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The map label for this gene is rfaK [C]

Identifier: 15603009

GI number: 15603009

Start: 1343549

End: 1344580

Strand: Direct

Name: rfaK [C]

Synonym: PM1144

Alternate gene names: 15603009

Gene position: 1343549-1344580 (Clockwise)

Preceding gene: 15603008

Following gene: 15603018

Centisome position: 59.52

GC content: 33.24

Gene sequence:

>1032_bases
ATGAAATTAAAAAATAAATTACAAATGTTAAGGTTGGGTCTAGGCAAATATTTCCTTGATAAAAAAAACGGATTAAACAG
AATAACAAATGTTCCTAGAAGCATCCTCTTCCTCCGCCAAGACGGAAAAATTGGGGATTATGTGGTGAGCTCATTTGTAT
TCCGTGAGATAAAAAAATTTAATCCCCACATTAAAATTGGTGTAATTTGTACCAAACAAAATGCTTATCTTTTTAAACAA
AATCCATATATCGATCAACTTTACTATGTAAAAAAGAAAAGTATTTTGGATTACATCAAATGTGGTCTAGCAATTCAAAA
AGAACAATATGATTTAGTGATTGATCCGACGATTATGATTCGTAATCGCGATCTTTTACTTTTACGCTTAATCAATGCCA
AGCATTATATTGGCTACCAAAAAGCCAATTATGGTTTATTTAATATTAATCTGGAGGGACAATTTCACTTTTCGGAACTC
TATAAACTCGCCTTAGAAAAAGTGAATATTACGGTACAAGATATAAGCTATGACGTCCCATTTGATGAGCAAAGTGCGGT
CGAAATTTCTGAATTTTTACAGAAAAACCAACTAGAAAAGTATATTGCTATTAATTTTTATGGTGCTGCCAGAATCAAAA
AAGTAAACGATGACAACATCAAAAAATATTTAGATTATCTCACGCAAGTCAGCGGGGGAAAAAAGCTGGTGCTATTAAGC
TATCCTGAAGTAACAGAGAAATTAACACAATTGTCAGCCGATTATCCGCATATTTTTGTCCATCCAACAACCAAGATCTT
TCATACCATTGAATTGATTCGCCACTGTGATCAATTAATCTCTACAGACACGTCTACTGTACATATTGCTTCAGGTTTTA
ATAAGCCAATTATTGGTATTTATAAAGAAGATCCTATTGCGTTTACACATTGGCAACCCAAAAGTCAGGCAGAAACGCAC
ATACTTTTCTATAAAGAAAATATTAATGAGCTCTCACCTGAACAAATTGACCCTGCATGGCTTGTCAGATAG

Upstream 100 bases:

>100_bases
GTGCTTTACAATTAACTGGACTAAAGGCTTATGGTGTTGAACCACCTTGTGTATTTAAAGGAGCAATTTCAGAAATTGAT
GCAATGGAGCAACGCTAACA

Downstream 100 bases:

>100_bases
TCTTATCTCTTCTGACACTTGGGGCAATAGAAACTATTTCGTTGCCCTATCACTAAACTTTCTATTTTTGTGCCACATGT
TGGACAAGGCTTATCCTTAT

Product: hypothetical protein

Products: UDP; N-Acetyl-D-Glucosaminyl- Lipopolysaccharide. [C]

Alternate protein names: Lipooligosaccharide D-Glycero-D-Manno-Heptosyltransferase; RfaF Protein; Heptosyl Transferase; Heptosyltransferase II; Los Biosynthesis Lbgb; Lipopolysaccharide 1 2-N-Acetylglucosaminetransferase RfaK; ADP-HeptoseLPS Heptosyltransferase; Lipopolysaccharide Heptosyltransferase II; D-Glycero-D-Manno-Heptosyl Transferase; Lipooligosaccharided-Glycero-D-Manno-Heptosyltransferase; Heptosyltransferase; Glycosyl Transferase

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKLKNKLQMLRLGLGKYFLDKKNGLNRITNVPRSILFLRQDGKIGDYVVSSFVFREIKKFNPHIKIGVICTKQNAYLFKQ
NPYIDQLYYVKKKSILDYIKCGLAIQKEQYDLVIDPTIMIRNRDLLLLRLINAKHYIGYQKANYGLFNINLEGQFHFSEL
YKLALEKVNITVQDISYDVPFDEQSAVEISEFLQKNQLEKYIAINFYGAARIKKVNDDNIKKYLDYLTQVSGGKKLVLLS
YPEVTEKLTQLSADYPHIFVHPTTKIFHTIELIRHCDQLISTDTSTVHIASGFNKPIIGIYKEDPIAFTHWQPKSQAETH
ILFYKENINELSPEQIDPAWLVR

