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The map label for this gene is glpK

Identifier: 15603311

GI number: 15603311

Start: 1633236

End: 1634744

Strand: Direct

Name: glpK

Synonym: PM1446

Alternate gene names: 15603311

Gene position: 1633236-1634744 (Clockwise)

Preceding gene: 15603310

Following gene: 15603315

Centisome position: 72.35

GC content: 46.12

Gene sequence:

>1509_bases
ATGACGGATAAAAAATATATCATTGCCCTAGATCAAGGTACAACAAGTTCAAGAGCGGTTCTTCTTGATCACGATGCCAA
TATTGTTGAAATTGCCCAACGTGAATTTACGCAAATTTATCCACAGGCTGGCTGGGTTGAACATAATCCCATGGAGATTT
GGGCAACACAAAGTTCCACCTTAAATGAAGTGGTCGCGAAAGCCGGTATCACTGCTGATCAAATCGCGGCAATTGGGATC
ACTAATCAGCGTGAAACCACGATTGTATGGGAAAAAGAAACCGGTAAGCCAATTTACAATGCGATTGTGTGGCAATGCCG
GCGTACGACTGAGATAACCGATAAACTGAAAGCAGATGGTCACGAAGACTATATACGTCAAACGACAGGCTTAGTGGTAG
ATCCTTACTTCTCTGGAACCAAAATTAAATGGATTTTGGATAACGTGGAAGGGGCACGGGAACAAGCAGAACGTGGCGAA
TTACTCTTTGGTACGGTGGATACTTGGCTAGTATGGAAATTAACTCAAGGGCGTGCACATGTCACCGATTACACTAACGC
CTCGCGGACGATGTTATTTAATATTCATACTCTGCAATGGGACGATAACATGCTGGCGATTTTAGGTATCCCACGTGCTA
TGTTGCCTGAGGTGCGTAATTCTTCTGAAATATATGGTCAAACCAATATCGGTGGCAAAGGTGGCGTACGTATTCCTGTA
GCCGGTATGGCAGGAGATCAACAAGCGGCCTTATATGGTCATCTTTGTGTTAATGCGGGACAAGCGAAAAACACTTATGG
TACCGGTTGTTTCATGTTAATGCATACGGGCGACAACGCCGTACAATCACAAAATGGCTTATTAACCACCATTGCCTGTA
ATGCCAAAGGTGAGCCTGCATATGCTCTCGAAGGCTCCATCTTCATGGGCGGGGCATCCATTCAATGGCTACGCGATGAA
CTCAAAATTGTGCATGATAGCTATGACTCAGAATATTTTGCTACCCGAGTACCTGATTGTAATGGTGTCTATGTGGTACC
TGCCTTTACTGGATTAGGTGCCCCTTATTGGGATCCCTATGCACGCGGTGCCATTTTTGGGCTCTCACGTGGTGCAAATC
GCAACCACATTGTGCGCGCTACCTTAGAATCCATTGCATACCAAACTCGCGATGTGCTAGAGGCGATGCAATCTGACTCA
GGACAAACACTGCAAGCGCTCCGCGTGGACGGTGGAGCAACGGAAAATAATTTCTTAATGCAGTTCCAAGCGGATATCCT
CGCCACCAAAGTAGAAAGACCAAAAGTGAAAGAAGTCACGGCATTAGGCGCCGCCTATCTTGCAGGTCTTGCGACGGGTT
TCTGGAAAGATCTCAGTGAATTGCGTGATAAAGCCAGCATCGAGAAAACTTTTGTTCCCGATGGAGATGAAGCCAAACGC
ACTCGCCGTTATAAAGGCTGGAAAAAAGCAGTCAAACGTGCGCTCGAATGGGAAAAAGAGGAAGAGTAG

Upstream 100 bases:

>100_bases
GGTGGGCTTTTTGGTGGTTGGATTTATACCAAATTCGTCGGCAAAAACTTACCTTGTCATTGCGAATAATTCACGTTCAT
TTAAACAGAGGAAAAACATG

Downstream 100 bases:

