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The map label for this gene is glpF [H]

Identifier: 15603310

GI number: 15603310

Start: 1632416

End: 1633204

Strand: Direct

Name: glpF [H]

Synonym: PM1445

Alternate gene names: 15603310

Gene position: 1632416-1633204 (Clockwise)

Preceding gene: 15603309

Following gene: 15603311

Centisome position: 72.31

GC content: 48.04

Gene sequence:

>789_bases
ATGGATAAATCATTAAAAGCCGCCTGTATTGGCGAATTCTTAGGTACCGCATTACTTATCTTCTTTGGTGTAGGTTGTGT
CGCGGCATTAAAAGTCGCGGGAGCAAACTTTGGACAGTGGGAAATCAGTATCATGTGGGGGTTAGGGGTCGCAATCGCGG
TTTACGCTTCCGCCGGTCTTTCTGGTGCACATCTCAATCCCGCCGTCACGATCGCGTTATGGCAGTTTGCCTGTTTTGAC
GGTAAAAAAGTCGTTCCTTACATCTTAGCACAACTTGCAGGCGCCTTCTGTTCCGCTGCTTTAGTGTACTTTTTATACCA
TAACCTTTTCATTGATGTTGAAATCGCCCAGCAAATGGTACGTGGTACGCCGGAGAGTCTTTCACTCGCCAGCACCTTCT
CAACCTACCCCCATCCAAGTATTAGCGTGTTACACGCCTTTATCGTGGAATTTGTGATTACGGCTGTACTCATGGCATTA
ATTCTAGCCCTCACGGATGATGGTAATGGGTTACCAAGAGGCCCCCTCGCACCATTATTAATTGGCTTTCTCATTGCTAT
CATTGGCGGTGCGATGGGACCTTTAACTGGCTTTGCCATGAACCCCGCGCGCGATTTGGGACCCAAAGTCTTTGCTTCAC
TTGCCGGCTGGGGAGAAATCGCCCTGACAGGGGGAAGAGAAATACCTTATATTTTCATCCCGGTTATCGCCCCAATCCTG
GGTGGGCTTTTTGGTGGTTGGATTTATACCAAATTCGTCGGCAAAAACTTACCTTGTCATTGCGAATAA

Upstream 100 bases:

>100_bases
GTTTATTGTCGGAAATGACCGCTTTTCACATGATAAAAACGTTAACCAATCTCATACATTCACATTTATTTTCTACTACA
ATCCTGTTAGGAGTATCAAC

Downstream 100 bases:

>100_bases
TTCACGTTCATTTAAACAGAGGAAAAACATGATGACGGATAAAAAATATATCATTGCCCTAGATCAAGGTACAACAAGTT
CAAGAGCGGTTCTTCTTGAT

Product: hypothetical protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDKSLKAACIGEFLGTALLIFFGVGCVAALKVAGANFGQWEISIMWGLGVAIAVYASAGLSGAHLNPAVTIALWQFACFD
GKKVVPYILAQLAGAFCSAALVYFLYHNLFIDVEIAQQMVRGTPESLSLASTFSTYPHPSISVLHAFIVEFVITAVLMAL
ILALTDDGNGLPRGPLAPLLIGFLIAIIGGAMGPLTGFAMNPARDLGPKVFASLAGWGEIALTGGREIPYIFIPVIAPIL
GGLFGGWIYTKFVGKNLPCHCE

Sequences:

>Translated_262_residues
MDKSLKAACIGEFLGTALLIFFGVGCVAALKVAGANFGQWEISIMWGLGVAIAVYASAGLSGAHLNPAVTIALWQFACFD
GKKVVPYILAQLAGAFCSAALVYFLYHNLFIDVEIAQQMVRGTPESLSLASTFSTYPHPSISVLHAFIVEFVITAVLMAL
ILALTDDGNGLPRGPLAPLLIGFLIAIIGGAMGPLTGFAMNPARDLGPKVFASLAGWGEIALTGGREIPYIFIPVIAPIL
GGLFGGWIYTKFVGKNLPCHCE
>Mature_262_residues
MDKSLKAACIGEFLGTALLIFFGVGCVAALKVAGANFGQWEISIMWGLGVAIAVYASAGLSGAHLNPAVTIALWQFACFD
GKKVVPYILAQLAGAFCSAALVYFLYHNLFIDVEIAQQMVRGTPESLSLASTFSTYPHPSISVLHAFIVEFVITAVLMAL
ILALTDDGNGLPRGPLAPLLIGFLIAIIGGAMGPLTGFAMNPARDLGPKVFASLAGWGEIALTGGREIPYIFIPVIAPIL
GGLFGGWIYTKFVGKNLPCHCE