Sequences:

>Translated_343_residues
MKLKNKLQMLRLGLGKYFLDKKNGLNRITNVPRSILFLRQDGKIGDYVVSSFVFREIKKFNPHIKIGVICTKQNAYLFKQ
NPYIDQLYYVKKKSILDYIKCGLAIQKEQYDLVIDPTIMIRNRDLLLLRLINAKHYIGYQKANYGLFNINLEGQFHFSEL
YKLALEKVNITVQDISYDVPFDEQSAVEISEFLQKNQLEKYIAINFYGAARIKKVNDDNIKKYLDYLTQVSGGKKLVLLS
YPEVTEKLTQLSADYPHIFVHPTTKIFHTIELIRHCDQLISTDTSTVHIASGFNKPIIGIYKEDPIAFTHWQPKSQAETH
ILFYKENINELSPEQIDPAWLVR
>Mature_343_residues
MKLKNKLQMLRLGLGKYFLDKKNGLNRITNVPRSILFLRQDGKIGDYVVSSFVFREIKKFNPHIKIGVICTKQNAYLFKQ
NPYIDQLYYVKKKSILDYIKCGLAIQKEQYDLVIDPTIMIRNRDLLLLRLINAKHYIGYQKANYGLFNINLEGQFHFSEL
YKLALEKVNITVQDISYDVPFDEQSAVEISEFLQKNQLEKYIAINFYGAARIKKVNDDNIKKYLDYLTQVSGGKKLVLLS
YPEVTEKLTQLSADYPHIFVHPTTKIFHTIELIRHCDQLISTDTSTVHIASGFNKPIIGIYKEDPIAFTHWQPKSQAETH
ILFYKENINELSPEQIDPAWLVR

Specific function: Adds The Terminal N-Acetyl-D-Glucosamine Group On The Glucose(II) Group Of Lps. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Peripheral Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.4.1.56 [C]

Molecular weight: Translated: 39991; Mature: 39991

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNKLQMLRLGLGKYFLDKKNGLNRITNVPRSILFLRQDGKIGDYVVSSFVFREIKKF
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
NPHIKIGVICTKQNAYLFKQNPYIDQLYYVKKKSILDYIKCGLAIQKEQYDLVIDPTIMI
CCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCEEEE
RNRDLLLLRLINAKHYIGYQKANYGLFNINLEGQFHFSELYKLALEKVNITVQDISYDVP
ECCCEEEEEEECCHHHCCEEECCCCEEEEEECCEECHHHHHHHHHHHHEEEEEEEEECCC
FDEQSAVEISEFLQKNQLEKYIAINFYGAARIKKVNDDNIKKYLDYLTQVSGGKKLVLLS
CCCCHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEE
YPEVTEKLTQLSADYPHIFVHPTTKIFHTIELIRHCDQLISTDTSTVHIASGFNKPIIGI
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
YKEDPIAFTHWQPKSQAETHILFYKENINELSPEQIDPAWLVR
EECCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCC
>Mature Secondary Structure
MKLKNKLQMLRLGLGKYFLDKKNGLNRITNVPRSILFLRQDGKIGDYVVSSFVFREIKKF
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHC
NPHIKIGVICTKQNAYLFKQNPYIDQLYYVKKKSILDYIKCGLAIQKEQYDLVIDPTIMI
CCCEEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCEEECCEEEE
RNRDLLLLRLINAKHYIGYQKANYGLFNINLEGQFHFSELYKLALEKVNITVQDISYDVP
ECCCEEEEEEECCHHHCCEEECCCCEEEEEECCEECHHHHHHHHHHHHEEEEEEEEECCC
FDEQSAVEISEFLQKNQLEKYIAINFYGAARIKKVNDDNIKKYLDYLTQVSGGKKLVLLS
CCCCHHHHHHHHHHHHCCCEEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCCEEEEEE
YPEVTEKLTQLSADYPHIFVHPTTKIFHTIELIRHCDQLISTDTSTVHIASGFNKPIIGI
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEE
YKEDPIAFTHWQPKSQAETHILFYKENINELSPEQIDPAWLVR
EECCCEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Lipopolysaccharide [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Lipopolysaccharide = UDP + N-Acetyl-D-Glucosaminyl- Lipopolysaccharide. [C]

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA