELTVSVMLYGPSTRTIGVAIYTLNEDGETVYSAALAGIALIIIVFGQTVIKRYAEK
KTQK

Sequences:

>Translated_564_residues
MAAVIQKSPFNVANAILALSIGILVIVVAVPVLLIFINSFWVNGQFNVTDVVNILKQKETYEALLNSLFIAVGVTIMSTI
VGTFFAWLVTRTDLPYKGLMKVMFLVPFMLPSFIGALAWKMLLSPRAGYINRFFMDTFGLEEPIFNIYSYTGIMAVETMY
LFPFVFIQVCGALERMDPTLEESARISGASLFTITRKITIPLVMPSIVSGALLIMLYSMAHFGTVAVLGVENGIFNIPTL
IYERIHQSAGSFEAIRTGTVLATVLVVTAALIIWLQNKILNRGRFQIIAGKSFRPVEVKLRGLRMPLLFICVFYIAFTIV
LPTVTIFIVGGLKTYGLPITWENLTFDNYKFILFDWQLTKDAIRNSVLLGLAAAVITMFAGVMISYVIVKMRVKGKGILE
FLGMLPFSVPGSVIALGVILAWSGKFGINLYNTVWIILIAYIARYMAFSLKANSAALEQVHDSLVEAARACGASMWQSLK
DIVVPLVRPGMIAAFFLIFLPALRELTVSVMLYGPSTRTIGVAIYTLNEDGETVYSAALAGIALIIIVFGQTVIKRYAEK
KTQK
>Mature_563_residues
AAVIQKSPFNVANAILALSIGILVIVVAVPVLLIFINSFWVNGQFNVTDVVNILKQKETYEALLNSLFIAVGVTIMSTIV
GTFFAWLVTRTDLPYKGLMKVMFLVPFMLPSFIGALAWKMLLSPRAGYINRFFMDTFGLEEPIFNIYSYTGIMAVETMYL
FPFVFIQVCGALERMDPTLEESARISGASLFTITRKITIPLVMPSIVSGALLIMLYSMAHFGTVAVLGVENGIFNIPTLI
YERIHQSAGSFEAIRTGTVLATVLVVTAALIIWLQNKILNRGRFQIIAGKSFRPVEVKLRGLRMPLLFICVFYIAFTIVL
PTVTIFIVGGLKTYGLPITWENLTFDNYKFILFDWQLTKDAIRNSVLLGLAAAVITMFAGVMISYVIVKMRVKGKGILEF
LGMLPFSVPGSVIALGVILAWSGKFGINLYNTVWIILIAYIARYMAFSLKANSAALEQVHDSLVEAARACGASMWQSLKD
IVVPLVRPGMIAAFFLIFLPALRELTVSVMLYGPSTRTIGVAIYTLNEDGETVYSAALAGIALIIIVFGQTVIKRYAEKK
TQK

Specific function: Part of the ABC transporter complex fbpABC (TC 3.A.1.10.1) involved in Fe(3+) ions import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=268, Percent_Identity=26.865671641791, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082099, Length=293, Percent_Identity=23.2081911262799, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1786980, Length=206, Percent_Identity=29.6116504854369, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788764, Length=168, Percent_Identity=29.1666666666667, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 62269; Mature: 62137

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAVIQKSPFNVANAILALSIGILVIVVAVPVLLIFINSFWVNGQFNVTDVVNILKQKET
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
YEALLNSLFIAVGVTIMSTIVGTFFAWLVTRTDLPYKGLMKVMFLVPFMLPSFIGALAWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSPRAGYINRFFMDTFGLEEPIFNIYSYTGIMAVETMYLFPFVFIQVCGALERMDPTL
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EESARISGASLFTITRKITIPLVMPSIVSGALLIMLYSMAHFGTVAVLGVENGIFNIPTL
HHHHCCCCCEEEEEEEHEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
IYERIHQSAGSFEAIRTGTVLATVLVVTAALIIWLQNKILNRGRFQIIAGKSFRPVEVKL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RGLRMPLLFICVFYIAFTIVLPTVTIFIVGGLKTYGLPITWENLTFDNYKFILFDWQLTK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEECCCEECCEEEEEEEEEHHH
DAIRNSVLLGLAAAVITMFAGVMISYVIVKMRVKGKGILEFLGMLPFSVPGSVIALGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AWSGKFGINLYNTVWIILIAYIARYMAFSLKANSAALEQVHDSLVEAARACGASMWQSLK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVVPLVRPGMIAAFFLIFLPALRELTVSVMLYGPSTRTIGVAIYTLNEDGETVYSAALA
HHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHH
GIALIIIVFGQTVIKRYAEKKTQK
HHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AAVIQKSPFNVANAILALSIGILVIVVAVPVLLIFINSFWVNGQFNVTDVVNILKQKET
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHHH
YEALLNSLFIAVGVTIMSTIVGTFFAWLVTRTDLPYKGLMKVMFLVPFMLPSFIGALAWK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MLLSPRAGYINRFFMDTFGLEEPIFNIYSYTGIMAVETMYLFPFVFIQVCGALERMDPTL
HHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EESARISGASLFTITRKITIPLVMPSIVSGALLIMLYSMAHFGTVAVLGVENGIFNIPTL
HHHHCCCCCEEEEEEEHEEEHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHH
IYERIHQSAGSFEAIRTGTVLATVLVVTAALIIWLQNKILNRGRFQIIAGKSFRPVEVKL
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHH
RGLRMPLLFICVFYIAFTIVLPTVTIFIVGGLKTYGLPITWENLTFDNYKFILFDWQLTK
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEECCCEECCEEEEEEEEEHHH
DAIRNSVLLGLAAAVITMFAGVMISYVIVKMRVKGKGILEFLGMLPFSVPGSVIALGVIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHH
AWSGKFGINLYNTVWIILIAYIARYMAFSLKANSAALEQVHDSLVEAARACGASMWQSLK
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIVVPLVRPGMIAAFFLIFLPALRELTVSVMLYGPSTRTIGVAIYTLNEDGETVYSAALA
HHHHHHCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEEEEEEECCCCHHHHHHHHH
GIALIIIVFGQTVIKRYAEKKTQK
HHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8807793 [H]