>100_bases
TACGCCTTAATCGTGAAAAAGTGCGGTCGATATTTTAACAAAATTGACCGCACTTTGTTTATATGGGGTATTAATCCACC
AACTCAAACGCAATCACCCA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTDKKYIIALDQGTTSSRAVLLDHDANIVEIAQREFTQIYPQAGWVEHNPMEIWATQSSTLNEVVAKAGITADQIAAIGI
TNQRETTIVWEKETGKPIYNAIVWQCRRTTEITDKLKADGHEDYIRQTTGLVVDPYFSGTKIKWILDNVEGAREQAERGE
LLFGTVDTWLVWKLTQGRAHVTDYTNASRTMLFNIHTLQWDDNMLAILGIPRAMLPEVRNSSEIYGQTNIGGKGGVRIPV
AGMAGDQQAALYGHLCVNAGQAKNTYGTGCFMLMHTGDNAVQSQNGLLTTIACNAKGEPAYALEGSIFMGGASIQWLRDE
LKIVHDSYDSEYFATRVPDCNGVYVVPAFTGLGAPYWDPYARGAIFGLSRGANRNHIVRATLESIAYQTRDVLEAMQSDS
GQTLQALRVDGGATENNFLMQFQADILATKVERPKVKEVTALGAAYLAGLATGFWKDLSELRDKASIEKTFVPDGDEAKR
TRRYKGWKKAVKRALEWEKEEE

Sequences:

>Translated_502_residues
MTDKKYIIALDQGTTSSRAVLLDHDANIVEIAQREFTQIYPQAGWVEHNPMEIWATQSSTLNEVVAKAGITADQIAAIGI
TNQRETTIVWEKETGKPIYNAIVWQCRRTTEITDKLKADGHEDYIRQTTGLVVDPYFSGTKIKWILDNVEGAREQAERGE
LLFGTVDTWLVWKLTQGRAHVTDYTNASRTMLFNIHTLQWDDNMLAILGIPRAMLPEVRNSSEIYGQTNIGGKGGVRIPV
AGMAGDQQAALYGHLCVNAGQAKNTYGTGCFMLMHTGDNAVQSQNGLLTTIACNAKGEPAYALEGSIFMGGASIQWLRDE
LKIVHDSYDSEYFATRVPDCNGVYVVPAFTGLGAPYWDPYARGAIFGLSRGANRNHIVRATLESIAYQTRDVLEAMQSDS
GQTLQALRVDGGATENNFLMQFQADILATKVERPKVKEVTALGAAYLAGLATGFWKDLSELRDKASIEKTFVPDGDEAKR
TRRYKGWKKAVKRALEWEKEEE
>Mature_501_residues
TDKKYIIALDQGTTSSRAVLLDHDANIVEIAQREFTQIYPQAGWVEHNPMEIWATQSSTLNEVVAKAGITADQIAAIGIT
NQRETTIVWEKETGKPIYNAIVWQCRRTTEITDKLKADGHEDYIRQTTGLVVDPYFSGTKIKWILDNVEGAREQAERGEL
LFGTVDTWLVWKLTQGRAHVTDYTNASRTMLFNIHTLQWDDNMLAILGIPRAMLPEVRNSSEIYGQTNIGGKGGVRIPVA
GMAGDQQAALYGHLCVNAGQAKNTYGTGCFMLMHTGDNAVQSQNGLLTTIACNAKGEPAYALEGSIFMGGASIQWLRDEL
KIVHDSYDSEYFATRVPDCNGVYVVPAFTGLGAPYWDPYARGAIFGLSRGANRNHIVRATLESIAYQTRDVLEAMQSDSG
QTLQALRVDGGATENNFLMQFQADILATKVERPKVKEVTALGAAYLAGLATGFWKDLSELRDKASIEKTFVPDGDEAKRT
RRYKGWKKAVKRALEWEKEEE

