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The map label for this gene is greB

Identifier: 15603315

GI number: 15603315

Start: 1638200

End: 1638676

Strand: Direct

Name: greB

Synonym: PM1450

Alternate gene names: 15603315

Gene position: 1638200-1638676 (Clockwise)

Preceding gene: 15603311

Following gene: 15603318

Centisome position: 72.57

GC content: 44.65

Gene sequence:

>477_bases
ATGGCAAAATCAAATTACATCACACGTTCTGGCTGGAATGCCTTAGATCAAGAACTCAAATTCTTATGGAAAGATGAACG
TCCGAAAGTGACCCAAGCGGTGTCAGATGCCGCGGCGTTAGGCGATCGTAGTGAAAATGCGGAATATATTTATGGCAAAC
GCCGTTTACGCGAAATCGACCGTCGTGTGCGTTTTTTAACCAAACGCTTAGAAGTCTTGCAAATCGTCGATTATCACCCT
AAACAAGAAGGCAAAGTCTTTTTTGGTGCGTGGGTCGCCCTCGAAAATGAAGCGGGAGAAGAAAAACACTACCGTATCGT
CGGCTGTGATGAATTTGATCCCGCGAAAAATTGGATCTCGATTGACTCACCGGTGGCGCGTGCCTTAATTGGCAAATCTA
TTGATGATGAGATTAAAGTCGAGACGCCCTCCGGCTTGGTGATACTCTATATCAACCAAATTTGGTACGAAAAATAA

Upstream 100 bases:

>100_bases
CATTATACAGAAAAGCGGAAAATAAAAAATAGCCTGCGCGCTTACCCCAAAGGCATCCAATGCGGTAGAATAATCCTATC
TTTTTAGATGGGTGGACAGT

Downstream 100 bases:

>100_bases
AAGAGGCAGAAATCTGCCTCTTTTTTCACTTACTTATTTCTCTTTAGCTTGTGCAAATTTCTGACTAAAGAAATGTGTAT
GTAACGCAATAGAATCTTTC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAKSNYITRSGWNALDQELKFLWKDERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYHP
KQEGKVFFGAWVALENEAGEEKHYRIVGCDEFDPAKNWISIDSPVARALIGKSIDDEIKVETPSGLVILYINQIWYEK

Sequences:

>Translated_158_residues
MAKSNYITRSGWNALDQELKFLWKDERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYHP
KQEGKVFFGAWVALENEAGEEKHYRIVGCDEFDPAKNWISIDSPVARALIGKSIDDEIKVETPSGLVILYINQIWYEK
>Mature_157_residues
AKSNYITRSGWNALDQELKFLWKDERPKVTQAVSDAAALGDRSENAEYIYGKRRLREIDRRVRFLTKRLEVLQIVDYHPK
QEGKVFFGAWVALENEAGEEKHYRIVGCDEFDPAKNWISIDSPVARALIGKSIDDEIKVETPSGLVILYINQIWYEK

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=156, Percent_Identity=58.974358974359, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=37.5, Blast_Score=98, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_PASMU (P57802)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246389.1
- ProteinModelPortal:   P57802
- SMR:   P57802
- GeneID:   1244797
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1450
- NMPDR:   fig|272843.1.peg.1451
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- BioCyc:   PMUL272843:PM1450-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18348; Mature: 18217

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSNYITRSGWNALDQELKFLWKDERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYHPKQEGKVFFGAWVALENEAGEEKHYRIVGCDEFDPAKNWIS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEE
IDSPVARALIGKSIDDEIKVETPSGLVILYINQIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEECHHCCC
>Mature Secondary Structure 
AKSNYITRSGWNALDQELKFLWKDERPKVTQAVSDAAALGDRSENAEYIYGKRRLREID
CCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
RRVRFLTKRLEVLQIVDYHPKQEGKVFFGAWVALENEAGEEKHYRIVGCDEFDPAKNWIS
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEECCCCCCCCEEEECCCCCCCCCCEEE
IDSPVARALIGKSIDDEIKVETPSGLVILYINQIWYEK
CCCHHHHHHHCCCCCCCEEEECCCCEEEEEEECHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100