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The map label for this gene is crp

Identifier: 15603022

GI number: 15603022

Start: 1353273

End: 1353902

Strand: Direct

Name: crp

Synonym: PM1157

Alternate gene names: 15603022

Gene position: 1353273-1353902 (Clockwise)

Preceding gene: 15603021

Following gene: 15603023

Centisome position: 59.95

GC content: 38.73

Gene sequence:

>630_bases
ATGCAAACTACACCATCGATAGATCCTACGTTAGAGTGGTTTCTATCTCACTGCCATATTCACAAATATCCTTCCAAAAG
TACATTGATTCATGCAGGTGAAAAAGCCGAGACGCTGTATTATCTGATTAAAGGTTCCGTTGCTGTCTTAGTCAAAGATG
AAGATGGCAAAGAAATGATTTTGACTTATTTAAGTCAAGGTGATTTTTTTGGTGAGGCTGGTCTTTTCGAAGAGGGACAA
TTACGTTCAGCTTGGATTAAAGCAAAAAGCCCTTGTGAAATTGCTGAAATTTCTTATAAAAAATTTCGTCAATTAATTCA
GGTCAACCCAGATATTTTAATGCATTTATCAGCTCAGCTAGCACGTCGCTTACAAAATACGTCAAGACAAGTAAGTAATC
TAGCCTTTTTAGATGTGACGGGGCGTATCGCACAAACCTTATTAAATCTGGCTAAAATGCCAGAAGCCATGACCCATCCA
GATGGGATGCAAATTAAAATCACGCGCCAAGAAATTGGGCAAATGGTGGGATGTTCGCGTGAAACGGTAGGACGAATCCT
CAAAATGTTAGAAGATCAGCATTTAATCTCTGCACATGGTAAAACCATTGTCGTTTACGGTACAAGATAA

Upstream 100 bases:

>100_bases
ACTGCACGAATCAATTGATATAAAAGACAAAATGGACTTGTTAAAATGATTCTACTTTGTCGGAAAAATTAGTTTATGGA
GGTCTTCCGTGCAAGAACAA

Downstream 100 bases:

>100_bases
TCCCCGGTTCTCTGCTAAAATGCGATCCATTTGATCGCATTTTTTATATTCAAATGACCTGTTAATTCTCATTCTTATTT
TGATTTGAACATGGTAAGCT

Product: cAMP-regulatory protein

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MQTTPSIDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYLIKGSVAVLVKDEDGKEMILTYLSQGDFFGEAGLFEEGQ
LRSAWIKAKSPCEIAEISYKKFRQLIQVNPDILMHLSAQLARRLQNTSRQVSNLAFLDVTGRIAQTLLNLAKMPEAMTHP
DGMQIKITRQEIGQMVGCSRETVGRILKMLEDQHLISAHGKTIVVYGTR

Sequences:

>Translated_209_residues
MQTTPSIDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYLIKGSVAVLVKDEDGKEMILTYLSQGDFFGEAGLFEEGQ
LRSAWIKAKSPCEIAEISYKKFRQLIQVNPDILMHLSAQLARRLQNTSRQVSNLAFLDVTGRIAQTLLNLAKMPEAMTHP
DGMQIKITRQEIGQMVGCSRETVGRILKMLEDQHLISAHGKTIVVYGTR
>Mature_209_residues
MQTTPSIDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYLIKGSVAVLVKDEDGKEMILTYLSQGDFFGEAGLFEEGQ
LRSAWIKAKSPCEIAEISYKKFRQLIQVNPDILMHLSAQLARRLQNTSRQVSNLAFLDVTGRIAQTLLNLAKMPEAMTHP
DGMQIKITRQEIGQMVGCSRETVGRILKMLEDQHLISAHGKTIVVYGTR

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789756, Length=205, Percent_Identity=86.8292682926829, Blast_Score=381, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CRP_PASMU (O05689)

Other databases:

- EMBL:   U95380
- EMBL:   AE004439
- RefSeq:   NP_246094.1
- ProteinModelPortal:   O05689
- SMR:   O05689
- GeneID:   1244504
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1157
- NMPDR:   fig|272843.1.peg.1157
- HOGENOM:   HBG648099
- OMA:   DEGHEMI
- ProtClustDB:   PRK11753
- BioCyc:   PMUL272843:PM1157-MONOMER
- GO:   GO:0005622
- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.60.120.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00034
- SMART:   SM00100
- SMART:   SM00419

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp; SSF51206 cNMP_binding

EC number: NA

Molecular weight: Translated: 23542; Mature: 23542

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00888 CNMP_BINDING_1; PS00889 CNMP_BINDING_2; PS50042 CNMP_BINDING_3; PS00042 HTH_CRP_1; PS51063 HTH_CRP_2

Important sites: BINDING 128-128

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTTPSIDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYLIKGSVAVLVKDEDGKEMI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCHHHEEEEEECCEEEEEECCCCHHHH
LTYLSQGDFFGEAGLFEEGQLRSAWIKAKSPCEIAEISYKKFRQLIQVNPDILMHLSAQL
HHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ARRLQNTSRQVSNLAFLDVTGRIAQTLLNLAKMPEAMTHPDGMQIKITRQEIGQMVGCSR
HHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEHHHHHHHHCCCH
ETVGRILKMLEDQHLISAHGKTIVVYGTR
HHHHHHHHHHCCCHHHHCCCCEEEEEECC
>Mature Secondary Structure
MQTTPSIDPTLEWFLSHCHIHKYPSKSTLIHAGEKAETLYYLIKGSVAVLVKDEDGKEMI
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCHHHEEEEEECCEEEEEECCCCHHHH
LTYLSQGDFFGEAGLFEEGQLRSAWIKAKSPCEIAEISYKKFRQLIQVNPDILMHLSAQL
HHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHH
ARRLQNTSRQVSNLAFLDVTGRIAQTLLNLAKMPEAMTHPDGMQIKITRQEIGQMVGCSR
HHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCHHHCCCCCCEEEEEHHHHHHHHCCCH
ETVGRILKMLEDQHLISAHGKTIVVYGTR
HHHHHHHHHHCCCHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100