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=252, Percent_Identity=38.0952380952381, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI4826645, Length=261, Percent_Identity=35.632183908046, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI22538420, Length=260, Percent_Identity=36.5384615384615, Blast_Score=142, Evalue=2e-34,
Organism=Homo sapiens, GI4502187, Length=259, Percent_Identity=32.8185328185328, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI310133356, Length=207, Percent_Identity=29.951690821256, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI310114181, Length=207, Percent_Identity=29.951690821256, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI4502179, Length=245, Percent_Identity=33.0612244897959, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI6912506, Length=249, Percent_Identity=30.1204819277108, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI86792455, Length=251, Percent_Identity=30.6772908366534, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI4502181, Length=208, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4755125, Length=208, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790362, Length=258, Percent_Identity=70.1550387596899, Blast_Score=380, Evalue=1e-107,
Organism=Escherichia coli, GI1787101, Length=221, Percent_Identity=32.1266968325792, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994009, Length=263, Percent_Identity=35.361216730038, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI71992966, Length=253, Percent_Identity=35.5731225296443, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564052, Length=258, Percent_Identity=34.4961240310078, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17533613, Length=258, Percent_Identity=34.4961240310078, Blast_Score=131, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17531431, Length=263, Percent_Identity=31.1787072243346, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17531429, Length=263, Percent_Identity=31.1787072243346, Blast_Score=123, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17544068, Length=257, Percent_Identity=34.2412451361868, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71992961, Length=253, Percent_Identity=32.4110671936759, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17558372, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71993722, Length=261, Percent_Identity=27.2030651340996, Blast_Score=67, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6321054, Length=210, Percent_Identity=40, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=29.3906810035842, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24762344, Length=202, Percent_Identity=31.1881188118812, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762346, Length=202, Percent_Identity=31.1881188118812, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24762348, Length=202, Percent_Identity=31.1881188118812, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI20130305, Length=202, Percent_Identity=31.1881188118812, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652747, Length=248, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45551084, Length=248, Percent_Identity=30.6451612903226, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24762342, Length=215, Percent_Identity=30.6976744186047, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45550503, Length=202, Percent_Identity=28.2178217821782, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19922828, Length=200, Percent_Identity=30, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 27535; Mature: 27535

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLKAACIGEFLGTALLIFFGVGCVAALKVAGANFGQWEISIMWGLGVAIAVYASAGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCC
SGAHLNPAVTIALWQFACFDGKKVVPYILAQLAGAFCSAALVYFLYHNLFIDVEIAQQMV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGTPESLSLASTFSTYPHPSISVLHAFIVEFVITAVLMALILALTDDGNGLPRGPLAPLL
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IGFLIAIIGGAMGPLTGFAMNPARDLGPKVFASLAGWGEIALTGGREIPYIFIPVIAPIL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHHHHH
GGLFGGWIYTKFVGKNLPCHCE
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDKSLKAACIGEFLGTALLIFFGVGCVAALKVAGANFGQWEISIMWGLGVAIAVYASAGL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHCCC
SGAHLNPAVTIALWQFACFDGKKVVPYILAQLAGAFCSAALVYFLYHNLFIDVEIAQQMV
CCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGTPESLSLASTFSTYPHPSISVLHAFIVEFVITAVLMALILALTDDGNGLPRGPLAPLL
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
IGFLIAIIGGAMGPLTGFAMNPARDLGPKVFASLAGWGEIALTGGREIPYIFIPVIAPIL
HHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEEHHHHHHHHH
GGLFGGWIYTKFVGKNLPCHCE
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]