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=45.2191235059761, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=45.2191235059761, Blast_Score=427, Evalue=1e-119,
Organism=Homo sapiens, GI42794763, Length=506, Percent_Identity=44.8616600790514, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.4223107569721, Blast_Score=416, Evalue=1e-116,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=33.2677165354331, Blast_Score=315, Evalue=6e-86,
Organism=Escherichia coli, GI1790361, Length=502, Percent_Identity=77.6892430278884, Blast_Score=841, Evalue=0.0,
Organism=Escherichia coli, GI1787789, Length=501, Percent_Identity=25.1497005988024, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1789987, Length=507, Percent_Identity=24.0631163708087, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790005, Length=491, Percent_Identity=23.8289205702648, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1789138, Length=507, Percent_Identity=22.4852071005917, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789168, Length=494, Percent_Identity=21.0526315789474, Blast_Score=75, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17535599, Length=502, Percent_Identity=42.6294820717131, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71986140, Length=524, Percent_Identity=25.381679389313, Blast_Score=174, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=301, Percent_Identity=30.8970099667774, Blast_Score=138, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6321755, Length=559, Percent_Identity=37.2093023255814, Blast_Score=332, Evalue=6e-92,
Organism=Drosophila melanogaster, GI17864214, Length=511, Percent_Identity=43.2485322896282, Blast_Score=414, Evalue=1e-116,
Organism=Drosophila melanogaster, GI24655555, Length=500, Percent_Identity=41.8, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655560, Length=500, Percent_Identity=41.8, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655570, Length=500, Percent_Identity=41.8, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655574, Length=500, Percent_Identity=41.8, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655565, Length=500, Percent_Identity=41.8, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24654573, Length=457, Percent_Identity=44.4201312910284, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI19922058, Length=521, Percent_Identity=35.5086372360845, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24652871, Length=500, Percent_Identity=35.6, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=28.7937743190661, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI21357867, Length=529, Percent_Identity=28.3553875236295, Blast_Score=228, Evalue=7e-60,
Organism=Drosophila melanogaster, GI24656372, Length=361, Percent_Identity=30.4709141274238, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI45552909, Length=299, Percent_Identity=28.7625418060201, Blast_Score=129, Evalue=6e-30,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_PASMU (P57944)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246385.1
- ProteinModelPortal:   P57944
- SMR:   P57944
- GeneID:   1244793
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1446
- NMPDR:   fig|272843.1.peg.1447
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- ProtClustDB:   PRK00047
- BioCyc:   PMUL272843:PM1446-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 55661; Mature: 55530

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 14-14 BINDING 18-18 BINDING 84-84 BINDING 136-136 BINDING 246-246 BINDING 268-268 BINDING 311-311

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKKYIIALDQGTTSSRAVLLDHDANIVEIAQREFTQIYPQAGWVEHNPMEIWATQSST
CCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LNEVVAKAGITADQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTTEITDKLKADG
HHHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCC
HEDYIRQTTGLVVDPYFSGTKIKWILDNVEGAREQAERGELLFGTVDTWLVWKLTQGRAH
HHHHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEECCEEEEEEEECCCEE
VTDYTNASRTMLFNIHTLQWDDNMLAILGIPRAMLPEVRNSSEIYGQTNIGGKGGVRIPV
EEECCCCCEEEEEEEEEEEECCCEEEEECCCHHHHHHHCCCCCEEEECCCCCCCCEEEEE
AGMAGDQQAALYGHLCVNAGQAKNTYGTGCFMLMHTGDNAVQSQNGLLTTIACNAKGEPA
ECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHCCCCEEEEEEECCCCCCC
YALEGSIFMGGASIQWLRDELKIVHDSYDSEYFATRVPDCNGVYVVPAFTGLGAPYWDPY
EEECCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHH
ARGAIFGLSRGANRNHIVRATLESIAYQTRDVLEAMQSDSGQTLQALRVDGGATENNFLM
HCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
QFQADILATKVERPKVKEVTALGAAYLAGLATGFWKDLSELRDKASIEKTFVPDGDEAKR
EEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
TRRYKGWKKAVKRALEWEKEEE
HHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDKKYIIALDQGTTSSRAVLLDHDANIVEIAQREFTQIYPQAGWVEHNPMEIWATQSST
CCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LNEVVAKAGITADQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRRTTEITDKLKADG
HHHHHHHCCCCHHHEEEEECCCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHHHCCC
HEDYIRQTTGLVVDPYFSGTKIKWILDNVEGAREQAERGELLFGTVDTWLVWKLTQGRAH
HHHHHHHHCCCEEECCCCCCEEEEEEECCHHHHHHHHCCCEEEEECCEEEEEEEECCCEE
VTDYTNASRTMLFNIHTLQWDDNMLAILGIPRAMLPEVRNSSEIYGQTNIGGKGGVRIPV
EEECCCCCEEEEEEEEEEEECCCEEEEECCCHHHHHHHCCCCCEEEECCCCCCCCEEEEE
AGMAGDQQAALYGHLCVNAGQAKNTYGTGCFMLMHTGDNAVQSQNGLLTTIACNAKGEPA
ECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEECCCCHHHCCCCEEEEEEECCCCCCC
YALEGSIFMGGASIQWLRDELKIVHDSYDSEYFATRVPDCNGVYVVPAFTGLGAPYWDPY
EEECCCEEECCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHH
ARGAIFGLSRGANRNHIVRATLESIAYQTRDVLEAMQSDSGQTLQALRVDGGATENNFLM
HCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEE
QFQADILATKVERPKVKEVTALGAAYLAGLATGFWKDLSELRDKASIEKTFVPDGDEAKR
EEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHH
TRRYKGWKKAVKRALEWEKEEE
HHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100